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Conserved domains on  [gi|495775328|ref|WP_008499907|]
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MULTISPECIES: winged helix-turn-helix domain-containing protein [Enterobacter]

Protein Classification

winged helix-turn-helix domain-containing protein( domain architecture ID 10007860)

winged helix-turn-helix (wHTH) domain-containing protein binds DNA and may function as a transcriptional regulator

CATH:  1.10.10.10
Gene Ontology:  GO:0003677
PubMed:  10473770|8831795|15808743
SCOP:  3000034

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
7-112 2.51e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


:

Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 106.14  E-value: 2.51e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   7 NSLLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEefGFTPSGNNLNKAVSELRKSFHALG 86
Cdd:COG3710    6 GDFRLDPARRELLRDGEP---VRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGDDG 80

                         90       100
                 ....*....|....*....|....*.
gi 495775328  87 DSHDYIVTVPRFGFRFDADVIFKPAE 112
Cdd:COG3710   81 LEPRLIRTVPRRGYRLVAPVEVEAPE 106
 
Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
7-112 2.51e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 106.14  E-value: 2.51e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   7 NSLLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEefGFTPSGNNLNKAVSELRKSFHALG 86
Cdd:COG3710    6 GDFRLDPARRELLRDGEP---VRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGDDG 80

                         90       100
                 ....*....|....*....|....*.
gi 495775328  87 DSHDYIVTVPRFGFRFDADVIFKPAE 112
Cdd:COG3710   81 LEPRLIRTVPRRGYRLVAPVEVEAPE 106
trans_reg_C cd00383
DNA-binding effector domain of two-component system response regulators; Bacteria and some ...
 9-102 2.82e-17

DNA-binding effector domain of two-component system response regulators; Bacteria and some eukaryotes use two-component signal transduction systems to detect and respond to changes in the environment. The systems consists of a sensor histidine kinase and a response regulator. The former autophosphorylates a histidine residue on detecting an external stimulus. The phosphate is then transferred to an invariant aspartate residue in a highly conserved receiver domain of the response regulator. Phosphorylation activates a variable effector domain of the response regulator, which triggers the cellular response. This C-terminal effector domain belongs to the winged helix-turn-helix family of transcriptional regulators and contains DNA and RNA polymerase binding sites. Several dimers or monomers bind head to tail to small tandem repeats upstream of the genes. The RNA polymerase binding sites interact with the alpha or sigma subunit of RNA polymerase.


Pssm-ID: 294013 [Multi-domain]  Cd Length: 89  Bit Score: 74.44  E-value: 2.82e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   9 LLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDs 88
Cdd:cd00383    1 LRLDPARREVTVDGEE---VELTPKEFELLELLARNPGRVVSREELLEAVWGY-DYEVDDRTVDVHISRLRKKLEDDPE- 75

                         90
                 ....*....|....
gi 495775328  89 HDYIVTVPRFGFRF 102
Cdd:cd00383   76 PRLIKTVRGVGYRL 89
Trans_reg_C smart00862
Transcriptional regulatory protein, C terminal; This domain is almost always found associated ...
 27-102 8.61e-15

Transcriptional regulatory protein, C terminal; This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding.


Pssm-ID: 214866 [Multi-domain]  Cd Length: 76  Bit Score: 67.19  E-value: 8.61e-15
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 495775328    27 VLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDsHDYIVTVPRFGFRF 102
Cdd:smart00862   3 PIKLTPKEFRLLELLLRNPGRVVSREELLEAVWGD-DEDVDDNTLDVHISRLRKKLEDDGD-PRLIETVRGVGYRL 76
Trans_reg_C pfam00486
Transcriptional regulatory protein, C terminal;
28-102 2.17e-14

Transcriptional regulatory protein, C terminal;


Pssm-ID: 425712 [Multi-domain]  Cd Length: 77  Bit Score: 66.38  E-value: 2.17e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 495775328   28 LTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDSHDYIVTVPRFGFRF 102
Cdd:pfam00486   4 VELTPKEFKLLELLAENPGRVVSREQLLEEVWGE-DYDVDDNTVDVHISRLRKKLEDDPRDPRLIKTVRGVGYRL 77
PRK10153 PRK10153
DNA-binding transcriptional activator CadC; Provisional
 27-168 8.31e-11

DNA-binding transcriptional activator CadC; Provisional


Pssm-ID: 236658 [Multi-domain]  Cd Length: 517  Bit Score: 61.60  E-value: 8.31e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328  27 VLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEefGFTPSGNNLNKAVSELRKSFHALG-DSHDYIVTVPRFGFRFDAD 105
Cdd:PRK10153  26 EVTLEPRLIDLLVFFAQHSGEVLSRDELIDHVWD--RAIVTDHVVTQSISELRKSLKDGReDNPEYIVTVPKRGYKLVAP 103

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328 106 VIF--------------KPAESVTPEVSQPS----------------VEQPSGYKRLSKKW-WLVLAV-LTTSV--VGFS 151
Cdd:PRK10153 104 VIWyseeegeeilasspPPIPEAVPATAEPSesanaqfpappltravCQSPAKSKRFTTFWvWRLFLLsLGICVllVAMS 183

                        170
                 ....*....|....*..
gi 495775328 152 LFFSRQLEITVPATLKP 168
Cdd:PRK10153 184 SLTYRQPVVKIRQLLNP 200
 
Name Accession Description Interval E-value
CadC1 COG3710
DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];
7-112 2.51e-29

DNA-binding winged helix-turn-helix (wHTH) domain [Transcription];


Pssm-ID: 442924 [Multi-domain]  Cd Length: 106  Bit Score: 106.14  E-value: 2.51e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   7 NSLLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEefGFTPSGNNLNKAVSELRKSFHALG 86
Cdd:COG3710    6 GDFRLDPARRELLRDGEP---VRLSPRAFDLLLLLLEHPGRVVSRDELLAAVWP--GRVVSDNSLTQAISRLRKALGDDG 80

                         90       100
                 ....*....|....*....|....*.
gi 495775328  87 DSHDYIVTVPRFGFRFDADVIFKPAE 112
Cdd:COG3710   81 LEPRLIRTVPRRGYRLVAPVEVEAPE 106
trans_reg_C cd00383
DNA-binding effector domain of two-component system response regulators; Bacteria and some ...
 9-102 2.82e-17

DNA-binding effector domain of two-component system response regulators; Bacteria and some eukaryotes use two-component signal transduction systems to detect and respond to changes in the environment. The systems consists of a sensor histidine kinase and a response regulator. The former autophosphorylates a histidine residue on detecting an external stimulus. The phosphate is then transferred to an invariant aspartate residue in a highly conserved receiver domain of the response regulator. Phosphorylation activates a variable effector domain of the response regulator, which triggers the cellular response. This C-terminal effector domain belongs to the winged helix-turn-helix family of transcriptional regulators and contains DNA and RNA polymerase binding sites. Several dimers or monomers bind head to tail to small tandem repeats upstream of the genes. The RNA polymerase binding sites interact with the alpha or sigma subunit of RNA polymerase.


Pssm-ID: 294013 [Multi-domain]  Cd Length: 89  Bit Score: 74.44  E-value: 2.82e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   9 LLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDs 88
Cdd:cd00383    1 LRLDPARREVTVDGEE---VELTPKEFELLELLARNPGRVVSREELLEAVWGY-DYEVDDRTVDVHISRLRKKLEDDPE- 75

                         90
                 ....*....|....
gi 495775328  89 HDYIVTVPRFGFRF 102
Cdd:cd00383   76 PRLIKTVRGVGYRL 89
Trans_reg_C smart00862
Transcriptional regulatory protein, C terminal; This domain is almost always found associated ...
 27-102 8.61e-15

Transcriptional regulatory protein, C terminal; This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding.


Pssm-ID: 214866 [Multi-domain]  Cd Length: 76  Bit Score: 67.19  E-value: 8.61e-15
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 495775328    27 VLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDsHDYIVTVPRFGFRF 102
Cdd:smart00862   3 PIKLTPKEFRLLELLLRNPGRVVSREELLEAVWGD-DEDVDDNTLDVHISRLRKKLEDDGD-PRLIETVRGVGYRL 76
Trans_reg_C pfam00486
Transcriptional regulatory protein, C terminal;
28-102 2.17e-14

Transcriptional regulatory protein, C terminal;


Pssm-ID: 425712 [Multi-domain]  Cd Length: 77  Bit Score: 66.38  E-value: 2.17e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 495775328   28 LTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEfGFTPSGNNLNKAVSELRKSFHALGDSHDYIVTVPRFGFRF 102
Cdd:pfam00486   4 VELTPKEFKLLELLAENPGRVVSREQLLEEVWGE-DYDVDDNTVDVHISRLRKKLEDDPRDPRLIKTVRGVGYRL 77
PRK10153 PRK10153
DNA-binding transcriptional activator CadC; Provisional
 27-168 8.31e-11

DNA-binding transcriptional activator CadC; Provisional


Pssm-ID: 236658 [Multi-domain]  Cd Length: 517  Bit Score: 61.60  E-value: 8.31e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328  27 VLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEefGFTPSGNNLNKAVSELRKSFHALG-DSHDYIVTVPRFGFRFDAD 105
Cdd:PRK10153  26 EVTLEPRLIDLLVFFAQHSGEVLSRDELIDHVWD--RAIVTDHVVTQSISELRKSLKDGReDNPEYIVTVPKRGYKLVAP 103

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328 106 VIF--------------KPAESVTPEVSQPS----------------VEQPSGYKRLSKKW-WLVLAV-LTTSV--VGFS 151
Cdd:PRK10153 104 VIWyseeegeeilasspPPIPEAVPATAEPSesanaqfpappltravCQSPAKSKRFTTFWvWRLFLLsLGICVllVAMS 183

                        170
                 ....*....|....*..
gi 495775328 152 LFFSRQLEITVPATLKP 168
Cdd:PRK10153 184 SLTYRQPVVKIRQLLNP 200
OmpR COG0745
DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain ...
 10-94 1.81e-08

DNA-binding response regulator, OmpR family, contains REC and winged-helix (wHTH) domain [Signal transduction mechanisms, Transcription];


Pssm-ID: 440508 [Multi-domain]  Cd Length: 204  Bit Score: 53.04  E-value: 1.81e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328  10 LFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVW-EEFGFTpsgNNLNKAVSELRKsfhALGDs 88
Cdd:COG0745  130 LLDLAAREVTRDGEP---VELTPKEFRLLELLMRNPGRVVSREQLLEEVWgYDYGDD---RTVDVHISRLRK---KLED- 199


                 ....*.
gi 495775328  89 hDYIVT 94
Cdd:COG0745  200 -DLIET 204
PRK11083 PRK11083
DNA-binding response regulator CreB; Provisional
 12-61 2.21e-03

DNA-binding response regulator CreB; Provisional


Pssm-ID: 236838 [Multi-domain]  Cd Length: 228  Bit Score: 38.40  E-value: 2.21e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 495775328  12 DPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEE 61
Cdd:PRK11083 140 DEPAARISYFGTP---LTLTRYEFLLLKTLLLSPGRVFSRQQLMDIVWED 186
PRK10161 PRK10161
phosphate response regulator transcription factor PhoB;
9-102 2.73e-03

phosphate response regulator transcription factor PhoB;


Pssm-ID: 182277 [Multi-domain]  Cd Length: 229  Bit Score: 38.16  E-value: 2.73e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 495775328   9 LLFDPEAHAISIVGQPesvLTLSAPAVRLLQEFIRHKGRDLSREELITRVWEEFGFTPSgNNLNKAVSELRKSFHALGds 88
Cdd:PRK10161 137 LSLDPTSHRVMAGEEP---LEMGPTEFKLLHFFMTHPERVYSREQLLNHVWGTNVYVED-RTVDVHIRRLRKALEPGG-- 210

                         90
                 ....*....|....*
gi 495775328  89 HDYIV-TVPRFGFRF 102
Cdd:PRK10161 211 HDRMVqTVRGTGYRF 225
DnrI COG3629
DNA-binding transcriptional regulator DnrI/AfsR/EmbR, SARP family, contains BTAD domain ...
 44-105 7.38e-03

DNA-binding transcriptional regulator DnrI/AfsR/EmbR, SARP family, contains BTAD domain [Transcription];


Pssm-ID: 442847 [Multi-domain]  Cd Length: 244  Bit Score: 36.74  E-value: 7.38e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 495775328  44 HKGRDLSREELITRVWEEfgfTPSG---NNLNKAVSELRKSFHALGDshDYIVTVPRfGFRFDAD 105
Cdd:COG3629   36 RAGRPVSRDRLADLLWGD---DPPAsarANLRTYVSRLRRALGPAGG--DLLVTRGG-GYRLDVD 94
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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