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Conserved domains on  [gi|446746026|ref|WP_000823282|]
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MULTISPECIES: PTS galactitol transporter subunit IIB [Enterobacteriaceae]

Protein Classification

PTS sugar transporter subunit IIB; PTS fructose transporter subunit IIB( domain architecture ID 10793372)

PTS sugar transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of incoming substrates (such as ascorbate, lactose, galactitol) concomitantly with their translocation across the cell membrane| PTS fructose transporter subunit IIB is part of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system that catalyzes the phosphorylation of fructose concomitantly with its translocation across the cell membrane

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
1-94 3.43e-60

PTS galactitol transporter subunit IIB;


:

Pssm-ID: 182372  Cd Length: 94  Bit Score: 178.52  E-value: 3.43e-60
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVG 80
Cdd:PRK10310  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVG 80

                        90
                ....*....|....
gi 446746026 81 IEALQNKILTILQG 94
Cdd:PRK10310 81 IEALQNKILTILQG 94
 
Name Accession Description Interval E-value
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
1-94 3.43e-60

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 178.52  E-value: 3.43e-60
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVG 80
Cdd:PRK10310  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVG 80

                        90
                ....*....|....
gi 446746026 81 IEALQNKILTILQG 94
Cdd:PRK10310 81 IEALQNKILTILQG 94
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 4-92 2.41e-33

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 110.32  E-value: 2.41e-33
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  4 KIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFgDIPLVHGMPFVSGVGIEA 83
Cdd:cd05566   2 KILVACGTGVATSTVVASKVKELLKENGIDVKVEQCKIAEVPSLLDDADLIVSTTKVPEDY-GIPVINGLPFLTGIGEDK 80


                ....*....
gi 446746026 84 LQNKILTIL 92
Cdd:cd05566  81 VYEEILEAL 89
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 2-94 3.06e-24

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 87.53  E-value: 3.06e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  2 KRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFgDIPLVHGMPFVSGVGI 81
Cdd:COG3414   1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADDADLIVTTTDLAEEL-GIPVINVIPLLNGIDK 79

                        90
                ....*....|...
gi 446746026 82 EALQNKILTILQG 94
Cdd:COG3414  80 DEIKEKLLEALKE 92
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 4-87 7.96e-06

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 40.39  E-value: 7.96e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026   4 KIIVACGGAVATSTMAAEEIKELCQSHN-IPVELIQC-RVNEIETYMDGVHLICTTAKVD----RSFGDIPL--VHGMPF 75
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGiVEAQGAAGvNELTAEDIADDADVVILAPDVAvedlARFAGKPVyvIPVKDA 80

                         90
                 ....*....|..
gi 446746026  76 VSGVGIEALQNK 87
Cdd:pfam02302 81 LGMKDAEEVLEK 92
 
Name Accession Description Interval E-value
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
1-94 3.43e-60

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 178.52  E-value: 3.43e-60
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVG 80
Cdd:PRK10310  1 MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVG 80

                        90
                ....*....|....
gi 446746026 81 IEALQNKILTILQG 94
Cdd:PRK10310 81 IEALQNKILTILQG 94
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 4-92 2.41e-33

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 110.32  E-value: 2.41e-33
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  4 KIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFgDIPLVHGMPFVSGVGIEA 83
Cdd:cd05566   2 KILVACGTGVATSTVVASKVKELLKENGIDVKVEQCKIAEVPSLLDDADLIVSTTKVPEDY-GIPVINGLPFLTGIGEDK 80


                ....*....
gi 446746026 84 LQNKILTIL 92
Cdd:cd05566  81 VYEEILEAL 89
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 2-94 3.06e-24

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 87.53  E-value: 3.06e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  2 KRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFgDIPLVHGMPFVSGVGI 81
Cdd:COG3414   1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGIDAEVEHCDVSSAKSKADDADLIVTTTDLAEEL-GIPVINVIPLLNGIDK 79

                        90
                ....*....|...
gi 446746026 82 EALQNKILTILQG 94
Cdd:COG3414  80 DEIKEKLLEALKE 92
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 4-88 4.99e-12

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 56.11  E-value: 4.99e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026  4 KIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIEtYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEA 83
Cdd:cd00133   1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEAQGLSEVI-DLADADLIISTVPLAARFLGKPVIVVSPLLNEKDGEK 79


                ....*
gi 446746026 84 LQNKI 88
Cdd:cd00133  80 ILEKL 84
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 4-87 7.96e-06

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 40.39  E-value: 7.96e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446746026   4 KIIVACGGAVATSTMAAEEIKELCQSHN-IPVELIQC-RVNEIETYMDGVHLICTTAKVD----RSFGDIPL--VHGMPF 75
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGiVEAQGAAGvNELTAEDIADDADVVILAPDVAvedlARFAGKPVyvIPVKDA 80

                         90
                 ....*....|..
gi 446746026  76 VSGVGIEALQNK 87
Cdd:pfam02302 81 LGMKDAEEVLEK 92
PTS_IIB_mannitol cd05567
PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate: ...
 3-44 2.44e-04

PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a mannitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIA, IIB, and IIC domains are expressed from the mtlA gene as a single protein, also known as the mannitol PTS permease, the mtl transporter, or MtlA. MtlA is only functional as a dimer with the dimer contacts occuring between the IIC domains. MtlA takes up exogenous mannitol releasing the phosphate ester into the cytoplasm in preparation for oxidation to fructose-6-phosphate by the NAD-dependent mannitol-P dehydrogenase (MtlD). The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include mannitol, chitobiose/lichenan, ascorbate, lactose, galactitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99909 [Multi-domain]  Cd Length: 87  Bit Score: 36.40  E-value: 2.44e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 446746026  3 RKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI 44
Cdd:cd05567   1 KKIVFACDAGMGSSAMGASVLRKKLKKAGLEIPVTNSAIDEL 42
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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