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Conserved domains on  [gi|225690560|ref|NP_998318|]
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deoxyribonuclease-1-like 1 [Danio rerio]

Protein Classification

exonuclease/endonuclease/phosphatase family protein( domain architecture ID 10178909)

endonuclease/exonuclease/phosphatase (EEP) family protein is among a diverse set of enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins; similar to Danio rerio deoxyribonuclease-1-like 1

CATH:  3.60.10.10
EC:  3.1.21.-
Gene Ontology:  GO:0046872|GO:0003677|GO:0006308|GO:0004536
PubMed:  10838565
SCOP:  4002213

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DNase1 cd10282
Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a ...
 25-283 3.05e-152

Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib, and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma) . DNASE1L3 is implicated in apoptotic DNA fragmentation. DNase I is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


:

Pssm-ID: 197337 [Multi-domain]  Cd Length: 256  Bit Score: 427.04  E-value: 3.05e-152
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  25 RICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDNDFQYVASERLGRTqTYQEQY 104
Cdd:cd10282    1 RIAAFNIQVFGESKMSKPEVMDVLVKILSRYDIVLIQEIRDSSGTAIPELLDELNSASSNTYSYVVSERLGRS-SYKEQY 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 105 VFFYRKSTAILKGQYQYPDTQTGDiDAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWETE 184
Cdd:cd10282   80 AFIYRSDKVSVLESYQYDDGDEGT-DVFSREPFVVRFSSPSTAVKDFVLVPIHTSPDDAVAEIDALYDVYDDVKQRWRED 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 185 NVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTVKeSTDCAYDRIVVHGEPFLRSIKPFSAQPFNFPKEFL 264
Cdd:cd10282  159 DVILLGDFNADCSYVTSKGWKSIRLRTDSRFHWLIGDDADTTVR-STNCAYDRIVVAGSLLQSAVVPGSAGVFDFDKEFG 237

                        250
                 ....*....|....*....
gi 225690560 265 LTEAQALEVSDHYPIEVVL 283
Cdd:cd10282  238 LTEEEALAVSDHYPVEVEL 256
 
Name Accession Description Interval E-value
DNase1 cd10282
Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a ...
 25-283 3.05e-152

Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib, and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma) . DNASE1L3 is implicated in apoptotic DNA fragmentation. DNase I is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197337 [Multi-domain]  Cd Length: 256  Bit Score: 427.04  E-value: 3.05e-152
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  25 RICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDNDFQYVASERLGRTqTYQEQY 104
Cdd:cd10282    1 RIAAFNIQVFGESKMSKPEVMDVLVKILSRYDIVLIQEIRDSSGTAIPELLDELNSASSNTYSYVVSERLGRS-SYKEQY 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 105 VFFYRKSTAILKGQYQYPDTQTGDiDAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWETE 184
Cdd:cd10282   80 AFIYRSDKVSVLESYQYDDGDEGT-DVFSREPFVVRFSSPSTAVKDFVLVPIHTSPDDAVAEIDALYDVYDDVKQRWRED 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 185 NVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTVKeSTDCAYDRIVVHGEPFLRSIKPFSAQPFNFPKEFL 264
Cdd:cd10282  159 DVILLGDFNADCSYVTSKGWKSIRLRTDSRFHWLIGDDADTTVR-STNCAYDRIVVAGSLLQSAVVPGSAGVFDFDKEFG 237

                        250
                 ....*....|....*....
gi 225690560 265 LTEAQALEVSDHYPIEVVL 283
Cdd:cd10282  238 LTEEEALAVSDHYPVEVEL 256
DNaseIc smart00476
deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of ...
 5-284 6.64e-137

deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of double-stranded DNA. The enzyme is secreted outside the cell and also involved in apoptosis in the nucleus.


Pssm-ID: 128752  Cd Length: 276  Bit Score: 388.72  E-value: 6.64e-137
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560     5 IRRILLSIFLLSGLLTcygFRICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDN 84
Cdd:smart00476   2 VPSLLLFLLLLHGAAS---LRICAFNIQSFGDSKMSNATLMSIIVKILSRYDIALVQEVRDSDLSAVPKLMDQLNSDSPN 78

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560    85 DFQYVASERLGRTqTYQEQYVFFYRKSTAILKGQYQYPDTQTGDIDAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNAT 164
Cdd:smart00476  79 TYSYVSSEPLGRN-SYKEQYLFLYRSDLVSVLDSYLYDDGCECGNDVFSREPFVVKFSSPSTAVKEFVIVPLHTTPEAAV 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560   165 KEIDELYDVFVDVKVRWETENVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTVKeSTDCAYDRIVVHGEP 244
Cdd:smart00476 158 AEIDALYDVYLDVRQKWGTEDVIFMGDFNAGCSYVTKKQWSSIRLRTSPTFHWLIPDSADTTVT-STHCAYDRIVVAGER 236

                          250       260       270       280
                   ....*....|....*....|....*....|....*....|
gi 225690560   245 FLRSIKPFSAQPFNFPKEFLLTEAQALEVSDHYPIEVVLK 284
Cdd:smart00476 237 LRSSVVPGSAAVFDFQTAYGLTEEEALAISDHFPVEVTLK 276
Exo_endo_phos pfam03372
Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium ...
 29-194 2.17e-10

Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signalling. This family includes: AP endonuclease proteins EC:4.2.99.18, DNase I proteins EC:3.1.21.1, Synaptojanin an inositol-1,4,5-trisphosphate phosphatase EC:3.1.3.56, Sphingomyelinase EC:3.1.4.12 and Nocturnin.


Pssm-ID: 460902 [Multi-domain]  Cd Length: 183  Bit Score: 58.78  E-value: 2.17e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560   29 FNVQSFGDSKSADAKIMNSISRIVARC--DICLLQEVRDQKNKAIPRLLEAVNkigdndfqYVASERLGRTQTYQEQYVF 106
Cdd:pfam03372   3 WNVNGGNADAAGDDRKLDALAALIRAYdpDVVALQETDDDDASRLLLALLAYG--------GFLSYGGPGGGGGGGGVAI 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  107 FYRKSTAILKGQYQYPDTQTGDIDAFSREPFVVYFqapktlaKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWETENV 186
Cdd:pfam03372  75 LSRYPLSSVILVDLGEFGDPALRGAIAPFAGVLVV-------PLVLTLAPHASPRLARDEQRADLLLLLLALLAPRSEPV 147


                  ....*...
gi 225690560  187 MFLGDFNA 194
Cdd:pfam03372 148 ILAGDFNA 155
COG2374 COG2374
Predicted extracellular nuclease [General function prediction only];
24-194 1.06e-04

Predicted extracellular nuclease [General function prediction only];


Pssm-ID: 441941 [Multi-domain]  Cd Length: 362  Bit Score: 43.47  E-value: 1.06e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  24 FRICAFNVQSFGDSKSAD-----------------AKIMNSISRIVArcDICLLQEVRDQkNKAIPRLLEAVN-KIGDND 85
Cdd:COG2374   69 LRVATFNVENLFDTDDDDddfgrgadtpeeyerklAKIAAAIAALDA--DIVGLQEVENN-GSALQDLVAALNlAGGTYA 145

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  86 FQYVASERLGRTQTyqeqyV-FFYRKSTAILKGQYQYPDT--QTGDIDAFSREPFVVYFQAPKTlaKEFVLIPQH----- 157
Cdd:COG2374  146 FVHPPDGPDGDGIR-----VaLLYRPDRVTLVGSATIADLpdSPGNPDRFSRPPLAVTFELANG--EPFTVIVNHfkskg 218

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 225690560 158 -------TTPSNA--TKEIDELYDvFVD-VKVRWETENVMFLGDFNA 194
Cdd:COG2374  219 sddpgdgQGASEAkrTAQAEALRA-FVDsLLAADPDAPVIVLGDFND 264
 
Name Accession Description Interval E-value
DNase1 cd10282
Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a ...
 25-283 3.05e-152

Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib, and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma) . DNASE1L3 is implicated in apoptotic DNA fragmentation. DNase I is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197337 [Multi-domain]  Cd Length: 256  Bit Score: 427.04  E-value: 3.05e-152
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  25 RICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDNDFQYVASERLGRTqTYQEQY 104
Cdd:cd10282    1 RIAAFNIQVFGESKMSKPEVMDVLVKILSRYDIVLIQEIRDSSGTAIPELLDELNSASSNTYSYVVSERLGRS-SYKEQY 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 105 VFFYRKSTAILKGQYQYPDTQTGDiDAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWETE 184
Cdd:cd10282   80 AFIYRSDKVSVLESYQYDDGDEGT-DVFSREPFVVRFSSPSTAVKDFVLVPIHTSPDDAVAEIDALYDVYDDVKQRWRED 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 185 NVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTVKeSTDCAYDRIVVHGEPFLRSIKPFSAQPFNFPKEFL 264
Cdd:cd10282  159 DVILLGDFNADCSYVTSKGWKSIRLRTDSRFHWLIGDDADTTVR-STNCAYDRIVVAGSLLQSAVVPGSAGVFDFDKEFG 237

                        250
                 ....*....|....*....
gi 225690560 265 LTEAQALEVSDHYPIEVVL 283
Cdd:cd10282  238 LTEEEALAVSDHYPVEVEL 256
DNaseIc smart00476
deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of ...
 5-284 6.64e-137

deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of double-stranded DNA. The enzyme is secreted outside the cell and also involved in apoptosis in the nucleus.


Pssm-ID: 128752  Cd Length: 276  Bit Score: 388.72  E-value: 6.64e-137
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560     5 IRRILLSIFLLSGLLTcygFRICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDN 84
Cdd:smart00476   2 VPSLLLFLLLLHGAAS---LRICAFNIQSFGDSKMSNATLMSIIVKILSRYDIALVQEVRDSDLSAVPKLMDQLNSDSPN 78

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560    85 DFQYVASERLGRTqTYQEQYVFFYRKSTAILKGQYQYPDTQTGDIDAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNAT 164
Cdd:smart00476  79 TYSYVSSEPLGRN-SYKEQYLFLYRSDLVSVLDSYLYDDGCECGNDVFSREPFVVKFSSPSTAVKEFVIVPLHTTPEAAV 157

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560   165 KEIDELYDVFVDVKVRWETENVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTVKeSTDCAYDRIVVHGEP 244
Cdd:smart00476 158 AEIDALYDVYLDVRQKWGTEDVIFMGDFNAGCSYVTKKQWSSIRLRTSPTFHWLIPDSADTTVT-STHCAYDRIVVAGER 236

                          250       260       270       280
                   ....*....|....*....|....*....|....*....|
gi 225690560   245 FLRSIKPFSAQPFNFPKEFLLTEAQALEVSDHYPIEVVLK 284
Cdd:smart00476 237 LRSSVVPGSAAVFDFQTAYGLTEEEALAISDHFPVEVTLK 276
DNase1-like cd09075
Deoxyribonuclease 1 and related proteins; This family includes Deoxyribonuclease 1 (DNase1, EC ...
 25-283 5.33e-66

Deoxyribonuclease 1 and related proteins; This family includes Deoxyribonuclease 1 (DNase1, EC 3.1.21.1) and related proteins. DNase1, also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma). DNASE1L3 is also implicated in apoptotic DNA fragmentation. DNase1 is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This family also includes a subfamily of mostly uncharacterized proteins, which includes Mycoplasma pulmonis MnuA, a membrane-associated nuclease. The in vivo role of MnuA is as yet undetermined. This family belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197309 [Multi-domain]  Cd Length: 258  Bit Score: 208.02  E-value: 5.33e-66
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  25 RICAFNVQSFGDSKSADAKIMNSISRIVARCDICLLQEVRDQKNKAIPRLLEAVNKIGDNDFQYVASERLGRTqTYQEQY 104
Cdd:cd09075    1 KIAAFNIRTFGETKMSNATLASYIVRIVRRYDIVLIQEVRDSHLVAVGKLLDYLNQDDPNTYHYVVSEPLGRN-SYKERY 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 105 VFFYRKSTAILKGQYQYpDTQTGDI--DAFSREPFVVYFQAPKTLAKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWE 182
Cdd:cd09075   80 LFLFRPNKVSVLDTYQY-DDGCKSCgnDSFSREPAVVKFSSHSTKVKEFAIVALHSAPSDAVAEINSLYDVYLDVQQKWH 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 183 TENVMFLGDFNAACGYVAKKNRKNIRLYTDPSFTWLIQDNVDTTvKESTDCAYDRIVVHGEPFLRSIKPFSAQPFNFPKE 262
Cdd:cd09075  159 LNDVMLMGDFNADCSYVTSSQWSSIRLRTSSTFQWLIPDSADTT-ATSTNCAYDRIVVAGSLLQSSVVPGSAAPFDFQAA 237

                        250       260
                 ....*....|....*....|.
gi 225690560 263 FLLTEAQALEVSDHYPIEVVL 283
Cdd:cd09075  238 YGLSNEMALAISDHYPVEVTL 258
MnuA_DNase1-like cd10283
Mycoplasma pulmonis MnuA nuclease-like; This subfamily includes Mycoplasma pulmonis MnuA, a ...
 24-283 1.85e-27

Mycoplasma pulmonis MnuA nuclease-like; This subfamily includes Mycoplasma pulmonis MnuA, a membrane-associated nuclease related to Deoxyribonuclease 1 (DNase1 or DNase I, EC 3.1.21.1). The in vivo role of MnuA is as yet undetermined. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197338 [Multi-domain]  Cd Length: 266  Bit Score: 107.87  E-value: 1.85e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  24 FRICAFNVQSFGDSKSADAKimNSISRIVAR--CDICLLQEVRD--QKNKAIPRLLEAVNKIGdNDFQYVASERLGRTQT 99
Cdd:cd10283    1 LRIASWNILNFGNSKGKEKN--PAIAEIISAfdLDLIALQEVMDngGGLDALAKLVNELNKPG-GTWKYIVSDKTGGSSG 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 100 YQEQYVFFYRKSTAILKGQ-YQYPDTQTgdiDAFSREPFVVYFQApKTLAKEFVLIPQH-------TTPSNA--TKEIDE 169
Cdd:cd10283   78 DKERYAFLYKSSKVRKVGKaVLEKDSNT---DGFARPPYAAKFKS-GGTGFDFTLVNVHlksggssKSGQGAkrVAEAQA 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 170 LYDVFVDVKVRWETENVMFLGDFNaacgyvAKKNRKNIRLYTDPSFTWLIQDnvDTTVKESTDC---AYDRIVVHGEPFL 246
Cdd:cd10283  154 LAEYLKELADEDPDDDVILLGDFN------IPADEDAFKALTKAGFKSLLPD--STNLSTSFKGyanSYDNIFVSGNLKE 225

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|.
gi 225690560 247 ----RSIKPFSAQPFNFPKEFLLTEAQALEVSDHYPIEVVL 283
Cdd:cd10283  226 kfsnSGVFDFNILVDEAGEEDLDYSKWRKQISDHDPVWVEF 266
EEP cd08372
Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes ...
 26-283 5.21e-16

Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes the catalytic domain (exonuclease/endonuclease/phosphatase or EEP domain) of a diverse set of proteins including the ExoIII family of apurinic/apyrimidinic (AP) endonucleases, inositol polyphosphate 5-phosphatases (INPP5), neutral sphingomyelinases (nSMases), deadenylases (such as the vertebrate circadian-clock regulated nocturnin), bacterial cytolethal distending toxin B (CdtB), deoxyribonuclease 1 (DNase1), the endonuclease domain of the non-LTR retrotransposon LINE-1, and related domains. These diverse enzymes share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins.


Pssm-ID: 197306 [Multi-domain]  Cd Length: 241  Bit Score: 75.98  E-value: 5.21e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  26 ICAFNVQSFGDSKSADAkimnsISRIVARC--DICLLQEVRDQKNKAIPRLLEAVNkiGDNDFQYVASERLGrtqtYQEQ 103
Cdd:cd08372    1 VASYNVNGLNAATRASG-----IARWVRELdpDIVCLQEVKDSQYSAVALNQLLPE--GYHQYQSGPSRKEG----YEGV 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 104 YVFFYRKSTAILKGqyqyPDTQTGDIDAFSREPFVVYFqapKTLAKEFVLIPQH-----TTPSNATKEIDELYDVFVDVK 178
Cdd:cd08372   70 AILSKTPKFKIVEK----HQYKFGEGDSGERRAVVVKF---DVHDKELCVVNAHlqaggTRADVRDAQLKEVLEFLKRLR 142

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560 179 vRWETENVMFLGDFNAACGYVAKKNRKN-IRLYTDPSFTWLIQDNVDT----TVKESTDCAYDRIVVHGEpFLRSIKpfS 253
Cdd:cd08372  143 -QPNSAPVVICGDFNVRPSEVDSENPSSmLRLFVALNLVDSFETLPHAytfdTYMHNVKSRLDYIFVSKS-LLPSVK--S 218

                        250       260       270
                 ....*....|....*....|....*....|
gi 225690560 254 AQPFNFPKEfllteaqALEVSDHYPIEVVL 283
Cdd:cd08372  219 SKILSDAAR-------ARIPSDHYPIEVTL 241
Exo_endo_phos pfam03372
Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium ...
 29-194 2.17e-10

Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signalling. This family includes: AP endonuclease proteins EC:4.2.99.18, DNase I proteins EC:3.1.21.1, Synaptojanin an inositol-1,4,5-trisphosphate phosphatase EC:3.1.3.56, Sphingomyelinase EC:3.1.4.12 and Nocturnin.


Pssm-ID: 460902 [Multi-domain]  Cd Length: 183  Bit Score: 58.78  E-value: 2.17e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560   29 FNVQSFGDSKSADAKIMNSISRIVARC--DICLLQEVRDQKNKAIPRLLEAVNkigdndfqYVASERLGRTQTYQEQYVF 106
Cdd:pfam03372   3 WNVNGGNADAAGDDRKLDALAALIRAYdpDVVALQETDDDDASRLLLALLAYG--------GFLSYGGPGGGGGGGGVAI 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  107 FYRKSTAILKGQYQYPDTQTGDIDAFSREPFVVYFqapktlaKEFVLIPQHTTPSNATKEIDELYDVFVDVKVRWETENV 186
Cdd:pfam03372  75 LSRYPLSSVILVDLGEFGDPALRGAIAPFAGVLVV-------PLVLTLAPHASPRLARDEQRADLLLLLLALLAPRSEPV 147


                  ....*...
gi 225690560  187 MFLGDFNA 194
Cdd:pfam03372 148 ILAGDFNA 155
COG2374 COG2374
Predicted extracellular nuclease [General function prediction only];
24-194 1.06e-04

Predicted extracellular nuclease [General function prediction only];


Pssm-ID: 441941 [Multi-domain]  Cd Length: 362  Bit Score: 43.47  E-value: 1.06e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  24 FRICAFNVQSFGDSKSAD-----------------AKIMNSISRIVArcDICLLQEVRDQkNKAIPRLLEAVN-KIGDND 85
Cdd:COG2374   69 LRVATFNVENLFDTDDDDddfgrgadtpeeyerklAKIAAAIAALDA--DIVGLQEVENN-GSALQDLVAALNlAGGTYA 145

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 225690560  86 FQYVASERLGRTQTyqeqyV-FFYRKSTAILKGQYQYPDT--QTGDIDAFSREPFVVYFQAPKTlaKEFVLIPQH----- 157
Cdd:COG2374  146 FVHPPDGPDGDGIR-----VaLLYRPDRVTLVGSATIADLpdSPGNPDRFSRPPLAVTFELANG--EPFTVIVNHfkskg 218

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 225690560 158 -------TTPSNA--TKEIDELYDvFVD-VKVRWETENVMFLGDFNA 194
Cdd:COG2374  219 sddpgdgQGASEAkrTAQAEALRA-FVDsLLAADPDAPVIVLGDFND 264
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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