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Conserved domains on  [gi|446992918|ref|WP_001070174|]
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MULTISPECIES: tRNA (guanosine(18)-2'-O)-methyltransferase TrmH [Enterobacteriaceae]

Protein Classification

tRNA (guanosine(18)-2'-O)-methyltransferase TrmH( domain architecture ID 11485189)

tRNA (guanosine(18)-2'-O)-methyltransferase TrmH is a member of SpoU family of MTases, which catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the conserved guanosine 18 (G18)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 1-228 0e+00

tRNA guanosine-2'-O-methyltransferase; Provisional


:

Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 500.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   1 MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG 80
Cdd:PRK11081   1 MNPERYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMGSTAAGSNSWVQVKTHRTIG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  81 DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:PRK11081  81 DAVAHLKGQGMQILATHLSDTAVDFREIDYTRPTCILMGQEKTGISQEALALADQDIIIPMIGMVQSLNVSVASALILYE 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446992918 161 AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAA 228
Cdd:PRK11081 161 AQRQRQNAGMYLRENSMLPEEEQQRLLFEGGYPVLAKVAKRKGLPYPHIDEQGQIVADADWWATMQAA 228
 
Name Accession Description Interval E-value
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 1-228 0e+00

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 500.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   1 MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG 80
Cdd:PRK11081   1 MNPERYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMGSTAAGSNSWVQVKTHRTIG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  81 DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:PRK11081  81 DAVAHLKGQGMQILATHLSDTAVDFREIDYTRPTCILMGQEKTGISQEALALADQDIIIPMIGMVQSLNVSVASALILYE 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446992918 161 AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAA 228
Cdd:PRK11081 161 AQRQRQNAGMYLRENSMLPEEEQQRLLFEGGYPVLAKVAKRKGLPYPHIDEQGQIVADADWWATMQAA 228
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 5-165 1.45e-90

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 263.56  E-value: 1.45e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   5 RYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVA 84
Cdd:cd18092    2 RLERIEEVLARRTRDLTVVLENVHDPHNASAVLRTCDAFGVQDVHVIEPKNKFKKNRGVAMGADKWLDVHRHDSTEECLA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  85 HLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
Cdd:cd18092   82 ALKEQGYRIVATSLSPDAVPLDELDYTRPTALVFGNEKDGVSEEALAAADERVYIPMYGFVESLNVSVAAAIILYELQRQ 161


                 .
gi 446992918 165 R 165
Cdd:cd18092  162 R 162
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 19-159 3.15e-49

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 157.69  E-value: 3.15e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   19 DLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGS---RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILA 95
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSadpYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918   96 THLsdNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILY 159
Cdd:pfam00588  81 TSL--GGKSLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 1-168 9.50e-42

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 142.64  E-value: 9.50e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   1 MNPTRYARICEMLA-RRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVhaVWPGSRM----RTMASAAAGSNSWVQVKT 75
Cdd:COG0566   96 VRPPEYADLEDLLAeAGEPPLLLVLDGVQDPGNLGAILRSAAAFGVDGV--ILPKRRAdpynPKVVRASAGALFHVPVAR 173

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  76 HRTIGDAVAHLKGQGMQILATHLsDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASA 155
Cdd:COG0566  174 VTNLADALEELKEAGFWVVGTDL-DGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDELVRIPMAGGVESLNVAVAAA 252

                        170
                 ....*....|...
gi 446992918 156 LILYEAQRQRQNA 168
Cdd:COG0566  253 ILLYEAARQRRAK 265
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 30-165 2.90e-19

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 83.16  E-value: 2.90e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   30 PHNVSAIIRTADAVGVHEVhaVWPGSRM----RTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDF 105
Cdd:TIGR00186 101 PHNLGAILRTAEAFGVDGV--ILPKRRSaplnSTVVKTSSGAVEYVPLARVTNLSRTITKLKESGFWTVGTDLDAQDTLY 178

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  106 REIDyTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQR 165
Cdd:TIGR00186 179 QVKL-TKPLALVVGNEGEGVSRLIKENCDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQR 237
 
Name Accession Description Interval E-value
PRK11081 PRK11081
tRNA guanosine-2'-O-methyltransferase; Provisional
 1-228 0e+00

tRNA guanosine-2'-O-methyltransferase; Provisional


Pssm-ID: 236837 [Multi-domain]  Cd Length: 229  Bit Score: 500.29  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   1 MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG 80
Cdd:PRK11081   1 MNPERYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMGSTAAGSNSWVQVKTHRTIG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  81 DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:PRK11081  81 DAVAHLKGQGMQILATHLSDTAVDFREIDYTRPTCILMGQEKTGISQEALALADQDIIIPMIGMVQSLNVSVASALILYE 160

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446992918 161 AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAA 228
Cdd:PRK11081 161 AQRQRQNAGMYLRENSMLPEEEQQRLLFEGGYPVLAKVAKRKGLPYPHIDEQGQIVADADWWATMQAA 228
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 5-165 1.45e-90

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 263.56  E-value: 1.45e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   5 RYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVA 84
Cdd:cd18092    2 RLERIEEVLARRTRDLTVVLENVHDPHNASAVLRTCDAFGVQDVHVIEPKNKFKKNRGVAMGADKWLDVHRHDSTEECLA 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  85 HLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
Cdd:cd18092   82 ALKEQGYRIVATSLSPDAVPLDELDYTRPTALVFGNEKDGVSEEALAAADERVYIPMYGFVESLNVSVAAAIILYELQRQ 161


                 .
gi 446992918 165 R 165
Cdd:cd18092  162 R 162
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 19-159 3.15e-49

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 157.69  E-value: 3.15e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   19 DLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGS---RMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILA 95
Cdd:pfam00588   1 PLVVVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSadpYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVA 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918   96 THLsdNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILY 159
Cdd:pfam00588  81 TSL--GGKSLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRIPMSGDVESLNVAVAAAIALY 142
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 1-168 9.50e-42

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 142.64  E-value: 9.50e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   1 MNPTRYARICEMLA-RRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVhaVWPGSRM----RTMASAAAGSNSWVQVKT 75
Cdd:COG0566   96 VRPPEYADLEDLLAeAGEPPLLLVLDGVQDPGNLGAILRSAAAFGVDGV--ILPKRRAdpynPKVVRASAGALFHVPVAR 173

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  76 HRTIGDAVAHLKGQGMQILATHLsDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASA 155
Cdd:COG0566  174 VTNLADALEELKEAGFWVVGTDL-DGEASLYEVDLPGPVALVLGNEGKGLSRLVLEACDELVRIPMAGGVESLNVAVAAA 252

                        170
                 ....*....|...
gi 446992918 156 LILYEAQRQRQNA 168
Cdd:COG0566  253 ILLYEAARQRRAK 265
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 20-161 1.74e-35

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 122.67  E-value: 1.74e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  20 LTVCMEQVHKPHNVSAIIRTADAVGVHEVHA--VWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATH 97
Cdd:cd18097    1 LVVVLDNLRSAHNVGSIFRTADAAGVEKIYLcgYTPTPPHPKVAKTALGAEESVPWEHFEDTLEAVRALKARGYTIIALE 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918  98 LSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEA 161
Cdd:cd18097   81 TTENSVPLHEARLPRPLALVLGNEVRGVSPEVLALCDAVVEIPMFGRKNSLNVAVAAAVALYEL 144
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 30-161 1.66e-34

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 120.22  E-value: 1.66e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  30 PHNVSAIIRTADAVGVHEV----HAVWP--GSRMRtmasAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLsDNAV 103
Cdd:cd18095   11 PGNLGTIIRTAAAFGVDGVilskGTVDPynPKVVR----ASMGALFRLPIIRVDDLEELLEELKEAGYKVVAADL-DGGS 85

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 446992918 104 DFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEA 161
Cdd:cd18095   86 SLYDLDLPKPVALVLGNEGNGLSDELLELCDKRVTIPMSGGVESLNVAVAAAILLYEL 143
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 25-165 9.31e-32

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 113.06  E-value: 9.31e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  25 EQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRT--MASAAAGSNSWVQVkTHRTIGDAVAHLKGQGMQILATHLsDNA 102
Cdd:cd18104    6 EGLEKPGNLGAILRSADAAGADAVILCDPKTDLFNpnVIRASLGAVFTLPV-AEASSEETLAWLKENKIRILATTP-KAE 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446992918 103 VDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQR 165
Cdd:cd18104   84 KLYYEADLRGPIAIVVGSESEGLSEFWLKAADERVRIPMAGQADSLNVSVAAAILLYEALRQR 146
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 22-161 1.54e-31

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 112.48  E-value: 1.54e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  22 VCMEQVHKPHNVSAIIRTADAVGVHEV-----HAVWPGSrmrTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILAT 96
Cdd:cd18103    3 LILDGITDPHNLGAIIRSAEAFGVDGViipkrRSAPLTP---TVAKASAGALEHLPIARVTNLARALEELKEAGFWIYGA 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446992918  97 HLsDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEA 161
Cdd:cd18103   80 DG-EAEKSLYDVDLTGPLALVLGSEGKGLRRLVLKNCDFLVKIPMAGKVDSLNVSVAAGIILYEI 143
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 21-161 8.76e-31

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 110.78  E-value: 8.76e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  21 TVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRM--RTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHL 98
Cdd:cd18082    1 HLVLDNIRSPGNIGSIFRTADAFGVEKIYLVGPTADIfdPKAKRTSRGTHFSVPYERVDNFEEYLKSLKEEGYPLIGLEL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446992918  99 SDNAVDFREIDY--TRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEA 161
Cdd:cd18082   81 TDGSIPLSEFKFkkPKKIALIFGNERSGLSPELLALCDYTVHIPMSGKNSSLNVSVATGIALYEF 145
SpoU-like cd18096
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 22-160 2.53e-30

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349969  Cd Length: 140  Bit Score: 109.23  E-value: 2.53e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  22 VCMEQVHKPHNVSAIIRTADAVGVHEVHAVwpGSRmRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDN 101
Cdd:cd18096    3 LVLHNVAKRHNIGTIVRSANAFGVKEVHLV--GRR-KFNPFGAMGTDRHVKFRHFDTLAEAKEYLKERGIRLVGVEIVPG 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918 102 AVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIG-MVQSLNVSVASALILYE 160
Cdd:cd18096   80 AEPIETHPFPGDTAFVLGNEGTGLSDEQLAICDGFVYIPQYGsGTASLNVSVAASIVLHH 139
SpoU_methylas_C pfam12105
SpoU, rRNA methylase, C-terminal; This domain is found in bacteria. This domain is about 60 ...
 163-218 4.09e-27

SpoU, rRNA methylase, C-terminal; This domain is found in bacteria. This domain is about 60 amino acids in length. This domain is found in association with pfam00588. This domain has a conserved LFE sequence motif. Some members of the Pfam family SpoU_methylase, pfam00588, carry this very distinctive sequence region at their extreme C-terminus. The exact function of this region is not known.


Pssm-ID: 463460  Cd Length: 54  Bit Score: 98.39  E-value: 4.09e-27
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 446992918  163 RQRQNAGMYlrENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD 218
Cdd:pfam12105   1 RQRQAAGMY--DRPRLSEEEYQRLLFEWGYPVLAKLCRRKGLPYPALDEDGEILAD 54
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 20-160 3.26e-22

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 88.36  E-value: 3.26e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  20 LTVCMEQVHKPHNVSAIIRTADAVGVHEVHAvwPGSRMRTMA---SAAAGSNSWVQVKTHR--TIGDAVAHLKGQGMQIL 94
Cdd:cd18091    1 LIVVASLIDKPPNLGGLCRTCEIFGVSKLVL--PNLAVVEDKqfkALSVSAEKWLPIEEVKpeDLLPYLKEKKQEGYTIV 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446992918  95 ATHLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:cd18091   79 GLEQTANSVSLHDFQFPKKTVLVLGNEKEGIPAELLSLLDVCVEIPQLGVIRSLNVHVSGAIAIYE 144
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 30-165 2.90e-19

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 83.16  E-value: 2.90e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918   30 PHNVSAIIRTADAVGVHEVhaVWPGSRM----RTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDF 105
Cdd:TIGR00186 101 PHNLGAILRTAEAFGVDGV--ILPKRRSaplnSTVVKTSSGAVEYVPLARVTNLSRTITKLKESGFWTVGTDLDAQDTLY 178

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  106 REIDyTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQR 165
Cdd:TIGR00186 179 QVKL-TKPLALVVGNEGEGVSRLIKENCDFLIKIPMAGKVDSLNVSVAAGILLFEIKRQR 237
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 29-161 2.38e-17

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 75.89  E-value: 2.38e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  29 KPHNVSAIIRTADAVGVHEV----HA--VWPGSRMRtmasAAAGS---NSWVQVKTHRTIGDAVAHLK--GQGMQILATH 97
Cdd:cd18107   10 SPGNLGTIIRSADALGAHGLvvtgHAadVYDPKTIR----ASTGSlfsLPIVRVPSNRELEDWVEARKarGTPLQLVGTD 85

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918  98 LSDNaVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEA 161
Cdd:cd18107   86 EHGD-HQIADHDFTRPTLLLIGNETAGLSAAWRELCDETVSIPMAGSASSLNAACAASIFLYEA 148
PRK11181 PRK11181
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;
11-165 3.93e-17

23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;


Pssm-ID: 183021 [Multi-domain]  Cd Length: 244  Bit Score: 77.31  E-value: 3.93e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  11 EMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVhaVWPGSR-------MRTMASAAAGSNSWVQVKT-HRTIgda 82
Cdd:PRK11181  87 DLLASLEQPFLLILDGVTDPHNLGACLRSADAAGVHAV--IVPKDRsaqlnatAKKVACGAAETVPLIRVTNlARTM--- 161

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  83 vAHLKGQGMQIL-----ATHlsdnavDFREIDYTRPTCILMGQEKTG---ITEEAlalADRDIIIPMIGMVQSLNVSVAS 154
Cdd:PRK11181 162 -RMLQEKNIWIVgtageADH------TLYQSKLTGPLALVMGAEGEGmrrLTREH---CDELISIPMAGSVSSLNVSVAT 231

                        170
                 ....*....|.
gi 446992918 155 ALILYEAQRQR 165
Cdd:PRK11181 232 GICLFEAVRQR 242
SpoU-like_TrmL cd18094
SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL ...
 30-164 7.81e-17

SAM-dependent tRNA methylase related to TrmL; tRNA (Um34/Cm34) methyltransferase TrmL catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 34 in both tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349967  Cd Length: 145  Bit Score: 74.41  E-value: 7.81e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  30 PHNVSAIIRTADAVGVhEVHAVWP-G-----SRMRTmasaaAGSNSW--VQVKTHRTIGDAVAHLKGQGMQILATHLSDN 101
Cdd:cd18094   10 PQNTGNIARTCAATGA-RLHLIEPlGfslddKRLKR-----AGLDYWdhVDLTVHDSWEAFLEALPPGRLFLFTTKGTKP 83

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918 102 AVDFreiDYTRPTCILMGQEKTGITEEAL-ALADRDIIIPMIGMVQSLNVSVASALILYEAQRQ 164
Cdd:cd18094   84 YTDF---DFKPGDYLVFGRESTGLPEEILdAFPDRRLRIPMRPKVRSLNLSNSVAIVLYEALRQ 144
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 20-160 1.64e-14

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 68.31  E-value: 1.64e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  20 LTVCMEQVHKPHNVSAIIRTA-----DAVGVHEVHAVWPGSrmrTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQIL 94
Cdd:cd18105    1 LWLLLDEITDPQNLGAILRSAyflgvDGVVVSSKNSAPLSP---VVSKASAGALELMPIYSVDNLASFLKSSKENGWKVV 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  95 ATHLSDNA---------VDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMI-----GMVQSLNVSVASALILYE 160
Cdd:cd18105   78 GTSAPPSAssdkktipaNELSELLLEKPTILVLGNEGRGLRTNIKNLCDYLVSIPGGrsaenPGVDSLNVSVAAGILLHS 157
TrmL COG0219
tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ...
 30-170 4.32e-14

tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain [Translation, ribosomal structure and biogenesis]; tRNA(Leu) C34 or U34 (ribose-2'-O)-methylase TrmL, contains SPOUT domain is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439989  Cd Length: 153  Bit Score: 66.98  E-value: 4.32e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  30 PHNVSAIIRTADAVGVhEVHAVWP-G-----SRMRTmasaaAGSNSW--VQVKTHRTIGDAVAHLKGQGMqILAT----- 96
Cdd:COG0219   12 PQNTGNIIRLCAATGA-RLHLIEPlGfslddKKLKR-----AGLDYWelVDLTVHDSWEAFLEALPGGRL-FLFTtkgtk 84

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 446992918  97 HLSDnaVDFREIDYtrptcILMGQEKTGITEEALAL-ADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGM 170
Cdd:COG0219   85 PYTD--VDYQPGDV-----LLFGRETAGLPEEVLAApADQRLRIPMRPGVRSLNLSNAVAIVLYEALRQLGFPGL 152
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 20-160 4.81e-14

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 66.79  E-value: 4.81e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  20 LTVCMEQVHKPHNVSAIIRTADAVGVHEVHA------VW-PGSRMRTMASAAagsnswvQVKTHRTIGDAVAHLKGQGMQ 92
Cdd:cd18109    1 LTLALDGIQDPGNLGTIIRTADWFGIRQIICspdtvdPYnPKVVQASMGSIF-------RVQIHYTDLPALLAELKKAFP 73

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 446992918  93 ILATHLSDNAVdfREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIG-MVQSLNVSVASALILYE 160
Cdd:cd18109   74 VYGADLDGENL--YEFTLPEKGILVMGNEGHGISPEVLKLLTHRLTIPRYGkGAESLNVAVATAILLSE 140
PRK10864 PRK10864
putative methyltransferase; Provisional
 19-166 3.50e-13

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 67.50  E-value: 3.50e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  19 DLTVCMEQVHKPHNVSAIIRTADAVGV-----HEVHAVWPGSRMRTmasaAAGSNSWVQVKTHRTIGDAVAHLKGQGMQI 93
Cdd:PRK10864 198 DCVLALEDVGNPHNLGGIMRSCAHFGVkgvvvQDAALLESGAAIRT----AEGGAEHVQPITGDSFVDVLDDFRQAGYTI 273

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446992918  94 LATHlSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQ 166
Cdd:PRK10864 274 VTTS-SHKGTPLFKASLPAKMVLVLGQEYDGLSDAARQQGDLSVSIDGTGNVESLNVSVATGVLLAEWWRQNK 345
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 20-161 8.73e-13

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 63.42  E-value: 8.73e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  20 LTVCMEQVHKPHNVSAIIRTADAVGVHEVHA------VWPGSRMRtmasAAAGSN---------SWvqvkthrtigDAVA 84
Cdd:cd18106    1 LTVICDNIRDPGNLGSILRSAAAAGCSKVLLtkgcvdPWEPKVLR----AGAGAHfrlpiygnlSW----------DDIP 66

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  85 HLKGQGMQIlatHLSDN---AVDFREIDYTR-PTCILMGQEKTGITEEALALADRD----IIIPMIGMVQSLNVSVASAL 156
Cdd:cd18106   67 NYLPDDSKV---FLADNnlpVQPYDSIDYCSgPIVLVIGGETHGVSPEAYKLAHSTggsrVHIPLANGVDSLNSAVAASI 143


                 ....*
gi 446992918 157 ILYEA 161
Cdd:cd18106  144 LLFEM 148
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 32-160 6.41e-11

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 58.26  E-value: 6.41e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  32 NVSAIIRTADAVGVHEVhaVWPGSRMRTMAS-----AAAGSN-SWVQVKTHRTigDAVAHLKGQGMQIlATHLSDNAVDF 105
Cdd:cd18108   13 NIGAIVRTSLALGAAGI--VLVDSDLATVADrrllrASRGYVfSLPVVLADRE--EAVAFLRDNDIPL-MTLDADGDVPV 87

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 446992918 106 REI-DYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:cd18108   88 KELgDRPDRLALVFGSEKGGPSGLFEEAAAGTVSIPMSSSTESLNVSVSAGIALHE 143
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 30-160 2.29e-09

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 54.42  E-value: 2.29e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  30 PHNVSAIIRTADAVGVHEV-----HAVWPGSRMRTMASAAagSNSWVQVKTHRTIGDAVAHL--------KGQGMQILAT 96
Cdd:cd18093   11 PGNIGAVARAMKNFGLSDLrlvnpRCDDPEEAARALASGA--EDVLENAKVYDTLEEALADCdlvvattaRPRGLRRPLL 88

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918  97 HLSDNAVDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE 160
Cdd:cd18093   89 TPRELAELLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTIPTNPEYPSLNLAQAVAIVLYE 152
SpoU-like cd18098
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 22-159 4.22e-09

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349971  Cd Length: 138  Bit Score: 53.42  E-value: 4.22e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  22 VCMEQVHKPHNVSAIIRTADAVGVHEVHAVwpGSRMRTMASAAAGSNSWVQVKTHR--TIGDAVAHLKgQGMQILATHLS 99
Cdd:cd18098    3 IGIENPKTPKNVGTLWRAAGCLGASFVFTI--GARYKRRKQSTDTSKAWRHIPLFHydDLEDLAEPLP-FGAVLVAVELT 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 446992918 100 DNAVDFreIDYTRP--TCILMGQEKTGITEEALALADRDIIIPMIGmvqSLNVSVASALILY 159
Cdd:cd18098   80 EGATPL--PDFKHPdrAVYLLGPEDGGLPQEVLARCHHVVYLPTRG---SLNVAVAGSIVLY 136
SPOUT_MTase cd07060
SPOUT superfamily of SAM-dependent RNA methyltransferases; The SPOUT (SpoU-TrmD) ...
 21-161 4.48e-09

SPOUT superfamily of SAM-dependent RNA methyltransferases; The SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, also known as class IV methyltransferase family, is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot. Members of the SPOUT superfamily that have been characterized functionally are involved in post-transcriptional RNA modification by catalyzing methylation of the 2-OH group of ribose, the N-1 atom of guanosine 37 in tRNA, or the N-3 atom of uridine 1498 in 16S rRNA.


Pssm-ID: 349952  Cd Length: 99  Bit Score: 52.44  E-value: 4.48e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  21 TVCMEQVHKPHNVSAIIRTADAVGVHEVHAVwpgsrmrtmasaaagsnswvqvkthrtigdavahlkgqgmQILATHLSD 100
Cdd:cd07060    1 RIVIDEKKIPEKVDKIIRAALAEYAKRLSIY----------------------------------------GIVTLSSEG 40

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446992918 101 NAVDFRE--IDYTRPTCILMGQEKTGITEEALALADRDIIIPMigmvQSLNVSVASALILYEA 161
Cdd:cd07060   41 FAVKISEleISGGSNIAFVIGGEYGGLEESARALADEPISISS----MTLSAELAATILLEQL 99
PRK10358 PRK10358
tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase ...
 30-173 9.76e-07

tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL;


Pssm-ID: 182406  Cd Length: 157  Bit Score: 47.30  E-value: 9.76e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  30 PHNVSAIIRTADAVGVhEVHAV------WPGSRMRtmaSAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATH--LSDN 101
Cdd:PRK10358  12 PPNTGNIIRLCANTGF-RLHIIepmgfaWDDKRLR---RAGLDYHEFTAVTRHHDYAAFLEAENPQRLFALTTKgtPAHS 87

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446992918 102 AVDFREIDYtrptcILMGQEKTGITEEAL-AL-ADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLR 173
Cdd:PRK10358  88 AVSYQDGDY-----LMFGPETRGLPASILdALpAEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLR 156
rRNA_methyl_1 TIGR00050
RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of ...
 113-189 1.89e-05

RNA methyltransferase, TrmH family, group 1; This is part of the trmH (spoU) family of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases, and is now characterized, in E. coli, as a tRNA:Cm32/Um32 methyltransferase. It may be named TrMet(Xm32), or TrmJ, according to the nomenclature style chosen [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272876 [Multi-domain]  Cd Length: 233  Bit Score: 44.31  E-value: 1.89e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  113 PTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE---AQRQRQNAgmylRENSMLPEAEQQRLLFE 189
Cdd:TIGR00050 105 KVALVFGREDSGLTNEELQKCHVLVSIPTSEEYPSLNLSHAVAVILYElrmAWLAQQEN----LSEMPYATVDDLELLLE 180
TrmJ COG0565
tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ...
 51-208 3.66e-05

tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase [Translation, ribosomal structure and biogenesis]; tRNA C32,U32 (ribose-2'-O)-methylase TrmJ or a related methyltransferase is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440331 [Multi-domain]  Cd Length: 237  Bit Score: 43.57  E-value: 3.66e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918  51 VWPGSRMRTMASAAagsnSWV--QVKTHRTIGDAVAHL--------KGQGMQILATHLSDNAVDFREIDYTRPTCILMGQ 120
Cdd:COG0565   39 GWPDEEARAMASGA----DDVleNARVVDTLEEALADCdlvvgttaRPRELRKPVLTPREAAEELLARAAGGKVALVFGR 114

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446992918 121 EKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSM-LPEAEQQRLLFEggypVLAKVA 199
Cdd:COG0565  115 ERSGLTNEELALCDALVTIPTNPEYPSLNLAQAVQVVAYELRKAALEAPPLPPPPEPePATAEELEGLYE----HLEELL 190


                 ....*....
gi 446992918 200 KRKGLPYPH 208
Cdd:COG0565  191 DAIGFLDPE 199
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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