phosphoacceptor receiver (REC) domain of type B Arabidopsis response regulators (ARRs) and similar domains; Type-B ARRs (Arabidopsis response regulators) are a class of MYB-type transcription factors that act as major players in the transcriptional activation of cytokinin-responsive genes. They directly regulate the expression of type-A ARR genes and other downstream target genes. Cytokinin is a plant hormone implicated in many growth and development processes including shoot organogenesis, leaf senescence, sink/source relationships, vascular development, lateral bud release, and photomorphogenic development. Cytokinin signaling involves a phosphorelay cascade by histidine kinase receptors (AHKs), histidine phosphotransfer proteins (AHPs) and downstream ARRs. ARRs are divided into two groups, type-A and -B, according to their sequence and domain structure. Type-B ARRs contain a receiver (REC) domain and a large C-terminal extension that has characteristics of an effector or output domain, with a Myb-like DNA binding domain referred to as the GARP domain. The GARP domain is a motif specific to plant transcription factors. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  26 GCDSDGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPESFHIAIVEVNMSAEsESFKFLEAAKDV--LPTIMIST 103
Cdd:cd17584   1 VLVVDDDPTCLAILKRMLLRCGYQVTTCTDAEEALSMLRENKDEFDLVITDVHMPDM-DGFEFLELIRLEmdLPVIMMSA 79

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 30684266 104 DHCITTTMKCIALGAVEFLQKPLSPEKLKNIWQHVV 139
Cdd:cd17584  80 DGSTSTVMKGLAHGACDYLLKPVSIEDLKNIWQHVV 115

phosphoacceptor receiver (REC) domain of type B Arabidopsis response regulators (ARRs) and similar domains; Type-B ARRs (Arabidopsis response regulators) are a class of MYB-type transcription factors that act as major players in the transcriptional activation of cytokinin-responsive genes. They directly regulate the expression of type-A ARR genes and other downstream target genes. Cytokinin is a plant hormone implicated in many growth and development processes including shoot organogenesis, leaf senescence, sink/source relationships, vascular development, lateral bud release, and photomorphogenic development. Cytokinin signaling involves a phosphorelay cascade by histidine kinase receptors (AHKs), histidine phosphotransfer proteins (AHPs) and downstream ARRs. ARRs are divided into two groups, type-A and -B, according to their sequence and domain structure. Type-B ARRs contain a receiver (REC) domain and a large C-terminal extension that has characteristics of an effector or output domain, with a Myb-like DNA binding domain referred to as the GARP domain. The GARP domain is a motif specific to plant transcription factors. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  26 GCDSDGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPESFHIAIVEVNMSAEsESFKFLEAAKDV--LPTIMIST 103
Cdd:cd17584   1 VLVVDDDPTCLAILKRMLLRCGYQVTTCTDAEEALSMLRENKDEFDLVITDVHMPDM-DGFEFLELIRLEmdLPVIMMSA 79

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 30684266 104 DHCITTTMKCIALGAVEFLQKPLSPEKLKNIWQHVV 139
Cdd:cd17584  80 DGSTSTVMKGLAHGACDYLLKPVSIEDLKNIWQHVV 115

myb-like DNA-binding domain, SHAQKYF class; This model describes a DNA-binding domain restricted to (but common in) plant proteins, many of which also contain a response regulator domain. The domain appears related to the Myb-like DNA-binding domain described by pfam00249. It is distinguished in part by a well-conserved motif SH[AL]QKY[RF] at the C-terminal end of the motif.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 30684266   296 RKKVDWTPELHKKFVQAVEQLG-VDQAIPSRILELMKVGTLTRHNVASHLQKFRQHR 351
Cdd:TIGR01557   1 KPRVVWTEDLHDRFLQAVQKLGgPDWATPKRILELMVVDGLTRDQVASHLQKYRLKQ 57

Response regulator receiver domain; This domain receives the signal from the sensor partner in bacterial two-component systems. It is usually found N-terminal to a DNA binding effector domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266    40 RSELESMDYIVTTFTDETEALSAVvkNPESFHIAIVEVNMSAES--ESFKFLEAAKDVLPTIMISTDHCITTTMKCIALG 117
Cdd:pfam00072  15 RQLLEKEGYVVAEADDGKEALELL--KEERPDLILLDINMPGMDglELLKRIRRRDPTTPVIILTAHGDEDDAVEALEAG 92

                          90
                  ....*....|....*..
gi 30684266   118 AVEFLQKPLSPEKLKNI 134
Cdd:pfam00072  93 ADDFLSKPFDPDELLAA 109

phosphoacceptor receiver (REC) domain of type B Arabidopsis response regulators (ARRs) and similar domains; Type-B ARRs (Arabidopsis response regulators) are a class of MYB-type transcription factors that act as major players in the transcriptional activation of cytokinin-responsive genes. They directly regulate the expression of type-A ARR genes and other downstream target genes. Cytokinin is a plant hormone implicated in many growth and development processes including shoot organogenesis, leaf senescence, sink/source relationships, vascular development, lateral bud release, and photomorphogenic development. Cytokinin signaling involves a phosphorelay cascade by histidine kinase receptors (AHKs), histidine phosphotransfer proteins (AHPs) and downstream ARRs. ARRs are divided into two groups, type-A and -B, according to their sequence and domain structure. Type-B ARRs contain a receiver (REC) domain and a large C-terminal extension that has characteristics of an effector or output domain, with a Myb-like DNA binding domain referred to as the GARP domain. The GARP domain is a motif specific to plant transcription factors. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  26 GCDSDGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPESFHIAIVEVNMSAEsESFKFLEAAKDV--LPTIMIST 103
Cdd:cd17584   1 VLVVDDDPTCLAILKRMLLRCGYQVTTCTDAEEALSMLRENKDEFDLVITDVHMPDM-DGFEFLELIRLEmdLPVIMMSA 79

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 30684266 104 DHCITTTMKCIALGAVEFLQKPLSPEKLKNIWQHVV 139
Cdd:cd17584  80 DGSTSTVMKGLAHGACDYLLKPVSIEDLKNIWQHVV 115

myb-like DNA-binding domain, SHAQKYF class; This model describes a DNA-binding domain restricted to (but common in) plant proteins, many of which also contain a response regulator domain. The domain appears related to the Myb-like DNA-binding domain described by pfam00249. It is distinguished in part by a well-conserved motif SH[AL]QKY[RF] at the C-terminal end of the motif.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 30684266   296 RKKVDWTPELHKKFVQAVEQLG-VDQAIPSRILELMKVGTLTRHNVASHLQKFRQHR 351
Cdd:TIGR01557   1 KPRVVWTEDLHDRFLQAVQKLGgPDWATPKRILELMVVDGLTRDQVASHLQKYRLKQ 57

Response regulator c-di-GMP phosphodiesterase, RpfG family, contains REC and HD-GYP domains [Signal transduction mechanisms];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  43 LESMDYIVTTFTDETEALSAVVKNPesFHIAIVEVNMSAES-----ESFKFLEAAKDVlPTIMISTDHCITTTMKCIALG 117
Cdd:COG3437  26 LRTLGYDVVTAESGEEALELLLEAP--PDLILLDVRMPGMDgfellRLLRADPSTRDI-PVIFLTALADPEDRERALEAG 102

                        90
                ....*....|....*
gi 30684266 118 AVEFLQKPLSPEKLK 132
Cdd:COG3437 103 ADDYLTKPFDPEELL 117

Response regulator receiver domain; This domain receives the signal from the sensor partner in bacterial two-component systems. It is usually found N-terminal to a DNA binding effector domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266    40 RSELESMDYIVTTFTDETEALSAVvkNPESFHIAIVEVNMSAES--ESFKFLEAAKDVLPTIMISTDHCITTTMKCIALG 117
Cdd:pfam00072  15 RQLLEKEGYVVAEADDGKEALELL--KEERPDLILLDINMPGMDglELLKRIRRRDPTTPVIILTAHGDEDDAVEALEAG 92

                          90
                  ....*....|....*..
gi 30684266   118 AVEFLQKPLSPEKLKNI 134
Cdd:pfam00072  93 ADDFLSKPFDPDELLAA 109

phosphoacceptor receiver (REC) domain of response regulators (RRs) and pseudo response regulators (PRRs); Two-component systems (TCSs) involving a sensor and a response regulator are used by bacteria to adapt to changing environments. Processes regulated by two-component systems in bacteria include sporulation, pathogenicity, virulence, chemotaxis, and membrane transport. Response regulators (RRs) share the common phosphoacceptor REC domain and different effector/output domains such as DNA, RNA, ligand-binding, protein-binding, or enzymatic domains. Response regulators regulate transcription, post-transcription or post-translation, or have functions such as methylesterases, adenylate or diguanylate cyclase, c-di-GMP-specific phosphodiesterases, histidine kinases, serine/threonine protein kinases, and protein phosphatases, depending on their output domains. The function of some output domains are still unknown. TCSs are found in all three domains of life - bacteria, archaea, and eukaryotes, however, the presence and abundance of particular RRs vary between the lineages. Archaea encode very few RRs with DNA-binding output domains; most are stand-alone REC domains. Among eukaryotes, TCSs are found primarily in protozoa, fungi, algae, and green plants. REC domains function as phosphorylation-mediated switches within RRs, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  30 DGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPesFHIAIVEVNMSAESeSFKFLEAAKDV---LPTIMISTDHC 106
Cdd:cd00156   4 DDDPAIRELLKSLLEREGYEVDTAADGEEALELLREER--PDLVLLDLMMPGMD-GLELLRKLRELppdIPVIVLTAKAD 80

                        90
                ....*....|....*....
gi 30684266 107 ITTTMKCIALGAVEFLQKP 125
Cdd:cd00156  81 EEDAVRALELGADDYLVKP 99

DNA-binding response regulator, FixJ family, consists of REC and HTH domains [Signal transduction mechanisms, Transcription];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  30 DGDGSSAAETRSELESMDYIVTTFTDETEALSAVvkNPESFHIAIVEVNMSAES--ESFKFLEAAKDVLPTIMIsTDHC- 106
Cdd:COG4566   6 DDDEAVRDSLAFLLESAGLRVETFASAEAFLAAL--DPDRPGCLLLDVRMPGMSglELQEELAARGSPLPVIFL-TGHGd 82

                        90       100
                ....*....|....*....|....*
gi 30684266 107 ITTTMKCIALGAVEFLQKPLSPEKL 131
Cdd:COG4566  83 VPMAVRAMKAGAVDFLEKPFDDQAL 107

phosphoacceptor receiver (REC) domain of nitrogen assimilation regulatory protein NtrX and similar proteins; NtrX is part of the two-component regulatory system NtrY/NtrX that is involved in the activation of nitrogen assimilatory genes such as Gln. It is phosphorylated by the histidine kinase NtrY and interacts with sigma-54. NtrX is a member of the NtrC family, characterized by a domain architecture containing an N-terminal REC domain, followed by a central sigma-54 interaction/ATPase domain, and a C-terminal DNA binding domain. NtrC family response regulators are sigma54-dependent transcriptional activators. Also included in this subfamily is Aquifex aeolicus NtrC4. The ability of the central domain to hydrolyze ATP and thus to interact effectively with a complex of RNA polymerase, sigma54, and promoter, is controlled by the phosphorylation status of the REC domain. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  48 YIVTTFTDETEALSAVvkNPESFHIAIVEVNMsAESESFKFLEAAKDV---LPTIMISTDHCITTTMKCIALGAVEFLQK 124
Cdd:cd17550  23 YEVDTAADGEEALKLI--KERRPDLVLLDIWL-PDMDGLELLKEIKEKypdLPVIMISGHGTIETAVKATKLGAYDFIEK 99

                        90
                ....*....|....*
gi 30684266 125 PLSPEKLKNIWQHVV 139
Cdd:cd17550 100 PLSLDRLLLTIERAL 114

MAPK-interacting and spindle-stabilising protein-like; MISS is a family of eukaryotic MAPK-interacting and spindle-stabilising protein-like proteins. MISS is rich in prolines and has four potential MAPK-phosphorylation sites, a MAPK-docking site, a PEST sequence (PEST motif) and a bipartite nuclear localization signal. The endogenous protein accumulates during mouse meiotic maturation and is found as discrete dots on the MII spindle. MISS is the first example of a physiological MAPK-substrate that is stabilized in MII that specifically regulates MII spindle integrity during the CSF arrest.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266   383 QQHRPVMAYPvwglpgvyPPGAIPPlwPPPLQSIGQPPPwhwkPPYPTVSGNAWGCPVG-PPVTGSYITPS--NTTAGGF 459
Cdd:pfam15822 156 QYPAPNMPYP--------SPGPYPA--VPPPQSPGAAPP----VPWGTVPPGPWGPPAPyPDPTGSYPMPGlyPTPNNPF 221

                          90
                  ....*....|....
gi 30684266   460 QYPNGAeTGFKIMP 473
Cdd:pfam15822 222 QVPSGP-SGAPPMP 234

DNA-binding response regulator, ActR/RegA family, consists of REC and Fis-type HTH domains [Signal transduction mechanisms, Transcription];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  19 KVLLLLngcdsDGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPesFHIAIVEVNMSAESeSFKFLEAAKDVLP- 97
Cdd:COG4567   5 RSLLLV-----DDDEAFARVLARALERRGFEVTTAASVEEALALLEQAP--PDYAVLDLRLGDGS-GLDLIEALRERDPd 76

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 30684266  98 -TIMISTDH-CITTTMKCIALGAVEFLQKPLSPEKL 131
Cdd:COG4567  77 aRIVVLTGYaSIATAVEAIKLGADDYLAKPADADDL 112

phosphoacceptor receiver (REC) domain of FixJ family response regulators; FixJ family response regulators contain an N-terminal receiver domain (REC) and a C-terminal LuxR family helix-turn-helix (HTH) DNA-binding output domain. The Sinorhizobium meliloti two-component system FixL/FixJ regulates nitrogen fixation in response to oxygen during symbiosis. Under microaerobic conditions, the kinase FixL phosphorylates the response regulator FixJ resulting in the regulation of nitrogen fixation genes such as nifA and fixK. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  43 LESMDYIVTTFTDETEALSAVvkNPESFHIAIVEVNMSAES--ESFKFLEAAKDVLPTIMISTDHCITTTMKCIALGAVE 120
Cdd:cd17537  20 LRSVGLAVKTFTSASAFLAAA--PPDQPGCLVLDVRMPGMSglELQDELLARGSNIPIIFITGHGDVPMAVEAMKAGAVD 97

                        90
                ....*....|.
gi 30684266 121 FLQKPLSPEKL 131
Cdd:cd17537  98 FLEKPFRDQVL 108

phosphoacceptor receiver (REC) domain of plant ethylene receptors ETR1, ETR2, and EIN4, and similar proteins; Plant ethylene receptors contain N-terminal transmembrane domains that contain an ethylene binding site and also serve in localization of the receptor to the endoplasmic reticulum or the Golgi apparatus and a C-terminal histidine kinase (HK)-like domain. There are five ethylene receptors (ETR1, ERS1, ETR2, ERS2, and EIN4) in Arabidopsis thaliana. ETR1, ETR2, and EIN4 also contain REC domains C-terminal to the HK domain. ETR1 and ERS1 belong to subfamily 1, and have functional HK domains while ETR2, ERS2, and EIN4 belong to subfamily 2, and lack the necessary residues for HK activity and may function as serine/threonine kinases. The plant hormone ethylene plays an important role in plant growth and development. It regulates seed germination, seedling growth, leaf and petal abscission, fruit ripening, organ senescence, and pathogen responses. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  18 LKVLLLlngcdsDGDGSSAAETRSELESMDYIVTTFTDETEALSAVVKNPESFHIAIVEVNMsAESESFKFLEAAKDVL- 96
Cdd:cd19933   1 LKVLLV------DDNAVNRMVTKGLLEKLGCEVTTVSSGEECLNLLASAEHSFQLVLLDLCM-PEMDGFEVALRIRKLFg 73

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....
gi 30684266  97 ----PTIMI---STDHciTTTMKCIALGAVEFLQKPLSPEKLKN 133
Cdd:cd19933  74 rrerPLIVAltaNTDD--STREKCLSLGMNGVITKPVSLHALGD 115

Two-component response regulator, YesN/AraC family, consists of REC and AraC-type DNA-binding domains [Signal transduction mechanisms, Transcription];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30684266  49 IVTTFTDETEALSAVVKNPesFHIAIVEVNMSAESEsFKFLEAAKDVLP---TIMISTDHCITTTMKCIALGAVEFLQKP 125
Cdd:COG4753  27 VVGEAENGEEALELLEEHK--PDLVITDINMPGMDG-LELLEAIRELDPdtkIIILSGYSDFEYAQEAIKLGADDYLLKP 103

phosphoacceptor receiver (REC) domain of chemotaxis response regulator protein-glutamate methylesterase CheB and similar chemotaxis proteins; Methylesterase CheB is a chemotaxis response regulator with an N-terminal REC domain and a C-terminal methylesterase domain. Chemotaxis is a behavior known in motile bacteria that directs their movement in response to chemical gradients. CheB is a phosphorylation-activated response regulator involved in the reversible modification of bacterial chemotaxis receptors. It catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR. The CheB REC domain packs against the active site of the C-terminal domain and inhibits methylesterase activity by directly restricting access to the active site. Also included in this family is chemotaxis response regulator CheY, which contains a stand-alone REC domain, and an uncharacterized subfamily composed of proteins containing an N-terminal REC domain and a C-terminal CheY-P phosphatase (CheC) domain. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.

                        10        20        30
                ....*....|....*....|....*....|..
gi 30684266  96 LPTIMIS--TDHCITTTMKCIALGAVEFLQKP 125
Cdd:cd17541  74 TPVVMVSslTEEGAEITLEALELGAVDFIAKP 105