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Conserved domains on  [gi|194097425|ref|NP_775949|]
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TRPM8 channel-associated factor 2 isoform A [Homo sapiens]

Protein Classification

M60 family metallopeptidase( domain architecture ID 13879573)

M60 family metallopeptidase similar to enhancin, a peptidase from granulosis viruses that affects Lepidoptera, and increases the potency of viral infection

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-835 7.22e-81

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


:

Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 261.16  E-value: 7.22e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  633 GAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKD--PEPVLRLWDEMMQAVARLAAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDRlnPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  707 PFRRPERIVADVQISAGWMHSGYPIMCH-LESVKEIINEMDMRSRGVWGPIHELGHNQQ-RHGWEFPPHTTEATCNLWSV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  785 YVHETVLGI---------PRAQAHEALSPPERERRIKAHLGKGAPLcdwnvwtaLETYLQ 835
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQ 212
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-632 7.41e-36

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


:

Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 130.92  E-value: 7.41e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  522 LYPSKHPITVEINGinpgNNDCWVSTGLYLLEGQNAEVSLSEAAAsAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDR 601
Cdd:pfam17291   1 NNATREKARVRINS----RYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|.
gi 194097425  602 TERSVSCLWGGLLYVIVPKGSQLGPVPVTIR 632
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTIS 106
 
Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-835 7.22e-81

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 261.16  E-value: 7.22e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  633 GAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKD--PEPVLRLWDEMMQAVARLAAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDRlnPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  707 PFRRPERIVADVQISAGWMHSGYPIMCH-LESVKEIINEMDMRSRGVWGPIHELGHNQQ-RHGWEFPPHTTEATCNLWSV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  785 YVHETVLGI---------PRAQAHEALSPPERERRIKAHLGKGAPLcdwnvwtaLETYLQ 835
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQ 212
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-632 7.41e-36

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 130.92  E-value: 7.41e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  522 LYPSKHPITVEINGinpgNNDCWVSTGLYLLEGQNAEVSLSEAAAsAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDR 601
Cdd:pfam17291   1 NNATREKARVRINS----RYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|.
gi 194097425  602 TERSVSCLWGGLLYVIVPKGSQLGPVPVTIR 632
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTIS 106
SslE_AcfD_Zn_LP NF037974
SslE/AcfD family lipoprotein zinc metalloprotease; Members of this family are surface ...
 529-794 3.93e-14

SslE/AcfD family lipoprotein zinc metalloprotease; Members of this family are surface lipoprotein zinc metalloproteases, from the family that includes accessory colonization factor AcfD from Vibrio cholerae, SslE (YghJ ) from E. coli (Secreted and Surface-associated Lipoprotein from E. coli), and VPA1376 from Vibrio parahaemolyticus. Each is about 1500 amino acids long, and SslE is a known substrate of a type II secretion system (T2SS). SslE is known to have mucinase activity.


Pssm-ID: 468296 [Multi-domain]  Cd Length: 1389  Bit Score: 76.96  E-value: 3.93e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  529 ITVEINGiNP-----GNNDcwvSTGLYLLEGQNAEVSLSeaaASAGLRVQIgchTDDLTKARK----LSRAPVVThQCW- 598
Cdd:NF037974  951 VTIDTNN-NTvayfaGNRQ---STGLWAPALQEVTVSGG---GEATITVAL---ADDLTGREKhelsLKRPPRMQ-KSFv 1019

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  599 -MDRTERSVSCLWGGLLYvIVPKGSQLGPVPVTIRGAVPAPYYKLGKtsleeWKRQmQENLAPWGELATDNIILTVPTTN 677
Cdd:NF037974 1020 yDGGSSLTFTVPYGGLIY-IQPGNSESVESTFSFSGVVKAPLWKNGQ-----WVNP-LNSPAPIAEIDSGSFIYTTPKAN 1092

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  678 LQALkDPEpvlRLWDEMMQAVArlAAEPFPFR----------------RPE---RIVADVQISAGWMHSGYPIMCHLESV 738
Cdd:NF037974 1093 LNAS-DIA---QFAKDLNRFAE--DASDFYGRdegtsdgkhrrftysaLPNnrhRFVNDVQISIGAAHSGYPVMNSSFNA 1166

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  739 K-EIINEMDMRSRGVWgpiHELGHNQQrhgwEFP---PHTTEATCNLWSVYVHETVLGIP 794
Cdd:NF037974 1167 DsTSIPTTPLNDWLLW---HEVGHNLA----EAPfnvEGATEVTNNLLALYMQELRLGNP 1219
 
Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-835 7.22e-81

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 261.16  E-value: 7.22e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  633 GAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKD--PEPVLRLWDEMMQAVARLAAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDRlnPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  707 PFRRPERIVADVQISAGWMHSGYPIMCH-LESVKEIINEMDMRSRGVWGPIHELGHNQQ-RHGWEFPPHTTEATCNLWSV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  785 YVHETVLGI---------PRAQAHEALSPPERERRIKAHLGKGAPLcdwnvwtaLETYLQ 835
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQ 212
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-632 7.41e-36

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 130.92  E-value: 7.41e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  522 LYPSKHPITVEINGinpgNNDCWVSTGLYLLEGQNAEVSLSEAAAsAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDR 601
Cdd:pfam17291   1 NNATREKARVRINS----RYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|.
gi 194097425  602 TERSVSCLWGGLLYVIVPKGSQLGPVPVTIR 632
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTIS 106
SslE_AcfD_Zn_LP NF037974
SslE/AcfD family lipoprotein zinc metalloprotease; Members of this family are surface ...
 529-794 3.93e-14

SslE/AcfD family lipoprotein zinc metalloprotease; Members of this family are surface lipoprotein zinc metalloproteases, from the family that includes accessory colonization factor AcfD from Vibrio cholerae, SslE (YghJ ) from E. coli (Secreted and Surface-associated Lipoprotein from E. coli), and VPA1376 from Vibrio parahaemolyticus. Each is about 1500 amino acids long, and SslE is a known substrate of a type II secretion system (T2SS). SslE is known to have mucinase activity.


Pssm-ID: 468296 [Multi-domain]  Cd Length: 1389  Bit Score: 76.96  E-value: 3.93e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  529 ITVEINGiNP-----GNNDcwvSTGLYLLEGQNAEVSLSeaaASAGLRVQIgchTDDLTKARK----LSRAPVVThQCW- 598
Cdd:NF037974  951 VTIDTNN-NTvayfaGNRQ---STGLWAPALQEVTVSGG---GEATITVAL---ADDLTGREKhelsLKRPPRMQ-KSFv 1019

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  599 -MDRTERSVSCLWGGLLYvIVPKGSQLGPVPVTIRGAVPAPYYKLGKtsleeWKRQmQENLAPWGELATDNIILTVPTTN 677
Cdd:NF037974 1020 yDGGSSLTFTVPYGGLIY-IQPGNSESVESTFSFSGVVKAPLWKNGQ-----WVNP-LNSPAPIAEIDSGSFIYTTPKAN 1092

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  678 LQALkDPEpvlRLWDEMMQAVArlAAEPFPFR----------------RPE---RIVADVQISAGWMHSGYPIMCHLESV 738
Cdd:NF037974 1093 LNAS-DIA---QFAKDLNRFAE--DASDFYGRdegtsdgkhrrftysaLPNnrhRFVNDVQISIGAAHSGYPVMNSSFNA 1166

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 194097425  739 K-EIINEMDMRSRGVWgpiHELGHNQQrhgwEFP---PHTTEATCNLWSVYVHETVLGIP 794
Cdd:NF037974 1167 DsTSIPTTPLNDWLLW---HEVGHNLA----EAPfnvEGATEVTNNLLALYMQELRLGNP 1219
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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