|
Name |
Accession |
Description |
Interval |
E-value |
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
6-648 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1334.39 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 6 LYPVRPEVAANTLTDEATYKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVkqtsFDDHHVDIKWFADGTLNVSYNCLDRH 85
Cdd:PRK00174 1 VFPPPAEFAANALIDMEQYKALYQESVEDPEGFWAEQAKRLDWFKPFDTV----LDWNAPFIKWFEDGELNVSYNCLDRH 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 86 LAERGDQAAIIWEGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGG 165
Cdd:PRK00174 77 LKTRGDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 166 FSPEALAGRIIDCKSKVVITADEGIRAGKKIPLKANVDDALTNPEtsSIQKVIVCKRTNGAIKWNQHRDIWYEDLMKVAG 245
Cdd:PRK00174 157 FSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALANCP--SVEKVIVVRRTGGDVDWVEGRDLWWHELVAGAS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 246 AVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGA 325
Cdd:PRK00174 235 DECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSYIVYGPLANGA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 326 TTLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQS 405
Cdd:PRK00174 315 TTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAWEWYYKVVGGE 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 406 RCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRFV 485
Cdd:PRK00174 395 RCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMRTIYGDHERFV 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 486 DTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTL 565
Cdd:PRK00174 475 KTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTL 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 566 NGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEYdGLGDISTLADPGVVAHLIETH 645
Cdd:PRK00174 555 KGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGEE-ILGDTSTLADPSVVEKLIEAR 633
|
...
gi 734849183 646 KTM 648
Cdd:PRK00174 634 QNR 636
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
18-644 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1165.86 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 18 LTDEATYKAMYQQSVVNPDGFWREQAKR-LDWIKPFTTVKQTSFDDhhvDIKWFADGTLNVSYNCLDRHLAERGDQAAII 96
Cdd:TIGR02188 1 IANLEQYKELYEESIEDPDKFWAKLARElLDWFKPFTKVLDWSFPP---FYKWFVGGELNVSYNCVDRHLEARPDKVAII 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 97 WEGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRII 176
Cdd:TIGR02188 78 WEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALADRIN 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 177 DCKSKVVITADEGIRAGKKIPLKANVDDALTNPETSsIQKVIVCKRTNG-AIKWNQHRDIWYEDLMKVAGAVCAPKEMGA 255
Cdd:TIGR02188 158 DAGAKLVITADEGLRGGKVIPLKAIVDEALEKCPVS-VEHVLVVRRTGNpVVPWVEGRDVWWHDLMAKASAYCEPEPMDS 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPN 335
Cdd:TIGR02188 237 EDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGATTVMFEGVPT 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 336 YPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRCPIVDTWWQ 415
Cdd:TIGR02188 317 YPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKERCPIVDTWWQ 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 416 TETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGN-LVILDSWPGQARTLYGDHDRFVDTYFKTFRG 494
Cdd:TIGR02188 397 TETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGPGEGGyLVIKQPWPGMLRTIYGDHERFVDTYFSPFPG 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 495 MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEA 574
Cdd:TIGR02188 477 YYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDE 556
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 575 LRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEYDGLGDISTLADPGVVAHLIET 644
Cdd:TIGR02188 557 LRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIEA 626
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
23-634 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1146.92 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 23 TYKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTSFDdhHVDIKWFADGTLNVSYNCLDRHLAERGDQAAIIWEGDDP 102
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFKPWDKVLDWSKG--PPFIKWFEGGKLNISYNCLDRHLKERGDKVAIIWEGDEP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05966 80 DQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRINDAQCKL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 VITADEGIRAGKKIPLKANVDDALTnpETSSIQKVIVCKRTNGAIKWNQHRDIWYEDLMKVAGAVCAPKEMGAEEALFIL 262
Cdd:cd05966 160 VITADGGYRGGKVIPLKEIVDEALE--KCPSVEKVLVVKRTGGEVPMTEGRDLWWHDLMAKQSPECEPEWMDSEDPLFIL 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDITRV 342
Cdd:cd05966 238 YTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFEGTPTYPDPGRY 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 343 AKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRCPIVDTWWQTETGGNM 422
Cdd:cd05966 318 WDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVDTWWQTETGGIM 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 423 MSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRFVDTYFKTFRGMYFTGDGA 502
Cdd:cd05966 398 ITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSKFPGYYFTGDGA 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 503 RRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNW 582
Cdd:cd05966 478 RRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDELRKELRKH 557
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 734849183 583 VRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEyDGLGDISTLAD 634
Cdd:cd05966 558 VRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
68-643 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 918.35 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 68 KWFADGTLNVSYNCLDRHLAERGDQAAIIWEGDDpAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVV 147
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGRGDKVALIWEGED-GEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 148 AMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIPLKANVDDALtnPETSSIQKVIVCKRTNGAI 227
Cdd:COG0365 80 AMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEAL--EELPSLEHVIVVGRTGADV 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 228 KWnqHRDIWYEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADV 307
Cdd:COG0365 158 PM--EGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADI 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 308 GWVTGHTYIVYGPLANGATTLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVG 387
Cdd:COG0365 236 GWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLRLLGSAG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 388 EPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGNMMSPLPGaHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIL 467
Cdd:COG0365 316 EPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 DSWPGQARTLYGDHDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV 547
Cdd:COG0365 392 GPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAV 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 548 VGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEydGLG 627
Cdd:COG0365 472 VGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEGR--PLG 549
|
570
....*....|....*.
gi 734849183 628 DISTLADPGVVAHLIE 643
Cdd:COG0365 550 DTSTLEDPEALDEIKE 565
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
24-611 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 853.41 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 24 YKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTSFDDHHVDIKWFADGTLNVSYNCLDRHLAERGDQAAIIWEGDDPA 103
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWITPYQKVKNTSFAPGAPSIKWFEDATLNLAANALDRHLRENGDRTAIIYEGDDTS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd17634 81 QSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITADEGIRAGKKIPLKANVDDALtNPETSSIQKVIVCKRTNGAIKWNQHRDIWYEDLMKVAGAVCAPKEMGAEEALFILY 263
Cdd:cd17634 161 ITADGGVRAGRSVPLKKNVDDAL-NPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQPEAMNAEDPLFILY 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDITRVA 343
Cdd:cd17634 240 TSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGVPNWPTPARMW 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 344 KIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRCPIVDTWWQTETGGNMM 423
Cdd:cd17634 320 QVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTWWQTETGGFMI 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 424 SPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRFVDTYFKTFRGMYFTGDGAR 503
Cdd:cd17634 400 TPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFKGMYFSGDGAR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWV 583
Cdd:cd17634 480 RDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPELYAELRNWV 559
|
570 580
....*....|....*....|....*...
gi 734849183 584 RKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:cd17634 560 RKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
6-643 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 813.75 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 6 LYPVRPEVAANTLTDEATYKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTS----FDDHHVDIKWFADGTLNVSYNC 81
Cdd:PLN02654 14 VFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWEGDEVCSenldVRKGPISIEWFKGGKTNICYNC 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHL-AERGDQAAIIWEGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHS 160
Cdd:PLN02654 94 LDRNVeAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHS 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 161 VVFGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIPLKANVDDALTNPETSSIQkVIVC---------KRTNgaIKWNQ 231
Cdd:PLN02654 174 VVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVS-VGICltyenqlamKRED--TKWQE 250
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 232 HRDIWYEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVT 311
Cdd:PLN02654 251 GRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWIT 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 312 GHTYIVYGPLANGATTLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPIN 391
Cdd:PLN02654 331 GHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLRVLGSVGEPIN 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 392 PEAWDWYYKNVGQSRCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWP 471
Cdd:PLN02654 411 PSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKSWP 490
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 472 GQARTLYGDHDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVP 551
Cdd:PLN02654 491 GAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIE 570
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 552 HDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEYDGLGDIST 631
Cdd:PLN02654 571 HEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGDTST 650
|
650
....*....|..
gi 734849183 632 LADPGVVAHLIE 643
Cdd:PLN02654 651 LADPGVVDQLIA 662
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
24-641 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 641.67 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 24 YKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQtsfDDHHVDIKWFADGTLNVSYNCLDRH-LAERGDQAAIIWEGDDP 102
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPPEKILD---NSNPPFTRWFVGGRLNTCYNALDRHvEAGRGDQIALIYDSPVT 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05967 78 GTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPKL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 VITADEGIRAGKKIPLKANVDDALTNPETSSIqKVIVCKRTNGAIKWNQH-RDIWYEDLMKVAGAV-CAPKEmgAEEALF 260
Cdd:cd05967 158 IVTASCGIEPGKVVPYKPLLDKALELSGHKPH-HVLVLNRPQVPADLTKPgRDLDWSELLAKAEPVdCVPVA--ATDPLY 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVP-NYPDI 339
Cdd:cd05967 235 ILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGKPvGTPDP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 340 TRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEG--ADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTE 417
Cdd:cd05967 315 GAFWRVIEKYQVNALFTAPTAIRAIRKEDPDGKYIkkYDLSSLRTLFLAGERLDPPTLEWAENTLGV---PVIDHWWQTE 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 418 TGGNMMSPLPG--AHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVI-LDSWPGQARTLYGDHDRFVDTYFKTFRG 494
Cdd:cd05967 392 TGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIkLPLPPGCLLTLWKNDERFKKLYLSKFPG 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 495 MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTL-NGGEEPTE 573
Cdd:cd05967 472 YYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLkEGVKITAE 551
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 574 ALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEydGLGDISTLADPGVVAHL 641
Cdd:cd05967 552 ELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIADGE--DYTIPSTIEDPSVLDEI 617
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
22-635 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 621.20 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 22 ATYKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTS---FddhhvdIKWFADGTLNVSYNCLDRHLAERGDQAAIIWE 98
Cdd:PRK10524 2 MSYSEFYQRSIDDPEAFWAEQARRIDWQTPFTQVLDYSnppF------ARWFVGGRTNLCHNAVDRHLAKRPEQLALIAV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 99 GDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDC 178
Cdd:PRK10524 76 STETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 179 KSKVVITADEGIRAGKKIPLKANVDDALTNPETSSiQKVIVCKRTNGAIKWNQHRDIWYEDL-MKVAGAVCAPKEMGAEE 257
Cdd:PRK10524 156 KPVLIVSADAGSRGGKVVPYKPLLDEAIALAQHKP-RHVLLVDRGLAPMARVAGRDVDYATLrAQHLGARVPVEWLESNE 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 258 ALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYP 337
Cdd:PRK10524 235 PSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMYEGLPTRP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 338 DITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTE 417
Cdd:PRK10524 315 DAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALGV---PVIDNYWQTE 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 418 TGGNMMSPLPGAHAL--KPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDS--WPGQARTLYGDHDRFVDTYFKTF- 492
Cdd:PRK10524 392 TGWPILAIARGVEDRptRLGSPGVPMYGYNVKLLNEVTGEPCGPNEKGVLVIEGplPPGCMQTVWGDDDRFVKTYWSLFg 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 493 RGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGG---E 569
Cdd:PRK10524 472 RQVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSdslA 551
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 570 EPTEALRLE--LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEydGLGDISTLADP 635
Cdd:PRK10524 552 DREARLALEkeIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIAEGR--DPGDLTTIEDP 617
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
22-635 |
0e+00 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 541.70 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 22 ATYKAMYQQSVVNPDGFWREQAKRLD--WIKPFTTVKQTSfdDHHVDIKWFADGTLNVSYNCLDRHLAERGDQAAIIWEG 99
Cdd:cd05968 7 PDLEAFLERSAEDNAWFWGEFVKDVGieWYEPPYQTLDLS--GGKPWAAWFVGGRMNIVEQLLDKWLADTRTRPALRWEG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDpAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:cd05968 85 ED-GTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 180 SKVVITADEGIRAGKKIPLKANVDDALTnpETSSIQKVIVCKRTNGAIKWNQHRDIWYEDLMKVAGAVCApkEMGAEEAL 259
Cdd:cd05968 164 AKALITADGFTRRGREVNLKEEADKACA--QCPTVEKVVVVRHLGNDFTPAKGRDLSYDEEKETAGDGAE--RTESEDPL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 260 FILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGE-IYWCTaDVGWVTGhTYIVYGPLANGATTLLFEGVPNYPD 338
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATMVLYDGAPDHPK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 339 ITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRCPIVDTWWQTET 418
Cdd:cd05968 318 ADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRNPIINYSGGTEI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 419 GGNMMSPLPgAHALKPGSAARPFFGVVPALVDNLGNLIEgPAEGNLVILDSWPGQARTLYGDHDRFVDTYFKTFRGMYFT 498
Cdd:cd05968 398 SGGILGNVL-IKPIKPSSFNGPVPGMKADVLDESGKPAR-PEVGELVLLAPWPGMTRGFWRDEDRYLETYWSRFDNVWVH 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLE 578
Cdd:cd05968 476 GDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTEALAEE 555
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 579 LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGEydGLGDISTLADP 635
Cdd:cd05968 556 LMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIRAAYLGK--ELGDLSSLENP 610
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
54-634 |
6.48e-169 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 494.41 E-value: 6.48e-169
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 54 TVKQTSFDDHHVDIKWFADGTLNVSYNCLDRHLA-ERGDQAAIIWEGDDPAEsrTITYRELHEEVCKFANALRGQDVHRG 132
Cdd:PRK04319 21 TYATFSWEEVEKEFSWLETGKVNIAYEAIDRHADgGRKDKVALRYLDASRKE--KYTYKELKELSNKFANVLKELGVEKG 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 133 DVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIRagkKIPLkanvddaltnPETS 212
Cdd:PRK04319 99 DRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLE---RKPA----------DDLP 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 213 SIQKVIVCKRTNGAIKwnqhRDIWYEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGgyllyAALTH--- 289
Cdd:PRK04319 166 SLKHVLLVGEDVEEGP----GTLDFNALMEQASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHN-----AMLQHyqt 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 290 -ERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGvpnYPDITRVAKIVDKHKVNILYTAPTAIRAMMASG 368
Cdd:PRK04319 237 gKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVIDGG---RFSPERWYRILEDYKVTVWYTAPTAIRMLMGAG 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 369 TAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTETGGNMMSPLPgAHALKPGSAARPFFGVVPAL 448
Cdd:PRK04319 314 DDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFGL---PIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAI 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 449 VDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRfvdtYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRM 527
Cdd:PRK04319 390 VDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEK----YESYFAGdWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERV 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 528 GTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRS 607
Cdd:PRK04319 466 GPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRS 545
|
570 580
....*....|....*....|....*....
gi 734849183 608 GKIMRRILRkiaTGEYdGL--GDISTLAD 634
Cdd:PRK04319 546 GKIMRRVLK---AWEL-GLpeGDLSTMED 570
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
109-619 |
6.62e-136 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 405.35 E-value: 6.62e-136
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 giragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiWYEdlmkvagavcapkEMGAEEALFILYTSGST 268
Cdd:cd05969 82 -----------------------------------------------LYE-------------RTDPEDPTLLHYTSGTT 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 269 GKPKGVQHTTGGYLLYAaLTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGvpnYPDITRVAKIVDK 348
Cdd:cd05969 102 GTPKGVLHVHDAMIFYY-FTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEG---RFDAESWYGIIER 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 349 HKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTETGGNMMSPLPG 428
Cdd:cd05969 178 VKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFGV---PIHDTWWQTETGSIMIANYPC 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 429 AHAlKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRFvDTYFKTfrGMYFTGDGARRDEDG 508
Cdd:cd05969 255 MPI-KPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERY-KNSFID--GWYLTGDLAYRDEDG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 509 YYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIG 588
Cdd:cd05969 331 YFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLG 410
|
490 500 510
....*....|....*....|....*....|.
gi 734849183 589 PIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd05969 411 AHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
109-617 |
1.33e-115 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 352.41 E-value: 1.33e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITade 188
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT--- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 giragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemGAEEALFILYTSGST 268
Cdd:cd05972 79 -----------------------------------------------------------------DAEDPALIYFTSGTT 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 269 GKPKGVQHTTGgYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDitRVAKIVDK 348
Cdd:cd05972 94 GLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDAE--RILELLER 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 349 HKVNILYTAPTAIRAMMASGTaacEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTETGgNMMSPLPG 428
Cdd:cd05972 171 YGVTSFCGPPTAYRMLIKQDL---SSYKFSHLRLVVSAGEPLNPEVIEWWRAATGL---PIRDGYGQTETG-LTVGNFPD 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 429 AhALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLYGDHDRfvdtYFKTFRG-MYFTGDGARRDED 507
Cdd:cd05972 244 M-PVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEK----TEASIRGdYYLTGDRAYRDED 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 508 GYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEI 587
Cdd:cd05972 319 GYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQGHVKKVL 398
|
490 500 510
....*....|....*....|....*....|
gi 734849183 588 GPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05972 399 APYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
82-523 |
4.32e-103 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 319.64 E-value: 4.32e-103
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIiwegdDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:pfam00501 1 LERQAARTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADEgiragkkiPLKANVDDALTNPETSsiqKVIVCKRTNGAIKWnqhrDIWYEDLM 241
Cdd:pfam00501 76 LNPRLPAEELAYILEDSGAKVLITDDA--------LKLEELLEALGKLEVV---KLVLVLDRDPVLKE----EPLPEEAK 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTgGYLLYAALTH----ERVFDYRPGEIYWCTADVGWVTGHTYIV 317
Cdd:pfam00501 141 PADVPPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGPLANGATTLLFEGVPNyPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADGSSLRLLGSVGEPINPEAWDW 397
Cdd:pfam00501 220 LGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAP--KRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKnvgQSRCPIVDTWWQTETGGNMMSPLPG-AHALKPGSAARPFFGVVPALVD-NLGNLIEGPAEGNLVIldSWPGQAR 475
Cdd:pfam00501 297 FRE---LFGGALVNGYGLTETTGVVTTPLPLdEDLRSLGSVGRPLPGTEVKIVDdETGEPVPPGEPGELCV--RGPGVMK 371
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 734849183 476 TLYGDHDRFVDTYFKTfrGMYFTGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:pfam00501 372 GYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
82-619 |
1.79e-96 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 303.66 E-value: 1.79e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:COG0318 5 LRRAAARHPDRPALVFGG------RRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITAdegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlm 241
Cdd:COG0318 79 LNPRLTAEELAYILEDSGARALVTA------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 kvagavcapkemgaeealFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPL 321
Cdd:COG0318 104 ------------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPL 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ANGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLGSVGEPINPEAWDWYYKN 401
Cdd:COG0318 165 LAGATLVLLPRF----DPERVLELIERERVTVLFGVPTMLARLLR--HPEFARYDLSSLRLVVSGGAPLPPELLERFEER 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 402 VGqsrCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDH 481
Cdd:COG0318 239 FG---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVV--RGPNVMKGYWNDP 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 DRFVdtyfKTFR-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIY 560
Cdd:COG0318 314 EATA----EAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVV 389
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 561 VYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:COG0318 390 AFVVLRPGAELDAE---ELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERY 445
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
13-643 |
1.17e-90 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 294.78 E-value: 1.17e-90
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 13 VAANTLTDEATYKAMYQQSVVNPDGFWREQAKRLDWI--KPFTTVKQtsfDDHHVDIKWFADGTLNVSYNCLdRHlaERG 90
Cdd:PRK03584 25 LAARRGLSFDDYAALWRWSVEDLEAFWQSVWDFFGVIgsTPYTVVLA---GRRMPGARWFPGARLNYAENLL-RH--RRD 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGDDpAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEA 170
Cdd:PRK03584 99 DRPAIIFRGED-GPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQG 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIDCKSKVVITADeGIR-AGKKIPLKANVD---DALTnpetsSIQKVIVCKRTNGAIKWNQHRDI--WYEDLMKVA 244
Cdd:PRK03584 178 VLDRFGQIEPKVLIAVD-GYRyGGKAFDRRAKVAelrAALP-----SLEHVVVVPYLGPAAAAAALPGAllWEDFLAPAE 251
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 245 GAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGE-IYWCTAdVGW------VTGhtyiv 317
Cdd:PRK03584 252 AAELEFEPVPFDHPLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPGDrFFWYTT-CGWmmwnwlVSG----- 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 ygpLANGATTLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDW 397
Cdd:PRK03584 326 ---LLVGATLVLYDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDW 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGQsrcpivDTWWQTETGG-NMMSPLPGAHALKP---GSAARPFFGVVPALVDNLGNLIEGpAEGNLVILDSWPGQ 473
Cdd:PRK03584 403 VYEHVKA------DVWLASISGGtDICSCFVGGNPLLPvyrGEIQCRGLGMAVEAWDEDGRPVVG-EVGELVCTKPFPSM 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 474 ARTLYGDHD--RFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVp 551
Cdd:PRK03584 476 PLGFWNDPDgsRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQ- 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 552 hDIKGQGIYV--YVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATG-EYDGLGD 628
Cdd:PRK03584 555 -EWPDGDVRMplFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVELPVKKLLHGrPVKKAVN 633
|
650
....*....|....*
gi 734849183 629 ISTLADPGVVAHLIE 643
Cdd:PRK03584 634 RDALANPEALDWFAD 648
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
257-611 |
1.13e-88 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 279.56 E-value: 1.13e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 257 EALFILYTSGSTGKPKGVQHTTGGYLLYAALThERVFDYRPGEIYWCTADVGWVtGHTYIVYGPLANGATTLLFEGvpny 336
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAAL-AASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 PDITRVAKIVDKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQT 416
Cdd:cd04433 75 FDPEAALELIEREKVTILLGVPTLLARLLK--APESAGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 417 ETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQartlyGDHDRFVDTYFKTFRGMY 496
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMK-----GYWNNPEATAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlr 576
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAE-- 302
|
330 340 350
....*....|....*....|....*....|....*
gi 734849183 577 lELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:cd04433 303 -ELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
22-622 |
4.02e-88 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 287.25 E-value: 4.02e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 22 ATYKAMYQQSVVNPDGFWREQAKRLD--WIKPFTTVKQTsfDDHHVDIKWFADGTLNVSYNCLdRHlAERGDQAAIIweG 99
Cdd:cd05943 17 ADYAALHRWSVDDPGAFWAAVWDFSGvrGSKPYDVVVVS--GRIMPGARWFPGARLNYAENLL-RH-ADADDPAAIY--A 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:cd05943 91 AEDGERTEVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQIE 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 180 SKVVITADEGIRAGKKIPLKANVddALTNPETSSIQKVIVCKRTNGAIKWN---QHRDIWYED-LMKVAGAVCAPKEMGA 255
Cdd:cd05943 171 PKVLFAVDAYTYNGKRHDVREKV--AELVKGLPSLLAVVVVPYTVAAGQPDlskIAKALTLEDfLATGAAGELEFEPLPF 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHtYIVYGpLANGATTLLFEGVPN 335
Cdd:cd05943 249 DHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWN-WLVSG-LAVGATIVLYDGSPF 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 336 YPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrcpivDTWWQ 415
Cdd:cd05943 327 YPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIKP------DVLLA 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 416 TETGG-NMMSPLPGAHALKP---GSAARPFFGVVPALVDNLGNLIEGPAeGNLVILDSWPGQARTLYGDHD--RFVDTYF 489
Cdd:cd05943 401 SISGGtDIISCFVGGNPLLPvyrGEIQCRGLGMAVEAFDEEGKPVWGEK-GELVCTKPFPSMPVGFWNDPDgsRYRAAYF 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 490 KTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGvpHDIKGQGIYV--YVTLNG 567
Cdd:cd05943 480 AKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVG--QEWKDGDERVilFVKLRE 557
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 568 GEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGE 622
Cdd:cd05943 558 GVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKIIAGR 612
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
108-617 |
5.81e-84 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 270.93 E-value: 5.81e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITad 187
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 egiragkkiplkaNVDdaltnpetssiqkvivckrtngaikwNQHRDiwyedlmkvagavcapkemgAEEALFILYTSGS 267
Cdd:cd05973 79 -------------DAA--------------------------NRHKL--------------------DSDPFVMMFTSGT 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 268 TGKPKGVQHTT------GGYLLYAalthervFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGvPNYPDITr 341
Cdd:cd05973 100 TGLPKGVPVPLralaafGAYLRDA-------VDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEG-GFSVEST- 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 342 vAKIVDKHKVNILYTAPTAIRAMMASGTAAcEGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGN 421
Cdd:cd05973 171 -WRVIERLGVTNLAGSPTAYRLLMAAGAEV-PARPKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQTELGMV 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 422 MMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIeGPAE-GNLVI-LDSWPGQARTLYGDHDRfvdtyfKTFRGMYF-T 498
Cdd:cd05973 246 LANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDEL-GPGEpGRLAIdIANSPLMWFRGYQLPDT------PAIDGGYYlT 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLE 578
Cdd:cd05973 319 GDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEGTPALADE 398
|
490 500 510
....*....|....*....|....*....|....*....
gi 734849183 579 LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05973 399 LQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
82-616 |
3.74e-76 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 252.29 E-value: 3.74e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAE-RGDQAAIIwegdDPAesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHS 160
Cdd:cd05959 9 VDLNLNEgRGDKTAFI----DDA--GSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 161 VVFGGFSPEALAGRIIDCKSKVVITADEgiragkkipLKANVDDALTNPETSsIQKVIVCkrtNGAIkwNQHRDIWYEDL 240
Cdd:cd05959 83 PVNTLLTPDDYAYYLEDSRARVVVVSGE---------LAPVLAAALTKSEHT-LVVLIVS---GGAG--PEAGALLLAEL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGyLLYAALTHER-VFDYRPGEIYWCTADVGWVTGHTYIVYG 319
Cdd:cd05959 148 VAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYARnVLGIREDDVCFSAAKLFFAYGLGNSLTF 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 320 PLANGATTLLFEGVPNyPDitRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEgaDGSSLRLLGSVGEPINPEAWDWYY 399
Cdd:cd05959 227 PLSVGATTVLMPERPT-PA--AVFKRIRRYRPTVFFGVPTLYAAMLAAPNLPSR--DLSSLRLCVSAGEALPAEVGERWK 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 400 KNVGqsrCPIVDTWWQTETGGNMMSPLPGAhaLKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQArTLYG 479
Cdd:cd05959 302 ARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYV--RGPSSA-TMYW 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 480 dHDRfvDTYFKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQG 558
Cdd:cd05959 374 -NNR--DKTRDTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTK 450
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 559 IYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05959 451 PKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
17-617 |
6.93e-72 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 244.65 E-value: 6.93e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 17 TLTDEATYKAMYQQSVVNPDGFWREQAKRL-DWIKPFTTVkqTSFDDHHVDikWFADGTLNVSYNCLDRHL--AERGDQA 93
Cdd:PTZ00237 3 KLSDPFDYENDSNYANSNPESFWDEVAKKYvHWDKMYDKV--YSGDEIYPD--WFKGGELNTCYNVLDIHVknPLKRDQD 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 94 AIIWEGddPAESRTI--TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEAL 171
Cdd:PTZ00237 79 ALIYEC--PYLKKTIklTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSL 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 172 AGRIIDCKSKVVITADEGIRAGKKIPLKANVDDALtnpETSSIQ--KVIVCKRtNGAIKWNQHRDI-----------WYE 238
Cdd:PTZ00237 157 IDRIETITPKLIITTNYGILNDEIITFTPNLKEAI---ELSTFKpsNVITLFR-NDITSESDLKKIetiptipntlsWYD 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAP----KEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHT 314
Cdd:PTZ00237 233 EIKKIKENNQSPfyeyVPVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVSFHG 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 315 YIvYGPLANGATTLLFEG--VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMAS---GTAACEGADGSSLRLLGSVGEP 389
Cdd:PTZ00237 313 FL-YGSLSLGNTFVMFEGgiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIKTdpeATIIRSKYDLSNLKEIWCGGEV 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 390 INPEAWDwYYKNVGQSRCPIVdtWWQTETGgnMMSPLPGAHALKPGSA-ARPFFGVVPALVDNLGNLIEGPAEGNLVI-L 467
Cdd:PTZ00237 392 IEESIPE-YIENKLKIKSSRG--YGQTEIG--ITYLYCYGHINIPYNAtGVPSIFIKPSILSEDGKELNVNEIGEVAFkL 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 DSWPGQARTLYGDHDRFvDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV 547
Cdd:PTZ00237 467 PMPPSFATTFYKNDEKF-KQLFSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCS 545
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 548 VGVPH-DIKGQGIYVYVTLNGGEEPT---EALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PTZ00237 546 IGIYDpDCYNVPIGLLVLKQDQSNQSidlNKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
106-616 |
9.48e-72 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 238.52 E-value: 9.48e-72
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05919 9 RSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVVT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEgiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagAVCapkemgaeealFILYTS 265
Cdd:cd05919 89 SAD---------------------------------------------------------DIA-----------YLLYSS 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADV--GWVTGHTyiVYGPLANGATTLLFegvPNYPDITRVA 343
Cdd:cd05919 101 GTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLN---PGWPTAERVL 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 344 KIVDKHKVNILYTAPTAIRAMMASGtaACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGNMM 423
Cdd:cd05919 176 ATLARFRPTVLYGVPTFYANLLDSC--AGSPDALRSLRLCVSAGEALPRGLGERWMEHFG---GPILDGIGATEVGHIFL 250
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 424 SPLPGAhaLKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYgdhdRFVDTYFKTFR-GMYFTGDGA 502
Cdd:cd05919 251 SNRPGA--WRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLV--RGPSAAVGYW----NNPEKSRATFNgGWYRTGDKF 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 503 RRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNW 582
Cdd:cd05919 323 CRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDIHRH 402
|
490 500 510
....*....|....*....|....*....|....
gi 734849183 583 VRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05919 403 LLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
60-616 |
4.03e-70 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 237.01 E-value: 4.03e-70
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 60 FDDHHVDIKWFADGTLNVSYNCLDRHLAERGDQAAIIWeGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYM 139
Cdd:cd05970 1 YEDFHNNFSINVPENFNFAYDVVDAMAKEYPDKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 140 PMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIragkkipLKANVDDALtnPETSSIQKVIV 219
Cdd:cd05970 80 KRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAEDN-------IPEEIEKAA--PECPSKPKLVW 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 220 CKrtngaikwNQHRDIW---YEDLMKVAGAV---CAPKEMGAEEALFILYTSGSTGKPKGVQHTTG---GYLLYAALTHe 290
Cdd:cd05970 151 VG--------DPVPEGWidfRKLIKNASPDFerpTANSYPCGEDILLVYFSSGTTGMPKMVEHDFTyplGHIVTAKYWQ- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 291 rvfDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDitRVAKIVDKHKVNILYTAPTAIRAMMasgTA 370
Cdd:cd05970 222 ---NVREGGLHLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDPK--ALLEKLSKYGVTTFCAPPTIYRFLI---RE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 371 ACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSrcpIVDTWWQTETGGNMMSpLPGAHAlKPGSAARPFFGVVPALVD 450
Cdd:cd05970 294 DLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIK---LMEGFGQTETTLTIAT-FPWMEP-KPGSMGKPAPGYEIDLID 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 451 NLGNLIEGPAEGNLVI--LDSWP-GQARTLYGDHDRFVDTYFKtfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRM 527
Cdd:cd05970 369 REGRSCEAGEEGEIVIrtSKGKPvGLFGGYYKDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRI 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 528 GTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRS 607
Cdd:cd05970 446 GPFEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTIS 525
|
....*....
gi 734849183 608 GKIMRRILR 616
Cdd:cd05970 526 GKIRRVEIR 534
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
109-616 |
1.03e-67 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 228.09 E-value: 1.03e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAde 188
Cdd:cd05971 8 TFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVTD-- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 giragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemGAEEALFILYTSGST 268
Cdd:cd05971 86 -----------------------------------------------------------------GSDDPALIIYTSGTT 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 269 GKPKGVQHTTG---GYLLYAALTHErvFDYRPGEIYWCTADVGWVtghtyivyGPLANGATTLLFEGVPNYP------DI 339
Cdd:cd05971 101 GPPKGALHAHRvllGHLPGVQFPFN--LFPRDGDLYWTPADWAWI--------GGLLDVLLPSLYFGVPVLAhrmtkfDP 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 340 TRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGadGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETG 419
Cdd:cd05971 171 KAALDLMSRYGVTTAFLPPTALKMMRQQGEQLKHA--QVKLRAIATGGESLGEELLGWAREQFG---VEVNEFYGQTECN 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 420 ---GNMMSPLPgahaLKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVI--------LDSWPGQARTlygdHDRFVDTY 488
Cdd:cd05971 246 lviGNCSALFP----IKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVelpdpvafLGYWNNPSAT----EKKMAGDW 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 489 FKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGG 568
Cdd:cd05971 318 LLT-------GDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPG 390
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 734849183 569 EEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05971 391 ETPSDALAREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
88-617 |
7.61e-66 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 225.42 E-value: 7.61e-66
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 88 ERGDQAAIIWEGDDPAESRTiTYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGF 166
Cdd:cd05928 23 KRPPNPALWWVNGKGDEVKW-SFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQL 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 167 SPEALAGRIIDCKSKVVITADEgiragkkipLKANVDD-ALTNPETSSiqKVIVCkrtngaikwNQHRDIW--YEDLMKV 243
Cdd:cd05928 102 TAKDILYRLQASKAKCIVTSDE---------LAPEVDSvASECPSLKT--KLLVS---------EKSRDGWlnFKELLNE 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 244 AGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLAN 323
Cdd:cd05928 162 ASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQ 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 324 GATTLLFEgVPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGadgSSLRLLGSVGEPINPEAWDWYYKNVG 403
Cdd:cd05928 242 GACVFVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQDLSSYKF---PSLQHCVTGGEPLNPEVLEKWKAQTG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 404 QSrcpIVDTWWQTETGgnMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSwPGQARTLYGDHDR 483
Cdd:cd05928 317 LD---IYEGYGQTETG--LICANFKGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGIRVK-PIRPFGLFSGYVD 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 484 FVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVY 562
Cdd:cd05928 391 NPEKTAATIRGdFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAF 470
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 563 VTLNG---GEEPtEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05928 471 VVLAPqflSHDP-EQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
22-617 |
3.45e-64 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 225.34 E-value: 3.45e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 22 ATYKAMYQQSVVNPDGFWREQAKRLDWI---KPFTTVKQTSFDDHhvDIKWFADGTLNVSYNCLDRHLAERGDQAAIIW- 97
Cdd:PLN03052 120 SSFSEFQRFSVENPEVYWSIVLDELSLVfsvPPRCILDTSDESNP--GGQWLPGAVLNVAECCLTPKPSKTDDSIAIIWr 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 98 -EGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRII 176
Cdd:PLN03052 198 dEGSDDLPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLK 277
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 177 DCKSKVVITADEGIRAGKKIPLKANVDDAlTNPetssiqKVIVCKRTNGAIKWN-QHRDIWYEDLMKVAGAVCAPKE--- 252
Cdd:PLN03052 278 ISKAKAIFTQDVIVRGGKSIPLYSRVVEA-KAP------KAIVLPADGKSVRVKlREGDMSWDDFLARANGLRRPDEyka 350
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 253 --MGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALT--HervFDYRPGEIY-WCTaDVGWVTGHtYIVYGPLANGATT 327
Cdd:PLN03052 351 veQPVEAFTNILFSSGTTGEPKAIPWTQLTPLRAAADAwaH---LDIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATL 425
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 328 LLFEGVPNYPDItrvAKIVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINPEAWDW-----YYKnv 402
Cdd:PLN03052 426 ALYNGSPLGRGF---AKFVQDAKVTMLGTVPSIVKTWKNTNCMA--GLDWSSIRCFGSTGEASSVDDYLWlmsraGYK-- 498
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 gqsrcPIVDTWWQTETG-----GNMMSPlpgaHALKPGSAARPFFGVVpaLVDNLGNLI--EGPAEGNLVILDSWPGQAR 475
Cdd:PLN03052 499 -----PIIEYCGGTELGggfvtGSLLQP----QAFAAFSTPAMGCKLF--ILDDSGNPYpdDAPCTGELALFPLMFGASS 567
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 476 TLY-GDHDrfvDTYFK---TFRGMYFT--GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAM-VAHPKVAEAAVV 548
Cdd:PLN03052 568 TLLnADHY---KVYFKgmpVFNGKILRrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAI 644
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 549 GVPHdiKGQG------IYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PLN03052 645 GVPP--PGGGpeqlviAAVLKDPPGSNPDLNELKKIFNSAIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ 717
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
79-616 |
1.03e-63 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 219.29 E-value: 1.03e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 79 YNCLDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAI 158
Cdd:PRK06187 9 GRILRHGARKHPDKEAVYFDG------RRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFAVPKIGAV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 159 -HSV-VFggFSPEALAGRIIDCKSKVVITADEGiragkkIPLKANVDDALTnpetsSIQKVIVckRTNGAIKWNQHRDIW 236
Cdd:PRK06187 83 lHPInIR--LKPEEIAYILNDAEDRVVLVDSEF------VPLLAAILPQLP-----TVRTVIV--EGDGPAAPLAPEVGE 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 237 YEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHttggyllyaalTHERVFDYRPGEIYWctadVGWVTGHTYI 316
Cdd:PRK06187 148 YEELLAAASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVL-----------SHRNLFLHSLAVCAW----LKLSRDDVYL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 317 VYGPLAN-GATTL----LFEGVPN-YP---DITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGSVG 387
Cdd:PRK06187 213 VIVPMFHvHAWGLpylaLMAGAKQvIPrrfDPENLLDLIETERVTFFFAVPTIWQMLLKA--PRAYFVDFSSLRLVIYGG 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 388 EPINP---EAWDWYYKnvgqsrCPIVDTWWQTETGGNM-MSPLP---GAHALKPGSAARPFFGVVPALVDNLGNLIE--G 458
Cdd:PRK06187 291 AALPPallREFKEKFG------IDLVQGYGMTETSPVVsVLPPEdqlPGQWTKRRSAGRPLPGVEARIVDDDGDELPpdG 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 459 PAEGNLVILDSWPGQArtLYGDHDrfvDTYfKTFR-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMV 537
Cdd:PRK06187 365 GEVGEIIVRGPWLMQG--YWNRPE---ATA-ETIDgGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELEDALY 438
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 538 AHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK06187 439 GHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAK---ELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKRVLR 514
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
82-616 |
2.88e-61 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 211.27 E-value: 2.88e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAI--- 158
Cdd:cd05936 5 LEEAARRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVvvp 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 159 HSVVFGgfsPEALAGRIIDCKSKVVITadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrDIWYE 238
Cdd:cd05936 79 LNPLYT---PRELEHILNDSGAKALIV------------------------------------------------AVSFT 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFD-YRPGEIYWCTADVGWVTGHTYIV 317
Cdd:cd05936 108 DLLAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDlLEGDDVVLAALPLFHVFGLTVAL 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGPLANGATTLLfegVPNYPDItRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGSVGEPINPEAWDW 397
Cdd:cd05936 188 LLPLALGATIVL---IPRFRPI-GVLKEIRKHRVTIFPGVPTMYIALLNA--PEFKKRDFSSLRLCISGGAPLPVEVAER 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGqsrCPIVDTWWQTETggnmmSPLPGAHAL----KPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVI------L 467
Cdd:cd05936 262 FEELTG---VPIVEGYGLTET-----SPVVAVNPLdgprKPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVrgpqvmK 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 DSWPGQARTlygdHDRFVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV 547
Cdd:cd05936 334 GYWNRPEET----AEAFVDGWLRT-------GDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAV 402
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 548 VGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05936 403 VGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
82-612 |
6.65e-61 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 209.39 E-value: 6.65e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:cd17631 1 LRRRARRHPDRTALVFGG------RSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVP 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVItadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyEDLM 241
Cdd:cd17631 75 LNFRLTPPEVAYILADSGAKVLF-----------------------------------------------------DDLA 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 kvagavcapkemgaeealFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPL 321
Cdd:cd17631 102 ------------------LLMYTSGTTGRPKGAMLTHRN-LLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTL 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ANGATTLLFEGvpnyPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINP---EAWDWY 398
Cdd:cd17631 163 LRGGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFA--TTDLSSLRAVIYGGAPMPErllRALQAR 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YknvgqsrCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARtly 478
Cdd:cd17631 237 G-------VKFVQGYGMTETSPGVTFLSPEDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVV--RGPHVMA--- 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 GDHDRFVDTYfKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ 557
Cdd:cd17631 305 GYWNRPEATA-AAFRDGWFhTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGE 383
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 558 GIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17631 384 AVVAVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
106-616 |
1.33e-56 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 198.09 E-value: 1.33e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHR-GDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVvi 184
Cdd:cd05958 9 REWTYRDLLALANRIANVLVGELGIVpGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARITV-- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 185 tadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagAVCAPKEMGAEEALFILYT 264
Cdd:cd05958 87 -------------------------------------------------------------ALCAHALTASDDICILAFT 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 265 SGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGvpNYPDitRVAK 344
Cdd:cd05958 106 SGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEE--ATPD--LLLS 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 345 IVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGNMMS 424
Cdd:cd05958 182 AIARYKPTVLFTAPTAYRAMLAHPDAA--GPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTEMFHIFIS 256
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 425 PLPGAhaLKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldswpgQARTLY-GDHDRFVDTYFKTfrGMYFTGDGAR 503
Cdd:cd05958 257 ARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAV------RGPTGCrYLADKRQRTYVQG--GWNITGDTYS 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWV 583
Cdd:cd05958 327 RDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHA 406
|
490 500 510
....*....|....*....|....*....|...
gi 734849183 584 RKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05958 407 KAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
91-613 |
5.93e-56 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 196.59 E-value: 5.93e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVfggfSPEA 170
Cdd:cd05930 2 DAVAVVDGD------QSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPL----DPSY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRII----DCKSKVVITadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnQHRDIWYedlmkvaga 246
Cdd:cd05930 72 PAERLAyileDSGAKLVLT---------------------------------------------DPDDLAY--------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 247 vcapkemgaeealfILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTA----DVGWVTghtyiVYGPLA 322
Cdd:cd05930 98 --------------VIYTSGSTGKPKGVMVEHRG-LVNLLLWMQEAYPLTPGDRVLQFTsfsfDVSVWE-----IFGALL 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 323 NGATTLLfegVPN--YPDITRVAKIVDKHKVNILYTAPTAIRAMMAsgtaACEGADGSSLRLLGSVGEPINPEAWDWYYK 400
Cdd:cd05930 158 AGATLVV---LPEevRKDPEALADLLAEEGITVLHLTPSLLRLLLQ----ELELAALPSLRLVLVGGEALPPDLVRRWRE 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 401 NVGQSRcpIVDTWWQTETGGNMmsplpGAHALKPGSAA-------RPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQ 473
Cdd:cd05930 231 LLPGAR--LVNLYGPTEATVDA-----TYYRVPPDDEEdgrvpigRPIPNTRVYVLDENLRPVPPGVPGELYI--GGAGL 301
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 474 ARTLYGDHD----RFVDTYFKTFRGMYFTGDGARRDEDG--YYwiTGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV 547
Cdd:cd05930 302 ARGYLNRPEltaeRFVPNPFGPGERMYRTGDLVRWLPDGnlEF--LGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAV 379
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 548 VGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRR 613
Cdd:cd05930 380 VAREDGDGEKRLVAYVVPDEGGELDEE---ELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
101-611 |
3.76e-55 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 195.51 E-value: 3.76e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKS 180
Cdd:cd05911 4 DADTGKELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKP 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 181 KVVITADEGIragkkiplkANVDDALTNPetSSIQKVIVckrTNGAIKWNQHRDIWYEDLMKVAGA-VCAPKEMGAEEAL 259
Cdd:cd05911 84 KVIFTDPDGL---------EKVKEAAKEL--GPKDKIIV---LDDKPDGVLSIEDLLSPTLGEEDEdLPPPLKDGKDDTA 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 260 FILYTSGSTGKPKGVQhttggyllyaaLTHER-------VFDYRPGEIYWCTADVG-----WVTGHTYIVYGPLaNGATT 327
Cdd:cd05911 150 AILYSSGTTGLPKGVC-----------LSHRNlianlsqVQTFLYGNDGSNDVILGflplyHIYGLFTTLASLL-NGATV 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 328 LLFegvpNYPDITRVAKIVDKHKVNILYTAPtAIRAMMASgTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVgqSRC 407
Cdd:cd05911 218 IIM----PKFDSELFLDLIEKYKITFLYLVP-PIAAALAK-SPLLDKYDLSSLRVILSGGAPLSKELQELLAKRF--PNA 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 408 PIVDTWWQTETGGnMMSPLPGaHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDS---WPG-------QARTL 477
Cdd:cd05911 290 TIKQGYGMTETGG-ILTVNPD-GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEPGEICVRGpqvMKGyynnpeaTKETF 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 478 ygDHDRFvdtyfktFRgmyfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ 557
Cdd:cd05911 368 --DEDGW-------LH----TGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGE 434
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 558 GIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDV-IQWAPGLPKTRSGKIM 611
Cdd:cd05911 435 LPRAYVVRKPGEKLTEK---EVKDYVAKKVASYKQLRGgVVFVDEIPKSASGKIL 486
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
73-617 |
7.78e-52 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 186.58 E-value: 7.78e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 73 GTLNVSYNCLDRHLAE-RGDQAAIIwegdDPAESrtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLA 151
Cdd:TIGR02262 1 EKYNAAEDLLDRNVVEgRGGKTAFI----DDISS--LSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 152 CARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEgiragkkipLKANVDDALTNPETSsIQKVIVCKRTNGAIKwnq 231
Cdd:TIGR02262 75 AIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGA---------LLPVIKAALGKSPHL-EHRVVVGRPEAGEVQ--- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 232 hrdiwYEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVT 311
Cdd:TIGR02262 142 -----LAELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAY 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 312 GHTYIVYGPLANGATTLLFegvPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTaaCEGADGSSLRLLGSVGEPIN 391
Cdd:TIGR02262 217 GLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPN--LPSEDQVRLRLCTSAGEALP 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 392 PEAWDWYYKNVGQSrcpIVDTWWQTETGGNMMSPLPgaHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWP 471
Cdd:TIGR02262 292 AEVGQRWQARFGVD---IVDGIGSTEMLHIFLSNLP--GDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLI--SGP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 472 GQARTLYGDHDRFVDTyfktFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:TIGR02262 365 SSATMYWNNRAKSRDT----FQGEWTrSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGV 440
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 551 P---HDIKGQGiyvYVTLNGGEeptEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:TIGR02262 441 AdedGLIKPKA---FVVLRPGQ---TALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
102-617 |
1.65e-49 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 180.20 E-value: 1.65e-49
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 102 PAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSK 181
Cdd:cd05926 9 PGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSK 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 182 VVITADEG----IRAGKKiplkanvddaltnpETSSIQKVIVCKRTNgaIKWNQHRDIWYEDlmKVAGAVCAPKEMGAEE 257
Cdd:cd05926 89 LVLTPKGElgpaSRAASK--------------LGLAILELALDVGVL--IRAPSAESLSNLL--ADKKNAKSEGVPLPDD 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 258 ALFILYTSGSTGKPKGVQhttggyllyaaLTHERVfdyrpgeiywCTADVGWVTGH-------TYIVYgPL--ANG--AT 326
Cdd:cd05926 151 LALILHTSGTTGRPKGVP-----------LTHRNL----------AASATNITNTYkltpddrTLVVM-PLfhVHGlvAS 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 TL--LFEG----VPNYPDITRVAKIVDKHKVNiLYTA-PTAIRAMMASGTAACEGADgSSLRLLGSVGEPINPEAWDWYY 399
Cdd:cd05926 209 LLstLAAGgsvvLPPRFSASTFWPDVRDYNAT-WYTAvPTIHQILLNRPEPNPESPP-PKLRFIRSCSASLPPAVLEALE 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 400 KNVGqsrCPIVDTWWQTETGGNMMS-PLPGAHAlKPGSAARPFfGVVPALVDNLGNLIEGPAEGNLVILDswPGQARTLY 478
Cdd:cd05926 287 ATFG---APVLEAYGMTEAAHQMTSnPLPPGPR-KPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRG--PNVTRGYL 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 GDHD-----RFVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHD 553
Cdd:cd05926 360 NNPEanaeaAFKDGWFRT-------GDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDE 432
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 554 IKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05926 433 KYGEEVAAAVVLREGASVTEE---ELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
108-619 |
3.58e-48 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 175.06 E-value: 3.58e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIiDCKSKVVITAD 187
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRV-DRGGAVYAAVD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EGIRAgkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgaEEALFILYTSGS 267
Cdd:cd05974 80 ENTHA---------------------------------------------------------------DDPMLLYFTSGT 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 268 TGKPKGVQHTTGGYLLyAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFegvpNYP--DITRVAKI 345
Cdd:cd05974 97 TSKPKLVEHTHRSYPV-GHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLF----NYArfDAKRVLAA 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 346 VDKHKVNILYTAPTAIRAMMASGTAACEGAdgssLRLLGSVGEPINPEAWDWYYKNVGQSrcpIVDTWWQTETGGnMMSP 425
Cdd:cd05974 172 LVRYGVTTLCAPPTVWRMLIQQDLASFDVK----LREVVGAGEPLNPEVIEQVRRAWGLT---IRDGYGQTETTA-LVGN 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPGaHALKPGSAARPFFGVVPALVDNLGNLI-EGpaEGNLVILDSWP-GQARTLYGDHDRfvdTYFKTFRGMYFTGDGAR 503
Cdd:cd05974 244 SPG-QPVKAGSMGRPLPGYRVALLDPDGAPAtEG--EVALDLGDTRPvGLMKGYAGDPDK---TAHAMRGGYYRTGDIAM 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWV 583
Cdd:cd05974 318 RDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFRFS 397
|
490 500 510
....*....|....*....|....*....|....*.
gi 734849183 584 RKEIGPIASPDVIQWAPgLPKTRSGKIMRRILRKIA 619
Cdd:cd05974 398 RERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
139-616 |
4.75e-48 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 176.16 E-value: 4.75e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 139 MPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIPLKANVDDAltnpeTSSIQKVI 218
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKVVEA-----APAKAIVL 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 219 VCKRTNGAIKWnQHRDIWYEDLMKVA-------GAVCAPKEMGAEEALFILYTSGSTGKPKGV--QHTTGgylLYAALTH 289
Cdd:PLN03051 76 PAAGEPVAVPL-REQDLSWCDFLGVAaaqgsvgGNEYSPVYAPVESVTNILFSSGTTGEPKAIpwTHLSP---LRCASDG 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 290 ERVFDYRPGEIYWCTADVGWVTGhTYIVYGPLANGATTLLFEGVPNYPDItrvAKIVDKHKVNILYTAPTAIRAMMASGT 369
Cdd:PLN03051 152 WAHMDIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGF---GKFVQDAGVTVLGLVPSIVKAWRHTGA 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 370 AACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRcPIVDTWWQTETGGNMMSPLPgAHALKPGSAARPFFGVVPALV 449
Cdd:PLN03051 228 FAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYISSTL-LQPQAPGAFSTASLGTRFVLL 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 450 DNLGNLI--EGPAEGNLVILDSWPGQA-RTLYGDHDRfvdTYFKTFRGMYFTGDGARRDED-------GYYWITGRVDDV 519
Cdd:PLN03051 306 NDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHDK---VYYKGMPMYGSKGMPLRRHGDimkrtpgGYFCVQGRADDT 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 520 LNVSGHRMGTAEIESAMV-AHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPT------EALRLELKNWVRKEIGPIAS 592
Cdd:PLN03051 383 MNLGGIKTSSVEIERACDrAVAGIAETAAVGVAPPDGGPELLVIFLVLGEEKKGfdqarpEALQKKFQEAIQTNLNPLFK 462
|
490 500
....*....|....*....|....
gi 734849183 593 PDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PLN03051 463 VSRVKIVPELPRNASNKLLRRVLR 486
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
79-641 |
5.58e-48 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 178.61 E-value: 5.58e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 79 YNCLDRHLAERGDQAAIIW--EGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIG 156
Cdd:PRK07529 28 YELLSRAAARHPDAPALSFllDADPLDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWGGEAAG 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 157 aIHSVVFGGFSPEALAGRIIDCKSKVVITA--DEGIRAGKKI--------PLKANVDDALTN---PETSSIQKVIVCKRT 223
Cdd:PRK07529 108 -IANPINPLLEPEQIAELLRAAGAKVLVTLgpFPGTDIWQKVaevlaalpELRTVVEVDLARylpGPKRLAVPLIRRKAH 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 224 NGAIKWNQHRDIWYEDLMkVAGAVCAPKEMGAeeaLFilYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWC 303
Cdd:PRK07529 187 ARILDFDAELARQPGDRL-FSGRPIGPDDVAA---YF--HTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFC 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 304 TADVGWVTGHTYIVYGPLANGATTLL-----FEGvPNYpdITRVAKIVDKHKVNILYTAPTAIRAMMAsgtAACEGADGS 378
Cdd:PRK07529 260 GLPLFHVNALLVTGLAPLARGAHVVLatpqgYRG-PGV--IANFWKIVERYRINFLSGVPTVYAALLQ---VPVDGHDIS 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 379 SLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTE-TGGNMMSPLPGAhaLKPGSAARPFFG-------------- 443
Cdd:PRK07529 334 SLRYALCGAAPLPVEVFRRFEAATGV---RIVEGYGLTEaTCVSSVNPPDGE--RRIGSVGLRLPYqrvrvvilddagry 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 444 VVPALVDNLGNL-IEGP--AEGNLVildswPGQARTLYGDhDRFVDTyfktfrgmyftGDGARRDEDGYYWITGRVDDVL 520
Cdd:PRK07529 409 LRDCAVDEVGVLcIAGPnvFSGYLE-----AAHNKGLWLE-DGWLNT-----------GDLGRIDADGYFWLTGRAKDLI 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 521 NVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIG-PIASPDVIQWA 599
Cdd:PRK07529 472 IRGGHNIDPAAIEEALLRHPAVALAAAVGRPDAHAGELPVAYVQLKPGASATEA---ELLAFARDHIAeRAAVPKHVRIL 548
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|...
gi 734849183 600 PGLPKTRSGKI---------MRRILRKIATGEYDGLGDISTLAD--PGVVAHL 641
Cdd:PRK07529 549 DALPKTAVGKIfkpalrrdaIRRVLRAALRDAGVEAEVVDVVEDgrRGLVAQV 601
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
91-617 |
2.78e-47 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 174.35 E-value: 2.78e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEA 170
Cdd:PRK08316 26 DKTALVFGD------RSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIDCKSKVVITADEgiragkkipLKANVDDALTNPETSSIQKVIVCKRTNGAIKWNQHRDiWYEdlmkvAGAVCAP 250
Cdd:PRK08316 100 LAYILDHSGARAFLVDPA---------LAPTAEAALALLPVDTLILSLVLGGREAPGGWLDFAD-WAE-----AGSVAEP 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 251 KEMGAEEALF-ILYTSGSTGKPKGVQHTTGgyllyaALTHERV-----FDYRPGEI-------YWCTA-DVgwvtghtyi 316
Cdd:PRK08316 165 DVELADDDLAqILYTSGTESLPKGAMLTHR------ALIAEYVscivaGDMSADDIplhalplYHCAQlDV--------- 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 317 VYGP-LANGATTLLFEGvpnyPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLlGSVGEPINPEAw 395
Cdd:PRK08316 230 FLGPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRH--PDFDTRDLSSLRK-GYYGASIMPVE- 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 396 dwyYKNVGQSRCPIVDTW---WQTEtggnmMSPL-----PGAHALKPGSAARPFFGVVPALVDNLGNLIegpAEGNL-VI 466
Cdd:PRK08316 302 ---VLKELRERLPGLRFYncyGQTE-----IAPLatvlgPEEHLRRPGSAGRPVLNVETRVVDDDGNDV---APGEVgEI 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 467 LDSWPgQARTLYGDHDRfvdtyfKT---FRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKV 542
Cdd:PRK08316 371 VHRSP-QLMLGYWDDPE------KTaeaFRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALYTHPAV 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 543 AEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK08316 444 AEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
106-616 |
5.40e-46 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 168.62 E-value: 5.40e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVit 185
Cdd:cd05934 2 RRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLV-- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 adegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagaVCAPKEmgaeealfILYTS 265
Cdd:cd05934 80 -------------------------------------------------------------VVDPAS--------ILYTS 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQhTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLfegVPNYpDITRVAKI 345
Cdd:cd05934 91 GTTGPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRF-SASRFWSD 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 346 VDKHKVNILYTAPTAIRAMMAsgtaACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGNMMSP 425
Cdd:cd05934 166 VRRYGATVTNYLGAMLSYLLA----QPPSPDDRAHRLRAAYGAPNPPELHEEFEERFG---VRLLEGYGMTETIVGVIGP 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPgaHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIlDSWPGQARTLyGDHDRFVDTYfKTFRGMYF-TGDGARR 504
Cdd:cd05934 239 RD--EPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVI-RGLRGWGFFK-GYYNMPEATA-EAMRNGWFhTGDLGYR 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 505 DEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVR 584
Cdd:cd05934 314 DADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE---ELFAFCE 390
|
490 500 510
....*....|....*....|....*....|..
gi 734849183 585 KEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05934 391 GQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
107-617 |
1.21e-45 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 167.94 E-value: 1.21e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 107 TITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITA 186
Cdd:cd05903 1 RLTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVP 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 187 DEgiragkkiplkanvddaltnpetssiqkvivckrtngaikWNQHRdiwyedlmkvagavcaPKEMGAEEALfILYTSG 266
Cdd:cd05903 81 ER----------------------------------------FRQFD----------------PAAMPDAVAL-LLFTSG 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 267 STGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVpnypDITRVAKIV 346
Cdd:cd05903 104 TTGEPKGVMHSHNT-LSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIW----DPDKALALM 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 347 DKHKVNILYTAPT----AIRAMMASGTAACegadgsSLRLLGSVGEPINP----EAWDWYYKNVgqsrcpiVDTWWQTET 418
Cdd:cd05903 179 REHGVTFMMGATPfltdLLNAVEEAGEPLS------RLRTFVCGGATVPRslarRAAELLGAKV-------CSAYGSTEC 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 419 GGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDswpgqARTLYGDHDRFVDTYFKTFRGMYFT 498
Cdd:cd05903 246 PGAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG-----PSVFLGYLDRPDLTADAAPEGWFRT 320
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPT-EALRL 577
Cdd:cd05903 321 GDLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTfDELVA 400
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 734849183 578 ELKnwvRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05903 401 YLD---RQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
82-617 |
2.21e-45 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 168.93 E-value: 2.21e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK07656 11 LARAARRFGDKEAYVFGD------QRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIPlkanvddaltnPETSSIQKVIVCKRTNGAIKWNQHRDiwYEDLM 241
Cdd:PRK07656 85 LNTRYTADEAAYILARGDAKALFVLGLFLGVDYSAT-----------TRLPALEHVVICETEEDDPHTEKMKT--FTDFL 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQhTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPL 321
Cdd:PRK07656 152 AAGDPAERAPEVDPDDVADILFTSGTTGRPKGAM-LTHRQLLSNAADWAEYLGLTEGDRYLAANPFFHVFGYKAGVNAPL 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ANGATTLLfegVPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEgaDGSSLRLLGSVGEPINPEAWDWYYKN 401
Cdd:PRK07656 231 MRGATILP---LPVF-DPDEVFRLIETERITVLPGPPTMYNSLLQHPDRSAE--DLSSLRLAVTGAASMPVALLERFESE 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 402 VGqsrCPIVDTWWQ-TETGGNM-MSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVI---------LDSW 470
Cdd:PRK07656 305 LG---VDIVLTGYGlSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVrgpnvmkgyYDDP 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 471 PGQARTLYGDhdrfvdtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:PRK07656 382 EATAAAIDAD-------------GWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAAVIGV 448
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 551 PHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07656 449 PDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
109-547 |
7.39e-44 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 162.43 E-value: 7.39e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQD-VHRGDVVTIYMPMIPEAVVAMLACARIGAIHsVVFGGFSPEALAGRII-DCKSKVVITA 186
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGgVGPGDRVAVLLERSAELVVAILAVLKAGAAY-VPLDPAYPAERLAFILeDAGARLLLTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 187 DEG-IRAGKKIPLKANVDDALTNPETSSiqkvivckrtngaikwnqhrdiwyedlmkVAGAVCAPKEMGAEEAlFILYTS 265
Cdd:TIGR01733 80 SALaSRLAGLVLPVILLDPLELAALDDA-----------------------------PAPPPPDAPSGPDDLA-YVIYTS 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTA----DVGwvtghTYIVYGPLANGATTLLFEGVPNYPDITR 341
Cdd:TIGR01733 130 GSTGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDRVLQFAslsfDAS-----VEEIFGALLAGATLVVPPEDEERDDAAL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 342 VAKIVDKHKVNILYTAPTAIRAMMASGTAACegadgSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpIVDTWWQTE-TGG 420
Cdd:TIGR01733 204 LAALIAEHPVTVLNLTPSLLALLAAALPPAL-----ASLRLVILGGEALTPALVDRWRARGPGAR--LINLYGPTEtTVW 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 421 NMMSPLPGAHALKPGSAA--RPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDHD----RFV-DTYFKTFR 493
Cdd:TIGR01733 277 STATLVDPDDAPRESPVPigRPLANTRLYVLDDDLRPVPVGVVGELYI--GGPGVARGYLNRPEltaeRFVpDPFAGGDG 354
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 494 G-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV 547
Cdd:TIGR01733 355 ArLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
84-616 |
6.83e-42 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 158.66 E-value: 6.83e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVF 163
Cdd:cd17651 3 RQAARTPDAPALVAEG------RRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 164 GGFSPEALAGRIIDCKSKVVITADEgiragkkiplkanVDDALTNPEtssiqkvivckrtngaikwnqhRDIWYEDLMKV 243
Cdd:cd17651 77 PAYPAERLAFMLADAGPVLVLTHPA-------------LAGELAVEL----------------------VAVTLLDQPGA 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 244 AGAVCAPK--EMGAEEALFILYTSGSTGKPKGV--QHTTGGYLLYAaltHERVFDYRPGEIYWCTADVGWVTGhTYIVYG 319
Cdd:cd17651 122 AAGADAEPdpALDADDLAYVIYTSGSTGRPKGVvmPHRSLANLVAW---QARASSLGPGARTLQFAGLGFDVS-VQEIFS 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 320 PLANGATTLLfegVPNY--PDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINPEAwDW 397
Cdd:cd17651 198 TLCAGATLVL---PPEEvrTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPL--GVRLAALRYLLTGGEQLVLTE-DL 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGQSRCPIVDTWWQTET---GGNMMSPLPGAHALKPgSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQA 474
Cdd:cd17651 272 REFCAGLPGLRLHNHYGPTEThvvTALSLPGDPAAWPAPP-PIGRPIDNTRVYVLDAALRPVPPGVPGELYI--GGAGLA 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 475 RTLYGD----HDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:cd17651 349 RGYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLAR 428
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 551 PHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd17651 429 EDRPGEKRLVAYVVGDPEAPVDAA---ELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
106-617 |
2.18e-41 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 157.64 E-value: 2.18e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:TIGR03098 24 RTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGVFVPINPLLKAEQVAHILADCNVRLLVT 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEGIRAgkkiplkanVDDALtnPETSSIQKVI-VCKRTNGAIKWNQHRDIWYEDLMKVAGAV----CAPKEMGAeealf 260
Cdd:TIGR03098 104 SSERLDL---------LHPAL--PGCHDLRTLIiVGDPAHASEGHPGEEPASWPKLLALGDADpphpVIDSDMAA----- 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGV--QHTTggyLLYAALTHERVFDYRPGEiywctadvgwvtghTYIVYGPLA-----NGATTLLFEG- 332
Cdd:TIGR03098 168 ILYTSGSTGRPKGVvlSHRN---LVAGAQSVATYLENRPDD--------------RLLAVLPLSfdygfNQLTTAFYVGa 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 --VP-NYPDITRVAKIVDKHKVNILYTAPtAIRAMMASgtAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpI 409
Cdd:TIGR03098 231 tvVLhDYLLPRDVLKALEKHGITGLAAVP-PLWAQLAQ--LDWPESAAPSLRYLTNSGGAMPRATLSRLRSFLPNAR--L 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 410 VDTWWQTETGGNMMSPlPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVilDSWPGQARTLYGDHDRFVDTYF 489
Cdd:TIGR03098 306 FLMYGLTEAFRSTYLP-PEEVDRRPDSIGKAIPNAEVLVLREDGSECAPGEEGELV--HRGALVAMGYWNDPEKTAERFR 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 490 KT--FRG-------MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIY 560
Cdd:TIGR03098 383 PLppFPGelhlpelAVWSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAVAFGVPDPTLGQAIV 462
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 561 VYVTLNGGEE-PTEALRLELknwvRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:TIGR03098 463 LVVTPPGGEElDRAALLAEC----RARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
80-617 |
9.63e-41 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 156.48 E-value: 9.63e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 80 NCLDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIH 159
Cdd:PRK07786 21 NQLARHALMQPDAPALRFLG------NTTTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIA 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 160 SVVFGGFSPEALAGRIIDCKSKVVITadEGIRAgkkiPLKANVDDALTNPETSsiqkVIVCKRTNGAIkwnqhrdIWYED 239
Cdd:PRK07786 95 VPVNFRLTPPEIAFLVSDCGAHVVVT--EAALA----PVATAVRDIVPLLSTV----VVAGGSSDDSV-------LGYED 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 240 LMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKG--VQHTTggyLLYAALTHERVFDYRPGEiywctaDVGWVTGHTYIV 317
Cdd:PRK07786 158 LLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGavLTHAN---LTGQAMTCLRTNGADINS------DVGFVGVPLFHI 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGpLANGATTLLFeGVPN--YP----DITRVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADgSSLRLLGSVGEPIN 391
Cdd:PRK07786 229 AG-IGSMLPGLLL-GAPTviYPlgafDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQA--RPRD-LALRVLSWGAAPAS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 392 PEAWDWYYKNVGQSRcpIVDTWWQTEtggnmMSP----LPGAHAL-KPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVI 466
Cdd:PRK07786 304 DTLLRQMAATFPEAQ--ILAAFGQTE-----MSPvtcmLLGEDAIrKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVY 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 467 ldswpgQARTLYGDHDRFVDTYFKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEA 545
Cdd:PRK07786 377 ------RAPTLMSGYWNNPEATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEV 450
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 734849183 546 AVVGVPHDIKGQGIYVYVTLNGGeepTEALRL-ELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07786 451 AVIGRADEKWGEVPVAVAAVRND---DAALTLeDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
87-615 |
3.41e-40 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 154.73 E-value: 3.41e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 87 AER-GDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFG 164
Cdd:PRK08314 20 ARRyPDKTAIVFYG------RAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 165 GFSPEALAGRIIDCKSKVVIT----ADEGIRAGKKIPLK----ANVDDALTN------PETSSIQKVIVCKRTNGAIKWN 230
Cdd:PRK08314 94 MNREEELAHYVTDSGARVAIVgselAPKVAPAVGNLRLRhvivAQYSDYLPAepeiavPAWLRAEPPLQALAPGGVVAWK 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 231 QHRDiwyedlmkvAGAVCAPKEMGAEEALFILYTSGSTGKPKG-------VQHTTGGYLLYAALTHERVFdyrpgeiyWC 303
Cdd:PRK08314 174 EALA---------AGLAPPPHTAGPDDLAVLPYTSGTTGVPKGcmhthrtVMANAVGSVLWSNSTPESVV--------LA 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 304 TADVGWVTGHTYIVYGPLANGATTLLfegVPNYpDITRVAKIVDKHKVnilyTAPTAIRAMMAS--GTAACEGADGSSLR 381
Cdd:PRK08314 237 VLPLFHVTGMVHSMNAPIYAGATVVL---MPRW-DREAAARLIERYRV----THWTNIPTMVVDflASPGLAERDLSSLR 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 382 LLGSVGEPInPEAwdwyyknVGQ---SRC--PIVDTWWQTETggnmMSPL---PGAHAlKPGSAARPFFGVvPALVDNLG 453
Cdd:PRK08314 309 YIGGGGAAM-PEA-------VAErlkELTglDYVEGYGLTET----MAQThsnPPDRP-KLQCLGIPTFGV-DARVIDPE 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 454 NLIEGPA--EGNLVIldSWPGQARTLYGDHDRFVDTyFKTFRGMYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRMGT 529
Cdd:PRK08314 375 TLEELPPgeVGEIVV--HGPQVFKGYWNRPEATAEA-FIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWP 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 530 AEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:PRK08314 452 AEVENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPEARGKTTEE-EIIAWAREHMAAYKYPRIVEFVDSLPKSGSGK 530
|
....*.
gi 734849183 610 IMRRIL 615
Cdd:PRK08314 531 ILWRQL 536
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
82-622 |
7.18e-40 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 154.06 E-value: 7.18e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK13295 30 LDACVASCPDKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNP 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITAdegiRAGKKIPLKANVddALTNPETSSIQKVIVCKrTNGAIKWNQHRDIWYEDLM 241
Cdd:PRK13295 110 LMPIFRERELSFMLKHAESKVLVVP----KTFRGFDHAAMA--RRLRPELPALRHVVVVG-GDGADSFEALLITPAWEQE 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTT----GGYLLYAalthERVfDYRPGEIYWCTADVGWVTGHTYIV 317
Cdd:PRK13295 183 PDAPAILARLRPGPDDVTQLIYTSGTTGEPKGVMHTAntlmANIVPYA----ERL-GLGADDVILMASPMAHQTGFMYGL 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGPLANGATTLLFEgvpnYPDITRVAKIVDKHKVNilYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDW 397
Cdd:PRK13295 258 MMPVMLGATAVLQD----IWDPARAAELIRTEGVT--FTMASTPFLTDLTRAVKESGRPVSSLRTFLCAGAPIPGALVER 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGQSrcpIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldswpgQARTL 477
Cdd:PRK13295 332 ARAALGAK---IVSAWGMTENGAVTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQV------RGCSN 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 478 YGDHDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ 557
Cdd:PRK13295 403 FGGYLKRPQLNGTDADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGE 482
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 558 GIYVYVTLNGGEEPTEAlrlELKNWVR-KEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATGE 622
Cdd:PRK13295 483 RACAFVVPRPGQSLDFE---EMVEFLKaQKVAKQYIPERLVVRDALPRTPSGKIQKFRLREMLRGE 545
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
82-615 |
2.28e-38 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 148.58 E-value: 2.28e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:cd17646 4 VAEQAARTPDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVIT-ADEGIRAGKKIPLKANVDDALTNPETSSIQKVIvckrtngaikwnqhrdiwyedl 240
Cdd:cd17646 78 LDPGYPADRLAYMLADAGPAVVLTtADLAARLPAGGDVALLGDEALAAPPATPPLVPP---------------------- 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 mkvagavcapkemGAEEALFILYTSGSTGKPKGVQHTTGG---YLL-----YAALTHERV-------FDYRPGEIYWcta 305
Cdd:cd17646 136 -------------RPDNLAYVIYTSGSTGRPKGVMVTHAGivnRLLwmqdeYPLGPGDRVlqktplsFDVSVWELFW--- 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 306 dvgwvtghtyivygPLANGATTLLFEgVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACegadGSSLRLLGS 385
Cdd:cd17646 200 --------------PLVAGARLVVAR-PGGHRDPAYLAALIREHGVTTCHFVPSMLRVFLAEPAAGS----CASLRRVFC 260
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 386 VGEPINPEAWDWYYKnvgQSRCPIVDTWWQTETGGNMMS-PLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNL 464
Cdd:cd17646 261 SGEALPPELAARFLA---LPGAELHNLYGPTEAAIDVTHwPVRGPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGEL 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 465 VIldSWPGQARTLYG----DHDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHP 540
Cdd:cd17646 338 YL--GGVQLARGYLGrpalTAERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHP 415
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 541 KVAEAAVVGVPHDIKGQGIYVYVTLNGGEEP--TEALRLELKNWVRkeigPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17646 416 AVTHAVVVARAAPAGAARLVGYVVPAAGAAGpdTAALRAHLAERLP----EYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
91-615 |
2.38e-38 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 147.84 E-value: 2.38e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAihsvVFGGFSPEA 170
Cdd:cd17643 2 EAVAVVDED------RRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGG----AYVPIDPAY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIdckskvVITADEGIRAgkkiplkanvddALTNPEtssiqkvivckrtngaikwnqhrdiwyedlmkvagavcap 250
Cdd:cd17643 72 PVERIA------FILADSGPSL------------LLTDPD---------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 251 kemgaeEALFILYTSGSTGKPKGVQ--HTTGGYLLYAAlthERVFDYRPGeiywctaDVgWVTGHTYI-------VYGPL 321
Cdd:cd17643 94 ------DLAYVIYTSGSTGRPKGVVvsHANVLALFAAT---QRWFGFNED-------DV-WTLFHSYAfdfsvweIWGAL 156
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ANGATTLLfegVPNypDITR----VAKIVDKHKVNILYTAPTAIRAMMAsgtAACEGADGS-SLRLLGSVGEPINPEAWD 396
Cdd:cd17643 157 LHGGRLVV---VPY--EVARspedFARLLRDEGVTVLNQTPSAFYQLVE---AADRDGRDPlALRYVIFGGEALEAAMLR 228
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 397 WYYKNVGQSRCPIVDTWWQTETG-GNMMSPLPGAHAlkPGSAA----RPFFGVVPALVDNLGNLIEGPAEGNLVIldSWP 471
Cdd:cd17643 229 PWAGRFGLDRPQLVNMYGITETTvHVTFRPLDAADL--PAAAAspigRPLPGLRVYVLDADGRPVPPGVVGELYV--SGA 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 472 GQARTLYG----DHDRFV-DTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAA 546
Cdd:cd17643 305 GVARGYLGrpelTAERFVaNPFGGPGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAA 384
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 547 VVgVPHDIKG-QGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17643 385 VI-VREDEPGdTRLVAYVVADDGAAADIA---ELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
93-616 |
3.84e-38 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 148.13 E-value: 3.84e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 93 AAIIWEgddpaESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALA 172
Cdd:PRK08276 2 AVIMAP-----SGEVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIA 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 173 GRIIDCKSKVVIT----ADEGIRAGKKIPlkANVDDALTNPETSSIqkvivckrtngaikwnqHRDiwYEDLmkVAGAvc 248
Cdd:PRK08276 77 YIVDDSGAKVLIVsaalADTAAELAAELP--AGVPLLLVVAGPVPG-----------------FRS--YEEA--LAAQ-- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 249 aPKEMGAEEAL--FILYTSGSTGKPKGVQhttggyllyAALTHERVfDYRPGEIYW-CTADVGWVTGHTYIVYGPL---- 321
Cdd:PRK08276 132 -PDTPIADETAgaDMLYSSGTTGRPKGIK---------RPLPGLDP-DEAPGMMLAlLGFGMYGGPDSVYLSPAPLyhta 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ---------ANGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINP 392
Cdd:PRK08276 201 plrfgmsalALGGTVVVMEKF----DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAPCPV 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 393 EA----WDWYyknvGqsrcPIVD-TWWQTEtgGNMMSPLPGAHAL-KPGSAARPFFGVVPALVDNlGNliEGPAEGNLVI 466
Cdd:PRK08276 277 EVkramIDWW----G----PIIHeYYASSE--GGGVTVITSEDWLaHPGSVGKAVLGEVRILDED-GN--ELPPGEIGTV 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 467 LDSWPGQARTLYGDHDRFVDTYFKtfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAA 546
Cdd:PRK08276 344 YFEMDGYPFEYHNDPEKTAAARNP--HGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVA 421
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 547 VVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK08276 422 VFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
104-615 |
2.16e-37 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 145.08 E-value: 2.16e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHsvvfggfspealagriidckskvv 183
Cdd:cd05945 13 GGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAY------------------------ 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 itadegiragkkIPLkanvdDALTNPEtsSIQKVIvckrtngaikwnqhrdiwyeDLMKVAGAVCAPKEMGAeealfILY 263
Cdd:cd05945 69 ------------VPL-----DASSPAE--RIREIL--------------------DAAKPALLIADGDDNAY-----IIF 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPGEIYWCTAD-----------VGWVTGHTYIVYGPLANGATTLLFEG 332
Cdd:cd05945 105 TSGSTGRPKGVQISHDNLVSFTNWMLSD-FPLGPGDVFLNQAPfsfdlsvmdlyPALASGATLVPVPRDATADPKQLFRF 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 VPnypditrvakivdKHKVNILYTAPTAIRAMMASGTAACEGADgsSLRLLGSVGEPI-NPEAWDWyyknvgQSR---CP 408
Cdd:cd05945 184 LA-------------EHGITVWVSTPSFAAMCLLSPTFTPESLP--SLRHFLFCGEVLpHKTARAL------QQRfpdAR 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 409 IVDTWWQTET----GGNMMSPLPgAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILdswpGQARTL-Y----G 479
Cdd:cd05945 243 IYNTYGPTEAtvavTYIEVTPEV-LDGYDRLPIGYAKPGAKLVILDEDGRPVPPGEKGELVIS----GPSVSKgYlnnpE 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 480 DHDRFVDTYFKtfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGI 559
Cdd:cd05945 318 KTAAAFFPDEG--QRAYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPKYKGEKVTEL 395
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 560 YVYVTLNGGEEPteALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05945 396 IAFVVPKPGAEA--GLTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
84-616 |
2.23e-36 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 143.20 E-value: 2.23e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAM----LACARIGAIH 159
Cdd:PRK06188 20 SALKRYPDRPALVLGD------TRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIgaaqLAGLRRTALH 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 160 SVVfggfSPEALAGRIIDCKSKVVItADEGIRAGKKIPLKANVDdaltnpetsSIQKVIVCKRTNGAikwnqhRDIWYE- 238
Cdd:PRK06188 94 PLG----SLDDHAYVLEDAGISTLI-VDPAPFVERALALLARVP---------SLKHVLTLGPVPDG------VDLLAAa 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAPkemGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAAlthervfdyrpgeiyWCTADVGWVTGHTYIVY 318
Cdd:PRK06188 154 AKFGPAPLVAAA---LPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ---------------IQLAEWEWPADPRFLMC 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 319 GPLANGATTL----LFEG-----VPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGtaACEGADGSSLRLLGSVGEP 389
Cdd:PRK06188 216 TPLSHAGGAFflptLLRGgtvivLAKF-DPAEVLRAIEEQRITATFLVPTMIYALLDHP--DLRTRDLSSLETVYYGASP 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 390 INP----EAWD-------WYYknvGQSRCPIVDTWWQTETGgnmmsplPGAHALKPGSAARPFFGVVPALVDNLGNLIEG 458
Cdd:PRK06188 293 MSPvrlaEAIErfgpifaQYY---GQTEAPMVITYLRKRDH-------DPDDPKRLTSCGRPTPGLRVALLDEDGREVAQ 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 459 --PAE----GNLVILDSWPGQARTLygdhdrfvdtyfKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAE 531
Cdd:PRK06188 363 geVGEicvrGPLVMDGYWNRPEETA------------EAFRDGWLhTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPRE 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 532 IESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:PRK06188 431 VEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTALGKPD 507
|
....*
gi 734849183 612 RRILR 616
Cdd:PRK06188 508 KKALR 512
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
82-620 |
2.80e-36 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 143.36 E-value: 2.80e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIwegddpAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsV 161
Cdd:COG1021 31 LRRRAERHPDRIAVV------DGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI--P 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSP-----EALAGRIidcKSKVVITADEgiragkkIPLKANVD--DALTNpETSSIQKVIVCKRTNGAIKWNQhrd 234
Cdd:COG1021 103 VFALPAHrraeiSHFAEQS---EAVAYIIPDR-------HRGFDYRAlaRELQA-EVPSLRHVLVVGDAGEFTSLDA--- 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 235 iWYEDlmKVAGAVCAPkemGAEEALFILYTSGSTGKPKGVQHTTGGYLlYAALTHERVFDYRPGEIYWCTADVGwvtgHT 314
Cdd:COG1021 169 -LLAA--PADLSEPRP---DPDDVAFFQLSGGTTGLPKLIPRTHDDYL-YSVRASAEICGLDADTVYLAALPAA----HN 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 315 YI-----VYGPLANGATTLLfegVPNyPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGsvgep 389
Cdd:COG1021 238 FPlsspgVLGVLYAGGTVVL---APD-PSPDTAFPLIERERVTVTALVPPLALLWLDA--AERSRYDLSSLRVLQ----- 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 390 inpeawdwyyknVGqsrcpivdtwwqtetggnmmsplpGAHaLKPGSAARpffgVVPALVDNLGNLIeGPAEG--NLVIL 467
Cdd:COG1021 307 ------------VG------------------------GAK-LSPELARR----VRPALGCTLQQVF-GMAEGlvNYTRL 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 DSWPGQARTLYG----DHD--RFVDT------------------YfkTFRGMY---------FTGDG-------ARRDED 507
Cdd:COG1021 345 DDPEEVILTTQGrpisPDDevRIVDEdgnpvppgevgelltrgpY--TIRGYYrapehnaraFTPDGfyrtgdlVRRTPD 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 508 GYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNgGEEPTealRLELKNWVRkEI 587
Cdd:COG1021 423 GYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLT---LAELRRFLR-ER 497
|
570 580 590
....*....|....*....|....*....|....*.
gi 734849183 588 GpIAS---PDVIQWAPGLPKTRSGKIMRRILRKIAT 620
Cdd:COG1021 498 G-LAAfklPDRLEFVDALPLTAVGKIDKKALRAALA 532
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
82-617 |
3.01e-36 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 142.77 E-value: 3.01e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDDPAEsRTiTYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:cd12119 2 LEHAARLHGDREIVSRTHEGEVH-RY-TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITaDEGIragkkIPLKANVDDALTNpetssIQKVIVCKRTNGAIKWNQHRDIWYEDLM 241
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFV-DRDF-----LPLLEAIAPRLPT-----VEHVVVMTDDAAMPEPAGVGVLAYEELL 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYA-ALTHERVFDYRPGEIY-----------WCTADVGW 309
Cdd:cd12119 149 AAESPEYDWPDFDENTAAAICYTSGTTGNPKGVVYSHRSLVLHAmAALLTDGLGLSESDVVlpvvpmfhvnaWGLPYAAA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 310 VTGHTYIVYGPLANGATtllfegvpnypditrVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADGSSLRLL---GSV 386
Cdd:cd12119 229 MVGAKLVLPGPYLDPAS---------------LAELIEREGVTFAAGVPTVWQGLLDHLEA--NGRDLSSLRRVvigGSA 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 387 GEPINPEAWDWYYKNVGQSrcpivdtWWQTETG--GNMMSPLPGAHALKPGSAA-------RPFFGVVPALVDNLGNLIE 457
Cdd:cd12119 292 VPRSLIEAFEERGVRVIHA-------WGMTETSplGTVARPPSEHSNLSEDEQLalrakqgRPVPGVELRIVDDDGRELP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 458 --GPAEGNLVILDSWpgQARTLYGDHDR----FVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAE 531
Cdd:cd12119 365 wdGKAVGELQVRGPW--VTKSYYKNDEEsealTEDGWLRT-------GDVATIDEDGYLTITDRSKDVIKSGGEWISSVE 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 532 IESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:cd12119 436 LENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTAE---ELLEFLADKVAKWWLPDDVVFVDEIPKTSTGKID 512
|
....*.
gi 734849183 612 RRILRK 617
Cdd:cd12119 513 KKALRE 518
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
263-620 |
3.79e-36 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 139.15 E-value: 3.79e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATtLLFEGVPNYPDITRV 342
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAH-VVLAGPAGYRNPGLF 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 343 A---KIVDKHKVNILYTAPTAIRAMMASGTaaceGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTE-T 418
Cdd:cd05944 87 DnfwKLVERYRITSLSTVPTVYAALLQVPV----NADISSLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEaT 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 419 GGNMMSPLPGAhaLKPGSA--ARPFFGVVPALVDNLGNLIE--GPAEGNLVILDSWPGQARTLYGDHDRFVDTYFKTFRg 494
Cdd:cd05944 160 CLVAVNPPDGP--KRPGSVglRLPYARVRIKVLDGVGRLLRdcAPDEVGEICVAGPGVFGGYLYTEGNKNAFVADGWLN- 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 495 myfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEA 574
Cdd:cd05944 237 ---TGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVEEE 313
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 734849183 575 lrlELKNWVRKEIGPIAS-PDVIQWAPGLPKTRSGKIMRRILRKIAT 620
Cdd:cd05944 314 ---ELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALRADAI 357
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
261-617 |
5.51e-36 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 140.89 E-value: 5.51e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGVQHTTGGylLYA---ALTHErvfdYRpgeiyWCTADV-----------GWVTGhtyiVYGPLANGAT 326
Cdd:cd05941 94 ILYTSGTTGRPKGVVLTHAN--LAAnvrALVDA----WR-----WTEDDVllhvlplhhvhGLVNA----LLCPLFAGAS 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 tllFEGVPNYpDITRVAKIVDKHKVNILYTAPT-------AIRAMMASGTAACEGADgSSLRLLGSVGEPINPEAWDWYY 399
Cdd:cd05941 159 ---VEFLPKF-DPKEVAISRLMPSITVFMGVPTiytrllqYYEAHFTDPQFARAAAA-ERLRLMVSGSAALPVPTLEEWE 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 400 KNVGQsrcPIVDTWWQTETGGNMMSPLPGAHalKPGSAARPFFGVVPALVDNLGN-----------LIEGPAegnlVILD 468
Cdd:cd05941 234 AITGH---TLLERYGMTEIGMALSNPLDGER--RPGTVGMPLPGVQARIVDEETGeplprgevgeiQVRGPS----VFKE 304
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 469 SWPGQARTlygdHDRFV-DTYFKTfrgmyftGDGARRDEDGYYWITGRV-DDVLNVSGHRMGTAEIESAMVAHPKVAEAA 546
Cdd:cd05941 305 YWNKPEAT----KEEFTdDGWFKT-------GDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECA 373
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 734849183 547 VVGVPHDIKGQGIYVYVTLNGGEEPTEAlrLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05941 374 VIGVPDPDWGERVVAVVVLRAGAAALSL--EELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
82-609 |
1.81e-35 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 143.84 E-value: 1.81e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAihsv 161
Cdd:COG1020 482 FEAQAARTPDAVAVVFGD------QSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGA---- 551
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 vfgGFSP-------EALAGRIIDCKSKVVITAdegiragkkiplkANVDDALTNPETssiqkvivckrtngaikwnqhRD 234
Cdd:COG1020 552 ---AYVPldpaypaERLAYMLEDAGARLVLTQ-------------SALAARLPELGV---------------------PV 594
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 235 IWYEDLMKVAGAVCAPKEMGAEEAL-FILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGE-IYWCTA---DVGw 309
Cdd:COG1020 595 LALDALALAAEPATNPPVPVTPDDLaYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDrVLQFASlsfDAS- 672
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 310 vtghTYIVYGPLANGATTLLF-EGVPnyPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACegadgSSLRLLGSVGE 388
Cdd:COG1020 673 ----VWEIFGALLSGATLVLApPEAR--RDPAALAELLARHRVTVLNLTPSLLRALLDAAPEAL-----PSLRLVLVGGE 741
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 389 PINPEAWDWYYKNVGQSR---------CPIVDTWWQTETGGNMMSPLP-GahalkpgsaaRPffgvvpalVDNLGnlieg 458
Cdd:COG1020 742 ALPPELVRRWRARLPGARlvnlygpteTTVDSTYYEVTPPDADGGSVPiG----------RP--------IANTR----- 798
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 459 paegnLVILDSW--------PGQartLY--GDH-------------DRFVDTYFkTFRG--MYFTGDGARRDEDG---YY 510
Cdd:COG1020 799 -----VYVLDAHlqpvpvgvPGE---LYigGAGlargylnrpeltaERFVADPF-GFPGarLYRTGDLARWLPDGnleFL 869
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 511 witGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKnwvRKEIGPI 590
Cdd:COG1020 870 ---GRADDQVKIRGFRIELGEIEAALLQHPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLAL---ALLLPPY 943
|
570
....*....|....*....
gi 734849183 591 ASPDVIQWAPGLPKTRSGK 609
Cdd:COG1020 944 MVPAAVVLLLPLPLTGNGK 962
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
101-615 |
3.94e-35 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 139.29 E-value: 3.94e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKS 180
Cdd:cd05904 26 DAATGRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 181 KVVITADEGIragKKIPlkanvddaltnpetSSIQKVIVC--KRTNGAIKWnqhRDIWYEDlmkvAGAVCAPkEMGAEEA 258
Cdd:cd05904 106 KLAFTTAELA---EKLA--------------SLALPVVLLdsAEFDSLSFS---DLLFEAD----EAEPPVV-VIKQDDV 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 259 LFILYTSGSTGKPKGVQHTTGGYLLYAALTHERV-FDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLfegVPNYp 337
Cdd:cd05904 161 AALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEgSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVV---MPRF- 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 338 DITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQsrCPIVDTWWQTE 417
Cdd:cd05904 237 DLEELLAAIERYKVTHLPVVPPIVLALVKS--PIVDKYDLSSLRQIMSGAAPLGKELIEAFRAKFPN--VDLGQGYGMTE 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 418 -TGGNMMSPLPGAHALKPGSAARpffgVVPAL----VDNLGNLIEGPAE-GNLVIldswPGQArTLYGdhdrfvdtYFK- 490
Cdd:cd05904 313 sTGVVAMCFAPEKDRAKYGSVGR----LVPNVeakiVDPETGESLPPNQtGELWI----RGPS-IMKG--------YLNn 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 491 --------TFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVY 562
Cdd:cd05904 376 peataatiDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAF 455
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 563 VTLNGGEEPTEAlrlELKNWVRKEIGP------IASPDVIqwapglPKTRSGKIMRRIL 615
Cdd:cd05904 456 VVRKPGSSLTED---EIMDFVAKQVAPykkvrkVAFVDAI------PKSPSGKILRKEL 505
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
106-615 |
6.20e-35 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 139.78 E-value: 6.20e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK06710 48 KDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILC 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 AD------EGIRAGKKIP--LKANVDDALTNPEtSSIQKVIVCKRTNGAIKWNQHRDI--WYEDLMKVAGAVCAPKEMGA 255
Cdd:PRK06710 128 LDlvfprvTNVQSATKIEhvIVTRIADFLPFPK-NLLYPFVQKKQSNLVVKVSESETIhlWNSVEKEVNTGVEVPCDPEN 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALfILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGE-IYWCTADVGWVTGHTYIVYGPLANGATTLLfegVP 334
Cdd:PRK06710 207 DLAL-LQYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEGEeVVLGVLPFFHVYGMTAVMNLSIMQGYKMVL---IP 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 335 NYpDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVG---------QS 405
Cdd:PRK06710 283 KF-DMKMVFEAIKKHKVTLFPGAPTIYIALLNS--PLLKEYDISSIRACISGSAPLPVEVQEKFETVTGgklvegyglTE 359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 406 RCPIVDT--WWQTETGGNMMSPLPGAHA----LKPGSAARPffGVVPALVDNLGNLIEG----PAEGNLVILDSWpgqar 475
Cdd:PRK06710 360 SSPVTHSnfLWEKRVPGSIGVPWPDTEAmimsLETGEALPP--GEIGEIVVKGPQIMKGywnkPEETAAVLQDGW----- 432
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 476 tlygdhdrfvdtyfktfrgmYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIK 555
Cdd:PRK06710 433 --------------------LHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYR 492
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 556 GQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK06710 493 GETVKAFVVLKEGTECSEE---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
83-615 |
7.71e-35 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 138.10 E-value: 7.71e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 83 DRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVV 162
Cdd:cd12117 4 EEQAARTPDAVAVVYGD------RSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 FGGFSPEALAGRIIDCKSKVVITADegiragkkiPLKANVDDALTNPETssiqkvivckrtngaikwnqhrdiwYEDLMK 242
Cdd:cd12117 78 DPELPAERLAFMLADAGAKVLLTDR---------SLAGRAGGLEVAVVI-------------------------DEALDA 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 243 VAGAVCAPKeMGAEEALFILYTSGSTGKPKGVqhttggyllyaALTHERVF------DYR---PGEIYWCTADVGWvTGH 313
Cdd:cd12117 124 GPAGNPAVP-VSPDDLAYVMYTSGSTGRPKGV-----------AVTHRGVVrlvkntNYVtlgPDDRVLQTSPLAF-DAS 190
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 314 TYIVYGPLANGATTLLFE-GVPnyPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGadgssLRLLGSVGEPINP 392
Cdd:cd12117 191 TFEIWGALLNGARLVLAPkGTL--LDPDALGALIAEEGVTVLWLTAALFNQLADEDPECFAG-----LRELLTGGEVVSP 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 393 EAwdwyyknVGQ--SRCP---IVDTWWQTEtggNMMSPLpgAHALKPGSAA-------RPFFGVVPALVDNLGNLIEGPA 460
Cdd:cd12117 264 PH-------VRRvlAACPglrLVNGYGPTE---NTTFTT--SHVVTELDEVagsipigRPIANTRVYVLDEDGRPVPPGV 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 461 EGNLVIldSWPGQARTLYGDHD----RFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAM 536
Cdd:cd12117 332 PGELYV--GGDGLALGYLNRPAltaeRFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAAL 409
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 537 VAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTealrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12117 410 RAHPGVREAVVVVREDAGGDKRLVAYVVAEGALDAA-----ELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
103-615 |
1.63e-34 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 136.68 E-value: 1.63e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd12115 20 CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPERLRFILEDAQARL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 VITadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemGAEEALFIL 262
Cdd:cd12115 100 VLT--------------------------------------------------------------------DPDDLAYVI 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGV--QHTTGGYLLYAALTHervfdyrpgeiywCTADV-GWVTGHTYI--------VYGPLANGATTLLFE 331
Cdd:cd12115 112 YTSGSTGRPKGVaiEHRNAAAFLQWAAAA-------------FSAEElAGVLASTSIcfdlsvfeLFGPLATGGKVVLAD 178
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 332 GVPNYPDITRVAKivdkhkVNILYTAPTAIRAMMAsgtaacEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpIVD 411
Cdd:cd12115 179 NVLALPDLPAAAE------VTLINTVPSAAAELLR------HDALPASVRVVNLAGEPLPRDLVQRLYARLQVER--VVN 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 412 TWWQTE-TGGNMMSPLPgAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGD----HDRFVD 486
Cdd:cd12115 245 LYGPSEdTTYSTVAPVP-PGASGEVSIGRPLANTQAYVLDRALQPVPLGVPGELYI--GGAGVARGYLGRpgltAERFLP 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 487 TYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLN 566
Cdd:cd12115 322 DPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALRSIPGVREAVVVAIGDAAGERRLVAYIVAE 401
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 734849183 567 GGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12115 402 PGAAGLVE---DLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
108-615 |
2.08e-34 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 136.07 E-value: 2.08e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAD 187
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVVGS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EgiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwYEDLMkvagavcapkemgaeealFILYTSGS 267
Cdd:cd05935 82 E------------------------------------------------LDDLA------------------LIPYTSGT 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 268 TGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVpnypDITRVAKIVD 347
Cdd:cd05935 96 TGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARW----DRETALELIE 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 348 KHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSrcpIVDTWWQTETggnmMSP-- 425
Cdd:cd05935 171 KYKVTFWTNIPTMLVDLLA--TPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLR---FVEGYGLTET----MSQth 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPGAHALKPGSAARPFFGVvPALVDNLGNLIEGP--AEGNLVIldSWPgQARTLYGDHDRFVDTYFKTFRGMYF--TGDG 501
Cdd:cd05935 242 TNPPLRPKLQCLGIP*FGV-DARVIDIETGRELPpnEVGEIVV--RGP-QIFKGYWNRPEETEESFIEIKGRRFfrTGDL 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 502 ARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNgGEEPTEALRLELKN 581
Cdd:cd05935 318 GYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLR-PEYRGKVTEEDIIE 396
|
490 500 510
....*....|....*....|....*....|....
gi 734849183 582 WVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05935 397 WAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
101-615 |
2.12e-34 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 137.26 E-value: 2.12e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVfggfSPEALAGRIIDC-- 178
Cdd:cd05923 22 DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALI----NPRLKAAELAELie 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 179 ---KSKVVITAD----EGIRAGKKIPLKANVDDALTNPEtssiqkvivckrtngaikwnqhrdiwyedlmkVAGAVCAPK 251
Cdd:cd05923 98 rgeMTAAVIAVDaqvmDAIFQSGVRVLALSDLVGLGEPE--------------------------------SAGPLIEDP 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 252 EMGAEEALFILYTSGSTGKPKGV---QHTTGGYLLYAAL-------THERVFDYRPgeIYwctadvgwvtgHTYIVYGPL 321
Cdd:cd05923 146 PREPEQPAFVFYTSGTTGLPKGAvipQRAAESRVLFMSTqaglrhgRHNVVLGLMP--LY-----------HVIGFFAVL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 AngaTTLLFEG---VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINpeawDWY 398
Cdd:cd05923 213 V---AALALDGtyvVVEEFDPADALKLIEQERVTSLFATPTHLDALAAAAEFA--GLKLSSLRHVTFAGATMP----DAV 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YKNVGQSR-CPIVDTWWQTETGGNMMSPLPgahalKPGSAARPFFGVVPALVDNLGNLIEGPA---EGNLVILDS----W 470
Cdd:cd05923 284 LERVNQHLpGEKVNIYGTTEAMNSLYMRDA-----RTGTEMRPGFFSEVRIVRIGGSPDEALAngeEGELIVAAAadaaF 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 471 PGQARTLYGDHDRFVDtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:cd05923 359 TGYLNQPEATAKKLQD-------GWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGV 431
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 551 PHDIKGQGIYVYVTLNGGEEPTEALRL-----ELKNWVRkeigpiasPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05923 432 ADERWGQSVTACVVPREGTLSADELDQfcrasELADFKR--------PRRYFFLDELPKNAMNKVLRRQL 493
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
91-616 |
6.76e-34 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 134.80 E-value: 6.76e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEA 170
Cdd:cd17649 2 DAVALVFGD------QSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIDCKSKVVITADegiragkkiplkanvddaltnPETssiqkvivckrtngaikwnqhrdiwyedlmkvagavcap 250
Cdd:cd17649 76 LRYMLEDSGAGLLLTHH---------------------PRQ--------------------------------------- 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 251 kemgaeeALFILYTSGSTGKPKGVQhttggyLLYAALTHE-----RVFDYRPGEIYWCTADVGWVTGHTYiVYGPLANGA 325
Cdd:cd17649 96 -------LAYVIYTSGSTGTPKGVA------VSHGPLAAHcqataERYGLTPGDRELQFASFNFDGAHEQ-LLPPLICGA 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 326 TTLLfEGVPNYPDITRVAKIVDKHKVNILyTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPE-AWDWyyknvGQ 404
Cdd:cd17649 162 CVVL-RPDELWASADELAEMVRELGVTVL-DLPPAYLQQLAEEADRTGDGRPPSLRLYIFGGEALSPElLRRW-----LK 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 405 SRCPIVDTWWQTETggnMMSPL-----PGAHALKPGSA-ARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLY 478
Cdd:cd17649 235 APVRLFNAYGPTEA---TVTPLvwkceAGAARAGASMPiGRPLGGRSAYILDADLNPVPVGVTGELYI--GGEGLARGYL 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 G----DHDRFV-DTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHD 553
Cdd:cd17649 310 GrpelTAERFVpDPFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGA 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 734849183 554 IKGQgIYVYVTLNGGEEPTEaLRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd17649 390 GGKQ-LVAYVVLRAAAAQPE-LRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
104-615 |
8.29e-33 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 132.03 E-value: 8.29e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd12116 9 DDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPALV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITadegiragkkiplkanvDDALTNPetssiqkVIVCKRTNGAIKWNQHRdiwyedlmkvAGAVCAPKEMGAEEAlFILY 263
Cdd:cd12116 89 LT-----------------DDALPDR-------LPAGLPVLLLALAAAAA----------APAAPRTPVSPDDLA-YVIY 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVqHTTGGYLLYAALTHERVFDYRPGEiywctadvGWVTGHTYI-------VYGPLANGATTLLFEGVPNY 336
Cdd:cd12116 134 TSGSTGRPKGV-VVSHRNLVNFLHSMRERLGLGPGD--------RLLAVTTYAfdislleLLLPLLAGARVVIAPRETQR 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 pDITRVAKIVDKHKVNILYTAPTAIRAMMASG-------TAACEG-ADGSSL--RLLGSVGEpinpeAWDWYyknvGqsr 406
Cdd:cd12116 205 -DPEALARLIEAHSITVMQATPATWRMLLDAGwqgraglTALCGGeALPPDLaaRLLSRVGS-----LWNLY----G--- 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 407 cPIVDTWWQTetggnmmsplpgAHALKPGSAA----RPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDHD 482
Cdd:cd12116 272 -PTETTIWST------------AARVTAAAGPipigRPLANTQVYVLDAALRPVPPGVPGELYI--GGDGVAQGYLGRPA 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 483 ----RFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDiKGQ 557
Cdd:cd12116 337 ltaeRFVPDPFAGPGSrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVVREDG-GDR 415
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 558 GIYVYVTLNGGEEP-TEALRLELKN-----WVrkeigpiasPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12116 416 RLVAYVVLKAGAAPdAAALRAHLRAtlpayMV---------PSAFVRLDALPLTANGKLDRKAL 470
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
90-609 |
9.25e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 132.70 E-value: 9.25e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 90 GDQAAIIWeGDdpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPE 169
Cdd:PRK07798 17 PDRVALVC-GD-----RRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVED 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 170 ALAGRIIDCKSKVVITADEgiragkkipLKANVDDALtnPETSSIQKVIVCKrtNGAIKWNQHRDIWYEDLMKVAGAVCA 249
Cdd:PRK07798 91 ELRYLLDDSDAVALVYERE---------FAPRVAEVL--PRLPKLRTLVVVE--DGSGNDLLPGAVDYEDALAAGSPERD 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 250 PKEmGAEEALFILYTSGSTGKPKGV---QHTT-----GGYLLYaalTHERVFDYrpgeiYWCTADVGWVTGHTYIVYGPL 321
Cdd:PRK07798 158 FGE-RSPDDLYLLYTGGTTGMPKGVmwrQEDIfrvllGGRDFA---TGEPIEDE-----EELAKRAAAGPGMRRFPAPPL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 322 ANGATTL-----LFEG--VPNYPDIT----RVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPI 390
Cdd:PRK07798 229 MHGAGQWaafaaLFSGqtVVLLPDVRfdadEVWRTIEREKVNVITIVGDAMARPLLDALEARGPYDLSSLFAIASGGALF 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 391 NP---EAWDWYYKNVgqsrcPIVDTWWQTETGgNMMSplpGAHALKPGSAARPFFGVVP--ALVDNLGNLIEgPAEGNLV 465
Cdd:PRK07798 309 SPsvkEALLELLPNV-----VLTDSIGSSETG-FGGS---GTVAKGAVHTGGPRFTIGPrtVVLDEDGNPVE-PGSGEIG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 466 ILdswpgqART------LYGDHDRFVDTyFKTFRGM-Y-FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMV 537
Cdd:PRK07798 379 WI------ARRghiplgYYKDPEKTAET-FPTIDGVrYaIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALK 451
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 734849183 538 AHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:PRK07798 452 AHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGK 520
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
84-618 |
1.04e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 130.05 E-value: 1.04e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLAERG-DQAAIIwegDDPAesrTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVV 162
Cdd:PRK07788 56 AHAARRApDRAALI---DERG---TLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILL 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 FGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIP--------LKANVDD-ALTNPETSSIQKVIVCKRTNGAIKWNQHr 233
Cdd:PRK07788 130 NTGFSGPQLAEVAAREGVKALVYDDEFTDLLSALPpdlgrlraWGGNPDDdEPSGSTDETLDDLIAGSSTAPLPKPPKP- 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 234 diwyedlmkvagavcapkemgaeeALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVfDYRPGEIYWCTADVGWVTGH 313
Cdd:PRK07788 209 ------------------------GGIVILTSGTTGTPKGAPRPEPSPLAPLAGLLSRV-PFRAGETTLLPAPMFHATGW 263
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 314 TYIVYGpLANGATTLL---FegvpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPI 390
Cdd:PRK07788 264 AHLTLA-MALGSTVVLrrrF-------DPEATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSAL 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 391 NPEAWDWYYKNVGqsrcPIV-DTWWQTETGGNMMSPlPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDS 469
Cdd:PRK07788 336 SPELATRALEAFG----PVLyNLYGSTEVAFATIAT-PEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNG 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 470 WPGQARTLYGDHDRfVDtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:PRK07788 411 FPFEGYTDGRDKQI-ID-------GLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIG 482
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 550 VPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASP-DVIqWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK07788 483 VDDEEFGQRLRAFVVKAPGAALDED---AIKDYVRDNLARYKVPrDVV-FLDELPRNPTGKVLKRELREM 548
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
83-616 |
1.60e-31 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 129.04 E-value: 1.60e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 83 DRHLAERGDQAAIIWEGDDpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVV 162
Cdd:PRK13391 4 GIHAQTTPDKPAVIMASTG----EVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 FGGFSPEALAGRIIDCKSKVVIT----ADEGIRAGKKIP-----LKANVDDALTNpetssiqkvivckrtngaikwnqhr 233
Cdd:PRK13391 80 NSHLTPAEAAYIVDDSGARALITsaakLDVARALLKQCPgvrhrLVLDGDGELEG------------------------- 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 234 diwYEDLMKVAGAVcaPKEMGAEEAL--FILYTSGSTGKPKGV---------QHTTGgyllYAALTHeRVFDYRPGEIYW 302
Cdd:PRK13391 135 ---FVGYAEAVAGL--PATPIADESLgtDMLYSSGTTGRPKGIkrplpeqppDTPLP----LTAFLQ-RLWGFRSDMVYL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 303 CTADVGwvtgHTyivyGPLA-------NGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGA 375
Cdd:PRK13391 205 SPAPLY----HS----APQRavmlvirLGGTVIVMEHF----DAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKY 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 376 DGSSLRLLGSVGEPINPEA----WDWYyknvgqsrCPIVDTWWQTeTGGNMMSPLPGAHAL-KPGSAARPFFGVVPALVD 450
Cdd:PRK13391 273 DLSSLEVAIHAAAPCPPQVkeqmIDWW--------GPIIHEYYAA-TEGLGFTACDSEEWLaHPGTVGRAMFGDLHILDD 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 451 NLGNLiegPaegnlvildswPGQARTLYGDHDR-FVdtYF----KTFR------GMYFTGDGARRDEDGYYWITGRVDDV 519
Cdd:PRK13391 344 DGAEL---P-----------PGEPGTIWFEGGRpFE--YLndpaKTAEarhpdgTWSTVGDIGYVDEDGYLYLTDRAAFM 407
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 520 LNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWA 599
Cdd:PRK13391 408 IISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFE 487
|
570
....*....|....*..
gi 734849183 600 PGLPKTRSGKIMRRILR 616
Cdd:PRK13391 488 DELPRLPTGKLYKRLLR 504
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
117-616 |
6.41e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 123.32 E-value: 6.41e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 117 VCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVF----GGFSPEALAGRIIDCKSKVVItADEGIRA 192
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFvplnPTLKESVLRYLVADAGGRIVL-ADAGAAD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 193 GKKIPLKANVDDaltnpetssiqkvivckrtngaikwnqhrDIWY-EDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKP 271
Cdd:cd05922 82 RLRDALPASPDP-----------------------------GTVLdADGIRAARASAPAHEVSHEDLALLLYTSGSTGSP 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 272 KGVQ--HTTggyLLYAALTHERVFDYRPGEIYWCTADVGWVTGhTYIVYGPLANGATTLLFEGvpNYPDITrVAKIVDKH 349
Cdd:cd05922 133 KLVRlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYG-LSVLNTHLLRGATLVLTND--GVLDDA-FWEDLREH 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 350 KVNILYTAPTaIRAMMASGTAACEGAdgSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpIVDTWWQTETGGNMmSPLPGA 429
Cdd:cd05922 206 GATGLAGVPS-TYAMLTRLGFDPAKL--PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATRRM-TYLPPE 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 430 HAL-KPGSAARPffgvVPA---LVDNLGNLIEGPAE-------GNLVILDSWPGQARTLYGDhdrfvdtyfkTFRGMYFT 498
Cdd:cd05922 280 RILeKPGSIGLA----IPGgefEILDDDGTPTPPGEpgeivhrGPNVMKGYWNDPPYRRKEG----------RGGGVLHT 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPhDIKGQGIYVYVTLNGGEEPTEALRLe 578
Cdd:cd05922 346 GDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLP-DPLGEKLALFVTAPDKIDPKDVLRS- 423
|
490 500 510
....*....|....*....|....*....|....*...
gi 734849183 579 lknwVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05922 424 ----LAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
531-609 |
2.29e-29 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 111.10 E-value: 2.29e-29
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 531 EIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVELLEE---ELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
106-549 |
5.72e-29 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 120.39 E-value: 5.72e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05907 4 QPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALFV 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwYEDLMKvagavcapkemgaeealfILYTS 265
Cdd:cd05907 84 ED-------------------------------------------------PDDLAT------------------IIYTS 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVPNYPD------- 338
Cdd:cd05907 97 GTTGRPKGVMLSHRN-ILSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLLDdlsevrp 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 339 --ITRVAKIVDKHKVNILYTAPTAIRAMMAsgtaacEGADGSSLRLLGSVGEPINPEAWDWYYKnVGqsrCPIVDTWWQT 416
Cdd:cd05907 176 tvFLAVPRVWEKVYAAIKVKAVPGLKRKLF------DLAVGGRLRFAASGGAPLPAELLHFFRA-LG---IPVYEGYGLT 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 417 ETGG--NMMSPlpgaHALKPGSAARPFFGVVPALVDNLGNLIEGPAegnlVILDSW-PGQARTLYGDHDrfvdtyfktfr 493
Cdd:cd05907 246 ETSAvvTLNPP----GDNRIGTVGKPLPGVEVRIADDGEILVRGPN----VMLGYYkNPEATAEALDAD----------- 306
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 494 GMYFTGDGARRDEDGYYWITGRVDDVL-NVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:cd05907 307 GWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
82-619 |
7.83e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 121.00 E-value: 7.83e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK06164 16 LDAHARARPDAVALIDED------RPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADegirAGKKIPLKANVDDAlTNPETSSIQKVIVCKRTNGAI------KWNQHRDi 235
Cdd:PRK06164 90 VNTRYRSHEVAHILGRGRARWLVVWP----GFKGIDFAAILAAV-PPDALPPLRAIAVVDDAADATpapapgARVQLFA- 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 236 wYEDLMKVAGAVCAPkemGAEEALFILY-TSGSTGKPKGVQHTTGGYLLYAALThERVFDYRPGEIYWCTADVGWVTGHT 314
Cdd:PRK06164 164 -LPDPAPPAAAGERA---ADPDAGALLFtTSGTTSGPKLVLHRQATLLRHARAI-ARAYGYDPGAVLLAALPFCGVFGFS 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 315 YIVyGPLANGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMAsgtAACEGADGSSLRLLGSVGepINPeA 394
Cdd:PRK06164 239 TLL-GALAGGAPLVCEPVF----DAARTARALRRHRVTHTFGNDEMLRRILD---TAGERADFPSARLFGFAS--FAP-A 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 395 WDWYYKNVGQSRCPIVDTWWQTEtggnmMSPLPGAHALKPGSAARPFFGVVPA-------LVDNL-GNLIEGPAEGNLVI 466
Cdd:PRK06164 308 LGELAALARARGVPLTGLYGSSE-----VQALVALQPATDPVSVRIEGGGRPAspearvrARDPQdGALLPDGESGEIEI 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 467 ldSWPGQARTLYGDHDRFVDTYfkTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAA 546
Cdd:PRK06164 383 --RAPSLMRGYLDNPDATARAL--TDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQ 458
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 547 VVGVPHDikGQGI-YVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSG---KIMRRILRKIA 619
Cdd:PRK06164 459 VVGATRD--GKTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMA 530
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
82-549 |
9.50e-29 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 121.36 E-value: 9.50e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDDpaESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:COG1022 17 LRRRAARFPDRVALREKEDG--IWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADEGIRagkkiplkANVDDALtnPETSSIQKVIVCkrtNGAIKWNQHRDIWYEDLM 241
Cdd:COG1022 95 IYPTSSAEEVAYILNDSGAKVLFVEDQEQL--------DKLLEVR--DELPSLRHIVVL---DPRGLRDDPRLLSLDELL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEM------GAEEALF-ILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIY-----WCtadvgW 309
Cdd:COG1022 162 ALGREVADPAELearraaVKPDDLAtIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA-----H 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 310 VTGHTYIVYGpLANGATTllfegvpNYP-DITRVAKIVDKHKVNILYTAP-------TAIRAMMASGT------------ 369
Cdd:COG1022 236 VFERTVSYYA-LAAGATV-------AFAeSPDTLAEDLREVKPTFMLAVPrvwekvyAGIQAKAEEAGglkrklfrwala 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 370 -------AACEG-------------AD-----------GSSLRLLGSVGEPINPEAWDWYYkNVGqsrCPIVDTWWQTET 418
Cdd:COG1022 308 vgrryarARLAGkspslllrlkhalADklvfsklrealGGRLRFAVSGGAALGPELARFFR-ALG---IPVLEGYGLTET 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 419 GGNMMSPLPGAHalKPGSAARPFFGVVPALVDNlGN-LIEGPA---------EGNlvildswpgqARTLYgdhdrfVDTY 488
Cdd:COG1022 384 SPVITVNRPGDN--RIGTVGPPLPGVEVKIAED-GEiLVRGPNvmkgyyknpEAT----------AEAFD------ADGW 444
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 489 FKtfrgmyfTGDGARRDEDGYYWITGRVDDVLNVSGhrmGT----AEIESAMVAHPKVAEAAVVG 549
Cdd:COG1022 445 LH-------TGDIGELDEDGFLRITGRKKDLIVTSG---GKnvapQPIENALKASPLIEQAVVVG 499
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
80-617 |
1.28e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 120.53 E-value: 1.28e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 80 NCLDRHLAERGDQAAIIWeGDdpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIH 159
Cdd:PRK07470 11 HFLRQAARRFPDRIALVW-GD-----RSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAVW 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 160 SVVFGGFSPEALAGriidckskvvITADEGIRAgkkipLKANVDdaltNPEtssiqKVIVCKRTNGAIKW-------NQH 232
Cdd:PRK07470 85 VPTNFRQTPDEVAY----------LAEASGARA-----MICHAD----FPE-----HAAAVRAASPDLTHvvaiggaRAG 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 233 RDiwYEDLMKV-AGAVCAPKEMGAEEALFILYTSGSTGKPKGvqhttggyllyAALTH--------ERVFDYRPGeiywc 303
Cdd:PRK07470 141 LD--YEALVARhLGARVANAAVDHDDPCWFFFTSGTTGRPKA-----------AVLTHgqmafvitNHLADLMPG----- 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 304 tadvgwVTGH-TYIVYGPL------------ANGATTLLFEGVPNypDITRVAKIVDKHKVNILYTAPTaIRAMMASGTA 370
Cdd:PRK07470 203 ------TTEQdASLVVAPLshgagihqlcqvARGAATVLLPSERF--DPAEVWALVERHRVTNLFTVPT-ILKMLVEHPA 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 371 AcEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSrcpIVDTWWQTETGGNMMSPLPGAHALKPGSAAR------PFFGV 444
Cdd:PRK07470 274 V-DRYDHSSLRYVIYAGAPMYRADQKRALAKLGKV---LVQYFGLGEVTGNITVLPPALHDAEDGPDARigtcgfERTGM 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 445 VPALVDNLGNLIEGPAEGNLVILdswpGQArTLYGDHDRfVDTYFKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:PRK07470 350 EVQIQDDEGRELPPGETGEICVI----GPA-VFAGYYNN-PEANAKAFRDGWFrTGDLGHLDARGFLYITGRASDMYISG 423
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 524 GHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ-GIYVYVtLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGL 602
Cdd:PRK07470 424 GSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEvGVAVCV-ARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWDAL 499
|
570
....*....|....*
gi 734849183 603 PKTRSGKIMRRILRK 617
Cdd:PRK07470 500 PKSGYGKITKKMVRE 514
|
|
| menE |
TIGR01923 |
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, ... |
109-612 |
1.81e-28 |
|
O-succinylbenzoate-CoA ligase; This model represents an enzyme, O-succinylbenzoate-CoA ligase, which is involved in the fourth step of the menaquinone biosynthesis pathway. O-succinylbenzoate-CoA ligase, together with menB - naphtoate synthase, take 2-succinylbenzoate and convert it into 1,4-di-hydroxy-2- naphtoate. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone]
Pssm-ID: 162605 [Multi-domain] Cd Length: 436 Bit Score: 118.71 E-value: 1.81e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADe 188
Cdd:TIGR01923 1 TWQDLDCEAAHLAKALKAQGIRSGSRVALVGQNSIEMVLLLHACLLLGAEIAMLNTRLTENERTNQLEDLDVQLLLTDS- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 giragkkiPLKANVDDALTNPEtssiqkvivckrtngaikwnqhrdIWYEDlmkvAGAVCAPKEMGAEEALFILYTSGST 268
Cdd:TIGR01923 80 --------LLEEKDFQADSLDR------------------------IEAAG----RYETSLSASFNMDQIATLMFTSGTT 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 269 GKPKGVQHTTGGYLLYAALTHERvFDYRPGEIYWCTADVGWVTGHTyIVYGPLANGATTLLFEGvpnypdITRVAKIVDK 348
Cdd:TIGR01923 124 GKPKAVPHTFRNHYASAVGSKEN-LGFTEDDNWLLSLPLYHISGLS-ILFRWLIEGATLRIVDK------FNQLLEMIAN 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 349 HKVNILYTAPTAIRAMMASgTAACEgadgsSLR--LLGsvGEPINPEawdwYYKNVGQSRCPIVDTWWQTETGGNMM-SP 425
Cdd:TIGR01923 196 ERVTHISLVPTQLNRLLDE-GGHNE-----NLRkiLLG--GSAIPAP----LIEEAQQYGLPIYLSYGMTETCSQVTtAT 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPGAHAlkPGSAARPFFGV-VPALVDNLGNLIEGPAEGNLVILDSW-PGQARTLygdhdRFVDTYFKTfrgmyftGDGAR 503
Cdd:TIGR01923 264 PEMLHA--RPDVGRPLAGReIKIKVDNKEGHGEIMVKGANLMKGYLyQGELTPA-----FEQQGWFNT-------GDIGE 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNggEEPTEAlrlELKNWV 583
Cdd:TIGR01923 330 LDGEGFLYVLGRRDDLIISGGENIYPEEIETVLYQHPGIQEAVVVPKPDAEWGQVPVAYIVSE--SDISQA---KLIAYL 404
|
490 500
....*....|....*....|....*....
gi 734849183 584 RKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:TIGR01923 405 TEKLAKYKVPIAFEKLDELPYNASGKILR 433
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
91-616 |
2.69e-28 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 119.48 E-value: 2.69e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIwegddpAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAiHSVVFG-GFSPE 169
Cdd:PRK13382 58 DRPGLI------DELGTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLNtSFAGP 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 170 ALAGRIIDCKSKVVITADEGIragkkiplkANVDDALTN-PETSSIqkvivckrtngaIKWNQHRDIWYEDLMKVAGAVC 248
Cdd:PRK13382 131 ALAEVVTREGVDTVIYDEEFS---------ATVDRALADcPQATRI------------VAWTDEDHDLTVEVLIAAHAGQ 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 249 APkEMGAEEALFILYTSGSTGKPKGVQHT-TGGYLLYAAltherVFDYRPgeiywctadvgWVTGHTYIVYGPL--ANGA 325
Cdd:PRK13382 190 RP-EPTGRKGRVILLTSGTTGTPKGARRSgPGGIGTLKA-----ILDRTP-----------WRAEEPTVIVAPMfhAWGF 252
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 326 TTLLFEGVPNYPDITR-------VAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWY 398
Cdd:PRK13382 253 SQLVLAASLACTIVTRrrfdpeaTLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAF 332
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YKNVGQSrcpIVDTWWQTETGgnMMS-PLPGAHALKPGSAARPFFGVVPALVDNLGNliEGPaegnlvildswPGQARTL 477
Cdd:PRK13382 333 MDQFGDV---IYNNYNATEAG--MIAtATPADLRAAPDTAGRPAEGTEIRILDQDFR--EVP-----------TGEVGTI 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 478 YGDHDRFVDTYF----KTFR-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPH 552
Cdd:PRK13382 395 FVRNDTQFDGYTsgstKDFHdGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDD 474
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 553 DIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK13382 475 EQYGQRLAAFVVLKPGASATPE---TLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
82-617 |
2.72e-28 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 119.72 E-value: 2.72e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsV 161
Cdd:PRK05605 38 YDNAVARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAV--V 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGG--FSPEALAGRIIDCKSKVVI---------------TADEGI-------------RAGKKIPLKA--NVDDALTNP 209
Cdd:PRK05605 110 VEHNplYTAHELEHPFEDHGARVAIvwdkvaptverlrrtTPLETIvsvnmiaampllqRLALRLPIPAlrKARAALTGP 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 210 ETssiqkvivckrtnGAIKWnqhrdiwyEDLMKVA----GAVCAPKEMGAEEALFILYTSGSTGKPKGvqhttggyllyA 285
Cdd:PRK05605 190 AP-------------GTVPW--------ETLVDAAiggdGSDVSHPRPTPDDVALILYTSGTTGKPKG-----------A 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 286 ALTHERVFdyrpgeiywCTADVG--WVTG---------------HTY-----IVYGPLAnGATTLLFegvPNyPDITRVA 343
Cdd:PRK05605 238 QLTHRNLF---------ANAAQGkaWVPGlgdgpervlaalpmfHAYgltlcLTLAVSI-GGELVLL---PA-PDIDLIL 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 344 KIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRllgsvgepinpeawdwyYKNVGQSRCPiVDT--WWQTETGGN 421
Cdd:PRK05605 304 DAMKKHPPTWLPGVPPLYEKIAEA--AEERGVDLSGVR-----------------NAFSGAMALP-VSTveLWEKLTGGL 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 422 MM--------SPL----PGAHALKPGSAARPFFGVVPALVD--NLGNLIEGPAEGNLVIldSWPgqaRTLYGDHDRFVDT 487
Cdd:PRK05605 364 LVegygltetSPIivgnPMSDDRRPGYVGVPFPDTEVRIVDpeDPDETMPDGEEGELLV--RGP---QVFKGYWNRPEET 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 488 YfKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLN 566
Cdd:PRK05605 439 A-KSFLDGWFrTGDVVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLE 517
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|.
gi 734849183 567 GGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK05605 518 PGAALDPE---GLRAYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
89-620 |
4.51e-28 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 118.42 E-value: 4.51e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 89 RGDQAAIIwegddpAESRTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFS 167
Cdd:PRK06839 15 HPDRIAII------TEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 168 PEALAGRIIDCKSKVVITADEGIRAGKKIPLKANVDdaltnpetssiqkvivckrtngaikwnqhRDIWYEDLMKVAGAV 247
Cdd:PRK06839 89 ENELIFQLKDSGTTVLFVEKTFQNMALSMQKVSYVQ-----------------------------RVISITSLKEIEDRK 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 248 CAPKEMGAEEALFIL-YTSGSTGKPKGvqhttggyllyAALTHERVFdyrpgeiyWC----TADVGWVTGHTYIVYGPLA 322
Cdd:PRK06839 140 IDNFVEKNESASFIIcYTSGTTGKPKG-----------AVLTQENMF--------WNalnnTFAIDLTMHDRSIVLLPLF 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 323 N-GATTL-----LFEG----VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLGSVGEPInP 392
Cdd:PRK06839 201 HiGGIGLfafptLFAGgviiVPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINC--SKFETTNLQSVRWFYNGGAPC-P 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 393 EAWDWYYKNVGqsrCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPG 472
Cdd:PRK06839 278 EELMREFIDRG---FLFGQGFGMTETSPTVFMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLI--RGPN 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 473 QARTLYGDHDRFVDTYFKtfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPH 552
Cdd:PRK06839 353 VMKEYWNRPDATEETIQD---GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQH 429
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 553 DIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIAT 620
Cdd:PRK06839 430 VKWGEIPIAFIVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQLK 494
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
104-618 |
5.54e-28 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 117.76 E-value: 5.54e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsVVF--GGFSPEALAGRIIDCKSK 181
Cdd:PRK03640 24 EEKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLlnTRLSREELLWQLDDAEVK 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 182 VVITadegiragkkiplkanvDDALTNPETSSIQkvivckrtngaikwnqhrdIWYEDLMKVAGAVCAPKEM-GAEEALF 260
Cdd:PRK03640 102 CLIT-----------------DDDFEAKLIPGIS-------------------VKFAELMNGPKEEAEIQEEfDLDEVAT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGVQHTTGGYLLYA---ALT---HERvfdyrpgEIYWCTADVGWVTGHTyIVYGPLANGATTLLfegVP 334
Cdd:PRK03640 146 IMYTSGTTGKPKGVIQTYGNHWWSAvgsALNlglTED-------DCWLAAVPIFHISGLS-ILMRSVIYGMRVVL---VE 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 335 NYpDITRVAKIVDKHKVNILYTAPTAIRAMMAS-GTAACEgadgSSLR--LLGsvGEPINPEAwdwyYKNVGQSRCPIVD 411
Cdd:PRK03640 215 KF-DAEKINKLLQTGGVTIISVVSTMLQRLLERlGEGTYP----SSFRcmLLG--GGPAPKPL----LEQCKEKGIPVYQ 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 412 TWWQTETGGNMMSpLPGAHAL-KPGSAARPFFGVVPALVDNL--------GN-LIEGPaegNLVildswPGQARTLYGDH 481
Cdd:PRK03640 284 SYGMTETASQIVT-LSPEDALtKLGSAGKPLFPCELKIEKDGvvvppfeeGEiVVKGP---NVT-----KGYLNREDATR 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 DRFVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYV 561
Cdd:PRK03640 355 ETFQDGWFKT-------GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVVGVPDDKWGQVPVA 427
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 562 YVTLngGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK03640 428 FVVK--SGEVTEE---ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
85-617 |
5.68e-28 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 121.04 E-value: 5.68e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 85 HLAERGDQAAiiweGDDPA---ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK12467 516 QLIEAQARQH----PERPAlvfGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVP 591
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADEGIRagkKIPLKANVDDALTNPEtssiqkvivckrtngaikwnqhrdiwyEDLM 241
Cdd:PRK12467 592 LDPEYPQDRLAYMLDDSGVRLLLTQSHLLA---QLPVPAGLRSLCLDEP---------------------------ADLL 641
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERvfdyrpgeiYWCTADVGWVTGHTY------ 315
Cdd:PRK12467 642 CGYSGHNPEVALDPDNLAYVIYTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADDSMLMVSTFafdlgv 712
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 316 -IVYGPLANGATTLLFEgvpnyPDITR----VAKIVDKHKVNILYTAPTAIRAMMAsgtaACEGADGSSLRLLGSVGEPI 390
Cdd:PRK12467 713 tELFGALASGATLHLLP-----PDCARdaeaFAALMADQGVTVLKIVPSHLQALLQ----ASRVALPRPQRALVCGGEAL 783
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 391 NPEAWD-WYYKNVGqsrCPIVDTWWQTETG-GNMMSPLPGAHALKPGSA-ARPFFGVVPALVDNLGNLIEGPAEGNLVIl 467
Cdd:PRK12467 784 QVDLLArVRALGPG---ARLINHYGPTETTvGVSTYELSDEERDFGNVPiGQPLANLGLYILDHYLNPVPVGVVGELYI- 859
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 dSWPGQARTLYG----DHDRFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKV 542
Cdd:PRK12467 860 -GGAGLARGYHRrpalTAERFVPDPFGADGGrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGV 938
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 543 AEAAVVGVPHDIKGQgIYVYVTLNGGEEPTE--ALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK12467 939 REAVVLAQPGDAGLQ-LVAYLVPAAVADGAEhqATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPK 1014
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
82-615 |
1.04e-27 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 117.04 E-value: 1.04e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIwegDDPaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsV 161
Cdd:cd05920 21 LARSAARHPDRIAVV---DGD---RRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--P 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFggfspealagriidckskvvitadegiragkkiplkanvddALTNPETSSIQKVIVCKRTNGAIKWNQHRDIWYEDLM 241
Cdd:cd05920 93 VL-----------------------------------------ALPSHRRSELSAFCAHAEAVAYIVPDRHAGFDHRALA 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCApkemgaEEALFILyTSGSTGKPKGVQHTTGGYLlYAALTHERVFDYRPGEIYWCTADVGwvtgHTYI----- 316
Cdd:cd05920 132 RELAESIP------EVALFLL-SGGTTGTPKLIPRTHNDYA-YNVRASAEVCGLDQDTVYLAVLPAA----HNFPlacpg 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 317 VYGPLANGATTLLfegVPNyPDITRVAKIVDKHKVNILYTAPTAirAMMASGTAACEGADGSSLRLLGSVGEPINPEAwd 396
Cdd:cd05920 200 VLGTLLAGGRVVL---APD-PSPDAAFPLIEREGVTVTALVPAL--VSLWLDAAASRRADLSSLRLLQVGGARLSPAL-- 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 397 wyYKNVGQSRCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPffgVVP----ALVDNLGNLIEGPAEGNLviLDSWPG 472
Cdd:cd05920 272 --ARRVPPVLGCTLQQVFGMAEGLLNYTRLDDPDEVIIHTQGRP---MSPddeiRVVDEEGNPVPPGEEGEL--LTRGPY 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 473 QARTLYG--DHDRfvdTYFkTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:cd05920 345 TIRGYYRapEHNA---RAF-TPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAM 420
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 551 PHDIKGQGIYVYVTLNGGEEPTEALRLELKNwvrKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05920 421 PDELLGERSCAFVVLRDPPPSAAQLRRFLRE---RGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
82-615 |
1.09e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 120.06 E-value: 1.09e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDDpaesrTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK12316 516 LFEEQVERTPEAPALAFGEE-----TLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVP 590
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADegiRAGKKIPLKANVDD-ALTNPetssiqkvivckrtngaikwnqhrDIWYEdl 240
Cdd:PRK12316 591 LDPEYPAERLAYMLEDSGVQLLLSQS---HLGRKLPLAAGVQVlDLDRP------------------------AAWLE-- 641
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 mkvAGAVCAPK-EMGAEEALFILYTSGSTGKPKGV--QHTTGGYLLYAALT------HERVFDYRPgeiywCTADVGwvt 311
Cdd:PRK12316 642 ---GYSEENPGtELNPENLAYVIYTSGSTGKPKGAgnRHRALSNRLCWMQQayglgvGDTVLQKTP-----FSFDVS--- 710
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 312 ghTYIVYGPLANGATtLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMM-ASGTAACegadgSSLRLLGSVGEPI 390
Cdd:PRK12316 711 --VWEFFWPLMSGAR-LVVAAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLqDEDVASC-----TSLRRIVCSGEAL 782
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 391 NPEAWDWYYKNVGQsrCPIVDTWWQTETGGNMmsplpgAH--ALKPGSAA----RPFFGVVPALVDNLGNLIEGPAEGNL 464
Cdd:PRK12316 783 PADAQEQVFAKLPQ--AGLYNLYGPTEAAIDV------THwtCVEEGGDSvpigRPIANLACYILDANLEPVPVGVLGEL 854
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 465 VILDSwpGQARTLYG----DHDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHP 540
Cdd:PRK12316 855 YLAGR--GLARGYHGrpglTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHP 932
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 541 KVAEAAVVGVphdiKGQGIYVYVTLnggEEPTEALRLELKNWVRKEIgpiasPDVI---QWAP--GLPKTRSGKIMRRIL 615
Cdd:PRK12316 933 WVREAAVLAV----DGKQLVGYVVL---ESEGGDWREALKAHLAASL-----PEYMvpaQWLAleRLPLTPNGKLDRKAL 1000
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
105-616 |
2.82e-27 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 115.95 E-value: 2.82e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 105 SRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVI 184
Cdd:PRK12406 9 DRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLI 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 185 T-AD--EGIRagKKIPLKANVDDALTNPETSSIQKVIVCKRT--NGAIKWNQhrdiWYEDLMKVAG-AVCAPKEMgaeea 258
Cdd:PRK12406 89 AhADllHGLA--SALPAGVTVLSVPTPPEIAAAYRISPALLTppAGAIDWEG----WLAQQEPYDGpPVPQPQSM----- 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 259 lfiLYTSGSTGKPKGVQHT--TGGYLLYAALTHERVFDYRPGEIYWCTadvgwvtghtyivyGPL------ANGATTLLF 330
Cdd:PRK12406 158 ---IYTSGTTGHPKGVRRAapTPEQAAAAEQMRALIYGLKPGIRALLT--------------GPLyhsapnAYGLRAGRL 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 331 EGV----PNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEA----WDWYyknv 402
Cdd:PRK12406 221 GGVlvlqPRF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVkramIEWW---- 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 gqsrCP-IVDTWWQTETGGNMMSPLPGAHAlKPGSAARPFFGVVPALVDNLGNLIEGPAEGNlvILDSWPGQARTLYGDH 481
Cdd:PRK12406 296 ----GPvIYEYYGSTESGAVTFATSEDALS-HPGTVGKAAPGAELRFVDEDGRPLPQGEIGE--IYSRIAGNPDFTYHNK 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 DrfvDTYFKTFRGMYFT-GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIY 560
Cdd:PRK12406 369 P---EKRAEIDRGGFITsGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALM 445
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 561 VYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK12406 446 AVVEPQPGATLDEA---DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
109-619 |
2.95e-27 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 116.41 E-value: 2.95e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADe 188
Cdd:PRK12583 47 TWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWVICAD- 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 GIRA-------GKKIP-LKANVDDALTNPETSSIQKVIVC--KRTNGAIKWnqhrdiwyEDLMKVAGAVCAPK------E 252
Cdd:PRK12583 126 AFKTsdyhamlQELLPgLAEGQPGALACERLPELRGVVSLapAPPPGFLAW--------HELQARGETVSREAlaerqaS 197
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 253 MGAEEALFILYTSGSTGKPKGVQHT-----TGGYLLYAAL---THERVFdyRPGEIYWCTadvgwvtGHTYIVYGPLANG 324
Cdd:PRK12583 198 LDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLgltEHDRLC--VPVPLYHCF-------GMVLANLGCMTVG 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 325 ATtLLFEGVPNYPDITRVAkiVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPINPEAWDwyyKNVGQ 404
Cdd:PRK12583 269 AC-LVYPNEAFDPLATLQA--VEEERCTALYGVPTMFIAELDHPQRG--NFDLSSLRTGIMAGAPCPIEVMR---RVMDE 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 405 SRCP-IVDTWWQTETggnmmSPLPGAHA------LKPGSAARPFFGVVPALVDNLGNLIeGPAE-------GNLVILDSW 470
Cdd:PRK12583 341 MHMAeVQIAYGMTET-----SPVSLQTTaaddleRRVETVGRTQPHLEVKVVDPDGATV-PRGEigelctrGYSVMKGYW 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 471 PGQARTLYG-DHDrfvdtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:PRK12583 415 NNPEATAESiDED-----------GWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFG 483
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 550 VPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK12583 484 VPDEKYGEEIVAWVRLHPGHAASEE---ELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREIS 550
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
107-617 |
2.04e-26 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 112.05 E-value: 2.04e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 107 TITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVvita 186
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAEAVLLNTRLTPNELAFQLKDSDVKL---- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 187 degiragkkiplkanvDDALTnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgaeealfILYTSG 266
Cdd:cd05912 77 ----------------DDIAT-----------------------------------------------------IMYTSG 87
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 267 STGKPKGVQHTTGGYLLYAALTHERVfdyrpGeiywCTADVGW--------VTGHTYIVYGpLANGATTLLFEGVpnypD 338
Cdd:cd05912 88 TTGKPKGVQQTFGNHWWSAIGSALNL-----G----LTEDDNWlcalplfhISGLSILMRS-VIYGMTVYLVDKF----D 153
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 339 ITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEgadgSSLR--LLGsvGEPINPEAwdwyYKNVGQSRCPIVDTWWQT 416
Cdd:cd05912 154 AEQVLHLINSGKVTIISVVPTMLQRLLEILGEGYP----NNLRciLLG--GGPAPKPL----LEQCKEKGIPVYQSYGMT 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 417 ETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLiEGPAEgnlvILDSWPGQARTLYGDHDR----FVDTYFKTf 492
Cdd:cd05912 224 ETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPP-YEVGE----ILLKGPNVTKGYLNRPDAteesFENGWFKT- 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 493 rgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNggEEPT 572
Cdd:cd05912 298 ------GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSE--RPIS 369
|
490 500 510 520
....*....|....*....|....*....|....*....|....*
gi 734849183 573 EAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05912 370 EE---ELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
125-616 |
2.06e-26 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 112.86 E-value: 2.06e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 125 RGQDVHRGDVVTIYMPM--IPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIRAGKKIPLKAnv 202
Cdd:cd05929 13 HQRRLLLLDVYSIALNRnaRAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPRAEACAIIEIKAA-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 203 ddaltnpetssiqkVIVCKR-TNGAIKWNqhrdiwYEDLMKVAGAvcAPKEMGAEEAL--FILYTSGSTGKPKGVQHTTG 279
Cdd:cd05929 91 --------------ALVCGLfTGGGALDG------LEDYEAAEGG--SPETPIEDEAAgwKMLYSGGTTGRPKGIKRGLP 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 280 GYLLYAALthervfdyrpgeIYWCTADVGWVTGHTYIVYGPLANGA-----TTLLFEGV-----PNYpDITRVAKIVDKH 349
Cdd:cd05929 149 GGPPDNDT------------LMAAALGFGPGADSVYLSPAPLYHAApfrwsMTALFMGGtlvlmEKF-DPEEFLRLIERY 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 350 KVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINP---EAW-DWYyknvgqsrCPIVDTWWqTETGGNMMSP 425
Cdd:cd05929 216 RVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAPCPPwvkEQWiDWG--------GPIIWEYY-GGTEGQGLTI 286
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPGAHALK-PGSAARPFFGVVpALVDNLGNliEGPaegnlvildswPGQARTLY---GDHDRFVDTYFKTF-----RGMY 496
Cdd:cd05929 287 INGEEWLThPGSVGRAVLGKV-HILDEDGN--EVP-----------PGEIGEVYfanGPGFEYTNDPEKTAaarneGGWS 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALR 576
Cdd:cd05929 353 TLGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTALA 432
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 734849183 577 LELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05929 433 EELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
90-616 |
2.10e-26 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 113.62 E-value: 2.10e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 90 GDQAAIIWEgDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPE 169
Cdd:PRK08008 21 GHKTALIFE-SSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLRE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 170 ALAGRIIDCKSKVVITADE------GIRAGKKIPLKANVDDALTNPETSsiqkvivckrtnGAIKWNQHRDiwyedlmKV 243
Cdd:PRK08008 100 ESAWILQNSQASLLVTSAQfypmyrQIQQEDATPLRHICLTRVALPADD------------GVSSFTQLKA-------QQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 244 AGAVCAPKEMGAEEALFILYTSGSTGKPKGVQhTTGGYLLYA--------ALTHERVF---------DYRpgeiywCTAD 306
Cdd:PRK08008 161 PATLCYAPPLSTDDTAEILFTSGTTSRPKGVV-ITHYNLRFAgyysawqcALRDDDVYltvmpafhiDCQ------CTAA 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 307 VGWVTGhtyivygplanGATTLLFEgvpNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEgaDGSSLRLLgsv 386
Cdd:PRK08008 234 MAAFSA-----------GATFVLLE---KY-SARAFWGQVCKYRATITECIPMMIRTLMVQPPSAND--RQHCLREV--- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 387 gepinpeawdWYYKNVGQSR---------CPIVDTWWQTETGGNMMSPLPGAHALKPgSAARPFFGVVPALVDNLGNliE 457
Cdd:PRK08008 294 ----------MFYLNLSDQEkdafeerfgVRLLTSYGMTETIVGIIGDRPGDKRRWP-SIGRPGFCYEAEIRDDHNR--P 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 458 GPAEgnlVILDSW----PGqaRTLYGDHDRFVDTYFKTFR--GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAE 531
Cdd:PRK08008 361 LPAG---EIGEICikgvPG--KTIFKEYYLDPKATAKVLEadGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVE 435
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 532 IESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:PRK08008 436 LENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKII 512
|
....*
gi 734849183 612 RRILR 616
Cdd:PRK08008 513 KKNLK 517
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
82-617 |
2.37e-26 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 113.16 E-value: 2.37e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEgddpaeSRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:cd12118 10 LERAAAVYPDRTSIVYG------DRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVItadegiragkkiplkanVDDALTnpetssiqkvivckrtngaikwnqhrdiwYEDLM 241
Cdd:cd12118 84 LNTRLDAEEIAFILRHSEAKVLF-----------------VDREFE-----------------------------YEDLL 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KvAGAVCAPKEMGAEE--ALFILYTSGSTGKPKGVQHT-TGGYLlyAALTHERVFDYRPGEIYWCTADVGWVTGHTYIvY 318
Cdd:cd12118 118 A-EGDPDFEWIPPADEwdPIALNYTSGTTGRPKGVVYHhRGAYL--NALANILEWEMKQHPVYLWTLPMFHCNGWCFP-W 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 319 GPLANGATTLLFEGVpNYPDITRvakIVDKHKVNILYTAPTaIRAMMASgtAACEGADGSSLRLLGSVGEPINPEAwdwY 398
Cdd:cd12118 194 TVAAVGGTNVCLRKV-DAKAIYD---LIEKHKVTHFCGAPT-VLNMLAN--APPSDARPLPHRVHVMTAGAPPPAA---V 263
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YKNVGQSRCPIVDTWWQTETGGnmmsP------LPGAHALKPGSAAR-------PFFGVVPALVDNLGNLIEGPAE---- 461
Cdd:cd12118 264 LAKMEELGFDVTHVYGLTETYG----PatvcawKPEWDELPTEERARlkarqgvRYVGLEEVDVLDPETMKPVPRDgkti 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 462 ------GNLVILDswpgqartlygdhdrfvdtYFK-------TFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRM 527
Cdd:cd12118 340 geivfrGNIVMKG-------------------YLKnpeataeAFRGGWFhSGDLAVIHPDGYIEIKDRSKDIIISGGENI 400
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 528 GTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPgLPKTRS 607
Cdd:cd12118 401 SSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPKTST 476
|
570
....*....|
gi 734849183 608 GKIMRRILRK 617
Cdd:cd12118 477 GKIQKFVLRD 486
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
105-615 |
2.95e-26 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 112.75 E-value: 2.95e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 105 SRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHsVVFGGFSPEALAGRII-DCKSKVV 183
Cdd:cd12114 10 DGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAY-VPVDIDQPAARREAILaDAGARLV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITADEgiragkkiPLKANVDDALTNPETSSIQkvivckrtngaikwnqhrdiwyedlmkVAGAVCAPKEMGAEEALFILY 263
Cdd:cd12114 89 LTDGP--------DAQLDVAVFDVLILDLDAL---------------------------AAPAPPPPVDVAPDDLAYVIF 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYL--------LYAALTHERV-------FDYRpgeiywctadvgwvtghTYIVYGPLANGATtL 328
Cdd:cd12114 134 TSGSTGTPKGVMISHRAALntildinrRFAVGPDDRVlalsslsFDLS-----------------VYDIFGALSAGAT-L 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 329 LFEGVPNYPDITRVAKIVDKHKVNILYTAPtAIRAMMASgTAACEGADGSSLRLLGSVGepinpeawDWyyknVGQSrcp 408
Cdd:cd12114 196 VLPDEARRRDPAHWAELIERHGVTLWNSVP-ALLEMLLD-VLEAAQALLPSLRLVLLSG--------DW----IPLD--- 258
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 409 IVDTWWQTETGGNMMSpLPGA---------HALKPGSAA-------RPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPG 472
Cdd:cd12114 259 LPARLRALAPDARLIS-LGGAteasiwsiyHPIDEVPPDwrsipygRPLANQRYRVLDPRGRDCPDWVPGELWI--GGRG 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 473 QARTLYGD----HDRFVDTyfKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVV 548
Cdd:cd12114 336 VALGYLGDpeltAARFVTH--PDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVV 413
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 549 GVPHDIKGQGIYVYVTLNGGEEPT-EALRLELKnwvrKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12114 414 VLGDPGGKRLAAFVVPDNDGTPIApDALRAFLA----QTLPAYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
24-81 |
3.26e-26 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 101.40 E-value: 3.26e-26
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 24 YKAMYQQSVVNPDGFWREQAKRLDWIKPFTTVKQTSFDDHhvdIKWFADGTLNVSYNC 81
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPFDKVLDGSNGPF---AKWFVGGKLNVCYNC 55
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
109-617 |
7.03e-26 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 112.15 E-value: 7.03e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVI--TA 186
Cdd:PRK06087 51 TYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFapTL 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 187 DEGIRAGKKI-PLKANVD--------DALTnPETSSIQKVIVCKRtngaikwnqhrdiwYEDLmkvAGAVCApkemGAEE 257
Cdd:PRK06087 131 FKQTRPVDLIlPLQNQLPqlqqivgvDKLA-PATSSLSLSQIIAD--------------YEPL---TTAITT----HGDE 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 258 ALFILYTSGSTGKPKGVQhttggyllyaaLTH------ERVFDYRPG----EIYWCTADVGWVTGHTYIVYGPLANGATT 327
Cdd:PRK06087 189 LAAVLFTSGTEGLPKGVM-----------LTHnnilasERAYCARLNltwqDVFMMPAPLGHATGFLHGVTAPFLIGARS 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 328 LLFEGVPnyPDitRVAKIVDKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLGSVGEPInP-----EAWDWYYKnv 402
Cdd:PRK06087 258 VLLDIFT--PD--ACLALLEQQRCTCMLGATPFIYDLLN--LLEKQPADLSALRFFLCGGTTI-PkkvarECQQRGIK-- 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 gqsrcpIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDN------LGNLIEGPAEGNLVI---LDSWPGQ 473
Cdd:PRK06087 329 ------LLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEarktlpPGCEGEEASRGPNVFmgyLDEPELT 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 474 ARTLygDHDrfvdtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHD 553
Cdd:PRK06087 403 ARAL--DEE-----------GWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDE 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 554 IKGQGIYVYVTLnggEEPTEALRLE--LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK06087 470 RLGERSCAYVVL---KAPHHSLTLEevVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
106-615 |
7.53e-26 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 111.02 E-value: 7.53e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAihsvVFGGFSPEALAGRIidckskVVIT 185
Cdd:cd17650 11 RQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGG----AYVPIDPDYPAERL------QYML 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEGiragkkiplkanVDDALTNPEtssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgaeEALFILYTS 265
Cdd:cd17650 81 EDSG------------AKLLLTQPE----------------------------------------------DLAYVIYTS 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGV----QHTTGGYLLYAALTHERVFDYRPGEIYWCTADVgwvtghtyiVYGPLANgatTLLFEG----VPN-- 335
Cdd:cd17650 103 GTTGKPKGVmvehRNVAHAAHAWRREYELDSFPVRLLQMASFSFDV---------FAGDFAR---SLLNGGtlviCPDev 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 336 YPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLgSVGEPINPEAW-DWYYKNVGQsRCPIVDTWW 414
Cdd:cd17650 171 KLDPAALYDLILKSRITLMESTPALIRPVMAY--VYRNGLDLSAMRLL-IVGSDGCKAQDfKTLAARFGQ-GMRIINSYG 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 415 QTETGGNM------MSPLPGAHALKPGsaaRPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDHD----RF 484
Cdd:cd17650 247 VTEATIDStyyeegRDPLGDSANVPIG---RPLPNTAMYVLDERLQPQPVGVAGELYI--GGAGVARGYLNRPEltaeRF 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 485 VDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVgVPHDIKGQ-GIYVYV 563
Cdd:cd17650 322 VENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VREDKGGEaRLCAYV 400
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 734849183 564 TlnggeePTEALRL-ELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17650 401 V------AAATLNTaELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
91-616 |
1.53e-25 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 110.87 E-value: 1.53e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIwegddPAES-RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPe 169
Cdd:PRK13390 12 DRPAVI-----VAETgEQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 170 ALAGRII-DCKSKVVIT--ADEGI--RAGKKIPLKanvddaltnpetssiqkVIVCKRTNGAIKwnqhrdiwYEDLMKVA 244
Cdd:PRK13390 86 PEADYIVgDSGARVLVAsaALDGLaaKVGADLPLR-----------------LSFGGEIDGFGS--------FEAALAGA 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 245 GAVCAPKEMGAeealFILYTSGSTGKPKGVQHTTGGYLLYA-----ALTHERVFDYRPGEIYWCTADVGwvtgHTyivyG 319
Cdd:PRK13390 141 GPRLTEQPCGA----VMLYSSGTTGFPKGIQPDLPGRDVDApgdpiVAIARAFYDISESDIYYSSAPIY----HA----A 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 320 PL-------ANGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEP--- 389
Cdd:PRK13390 209 PLrwcsmvhALGGTVVLAKRF----DAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPcpv 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 390 -INPEAWDWyyknVGqsrcPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVpALVDNLGNliEGPAegnlvild 468
Cdd:PRK13390 285 dVKHAMIDW----LG----PIVYEYYSSTEAHGMTFIDSPDWLAHPGSVGRSVLGDL-HICDDDGN--ELPA-------- 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 469 swpGQARTLYGDHDRFVDTYF----KTFRGMY-------FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMV 537
Cdd:PRK13390 346 ---GRIGTVYFERDRLPFRYLndpeKTAAAQHpahpfwtTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALT 422
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 538 AHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK13390 423 MHPAVHDVAVIGVPDPEMGEQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
84-610 |
1.66e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 111.29 E-value: 1.66e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLA-ERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVV 162
Cdd:PRK06178 40 RAWArERPQRPAIIFYG------HVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPV 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 FGGFSPEALAGRIIDCKSKVVITADE------------GIRAGKKIPLKANVDDALTNPETSSIQ--KVIVckrtNGAIk 228
Cdd:PRK06178 114 SPLFREHELSYELNDAGAEVLLALDQlapvveqvraetSLRHVIVTSLADVLPAEPTLPLPDSLRapRLAA----AGAI- 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 229 wnqhrdiwyeDLMKV--AGAVCAPKEMGAEEALFIL-YTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTA 305
Cdd:PRK06178 189 ----------DLLPAlrACTAPVPLPPPALDALAALnYTGGTTGMPKGCEHTQRDMVYTAAAAYAVAVVGGEDSVFLSFL 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 306 DVGWVTGHTYIVYGPLANGATTLLFegvpNYPDITRVAKIVDKHKVNILY-TAPTAIRAMMASGTAAcegADGSSLRLLG 384
Cdd:PRK06178 259 PEFWIAGENFGLLFPLFSGATLVLL----ARWDAVAFMAAVERYRVTRTVmLVDNAVELMDHPRFAE---YDLSSLRQVR 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 385 SVG--EPINPEawdwYYKNvgqsrcpivdtwWQTETGGNM------MSPLPGAHALKPGSA-------ARPFF-GV-VPA 447
Cdd:PRK06178 332 VVSfvKKLNPD----YRQR------------WRALTGSVLaeaawgMTETHTCDTFTAGFQdddfdllSQPVFvGLpVPG 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 448 ----LVD-NLGNLIEGPAEGNLVIldswpgQARTLYGDHDRFVDTYFKTFRGMYF-TGDGARRDEDGYYWITGRVDDVLN 521
Cdd:PRK06178 396 tefkICDfETGELLPLGAEGEIVV------RTPSLLKGYWNKPEATAEALRDGWLhTGDIGKIDEQGFLHYLGRRKEMLK 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 522 VSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPdVIQWAPG 601
Cdd:PRK06178 470 VNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVP-EIRIVDA 545
|
....*....
gi 734849183 602 LPKTRSGKI 610
Cdd:PRK06178 546 LPMTATGKV 554
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
101-616 |
2.96e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 109.90 E-value: 2.96e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKS 180
Cdd:PRK09088 16 DLALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEP 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 181 KVvITADEGIRAGKKIP-----LKANVDdALTNPETSSIQkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgA 255
Cdd:PRK09088 96 RL-LLGDDAVAAGRTDVedlaaFIASAD-ALEPADTPSIP---------------------------------------P 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGV-------QHTTGGYLLYAALTHERVFdyrpgeiyWCTADVGWVTGHTYIVYGPLANGATTL 328
Cdd:PRK09088 135 ERVSLILFTSGTSGQPKGVmlsernlQQTAHNFGVLGRVDAHSSF--------LCDAPMFHIIGLITSVRPVLAVGGSIL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 329 LFEGVPNYPDITRVA--KIVDKHKVNIlytaptairAMMASGTAACEGADGSSLRLLGSV---GEP-INPEAWDWYYKNV 402
Cdd:PRK09088 207 VSNGFEPKRTLGRLGdpALGITHYFCV---------PQMAQAFRAQPGFDAAALRHLTALftgGAPhAAEDILGWLDDGI 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 gqsrcPIVDTWWQTETGGNM-MSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDH 481
Cdd:PRK09088 278 -----PMVDGFGMSEAGTVFgMSVDCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLL--RGPNLSPGYWRRP 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 DRFVDTYfkTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYV 561
Cdd:PRK09088 351 QATARAF--TGDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYL 428
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 562 YVTlnggeePTEALRLELKNWVRKEIGPIAS---PDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK09088 429 AIV------PADGAPLDLERIRSHLSTRLAKykvPKHLRLVDALPRTASGKLQKARLR 480
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
91-625 |
3.66e-25 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 109.59 E-value: 3.66e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGDDpaesrtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEA 170
Cdd:PRK06145 17 DRAALVYRDQE------ISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADE 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIDCKSKVVITaDEGIRAGKKIPLKANVDDALTNPETSSIQKvivckrtngaikwnqhrdiwyedlmkvAGAVCAP 250
Cdd:PRK06145 91 VAYILGDAGAKLLLV-DEEFDAIVALETPKIVIDAAAQADSRRLAQ---------------------------GGLEIPP 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 251 KEMGAEEALF-ILYTSGSTGKPKGVQHTTGGYllyaaltHERVFDYrpgeiywcTADVGWVTGHTYIVYGPLAN-GATTL 328
Cdd:PRK06145 143 QAAVAPTDLVrLMYTSGTTDRPKGVMHSYGNL-------HWKSIDH--------VIALGLTASERLLVVGPLYHvGAFDL 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 329 -----LFEG----VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADGSSLRLLGSVGEPiNPE----AW 395
Cdd:PRK06145 208 pgiavLWVGgtlrIHREFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDR--DRFDLDSLAWCIGGGEK-TPEsrirDF 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 396 DWYYKNVgqsrcPIVDTWWQTET--GGNMMSPlpGAHALKPGSAARPFFGVVPALVDNLGNLIegPAEGNLVILDSWPGQ 473
Cdd:PRK06145 285 TRVFTRA-----RYIDAYGLTETcsGDTLMEA--GREIEKIGSTGRALAHVEIRIADGAGRWL--PPNMKGEICMRGPKV 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 474 ARTLYGDHDR----FVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:PRK06145 356 TKGYWKDPEKtaeaFYGDWFRS-------GDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIG 428
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 550 VPHDIKGQGIYVYVTLNGGeeptEALRLE-LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKiatgEYDG 625
Cdd:PRK06145 429 VHDDRWGERITAVVVLNPG----ATLTLEaLDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLRD----ELNG 497
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
87-615 |
1.03e-24 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 108.18 E-value: 1.03e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 87 AERG-DQAAIIWEGDdpaesrTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVfgg 165
Cdd:cd17655 7 AEKTpDHTAVVFEDQ------TLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI--- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 166 fSPEALAGRII----DCKSKVVITAdegiraGKKIPLKANVDDA-LTNPETSSIQKVIvckrtngaikwNQHRDIWYEDL 240
Cdd:cd17655 78 -DPDYPEERIQyileDSGADILLTQ------SHLQPPIAFIGLIdLLDEDTIYHEESE-----------NLEPVSKSDDL 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MkvagavcapkemgaeealFILYTSGSTGKPKGVQHTTGG-------------------YLLYAALThervFDYRPGEIY 301
Cdd:cd17655 140 A------------------YVIYTSGSTGKPKGVMIEHRGvvnlvewankviyqgehlrVALFASIS----FDASVTEIF 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 302 wctadVGWVTGHTYIVYGplanGATTLlfegvpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGtaaceGADGSSLR 381
Cdd:cd17655 198 -----ASLLSGNTLYIVR----KETVL---------DGQALTQYIRQNRITIIDLTPAHLKLLDAAD-----DSEGLSLK 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 382 LLGSVGEPINPEAWDWYYKNVGQSrCPIVDTWWQTETGGNMMsplpgAHALKPGSAARPFFGVVPALVDNlgnliegpae 461
Cdd:cd17655 255 HLIVGGEALSTELAKKIIELFGTN-PTITNAYGPTETTVDAS-----IYQYEPETDQQVSVPIGKPLGNT---------- 318
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 462 gNLVILDSW----P-GQARTLY--GD-------------HDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLN 521
Cdd:cd17655 319 -RIYILDQYgrpqPvGVAGELYigGEgvargylnrpeltAEKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVK 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 522 VSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNgGEEPTEALRlelkNWVRKEIGPIASPDVIQWAPG 601
Cdd:cd17655 398 IRGYRIELGEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSE-KELPVAQLR----EFLARELPDYMIPSYFIKLDE 472
|
570
....*....|....
gi 734849183 602 LPKTRSGKIMRRIL 615
Cdd:cd17655 473 IPLTPNGKVDRKAL 486
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
103-615 |
2.31e-24 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 106.18 E-value: 2.31e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd17652 8 FGDETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPAL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 VITAdegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgAEEALFIL 262
Cdd:cd17652 88 LLTT--------------------------------------------------------------------PDNLAYVI 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGVQHTTGGYLLYAAlTHERVFDYRPGEIYWCTADVGWvTGHTYIVYGPLANGATTLLfegVPNYPDIT-- 340
Cdd:cd17652 100 YTSGSTGRPKGVVVTHRGLANLAA-AQIAAFDVGPGSRVLQFASPSF-DASVWELLMALLAGATLVL---APAEELLPge 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 341 RVAKIVDKHKVNILYTAPTAIRAMmasgtaacEGADGSSLRLLGSVGEPINPE-AWDW-----YYKNVGQSRCPIVDTWW 414
Cdd:cd17652 175 PLADLLREHRITHVTLPPAALAAL--------PPDDLPDLRTLVVAGEACPAElVDRWapgrrMINAYGPTETTVCATMA 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 415 QTETGGNMM---SPLPGAHALKPGSAARPffgvVPALVDnlgnliegpaeGNLVIldSWPGQARTlYGDH-----DRFVD 486
Cdd:cd17652 247 GPLPGGGVPpigRPVPGTRVYVLDARLRP----VPPGVP-----------GELYI--AGAGLARG-YLNRpgltaERFVA 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 487 TYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTL 565
Cdd:cd17652 309 DPFGAPGSrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRPGDKRLVAYVVP 388
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|
gi 734849183 566 NGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17652 389 APGAAPTAA---ELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
97-617 |
2.44e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 107.46 E-value: 2.44e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 97 WEGDDPA---ESRTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAihsvVFGGFSP---- 168
Cdd:PRK07867 15 AEDDDRGlyfEDSFTSWREHIRGSAARAAALRARlDPTRPPHVGVLLDNTPEFSLLLGAAALSGI----VPVGLNPtrrg 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 169 EALAGRII--DCKskVVITADegiragKKIPLKANVDDALTNPETSSIQkvivckrtngaikwnqhrdiWYEDLMKVAGA 246
Cdd:PRK07867 91 AALARDIAhaDCQ--LVLTES------AHAELLDGLDPGVRVINVDSPA--------------------WADELAAHRDA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 247 VCAPKEMGAEEALFILYTSGSTGKPKGVQhTTGGYLLYAALTHERVFDYRPGEIYWCTAD--------VGWVTGhtyivy 318
Cdd:PRK07867 143 EPPFRVADPDDLFMLIFTSGTSGDPKAVR-CTHRKVASAGVMLAQRFGLGPDDVCYVSMPlfhsnavmAGWAVA------ 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 319 gpLANGATTLL---FEGVPNYPDITRV----AKIVDKHKVNILYTAP------TAIRAMMASgtaacEGADGSSLRLLGS 385
Cdd:PRK07867 216 --LAAGASIALrrkFSASGFLPDVRRYgatyANYVGKPLSYVLATPErpddadNPLRIVYGN-----EGAPGDIARFARR 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 386 VGepinpeawdwyyknvgqsrCPIVDTWWQTEtGGNMMSPLPGAhalKPGSAARPFFGVV-----------PALVDNLGN 454
Cdd:PRK07867 289 FG-------------------CVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdtgtecpPAEDADGRL 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 455 LIEGPAEGNLVILDSwPGQARTLYGDHD----RFVDtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTA 530
Cdd:PRK07867 346 LNADEAIGELVNTAG-PGGFEGYYNDPEadaeRMRG-------GVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTA 417
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 531 EIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEE-PTEALRLELKNwvRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:PRK07867 418 PIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKfDPDAFAEFLAA--QPDLGPKQWPSYVRVCAELPRTATFK 495
|
....*...
gi 734849183 610 IMRRILRK 617
Cdd:PRK07867 496 VLKRQLSA 503
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
87-626 |
3.89e-24 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 106.63 E-value: 3.89e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 87 AERGDQAAIIWEGDDPAEsrtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhSVVFGGF 166
Cdd:PRK05857 24 ARQQPEAIALRRCDGTSA---LRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAI-AVMADGN 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 167 SPEALAGRIIDCKSKVVITADEGIRAGkkiplkanvddALTNPETSSIQKVIVCKRTNGAIKWNQHRDIWYedlmkvaga 246
Cdd:PRK05857 100 LPIAAIERFCQITDPAAALVAPGSKMA-----------SSAVPEALHSIPVIAVDIAAVTRESEHSLDAAS--------- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 247 VCAPKEMGAEEALFILYTSGSTGKPKGVqhttggylLYAALTHERVFD-YRPGEIYWctadVGWVTGHTyiVYGPLAngA 325
Cdd:PRK05857 160 LAGNADQGSEDPLAMIFTSGTTGEPKAV--------LLANRTFFAVPDiLQKEGLNW----VTWVVGET--TYSPLP--A 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 326 T---------TLLFEG---VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAceGADGSSLRLLGSVGEPInpE 393
Cdd:PRK05857 224 ThigglwwilTCLMHGglcVTGGENTTSLLEILTTNAVATTCLVPTLLSKLVSELKSA--NATVPSLRLVGYGGSRA--I 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 394 AWDWYYKNVGQSRCPIVdtWWQTETGGNMMSpLP----GAHALKPGSAARPFFGVVPALVD---------------NLGN 454
Cdd:PRK05857 300 AADVRFIEATGVRTAQV--YGLSETGCTALC-LPtddgSIVKIEAGAVGRPYPGVDVYLAAtdgigptapgagpsaSFGT 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 455 L-IEGPAEgnlvILDSWPGQARTlygdHDRFVDtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIE 533
Cdd:PRK05857 377 LwIKSPAN----MLGYWNNPERT----AEVLID-------GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVD 441
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 534 SAMVAHPKVAEAAVVGVPHDIKGQ--GIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:PRK05857 442 RIAEGVSGVREAACYEIPDEEFGAlvGLAVVASAELDESAARALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVM 521
|
570
....*....|....*
gi 734849183 612 RRILRKIATGEYDGL 626
Cdd:PRK05857 522 RASLAAAATADKARV 536
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
106-617 |
3.90e-24 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 106.76 E-value: 3.90e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTiTYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK06018 39 RT-TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVIT 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 aDEGIragkkIPLKANVDDALtnpetSSIQKVIVCkrTNGAIKWNQH--RDIWYEDLMKVAGAVCAPKEMGAEEALFILY 263
Cdd:PRK06018 118 -DLTF-----VPILEKIADKL-----PSVERYVVL--TDAAHMPQTTlkNAVAYEEWIAEADGDFAWKTFDENTAAGMCY 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHER-VFDYRPGEIY-----------WCTADVGWVTGHTYIVYGPLANGATtllfe 331
Cdd:PRK06018 185 TSGTTGDPKGVLYSHRSNVLHALMANNGdALGTSAADTMlpvvplfhansWGIAFSAPSMGTKLVMPGAKLDGAS----- 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 332 gvpnypditrVAKIVDKHKVNILYTAPTAIRAMMASGTAacegaDGSSLRLLGSV--GEPINPEAWDWYYKNVGqsrCPI 409
Cdd:PRK06018 260 ----------VYELLDTEKVTFTAGVPTVWLMLLQYMEK-----EGLKLPHLKMVvcGGSAMPRSMIKAFEDMG---VEV 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 410 VDTWWQTEtggnmMSPLPGAHALKPGSA--------------ARPFFGVVPALVDNLGNLI--EGPAEGNLVIldSWPGQ 473
Cdd:PRK06018 322 RHAWGMTE-----MSPLGTLAALKPPFSklpgdarldvlqkqGYPPFGVEMKITDDAGKELpwDGKTFGRLKV--RGPAV 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 474 ARTLYGDHDRFVDTyfktfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHD 553
Cdd:PRK06018 395 AAAYYRVDGEILDD-----DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHP 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 554 IKGQGIYVYVTLNGGEEPTealRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK06018 470 KWDERPLLIVQLKPGETAT---REEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
260-619 |
5.53e-24 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 103.18 E-value: 5.53e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 260 FILYTSGSTGKPKGVQHTTGGYLLYAALTHERV-FDyrpGEIYW-CTADVGWVTGhTYIVYGPLANGATTLLFEGVPnyp 337
Cdd:cd17630 4 TVILTSGSTGTPKAVVHTAANLLASAAGLHSRLgFG---GGDSWlLSLPLYHVGG-LAILVRSLLAGAELVLLERNQ--- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 338 ditrvAKIVDKHKVNILYTA--PTAIRAMMASGTAAcegADGSSLRLLGSVGEPINPEAWDwyykNVGQSRCPIVDTWWQ 415
Cdd:cd17630 77 -----ALAEDLAPPGVTHVSlvPTQLQRLLDSGQGP---AALKSLRAVLLGGAPIPPELLE----RAADRGIPLYTTYGM 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 416 TETGGNMMSPLPGAHALkpGSAARPFFGVVPALVDNlgnliegpaeGNLvildsWPGqARTLYGDHDRFVDTYFKTFRGM 495
Cdd:cd17630 145 TETASQVATKRPDGFGR--GGVGVLLPGRELRIVED----------GEI-----WVG-GASLAMGYLRGQLVPEFNEDGW 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 496 YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTeal 575
Cdd:cd17630 207 FTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPA--- 283
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 734849183 576 rlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd17630 284 --ELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
82-618 |
1.80e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 106.40 E-value: 1.80e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK12467 3101 IEAQVARTPEAPALVFGD------QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVP 3174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADegiRAGKKIPLKANVddaltnpetssiqKVIVCKRTngaikwnqhrdiwyeDLM 241
Cdd:PRK12467 3175 LDPEYPRERLAYMIEDSGVKLLLTQA---HLLEQLPAPAGD-------------TALTLDRL---------------DLN 3223
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KVAGAVCAPKEMGaEEALFILYTSGSTGKPKGV--------QHTTGGYLLYAALTHERV-------FDYRPGEIYWctad 306
Cdd:PRK12467 3224 GYSENNPSTRVMG-ENLAYVIYTSGSTGKPKGVgvrhgalaNHLCWIAEAYELDANDRVllfmsfsFDGAQERFLW---- 3298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 307 vgwvtghtyivygPLANGATTLLFEGVPNYPDITRVAkiVDKHKVNILYTAPTAIRAMMASGtaacEGADGSSLRLLGSV 386
Cdd:PRK12467 3299 -------------TLICGGCLVVRDNDLWDPEELWQA--IHAHRISIACFPPAYLQQFAEDA----GGADCASLDIYVFG 3359
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 387 GEPINPEAWDWY---------YKNVGQSRCPIVDTWWqtETGGNMMsplPGAHALKPGsaaRPFFGVVPALVDNLGNLIE 457
Cdd:PRK12467 3360 GEAVPPAAFEQVkrklkprglTNGYGPTEAVVTVTLW--KCGGDAV---CEAPYAPIG---RPVAGRSIYVLDGQLNPVP 3431
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 458 GPAEGNLVIldSWPGQARtlyGDH-------DRFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGT 529
Cdd:PRK12467 3432 VGVAGELYI--GGVGLAR---GYHqrpsltaERFVADPFSGSGGrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIEL 3506
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 530 AEIESAMVAHPKVAEAAVVGVPHDiKGQGIYVYVTLNggeEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:PRK12467 3507 GEIEARLLQHPSVREAVVLARDGA-GGKQLVAYVVPA---DPQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGK 3582
|
....*....
gi 734849183 610 IMRRILRKI 618
Cdd:PRK12467 3583 VDRKALPDP 3591
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
104-615 |
2.90e-23 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 103.71 E-value: 2.90e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGaihsvvfGGFSP-------EALAGRII 176
Cdd:cd17656 10 ENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAG-------GAFVPidpeypeERRIYIML 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 177 DCKSKVVITADEgiragkkIPLKANVDDALTNPETSSIQKVIvckrtngaikwNQHRDIWYEdlmkvagavcapkemgAE 256
Cdd:cd17656 83 DSGVRVVLTQRH-------LKSKLSFNKSTILLEDPSISQED-----------TSNIDYINN----------------SD 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 257 EALFILYTSGSTGKPKGVQ-------------------HTTGGYLLYAALThervFDYRPGEIY--WCTAdvgwvtGHTY 315
Cdd:cd17656 129 DLLYIIYTSGTTGKPKGVQlehknmvnllhferektniNFSDKVLQFATCS----FDVCYQEIFstLLSG------GTLY 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 316 IVygplaNGATTLlfegvpnypDITRVAKIVDKHKVNILYTaPTAIRAMMASGTAACEG-ADGssLRLLGSVGEP--INP 392
Cdd:cd17656 199 II-----REETKR---------DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRfPTC--VKHIITAGEQlvITN 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 393 EAWDWY-------YKNVGQSRCPIVDTWwqtetggnMMSPLPGAHALKPgsAARPFFGVVPALVDNLGNLIEGPAEGNLV 465
Cdd:cd17656 262 EFKEMLhehnvhlHNHYGPSETHVVTTY--------TINPEAEIPELPP--IGKPISNTWIYILDQEQQLQPQGIVGELY 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 466 IldSWPGQARTLYGD----HDRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPK 541
Cdd:cd17656 332 I--SGASVARGYLNRqeltAEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPG 409
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 542 VAEAAVVGVPHDIKGQGIYVYVTlnggeePTEALRL-ELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17656 410 VSEAVVLDKADDKGEKYLCAYFV------MEQELNIsQLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
84-619 |
4.40e-23 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 103.01 E-value: 4.40e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLAERGDQAAI-IWEGDdpaesrtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhSVV 162
Cdd:cd05918 7 ERARSQPDAPAVcAWDGS-------LTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGGA-FVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 FGGFSPEA-LAGRIIDCKSKVVITADegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlm 241
Cdd:cd05918 79 LDPSHPLQrLQEILQDTGAKVVLTSS------------------------------------------------------ 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 kvagavcapkemgAEEALFILYTSGSTGKPKGV--QHTTggyLLYAALTHERVFDYRPGEIYWCTA----DVgwvtgHTY 315
Cdd:cd05918 105 -------------PSDAAYVIFTSGSTGKPKGVviEHRA---LSTSALAHGRALGLTSESRVLQFAsytfDV-----SIL 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 316 IVYGPLANGATTLlfegVPNYPD-ITRVAKIVDKHKVNILYTAPTAIRAMMASGTaacegadgSSLRLLGSVGEPINPEa 394
Cdd:cd05918 164 EIFTTLAAGGCLC----IPSEEDrLNDLAGFINRLRVTWAFLTPSVARLLDPEDV--------PSLRTLVLGGEALTQS- 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 395 wdwyyknvgqsrcpIVDTWWQ----------TET--GGNMMSPLPGAHALKPGSAarpfFGVVPALVD--NLGNLIEGPA 460
Cdd:cd05918 231 --------------DVDTWADrvrlinaygpAECtiAATVSPVVPSTDPRNIGRP----LGATCWVVDpdNHDRLVPIGA 292
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 461 EGNLVIldSWPGQARTLYGDHDR----FVDT-------YFKTFRGMYFTGDGARRDEDG--YYwiTGRVDDVLNVSGHRM 527
Cdd:cd05918 293 VGELLI--EGPILARGYLNDPEKtaaaFIEDpawlkqeGSGRGRRLYRTGDLVRYNPDGslEY--VGRKDTQVKIRGQRV 368
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 528 GTAEIESAMVAHPKVAEAAVVGV--PHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIAS------------- 592
Cdd:cd05918 369 ELGEIEHHLRQSLPGAKEVVVEVvkPKDGSSSPQLVAFVVLDGSSSGSGDGDSLFLEPSDEFRALVAelrsklrqrlpsy 448
|
570 580 590
....*....|....*....|....*....|.
gi 734849183 593 --PDViqWAP--GLPKTRSGKIMRRILRKIA 619
Cdd:cd05918 449 mvPSV--FLPlsHLPLTASGKIDRRALRELA 477
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
82-615 |
9.49e-23 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 104.48 E-value: 9.49e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDdpaesrTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK05691 1137 LNEQARQTPERIALVWDGG------SLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVP 1210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITadegiragkkiplKANVDDALTNPETSSiqkvivckrtngAIKWNQ-HRDIWyedl 240
Cdd:PRK05691 1211 LDPDYPAERLAYMLADSGVELLLT-------------QSHLLERLPQAEGVS------------AIALDSlHLDSW---- 1261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 mkvagAVCAPK-EMGAEEALFILYTSGSTGKPKGVQHTTGG-----------YllyaALTHERV--------FDYRPGEI 300
Cdd:PRK05691 1262 -----PSQAPGlHLHGDNLAYVIYTSGSTGQPKGVGNTHAAlaerlqwmqatY----ALDDSDVlmqkapisFDVSVWEC 1332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 301 YWctadvgwvtghtyivygPLANGATtLLFEGVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMAS-GTAACegadgSS 379
Cdd:PRK05691 1333 FW-----------------PLITGCR-LVLAGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEpLAAAC-----TS 1389
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 380 LRLLGSVGEPINPEAWD---------WYYKNVGQSRCPIVDTWWQTETGGNMMSPLpgahalkpgsaARPFFGVVPALVD 450
Cdd:PRK05691 1390 LRRLFSGGEALPAELRNrvlqrlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI-----------GRPLGNVLCRVLD 1458
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 451 NLGNLIEGPAEGNLVIldSWPGQARTLYG----DHDRFV-DTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGH 525
Cdd:PRK05691 1459 AELNLLPPGVAGELCI--GGAGLARGYLGrpalTAERFVpDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGF 1536
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 526 RMGTAEIESAMVAHPKVAEAAVVgVPHDIKGQGIYVYVTLNGG-EEPTE----ALRLELKNW-VRKEIGPIASpdviqwa 599
Cdd:PRK05691 1537 RVEPEEIQARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAGqEAEAErlkaALAAELPEYmVPAQLIRLDQ------- 1608
|
570
....*....|....*.
gi 734849183 600 pgLPKTRSGKIMRRIL 615
Cdd:PRK05691 1609 --MPLGPSGKLDRRAL 1622
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
81-621 |
2.00e-22 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 101.60 E-value: 2.00e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 81 CLDRhLAERGDQAAIIwegdDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHS 160
Cdd:PLN02246 29 CFER-LSEFSDRPCLI----DGATGRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTT 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 161 VVFGGFSPEALAGRIIDCKSKVVITADegiRAGKKIPLKANVDDAltnpetssiqkVIVCKrtngaikwNQHRD--IWYE 238
Cdd:PLN02246 104 TANPFYTPAEIAKQAKASGAKLIITQS---CYVDKLKGLAEDDGV-----------TVVTI--------DDPPEgcLHFS 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQhttggyllyaaLTHE----RVFDYRPGE---IYWCTADVGWVT 311
Cdd:PLN02246 162 ELTQADENELPEVEISPDDVVALPYSSGTTGLPKGVM-----------LTHKglvtSVAQQVDGEnpnLYFHSDDVILCV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 312 ---GHTY----IVYGPLANGATTLLfegVPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLG 384
Cdd:PLN02246 231 lpmFHIYslnsVLLCGLRVGAAILI---MPKF-EIGALLELIQRHKVTIAPFVPPIVLAIAKS--PVVEKYDLSSIRMVL 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 385 SVGEPINPEAWDWYyknvgQSRCP---IVDTWWQTETGGNM-MS------PLPgahaLKPGSAarpffGVVpalVDNlgn 454
Cdd:PLN02246 305 SGAAPLGKELEDAF-----RAKLPnavLGQGYGMTEAGPVLaMClafakePFP----VKSGSC-----GTV---VRN--- 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 455 liegpAEGNLVILDSWPGQARTLYGD----HDRFVDTYFK----TFR-----GMYFTGDGARRDEDGYYWITGRVDDVLN 521
Cdd:PLN02246 365 -----AELKIVDPETGASLPRNQPGEicirGPQIMKGYLNdpeaTANtidkdGWLHTGDIGYIDDDDELFIVDRLKELIK 439
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 522 VSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPG 601
Cdd:PLN02246 440 YKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITED---EIKQFVAKQVVFYKRIHKVFFVDS 516
|
570 580
....*....|....*....|.
gi 734849183 602 LPKTRSGKIMRRILR-KIATG 621
Cdd:PLN02246 517 IPKAPSGKILRKDLRaKLAAG 537
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
104-617 |
3.28e-22 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 99.69 E-value: 3.28e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHsvvfggfspealagriidckskvv 183
Cdd:cd17653 19 LGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAY------------------------ 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 itadegiragkkIPLkanvddaltNPETSSIQKVIVCKRTNGAIkwnqhrdiwyedlmkvagAVCAPKemgAEEALFILY 263
Cdd:cd17653 75 ------------VPL---------DAKLPSARIQAILRTSGATL------------------LLTTDS---PDDLAYIIF 112
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPGE--------IYWCTADVgwvtghtyiVYGPLANGATTLLFEgvPN 335
Cdd:cd17653 113 TSGSTGIPKGVMVPHRGVLNYVSQPPAR-LDVGPGSrvaqvlsiAFDACIGE---------IFSTLCNGGTLVLAD--PS 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 336 YPdITRVAKivdkhKVNILYTAPTAIrammasgtAACEGADGSSLRLLGSVGEPINPeawdwyyknvgqsrcPIVDTWWQ 415
Cdd:cd17653 181 DP-FAHVAR-----TVDALMSTPSIL--------STLSPQDFPNLKTIFLGGEAVPP---------------SLLDRWSP 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 416 ----------TET--GGNMMSPLPGahalKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDH-- 481
Cdd:cd17653 232 grrlynaygpTECtiSSTMTELLPG----QPVTIGKPIPNSTCYILDADLQPVPEGVVGEICI--SGVQVARGYLGNPal 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 --DRFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVA-HPKVAEAAVVGVphdikGQG 558
Cdd:cd17653 306 taSKFVPDPFWPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQsQPEVTQAAAIVV-----NGR 380
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 559 IYVYVTlnggeePTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd17653 381 LVAFVT------PETVDVDGLRSELAKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
108-617 |
4.24e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 102.34 E-value: 4.24e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAD 187
Cdd:PRK12316 3083 LSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLLSQS 3162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EgiragkkiplkanvddaLTNPETSSIQKVIVCKRTNGAIKWNqhrdiwyedlmkvagavcAPKEMGAEEALFILYTSGS 267
Cdd:PRK12316 3163 H-----------------LRLPLAQGVQVLDLDRGDENYAEAN------------------PAIRTMPENLAYVIYTSGS 3207
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 268 TGKPKGV--------QHTTGGYLLYAALTHERV-------FDYRPGEIYWctadvgwvtghtyivygPLANGATTLLfEG 332
Cdd:PRK12316 3208 TGKPKGVgirhsalsNHLCWMQQAYGLGVGDRVlqfttfsFDVFVEELFW-----------------PLMSGARVVL-AG 3269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAcegaDGSSLRLLGSVGEPINPEAWDWYykNVGQsrcPIVDT 412
Cdd:PRK12316 3270 PEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAH----RCTSLKRIVCGGEALPADLQQQV--FAGL---PLYNL 3340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 413 WWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARTLYGDHD----RFVDTY 488
Cdd:PRK12316 3341 YGPTEATITVTHWQCVEEGKDAVPIGRPIANRACYILDGSLEPVPVGALGELYL--GGEGLARGYHNRPGltaeRFVPDP 3418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 489 FKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVgvphDIKGQGIYVYVTLngg 568
Cdd:PRK12316 3419 FVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVL----AVDGRQLVAYVVP--- 3491
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 734849183 569 EEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK12316 3492 EDEAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPR 3540
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
104-617 |
8.61e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 101.19 E-value: 8.61e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:PRK12316 4573 DEEKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALL 4652
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITADegiRAGKKIPLKANVDDALTNPEtssiqkvivckrtngaikwnqhrDIWyEDLMKVAGAVcapkEMGAEEALFILY 263
Cdd:PRK12316 4653 LTQS---HLLQRLPIPDGLASLALDRD-----------------------EDW-EGFPAHDPAV----RLHPDNLAYVIY 4701
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHERvfdyrpgeiYWCTAD--VGWVTGHTYIV-----YGPLANGATTLLFEgvPNY 336
Cdd:PRK12316 4702 TSGSTGRPKGVAVSHGSLVNHLHATGER---------YELTPDdrVLQFMSFSFDGsheglYHPLINGASVVIRD--DSL 4770
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 PDITRVAKIVDKHKVNILYTAPTAIRaMMASGTAacEGADGSSLRLLGSVGEPINPEAWDWYYKNV---------GQSRC 407
Cdd:PRK12316 4771 WDPERLYAEIHEHRVTVLVFPPVYLQ-QLAEHAE--RDGEPPSLRVYCFGGEAVAQASYDLAWRALkpvylfngyGPTET 4847
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 408 PIVDTWWQTETGgnmmsPLPGAHALKPGsaaRPFFGVVPALVDNLGNLIEGPAEGNLVIldSWPGQARtlyGDH------ 481
Cdd:PRK12316 4848 TVTVLLWKARDG-----DACGAAYMPIG---TPLGNRSGYVLDGQLNPLPVGVAGELYL--GGEGVAR---GYLerpalt 4914
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 -DRFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIkGQGI 559
Cdd:PRK12316 4915 aERFVPDPFGAPGGrLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GKQL 4993
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 734849183 560 YVYVT-----LNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK12316 4994 VGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQ 5056
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-609 |
1.75e-21 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 96.68 E-value: 1.75e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGV---QHTTGGYLLYAALTHERVFDyrpgEIYWCTADVGWVTGHTYIVYGPLANGATTLLFEG 332
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVmwrQEDIFRMLMGGADFGTGEFT----PSEDAHKAAAAAAGTVMFPAPPLMHGTGSWTAFG 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 VPN------YPDI----TRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNV 402
Cdd:cd05924 79 GLLggqtvvLPDDrfdpEEVWRTIEKHKVTSMTIVGDAMARPLIDALRDAGPYDLSSLFAISSGGALLSPEVKQGLLELV 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 GQSRcpIVDTWWQTETGGNMMSplpgaHALKPGSAARPFFGVVP--ALVDNLGNLIEGPAEGnlvildswPGQ-ART--- 476
Cdd:cd05924 159 PNIT--LVDAFGSSETGFTGSG-----HSAGSGPETGPFTRANPdtVVLDDDGRVVPPGSGG--------VGWiARRghi 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 477 ---LYGDHDRFVDTyFKTFRGM--YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVP 551
Cdd:cd05924 224 plgYYGDEAKTAET-FPEVDGVryAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRP 302
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 552 HDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:cd05924 303 DERWGQEVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
257-612 |
1.83e-21 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 95.80 E-value: 1.83e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 257 EALFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWCTADVGWVTGhtyivygplANGATTLLFEGVPNY 336
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAG---------LNLALATFHAGGANV 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 P----DITRVAKIVDKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLGSVGEPINPEAWdwyyknvgQSRCPivDT 412
Cdd:cd17637 71 VmekfDPAEALELIEEEKVTLMGSFPPILSNLLD--AAEKSGVDLSSLRHVLGLDAPETIQRF--------EETTG--AT 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 413 WW----QTETGGNM-MSPlpgaHALKPGSAARPFFGVVPALVDNLGN----------LIEGPaegnLVILDSWPGQARTL 477
Cdd:cd17637 139 FWslygQTETSGLVtLSP----YRERPGSAGRPGPLVRVRIVDDNDRpvpagetgeiVVRGP----LVFQGYWNLPELTA 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 478 YgdhdrfvdtyfkTFR-GMYFTGDGARRDEDGYYWITGRV--DDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPhDI 554
Cdd:cd17637 211 Y------------TFRnGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVP-DP 277
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 555 K-GQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17637 278 KwGEGIKAVCVLKPGATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
85-617 |
2.01e-21 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 100.03 E-value: 2.01e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 85 HLAERGDQAAIIWEGDdpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFG 164
Cdd:PRK12316 2011 EQAARAPEAIAVVFGD-----QHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDP 2085
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 165 GFSPEALAGRIIDCKSKVVITaDEGIRAgkKIPLKANVDDALTNpetssiqkvivckrtngaikwnqhRDIWYEDLMKVA 244
Cdd:PRK12316 2086 NYPAERLAYMLEDSGAALLLT-QRHLLE--RLPLPAGVARLPLD------------------------RDAEWADYPDTA 2138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 245 GAVcapkEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPGEIYWCTADVGWVTGHTYIvYGPLANG 324
Cdd:PRK12316 2139 PAV----QLAGENLAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER-YELSPADCELQFMSFSFDGAHEQW-FHPLLNG 2212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 325 ATTLLFEGVPNYPDitRVAKIVDKHKVNILYTAPTAIRAMmasgtAACEGADGS--SLRLLGSVGEPINPEAWDWYYKNV 402
Cdd:PRK12316 2213 ARVLIRDDELWDPE--QLYDEMERHGVTILDFPPVYLQQL-----AEHAERDGRppAVRVYCFGGEAVPAASLRLAWEAL 2285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 GQSRcpIVDTWWQTETggnMMSPLpgAHALKPGSAARPFFGVVPALVDNLGNLIEGpAEGNLVIldswPGQARTLY---- 478
Cdd:PRK12316 2286 RPVY--LFNGYGPTEA---VVTPL--LWKCRPQDPCGAAYVPIGRALGNRRAYILD-ADLNLLA----PGMAGELYlgge 2353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 ----GDHD-------RFV-DTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAA 546
Cdd:PRK12316 2354 glarGYLNrpgltaeRFVpDPFSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAVREAV 2433
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 734849183 547 VVGVpHDIKGQGIYVYVTlngGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK12316 2434 VVAQ-DGASGKQLVAYVV---PDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPK 2500
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
249-616 |
2.81e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 97.37 E-value: 2.81e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 249 APKEMGAEEALFILYTSGSTGKPKGVQhttggyLLYAALTHErvFDYRPGEIYWCTADV-----------GWVTGhtyiV 317
Cdd:PRK07787 121 RYPEPDPDAPALIVYTSGTTGPPKGVV------LSRRAIAAD--LDALAEAWQWTADDVlvhglplfhvhGLVLG----V 188
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGPLANGAT--------------------TLLFeGVPnypdiTRVAKIVDkhkvnilytAPTAIRA-----MMASGTAAC 372
Cdd:PRK07787 189 LGPLRIGNRfvhtgrptpeayaqalseggTLYF-GVP-----TVWSRIAA---------DPEAARAlrgarLLVSGSAAL 253
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 373 EGADGSSLRLLgsvgepinpeawdwyyknVGQsrcPIVDTWWQTETGGNMMSPLPGAHalKPGSAARPFFGVVPALVDNL 452
Cdd:PRK07787 254 PVPVFDRLAAL------------------TGH---RPVERYGMTETLITLSTRADGER--RPGWVGLPLAGVETRLVDED 310
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 453 GNLIEGPAE--GNLVIldswpgQARTLYGDHDRFVDTYFKTFR--GMYFTGDGARRDEDGYYWITGRVD-DVLNVSGHRM 527
Cdd:PRK07787 311 GGPVPHDGEtvGELQV------RGPTLFDGYLNRPDATAAAFTadGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRI 384
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 528 GTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTlnGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRS 607
Cdd:PRK07787 385 GAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVV--GADDVAAD---ELIDFVAQQLSVHKRPREVRFVDALPRNAM 459
|
....*....
gi 734849183 608 GKIMRRILR 616
Cdd:PRK07787 460 GKVLKKQLL 468
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
48-620 |
4.18e-21 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 97.61 E-value: 4.18e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 48 WIKPFTTVKQTSFDDHHVDikwfADGTLNVSYNCLDRHlAERGDQAAIiwegdDPAESRTITYRELHEEVCKFANALRGQ 127
Cdd:PLN02574 17 WYSPETGIYSSKHPPVPLP----SDPNLDAVSFIFSHH-NHNGDTALI-----DSSTGFSISYSELQPLVKSMAAGLYHV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 128 -DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGIraGKKIPLKANVddaL 206
Cdd:PLN02574 87 mGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTSPENV--EKLSPLGVPV---I 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 207 TNPETSSIQkvivckrtNGAIKWNQHRDIWYEDlmkvaGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYL---- 282
Cdd:PLN02574 162 GVPENYDFD--------SKRIEFPKFYELIKED-----FDFVPKPVIKQDDVAAIMYSSGTTGASKGVVLTHRNLIamve 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 283 --------LYAALTHERVfdyrpgeiYWCTADVGWVTGHTYIVYGPLANGATTLLFEGVpnypDITRVAKIVDKHKVNIL 354
Cdd:PLN02574 229 lfvrfeasQYEYPGSDNV--------YLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF----DASDMVKVIDRFKVTHF 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 355 YTAPTAIRAMMASGTAACEGADGSsLRLLGSVGEPINPEAWDWYYKNVgqSRCPIVDTWWQTET------GGNM--MSPL 426
Cdd:PLN02574 297 PVVPPILMALTKKAKGVCGEVLKS-LKQVSCGAAPLSGKFIQDFVQTL--PHVDFIQGYGMTEStavgtrGFNTekLSKY 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 427 PGAHALKPGSAARPFFGVVPALV--DNLGNL-IEGPA--EGNLVIldswpGQARTLYGDHDrfvdtyfktfrGMYFTGDG 501
Cdd:PLN02574 374 SSVGLLAPNMQAKVVDWSTGCLLppGNCGELwIQGPGvmKGYLNN-----PKATQSTIDKD-----------GWLRTGDI 437
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 502 ARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKN 581
Cdd:PLN02574 438 AYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE---AVIN 514
|
570 580 590
....*....|....*....|....*....|....*....
gi 734849183 582 WVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIAT 620
Cdd:PLN02574 515 YVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRSLT 553
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
256-612 |
4.60e-21 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 95.02 E-value: 4.60e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGVqhTTGGYLLYAALTHERVfdyrpGEIYWCTADVGWVTGHTYIVYGpLANGATTLLFEGV-- 333
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAV--LLANKTFFAVPDILQK-----EGLNWVVGDVTYLPLPATHIGG-LWWILTCLIHGGLcv 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 334 --PNYPDITRVAKIVDKHKVNILYTAPTA---IRAMMASGTAACEgadgsSLRLLGSVGE-PINPE-AWDWYYKNVGqsr 406
Cdd:cd17635 73 tgGENTTYKSLFKILTTNAVTTTCLVPTLlskLVSELKSANATVP-----SLRLIGYGGSrAIAADvRFIEATGLTN--- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 407 cpIVDTWWQTETGGNMMSPLpGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSW--PGQARTLYGDHDRF 484
Cdd:cd17635 145 --TAQVYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPAnmLGYWNNPERTAEVL 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 485 VDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVT 564
Cdd:cd17635 222 IDGWVNT-------GDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVV 294
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 734849183 565 LNGGEEPTEALRLELKnwVRKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17635 295 ASAELDENAIRALKHT--IRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
86-616 |
4.61e-21 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 97.79 E-value: 4.61e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 86 LAERGDQAAiiWEgDDPA--ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLAC-ARigaihsvv 162
Cdd:PRK06060 10 LAEQASEAG--WY-DRPAfyAADVVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLAClAR-------- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 163 fggfspealagriidckskvvitadeGIRAGKKIPLKANVDDALtnPETSSIQKVIVckrTNGAI--KWNQHRDIWYEDL 240
Cdd:PRK06060 79 --------------------------GVMAFLANPELHRDDHAL--AARNTEPALVV---TSDALrdRFQPSRVAEAAEL 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MKVAGAVcAPKE---MGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIV 317
Cdd:PRK06060 128 MSEAARV-APGGyepMGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSV 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 318 YGPLANGATTLLfEGVPNYPDITrvAKIVDKHKVNILYTAPTairaMMASGTAACEGADGSSLRLLGSVGEPINPEAWDW 397
Cdd:PRK06060 207 WFPLATGGSAVI-NSAPVTPEAA--AILSARFGPSVLYGVPN----FFARVIDSCSPDSFRSLRCVVSAGEALELGLAER 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGQsrCPIVDTWWQTETGGNMMSPlpGAHALKPGSAARpffgVVPA----LVDNLGNLIEGPAEGNLvildsW--- 470
Cdd:PRK06060 280 LMEFFGG--IPILDGIGSTEVGQTFVSN--RVDEWRLGTLGR----VLPPyeirVVAPDGTTAGPGVEGDL-----Wvrg 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 471 PGQARTLYGDHDRFVdtyfkTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV 550
Cdd:PRK06060 347 PAIAKGYWNRPDSPV-----ANEGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAV 421
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 551 PHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK06060 422 RESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
100-619 |
1.03e-20 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 96.10 E-value: 1.03e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDPAES---RTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRI 175
Cdd:PRK08751 40 DRPAYHsfgKTITYREADQLVEQFAAYLLGElQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 176 IDCKSKVVITAD------EGIRAGKKIP--LKANVDDALTNPETS-------SIQKVIVCKRTNGAIKWNQHRDIWYEDL 240
Cdd:PRK08751 120 IDSGASVLVVIDnfgttvQQVIADTPVKqvITTGLGDMLGFPKAAlvnfvvkYVKKLVPEYRINGAIRFREALALGRKHS 199
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MkvagavcAPKEMGAEEALFILYTSGSTGKPKGvqhttggyllyAALTHERVFDYRPGEIYWCTADVGWVTGHT------ 314
Cdd:PRK08751 200 M-------PTLQIEPDDIAFLQYTGGTTGVAKG-----------AMLTHRNLVANMQQAHQWLAGTGKLEEGCEvvital 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 315 --YIVYGPLANGATTLLFEG----VPNYPDITRVAKIVDKHKvnilYTAPTAIRAMMAS--GTAACEGADGSSLRLLGSV 386
Cdd:PRK08751 262 plYHIFALTANGLVFMKIGGcnhlISNPRDMPGFVKELKKTR----FTAFTGVNTLFNGllNTPGFDQIDFSSLKMTLGG 337
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 387 GEPINPEAWDWYYKNVGqsrCPIVDTWWQTETG-GNMMSPLpgahALKP--GSAARPFFGVVPALVDNLGNLIEGPAEGN 463
Cdd:PRK08751 338 GMAVQRSVAERWKQVTG---LTLVEAYGLTETSpAACINPL----TLKEynGSIGLPIPSTDACIKDDAGTVLAIGEIGE 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 464 LVIldswpGQARTLYGDHDRFVDT-YFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKV 542
Cdd:PRK08751 411 LCI-----KGPQVMKGYWKRPEETaKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGV 485
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 543 AEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEalrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK08751 486 LEVAAVGVPDEKSGEIVKVVIVKKDPALTAE----DVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDAA 558
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
109-617 |
1.34e-20 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 95.58 E-value: 1.34e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:cd05915 26 TYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLLFDPN 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 GIR-AGKKIPLKANVDDaltNPETSSiqkvivckrtngaiKWNQhrdiwYEDLMKVAGAVCAP-KEMGAEEALFILYTSG 266
Cdd:cd05915 106 LLPlVEAIRGELKTVQH---FVVMDE--------------KAPE-----GYLAYEEALGEEADpVRVPERAACGMAYTTG 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 267 STGKPKGVQHT-TGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIvYGPLANGATTLLFEGVPNypDITRVAKI 345
Cdd:cd05915 164 TTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLP-YAATLVGAKQVLPGPRLD--PASLVELF 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 346 VdKHKVNILYTAPTAIRaMMASGTAACEGADGSSLRLLGSVGEPinPEAWdWYYKNVGQSRCPIVDTWWQTETGGNMMSP 425
Cdd:cd05915 241 D-GEGVTFTAGVPTVWL-ALADYLESTGHRLKTLRRLVVGGSAA--PRSL-IARFERMGVEVRQGYGLTETSPVVVQNFV 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LPGAHALKPGSA----ARPFFGVVPALVDNLG-NLIEGPAEGNLVILDSWPGQArTLYGDHDRFVDTYFKTFRG-MYFTG 499
Cdd:cd05915 316 KSHLESLSEEEKltlkAKTGLPIPLVRLRVADeEGRPVPKDGKALGEVQLKGPW-ITGGYYGNEEATRSALTPDgFFRTG 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 500 DGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEalrlEL 579
Cdd:cd05915 395 DIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPRGEKPTPE----EL 470
|
490 500 510
....*....|....*....|....*....|....*....
gi 734849183 580 KNWVRKEIGPIAS-PDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05915 471 NEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
100-616 |
1.99e-20 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 95.22 E-value: 1.99e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDPAES---RTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsVVFGG--FSPEALAG 173
Cdd:PRK05677 39 DKPAFSnlgKTLTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VVNTNplYTAREMEH 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 174 RIIDCKSK-VVITADEGIRAGKKIP-------LKANVDD-------ALTNPETSSIQKVIVCKRTNGAIKWNqhrdiwyE 238
Cdd:PRK05677 117 QFNDSGAKaLVCLANMAHLAEKVLPktgvkhvIVTEVADmlpplkrLLINAVVKHVKKMVPAYHLPQAVKFN-------D 189
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGvqhttggyllyAALTHERVFdyrpGEIYWCTADVGWVTGH----- 313
Cdd:PRK05677 190 ALAKGAGQPVTEANPQADDVAVLQYTGGTTGVAKG-----------AMLTHRNLV----ANMLQCRALMGSNLNEgceil 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 314 -----TYIVYGPLANGATTLLFEG----VPNYPDITRVAKIVDKHK------VNILYTA---PTAIRAMmasgtaacega 375
Cdd:PRK05677 255 iaplpLYHIYAFTFHCMAMMLIGNhnilISNPRDLPAMVKELGKWKfsgfvgLNTLFVAlcnNEAFRKL----------- 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 376 DGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETggnmmSPLPG---AHALKPGSAARPFFGVVPALVDNL 452
Cdd:PRK05677 324 DFSALKLTLSGGMALQLATAERWKEVTG---CAICEGYGMTET-----SPVVSvnpSQAIQVGTIGIPVPSTLCKVIDDD 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 453 GNLIEGPAEGNL------VILDSWPGQARTlygdhDRFVDTyfktfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHR 526
Cdd:PRK05677 396 GNELPLGEVGELcvkgpqVMKGYWQRPEAT-----DEILDS-----DGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFN 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 527 MGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTR 606
Cdd:PRK05677 466 VYPNELEDVLAALPGVLQCAAIGVPDEKSGEAIKVFVVVKPGETLTKE---QVMEHMRANLTGYKVPKAVEFRDELPTTN 542
|
570
....*....|
gi 734849183 607 SGKIMRRILR 616
Cdd:PRK05677 543 VGKILRRELR 552
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
109-618 |
3.25e-20 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 94.44 E-value: 3.25e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:PRK06155 48 TYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLVVEAA 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 GIRAgkkipLKANVDDALTNPETSSIqkvivckrtNGAIKWNQHRDIWYEDLMKVAGAVCA----PKEMGAeealfILYT 264
Cdd:PRK06155 128 LLAA-----LEAADPGDLPLPAVWLL---------DAPASVSVPAGWSTAPLPPLDAPAPAaavqPGDTAA-----ILYT 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 265 SGSTGKPKGVQHTTGGYLLYAALTHErVFDYRPGEIYWCTADVgWVTGHTYIVYGPLANGATTLL---FEGVPNYPDITR 341
Cdd:PRK06155 189 SGTTGPSKGVCCPHAQFYWWGRNSAE-DLEIGADDVLYTTLPL-FHTNALNAFFQALLAGATYVLeprFSASGFWPAVRR 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 342 vakivdkHKVNILYTaptaIRAM--MASGTAACEGADGSSLRLLGSVGEPinPEAWDWYYKNVGqsrCPIVDTWWQTETG 419
Cdd:PRK06155 267 -------HGATVTYL----LGAMvsILLSQPARESDRAHRVRVALGPGVP--AALHAAFRERFG---VDLLDGYGSTETN 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 420 GNMMSPLPGAhalKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSWPGQARTLY-GDHDRFVdtyfKTFRGMYF- 497
Cdd:PRK06155 331 FVIAVTHGSQ---RPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEPFAFATGYfGMPEKTV----EAWRNLWFh 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGE--EPTEAL 575
Cdd:PRK06155 404 TGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAVVLRDGTalEPVALV 483
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 734849183 576 RlelknWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK06155 484 R-----HCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
100-615 |
3.68e-20 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 95.50 E-value: 3.68e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDPA---ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRII 176
Cdd:PRK10252 473 DAPAladARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLE 552
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 177 DCKSKVVITADEgiragkkipLKANVDDAltnPETSSIQkvivckrtngaikwnqhrdiwYEDLMKVAGAvcAPKEMGA- 255
Cdd:PRK10252 553 DARPSLLITTAD---------QLPRFADV---PDLTSLC---------------------YNAPLAPQGA--APLQLSQp 597
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGV---QHTTGGYLL-----YAALTHERVFDYRPgeiywCTADVG-WVtghtyiVYGPLANGAT 326
Cdd:PRK10252 598 HHTAYIIFTSGSTGRPKGVmvgQTAIVNRLLwmqnhYPLTADDVVLQKTP-----CSFDVSvWE------FFWPFIAGAK 666
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 TLLFEgvpnyPDITR----VAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNV 402
Cdd:PRK10252 667 LVMAE-----PEAHRdplaMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPADLCREWQQLT 741
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 GqsrCPI----------VD-TWWqtETGGNMMSPLPGAhalkPGSAARPffgvvpalVDNLGnliegpaegnLVILDSW- 470
Cdd:PRK10252 742 G---APLhnlygpteaaVDvSWY--PAFGEELAAVRGS----SVPIGYP--------VWNTG----------LRILDARm 794
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 471 ----PGQARTLY-----------GDHD----RFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAE 531
Cdd:PRK10252 795 rpvpPGVAGDLYltgiqlaqgylGRPDltasRFIADPFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGE 874
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 532 IESAMVAHPKVAEAAVV------GVPHDIKGQGIYVYVTLNGGEEP-TEALRLELknwvRKEIGPIASPDVIQWAPGLPK 604
Cdd:PRK10252 875 IDRAMQALPDVEQAVTHacvinqAAATGGDARQLVGYLVSQSGLPLdTSALQAQL----RERLPPHMVPVVLLQLDQLPL 950
|
570
....*....|.
gi 734849183 605 TRSGKIMRRIL 615
Cdd:PRK10252 951 SANGKLDRKAL 961
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
84-617 |
3.87e-20 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 94.23 E-value: 3.87e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHLAERGDQAAIIWEGDDPAESRTITYRELHEEVCKFANALRgQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVF 163
Cdd:cd05931 1 RRAAARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQ-AVGKPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 164 GGFSPEA---LAGRIIDCKSKVVITADEgiragkkipLKANVDDALTNPETSSIQKVIVCkrtngaikwnqhrdiwyeDL 240
Cdd:cd05931 80 PPTPGRHaerLAAILADAGPRVVLTTAA---------ALAAVRAFAASRPAAGTPRLLVV------------------DL 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MKVAGA-VCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIY--W--CTADVGWVTGhty 315
Cdd:cd05931 133 LPDTSAaDWPPPSPDPDDIAYLQYTSGSTGTPKGVVVTHRN-LLANVRQIRRAYGLDPGDVVvsWlpLYHDMGLIGG--- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 316 iVYGPLANGATTLLFEGV-----PnypdiTRVAKIVDKHKVNILYtAPT-----AIRAMMASGTaacEGADGSSLRLLGS 385
Cdd:cd05931 209 -LLTPLYSGGPSVLMSPAaflrrP-----LRWLRLISRYRATISA-APNfaydlCVRRVRDEDL---EGLDLSSWRVALN 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 386 VGEPINPEAwdwyyknvgqsrcpiVDTWWQTetggnmMSP--LPgAHALKPG----------SAARPFFGVVPALVD--N 451
Cdd:cd05931 279 GAEPVRPAT---------------LRRFAEA------FAPfgFR-PEAFRPSyglaeatlfvSGGPPGTGPVVLRVDrdA 336
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 452 LGNLIEGPAEGnlvildswPGQARTL--YG----DHD-RFVDT------------------------YF-------KTFR 493
Cdd:cd05931 337 LAGRAVAVAAD--------DPAARELvsCGrplpDQEvRIVDPetgrelpdgevgeiwvrgpsvasgYWgrpeataETFG 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 494 -------GMYF-TGD-GARRdeDGYYWITGRVDDVLNVSGHRMGTAEIE-SAMVAHPKVAE--AAVVGVPHDIKGQGIYV 561
Cdd:cd05931 409 alaatdeGGWLrTGDlGFLH--DGELYITGRLKDLIIVRGRNHYPQDIEaTAEEAHPALRPgcVAAFSVPDDGEERLVVV 486
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 562 yVTLNGGEEPTE--ALRLELKNWVRKEIGpIASPDVIQWAPG-LPKTRSGKIMRRILRK 617
Cdd:cd05931 487 -AEVERGADPADlaAIAAAIRAAVAREHG-VAPADVVLVRPGsIPRTSSGKIQRRACRA 543
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
106-616 |
2.42e-19 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 92.01 E-value: 2.42e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVI- 184
Cdd:PRK07059 47 KAITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVv 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 185 ------TADEGIRagkKIPLK----ANVDD------ALTNPETSSIQKVIVCKRTNGAIKWNQHrdiwyedLMKVAGAVC 248
Cdd:PRK07059 127 lenfatTVQQVLA---KTAVKhvvvASMGDllgfkgHIVNFVVRRVKKMVPAWSLPGHVRFNDA-------LAEGARQTF 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 249 APKEMGAEEALFILYTSGSTGKPKGVQHTTGGYL---LYAALTHERVFDYRPGE---IYWCTADVGWVTGHTYIVYGPLA 322
Cdd:PRK07059 197 KPVKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVanvLQMEAWLQPAFEKKPRPdqlNFVCALPLYHIFALTVCGLLGMR 276
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 323 NGATTLLfegVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRL-LG---SVGEPInpeAWDWY 398
Cdd:PRK07059 277 TGGRNIL---IPNPRDIPGFIKELKKYQVHIFPAVNTLYNALLNN--PDFDKLDFSKLIVaNGggmAVQRPV---AERWL 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YKnvgqSRCPIVDTWWQTETggnmmSPLPGAHALK----PGSAARPFFGVVPALVDNLGN----------LIEGPAegnl 464
Cdd:PRK07059 349 EM----TGCPITEGYGLSET-----SPVATCNPVDatefSGTIGLPLPSTEVSIRDDDGNdlplgepgeiCIRGPQ---- 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 465 VILDSW--PGQ-ARTLYGDhdrfvdTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPK 541
Cdd:PRK07059 416 VMAGYWnrPDEtAKVMTAD------GFFRT-------GDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPG 482
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 542 VAEAAVVGVPHDIKGQGIYVYVTlngGEEP--TEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK07059 483 VLEVAAVGVPDEHSGEAVKLFVV---KKDPalTEE---DVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-616 |
2.44e-19 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 90.03 E-value: 2.44e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVfDYRPGEI-------YWCtadVGWVTGhtyiVYGPLANGATTL 328
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGERL-GLTEQDRlcipvplFHC---FGSVLG----VLACLTHGATMV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 329 LFEgvPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEgaDGSSLRLLGSVGEPINPEAWDWYYKNVGQSRCP 408
Cdd:cd05917 74 FPS--PSF-DPLAVLEAIEKEKCTALHGVPTMFIAELEHPDFDKF--DLSSLRTGIMAGAPCPPELMKRVIEVMNMKDVT 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 409 IVdtWWQTETG-GNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPAE-------GNLVILDSWPGQART-LYG 479
Cdd:cd05917 149 IA--YGMTETSpVSTQTRTDDSIEKRVNTVGRIMPHTEAKIVDPEGGIVPPVGVpgelcirGYSVMKGYWNDPEKTaEAI 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 480 DHDRFvdtyfktfrgmYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGI 559
Cdd:cd05917 227 DGDGW-----------LHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEV 295
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 560 YVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05917 296 CAWIRLKEGAELTEE---DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
106-619 |
3.57e-19 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 90.85 E-value: 3.57e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRgQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05909 6 TSLTYRKLLTGAIALARKLA-KMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEGIRAGKKIPLKanvdDALTNPETSSIQKVIVCKRTNGAIKWNQHRDIWYEDLMKVAGavCAPKEmgAEEALFILYTS 265
Cdd:cd05909 85 SKQFIEKLKLHHLF----DVEYDARIVYLEDLRAKISKADKCKAFLAGKFPPKWLLRIFG--VAPVQ--PDDPAVILFTS 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGYL--LYAALTHervFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFegvPNYPDITRVA 343
Cdd:cd05909 157 GSEGLPKGVVLSHKNLLanVEQITAI---FDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFH---PNPLDYKKIP 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 344 KIVDKHKVNILYTAPTAIRAMmasgTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTEtggnmM 423
Cdd:cd05909 231 ELIYDKKATILLGTPTFLRGY----ARAAHPEDFSSLRLVVAGAEKLKDTLRQEFQEKFG---IRILEGYGTTE-----C 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 424 SPL-----PGAHAlKPGSAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSwPGQARTLYGDHDRfvdTYFKTFRGMYFT 498
Cdd:cd05909 299 SPVisvntPQSPN-KEGTVGRPLPGMEVKIVSVETHEEVPIGEGGLLLVRG-PNVMLGYLNEPEL---TSFAFGDGWYDT 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIES-AMVAHPKVAEAAVVGVPHDIKGQGIYVYVTlnggeePTEALRL 577
Cdd:cd05909 374 GDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDiLSEILPEDNEVAVVSVPDGRKGEKIVLLTT------TTDTDPS 447
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 734849183 578 ELKNWVRK-EIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd05909 448 SLNDILKNaGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
90-619 |
4.22e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 91.16 E-value: 4.22e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 90 GDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPE 169
Cdd:PRK08162 32 PDRPAVIHGD------RRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 170 ALAGRIIDCKSKVVIT-------ADEGIR-AGKKIPLKANVDDaltnPETSSIQKVivckrtngaikwnqhRDIWYEDLM 241
Cdd:PRK08162 106 SIAFMLRHGEAKVLIVdtefaevAREALAlLPGPKPLVIDVDD----PEYPGGRFI---------------GALDYEAFL 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 242 KvAGAVCAPKEMGAEE--ALFILYTSGSTGKPKGV-QHTTGGYLlyAALTHERVFDYRPGEIY-W------CTadvGWvt 311
Cdd:PRK08162 167 A-SGDPDFAWTLPADEwdAIALNYTSGTTGNPKGVvYHHRGAYL--NALSNILAWGMPKHPVYlWtlpmfhCN---GW-- 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 312 GHTYIVygpLANGATTLLFEGVpnypDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGsslRLLGSVGEPIN 391
Cdd:PRK08162 239 CFPWTV---AARAGTNVCLRKV----DPKLIFDLIREHGVTHYCGAPIVLSALINAPAEWRAGIDH---PVHAMVAGAAP 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 392 PEAWDWYYKNVGQSrcpIVDTWWQTETGGNMMSPL--PGAHALKPGSAAR-------PFFGVVPALVDNLGNLIEGPAE- 461
Cdd:PRK08162 309 PAAVIAKMEEIGFD---LTHVYGLTETYGPATVCAwqPEWDALPLDERAQlkarqgvRYPLQEGVTVLDPDTMQPVPADg 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 462 ---------GNLVIldswpgqartlygdhdrfvDTYFK-------TFRGMYF-TGDGARRDEDGYYWITGRVDDVLNVSG 524
Cdd:PRK08162 386 etigeimfrGNIVM-------------------KGYLKnpkateeAFAGGWFhTGDLAVLHPDGYIKIKDRSKDIIISGG 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 525 HRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPgLPK 604
Cdd:PRK08162 447 ENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLAGFKVPKAVVFGE-LPK 522
|
570
....*....|....*
gi 734849183 605 TRSGKIMRRILRKIA 619
Cdd:PRK08162 523 TSTGKIQKFVLREQA 537
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
104-619 |
6.87e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 90.22 E-value: 6.87e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDvHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:PRK07638 23 NDRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDELKERLAISNADMI 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITadegiragkkiplkanvDDALTNPETSSIQKVIVCKrtngaiKWNQHRDIWYEDLMKVAGAVCAPKEMGaeealfilY 263
Cdd:PRK07638 102 VT-----------------ERYKLNDLPDEEGRVIEID------EWKRMIEKYLPTYAPIENVQNAPFYMG--------F 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLlyaaltheRVFDYRPGEIYWCTADVGWVTG---HTYIVYGPLAN---GAT-TLLFEGVPNy 336
Cdd:PRK07638 151 TSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLFLYGAISTlyvGQTvHLMRKFIPN- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 pditRVAKIVDKHKVNILYTAPTAIRAMMA-------SGTAACEGADGSSlrllGSVGE--PINPEA--WDWYyknvGQS 405
Cdd:PRK07638 222 ----QVLDKLETENISVMYTVPTMLESLYKenrvienKMKIISSGAKWEA----EAKEKikNIFPYAklYEFY----GAS 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 406 RCPIVDtwwqtetggnmmSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEgpaegnlvildswPGQARTLYGDHDRFV 485
Cdd:PRK07638 290 ELSFVT------------ALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQ-------------KGEIGTVYVKSPQFF 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 486 DTYF--------KTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ 557
Cdd:PRK07638 345 MGYIiggvlareLNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAVDEIVVIGVPDSYWGE 424
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 734849183 558 GIYVYVTLNggeepteALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK07638 425 KPVAIIKGS-------ATKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
261-612 |
1.82e-18 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 87.17 E-value: 1.82e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGVQHTTGGYLLYAAlthervfdyrpgeiYWCtaDVGWVT-GHTYIVYGPLAN------GATTLLFEGV 333
Cdd:cd17638 5 IMFTSGTTGRSKGVMCAHRQTLRAAA--------------AWA--DCADLTeDDRYLIINPFFHtfgykaGIVACLLTGA 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 334 PNYP----DITRVAKIVDKHKVNILYTAPTAIRAMMasGTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpI 409
Cdd:cd17638 69 TVVPvavfDVDAILEAIERERITVLPGPPTLFQSLL--DHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFET--V 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 410 VDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGPaegNLVI--LDSWPGQARTLYGDhdrfvdt 487
Cdd:cd17638 145 LTAYGLTEAGVATMCRPGDDAETVATTCGRACPGFEVRIADDGEVLVRGY---NVMQgyLDDPEATAEAIDAD------- 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 488 yfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNG 567
Cdd:cd17638 215 ------GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVARP 288
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 734849183 568 GEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17638 289 GVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
237-645 |
5.44e-18 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 87.77 E-value: 5.44e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 237 YEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPGEIYWCTADV--------G 308
Cdd:PRK13388 131 YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTER-FGLTRDDVCYVSMPLfhsnavmaG 209
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 309 WvtghtyivyGP-LANGATTLL---FEGVPNYPDITRVAKI----VDKHKVNILYTAptairammasgtaacEGADGSSL 380
Cdd:PRK13388 210 W---------APaVASGAAVALpakFSASGFLDDVRRYGATyfnyVGKPLAYILATP---------------ERPDDADN 265
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 381 RLLGSVGEPINPEAWDWYYKNVGqsrCPIVDTWWQTETGGNMMSPlPGAhalKPGSAARPFFGVV-----------PALV 449
Cdd:PRK13388 266 PLRVAFGNEASPRDIAEFSRRFG---CQVEDGYGSSEGAVIVVRE-PGT---PPGSIGRGAPGVAiynpetltecaVARF 338
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 450 DNLGNLIEG-PAEGNLVILDSwPGQARTLYGDHDRFVDtyfKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMG 528
Cdd:PRK13388 339 DAHGALLNAdEAIGELVNTAG-AGFFEGYYNNPEATAE---RMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLS 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 529 TAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrlELKNWV--RKEIGPIASPDVIQWAPGLPKTR 606
Cdd:PRK13388 415 AAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLRDGATFDPD---AFAAFLaaQPDLGTKAWPRYVRIAADLPSTA 491
|
410 420 430 440
....*....|....*....|....*....|....*....|....*..
gi 734849183 607 SGKIMRRILRK----IATGEY----DGLGDISTLADPGVVAHLIETH 645
Cdd:PRK13388 492 TNKVLKRELIAqgwaTGDPVTlwvrRGGPAYRLMSEPAKAALAAEFA 538
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
91-615 |
7.87e-18 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 86.45 E-value: 7.87e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEA 170
Cdd:cd17645 13 DHVAVVDRG------QSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGER 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 LAGRIIDCKSKVVITadegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcap 250
Cdd:cd17645 87 IAYMLADSSAKILLT----------------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 251 kemGAEEALFILYTSGSTGKPKGV--QHTTggyLLYAALTHERVFDYRPGEIYWCTADVGWvTGHTYIVYGPLANGATTL 328
Cdd:cd17645 102 ---NPDDLAYVIYTSGSTGLPKGVmiEHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPHLTAGAALH 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 329 LFEGVPNYpDITRVAKIVDKHKVNILYTAPTAIRAMMAsgtaacegADGSSLRLLGSVGEPINPEAWDWY--YKNVGQSR 406
Cdd:cd17645 175 VVPSERRL-DLDALNDYFNQEGITISFLPTGAAEQFMQ--------LDNQSLRVLLTGGDKLKKIERKGYklVNNYGPTE 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 407 CPIVDTwwqtetggnmMSPLPGAHALKPgsAARPFFGVVPALVDNLGNLIEGPAEGNLVILDSwpGQARTLYGDHD---- 482
Cdd:cd17645 246 NTVVAT----------SFEIDKPYANIP--IGKPIDNTRVYILDEALQLQPIGVAGELCIAGE--GLARGYLNRPEltae 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 483 RFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVY 562
Cdd:cd17645 312 KFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGRKYLVAY 391
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 734849183 563 VTLnggeePTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17645 392 VTA-----PEEIPHEELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
498-616 |
1.06e-17 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 86.65 E-value: 1.06e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNggeepTEAL-R 576
Cdd:PRK08974 436 TGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFVVKK-----DPSLtE 510
|
90 100 110 120
....*....|....*....|....*....|....*....|
gi 734849183 577 LELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK08974 511 EELITHCRRHLTGYKVPKLVEFRDELPKSNVGKILRRELR 550
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
82-551 |
1.62e-17 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 86.47 E-value: 1.62e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGddpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK08279 43 FEEAAARHPDRPALLFED------QSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADE------GIRAGKKIPLKANVDDALTNPETSSiqkvivckrtngaikwnqhrdi 235
Cdd:PRK08279 117 LNTQQRGAVLAHSLNLVDAKHLIVGEElveafeEARADLARPPRLWVAGGDTLDDPEG---------------------- 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 236 wYEDLMKVAGAVCAP-----KEMGAEEALFILYTSGSTGKPKGvqhttggyllyAALTHERVF----------DYRPGEI 300
Cdd:PRK08279 175 -YEDLAAAAAGAPTTnpasrSGVTAKDTAFYIYTSGTTGLPKA-----------AVMSHMRWLkamggfggllRLTPDDV 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 301 YWCTADVGWVTGHTYIVYGPLANGATTLL---FEGVPNYPDITRvakivdkHKVnilyTAPTAI----RAMMASgtAACE 373
Cdd:PRK08279 243 LYCCLPLYHNTGGTVAWSSVLAAGATLALrrkFSASRFWDDVRR-------YRA----TAFQYIgelcRYLLNQ--PPKP 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 374 GADGSSLRLLgsVGEPINPEAWDWYYKNVGQSRcpIVDTWWQTEtgGNMmsplpgahAL-----KPGSAAR-PFFGVVP- 446
Cdd:PRK08279 310 TDRDHRLRLM--IGNGLRPDIWDEFQQRFGIPR--ILEFYAASE--GNV--------GFinvfnFDGTVGRvPLWLAHPy 375
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 447 ALV-----------DNLGNLIE-GPAE-GNLV--ILDSWP--GqartlYGD---------HDRFV--DTYFKtfrgmyfT 498
Cdd:PRK08279 376 AIVkydvdtgepvrDADGRCIKvKPGEvGLLIgrITDRGPfdG-----YTDpeasekkilRDVFKkgDAWFN-------T 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 734849183 499 GDGARRDEDGYYWITGRVDDVL-----NVSghrmgTAEIESAMVAHPKVAEAAVVGVP 551
Cdd:PRK08279 444 GDLMRDDGFGHAQFVDRLGDTFrwkgeNVA-----TTEVENALSGFPGVEEAVVYGVE 496
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
101-617 |
2.84e-17 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 85.42 E-value: 2.84e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAihsvVFGGFSPEALAGRIidcKS 180
Cdd:PLN02330 49 EAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGG----VFSGANPTALESEI---KK 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 181 KVVITADEGIragkkiplkanVDDALTNPETSSIQ-KVIVCKRT--NGAIKWNqhrdiwyeDLMKVA---GAVCAPKEMG 254
Cdd:PLN02330 122 QAEAAGAKLI-----------VTNDTNYGKVKGLGlPVIVLGEEkiEGAVNWK--------ELLEAAdraGDTSDNEEIL 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 255 AEEALFILYTSGSTGKPKGVQHTTGGylLYAALThERVFDYRPgEIYWCTADVGWVTghTYIVYGPLANGATTLLFEG-- 332
Cdd:PLN02330 183 QTDLCALPFSSGTTGISKGVMLTHRN--LVANLC-SSLFSVGP-EMIGQVVTLGLIP--FFHIYGITGICCATLRNKGkv 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 -VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRL--LGSVGEPINPE---AWDWYYKNVG-QS 405
Cdd:PLN02330 257 vVMSRFELRTFLNALITQEVSFAPIVPPIILNLVKN--PIVEEFDLSKLKLqaIMTAAAPLAPElltAFEAKFPGVQvQE 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 406 RCPIVDTWWQTETGGNmmsPLPGAHALKPGSaarpffgvVPALVDNLGNLIEGPAEGnLVILDSWPGQ--ART---LYG- 479
Cdd:PLN02330 335 AYGLTEHSCITLTHGD---PEKGHGIAKKNS--------VGFILPNLEVKFIDPDTG-RSLPKNTPGElcVRSqcvMQGy 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 480 -----DHDRFVDTyfktfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDI 554
Cdd:PLN02330 403 ynnkeETDRTIDE-----DGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEE 477
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 734849183 555 KGQGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PLN02330 478 AGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
82-613 |
3.60e-17 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 84.94 E-value: 3.60e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEGDDPAesrtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsV 161
Cdd:PRK05852 22 VEVAATRLPEAPALVVTADRIA----ISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV--V 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VfggfsPealagriIDCKSKVVITADEGIRAGKKIPLKANVDDALTNPETSSIQKVIV---CKRTNGAIKWNQHRDIWYE 238
Cdd:PRK05852 96 V-----P-------LDPALPIAEQRVRSQAAGARVVLIDADGPHDRAEPTTRWWPLTVnvgGDSGPSGGTLSVHLDAATE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAgavcAPKEMGAEEALfILYTSGSTGKPKGVQHTTGGYllyAALTHERVFDYRPGEIYWCTADVGWVTGHTYI-- 316
Cdd:PRK05852 164 PTPATS----TPEGLRPDDAM-IMFTGGTTGLPKMVPWTHANI---ASSVRAIITGYRLSPRDATVAVMPLYHGHGLIaa 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 317 VYGPLANGATTLL-----FEGVPNYPDITRVAKIvdkhkvniLYTA-PTAIRAMMASGTAACEGADGSSLRLLGSVGEPI 390
Cdd:PRK05852 236 LLATLASGGAVLLpargrFSAHTFWDDIKAVGAT--------WYTAvPTIHQILLERAATEPSGRKPAALRFIRSCSAPL 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 391 NPEAWDWYYKNVGqsrCPIVDTWWQTET---------GGNMMSPLPGAHALKPGSAARPFFGVVPALVDNLGnliegPAE 461
Cdd:PRK05852 308 TAETAQALQTEFA---APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRSTGAQIRIVGSDGLPLP-----AGA 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 462 GNLVILdSWPGQARTLYGDHD----RFVDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMV 537
Cdd:PRK05852 380 VGEVWL-RGTTVVRGYLGDPTitaaNFTDGWLRT-------GDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLA 451
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 538 AHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTealRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRR 613
Cdd:PRK05852 452 SHPNVMEAAVFGVPDQLYGEAVAAVIVPRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
105-615 |
1.03e-16 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 84.83 E-value: 1.03e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 105 SRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVI 184
Cdd:PRK12467 1597 EQELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLL 1676
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 185 TADEgirAGKKIPLKANVDDALTNPEtssiqkvivckrtngaikwnqhrDIWYEDLMKVAGAVcapkEMGAEEALFILYT 264
Cdd:PRK12467 1677 TQSH---LQARLPLPDGLRSLVLDQE-----------------------DDWLEGYSDSNPAV----NLAPQNLAYVIYT 1726
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 265 SGSTGKPKGVQHTTGGYLlyaaltheRVFDYRpGEIYWCTADVGWVTGHTYI-------VYGPLANGATTLLFE-GVPNY 336
Cdd:PRK12467 1727 SGSTGRPKGAGNRHGALV--------NRLCAT-QEAYQLSAADVVLQFTSFAfdvsvweLFWPLINGARLVIAPpGAHRD 1797
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 PDitRVAKIVDKHKVNILYTAPTAIRAM--MASGTAACegadgSSLRLLGSVGEPINPEAWDWYYKNVGQSRcpIVDTWW 414
Cdd:PRK12467 1798 PE--QLIQLIERQQVTTLHFVPSMLQQLlqMDEQVEHP-----LSLRRVVCGGEALEVEALRPWLERLPDTG--LFNLYG 1868
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 415 QTETGGNMMSpLPGAHALKPGSAARPffgvVPALVDNLGNLIEgPAEGNLVIldswPGQARTLY--------GDH----- 481
Cdd:PRK12467 1869 PTETAVDVTH-WTCRRKDLEGRDSVP----IGQPIANLSTYIL-DASLNPVP----IGVAGELYlggvglarGYLnrpal 1938
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 --DRFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVvgVPHD-IKGQ 557
Cdd:PRK12467 1939 taERFVADPFGTVGSrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLREQGGVREAVV--IAQDgANGK 2016
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 734849183 558 GIYVYVTLNGGEEPTE-----ALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK12467 2017 QLVAYVVPTDPGLVDDdeaqvALRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDRKAL 2079
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
260-615 |
1.10e-16 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 84.83 E-value: 1.10e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 260 FILYTSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPG--EIYWCTADVGWVTGHTYIvygPLANGATTLLfeGVPNYP 337
Cdd:PRK05691 2337 YLIYTSGSTGKPKGVVVSHGEIAMHCQAVIER-FGMRADdcELHFYSINFDAASERLLV---PLLCGARVVL--RAQGQW 2410
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 338 DITRVAKIVDKHKVNILYTAPTairamMASGTAACEGADGSSL--RLLGSVGEPINPEAWdwyyKNVGQSRCP--IVDTW 413
Cdd:PRK05691 2411 GAEEICQLIREQQVSILGFTPS-----YGSQLAQWLAGQGEQLpvRMCITGGEALTGEHL----QRIRQAFAPqlFFNAY 2481
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 414 WQTETggnMMSPL--PGAHALKPGSAARPFFGVVPALV-----DNLGNLIEGpAEGNLVIldswpGQARTLYGDHD---- 482
Cdd:PRK05691 2482 GPTET---VVMPLacLAPEQLEEGAASVPIGRVVGARVayildADLALVPQG-ATGELYV-----GGAGLAQGYHDrpgl 2552
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 483 ---RFVDTYFKTFRG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAV--VGVPHDIKG 556
Cdd:PRK05691 2553 taeRFVADPFAADGGrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVlaLDTPSGKQL 2632
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 557 QGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK05691 2633 AGYLVSAVAGQDDEAQAALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
100-619 |
1.21e-16 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 83.33 E-value: 1.21e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 100 DDPAESR---TITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRI 175
Cdd:PRK12492 39 DRPAFSNlgvTLSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQF 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 176 IDCKSKVVI---------------TADEGIRAGKKIPLKANVDDALTNPETSSIQKVIVCKRTNGAIKWNQHrdiwyedL 240
Cdd:PRK12492 119 KDSGARALVylnmfgklvqevlpdTGIEYLIEAKMGDLLPAAKGWLVNTVVDKVKKMVPAYHLPQAVPFKQA-------L 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 241 MKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGylLYAALThervfdyrpgEIYWCTADVGwVTGHT------ 314
Cdd:PRK12492 192 RQGRGLSLKPVPVGLDDIAVLQYTGGTTGLAKGAMLTHGN--LVANML----------QVRACLSQLG-PDGQPlmkegq 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 315 ---------YIVYGPLANgATTLLFEG-----VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSL 380
Cdd:PRK12492 259 evmiaplplYHIYAFTAN-CMCMMVSGnhnvlITNPRDIPGFIKELGKWRFSALLGLNTLFVALMDH--PGFKDLDFSAL 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 381 RLLGSVGEPI---NPEAWDwyyknvGQSRCPIVDTWWQTETggnmmSPL----PGAHALKPGSAARPFFGVVPALVDNLG 453
Cdd:PRK12492 336 KLTNSGGTALvkaTAERWE------QLTGCTIVEGYGLTET-----SPVastnPYGELARLGTVGIPVPGTALKVIDDDG 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 454 N----------LIEGPAegnlVILDSWPGQARTlygdhDRFVDTyfktfRGMYFTGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:PRK12492 405 NelplgergelCIKGPQ----VMKGYWQQPEAT-----AEALDA-----EGWFKTGDIAVIDPDGFVRIVDRKKDLIIVS 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 524 GHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTlngGEEPTEALRlELKNWVRKEIGPIASPDVIQWAPGLP 603
Cdd:PRK12492 471 GFNVYPNEIEDVVMAHPKVANCAAIGVPDERSGEAVKLFVV---ARDPGLSVE-ELKAYCKENFTGYKVPKHIVLRDSLP 546
|
570
....*....|....*.
gi 734849183 604 KTRSGKIMRRILRKIA 619
Cdd:PRK12492 547 MTPVGKILRRELRDIA 562
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
259-612 |
2.99e-16 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 80.14 E-value: 2.99e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 259 LFILYTSGSTGKPKGVQHTTGGYLlyaalthervfdyrpgEIYWCTADVGWVTGHTYI-VYGPLA-----NGATTLLFEG 332
Cdd:cd17633 3 FYIGFTSGTTGLPKAYYRSERSWI----------------ESFVCNEDLFNISGEDAIlAPGPLShslflYGAISALYLG 66
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 333 ----VPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAAcegadgSSLRLLGSVGEPINPEAwdwyYKNVGQ--SR 406
Cdd:cd17633 67 gtfiGQRKFNPKSWIRKINQYNATVIYLVPTMLQALARTLEPE------SKIKSIFSSGQKLFEST----KKKLKNifPK 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 407 CPIVDTWWQTETggNMMSPLPGAHALKPGSAARPFFGVvpalvdnlgnliegpaegNLVILDSWPGQARTLYGDHDRFVD 486
Cdd:cd17633 137 ANLIEFYGTSEL--SFITYNFNQESRPPNSVGRPFPNV------------------EIEIRNADGGEIGKIFVKSEMVFS 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 487 TY----FKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ-GIYV 561
Cdd:cd17633 197 GYvrggFSNPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGEiAVAL 276
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|.
gi 734849183 562 YVtlngGEEPTealRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17633 277 YS----GDKLT---YKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
109-621 |
3.71e-16 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 81.99 E-value: 3.71e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:PLN03102 41 TWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRS 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 GIRAGKKIPLKANVDDALTNPETSSIQKVIVCKRTNGaikwnqhRDIWYEDLMK--------VAGAVCAPKEmgaEEALF 260
Cdd:PLN03102 121 FEPLAREVLHLLSSEDSNLNLPVIFIHEIDFPKRPSS-------EELDYECLIQrgeptpslVARMFRIQDE---HDPIS 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ILYTSGSTGKPKGV--QHTtGGYLlyAALTHERVFDYRPGEIYWCTADVGWVTGHTYiVYGPLANGATTLLFEGVpNYPD 338
Cdd:PLN03102 191 LNYTSGTTADPKGVviSHR-GAYL--STLSAIIGWEMGTCPVYLWTLPMFHCNGWTF-TWGTAARGGTSVCMRHV-TAPE 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 339 ITrvaKIVDKHKVNILYTAPTAIRAMMaSGTAACEGADGSSLRLLGSVGEPinPEAwdwYYKNVGQSRCPIVDTWWQTET 418
Cdd:PLN03102 266 IY---KNIEMHNVTHMCCVPTVFNILL-KGNSLDLSPRSGPVHVLTGGSPP--PAA---LVKKVQRLGFQVMHAYGLTEA 336
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 419 GGNMM--------SPLPGAHA--LKPGSAARpFFGVVPALVDNLGNLIEGPAEGN----LVILDSwpgqarTLYGDHDRF 484
Cdd:PLN03102 337 TGPVLfcewqdewNRLPENQQmeLKARQGVS-ILGLADVDVKNKETQESVPRDGKtmgeIVIKGS------SIMKGYLKN 409
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 485 VDTYFKTFR-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYV 563
Cdd:PLN03102 410 PKATSEAFKhGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCAFV 489
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 564 TLNGGEEPTEALRLELKN-------WVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATG 621
Cdd:PLN03102 490 VLEKGETTKEDRVDKLVTrerdlieYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIAKG 554
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
106-549 |
5.70e-16 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 81.32 E-value: 5.70e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEgiragkkiplkANVDDALT-NPETSSIQKVIVCKRtNGAIKWNQHRDIWYEDLMKVAGAVCA--PKEMGA------- 255
Cdd:cd17641 90 EDE-----------EQVDKLLEiADRIPSVRYVIYCDP-RGMRKYDDPRLISFEDVVALGRALDRrdPGLYERevaagkg 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGVQHTTGGYLLYAAlTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANG--------ATT 327
Cdd:cd17641 158 EDVAVLCTTSGTTGKPKLAMLSHGNFLGHCA-AYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGfivnfpeePET 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 328 L---LFEGVPNY----P---------------DITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEG---ADG----- 377
Cdd:cd17641 237 MmedLREIGPTFvllpPrvwegiaadvrarmmDATPFKRFMFELGMKLGLRALDRGKRGRPVSLWLRLAswlADAllfrp 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 378 -------SSLRLLGSVGEPINPEAWDwYYKNVGqsrCPIVDTWWQTETGGnmMSPLPGAHALKPGSAARPFFGvVPALVD 450
Cdd:cd17641 317 lrdrlgfSRLRSAATGGAALGPDTFR-FFHAIG---VPLKQLYGQTELAG--AYTVHRDGDVDPDTVGVPFPG-TEVRID 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 451 NLGNliegpaegnlvILDSWPGQARTLYGDHDRFVDTYFKTfrGMYFTGDGARRDEDGYYWITGRVDDVLNVS-GHRMGT 529
Cdd:cd17641 390 EVGE-----------ILVRSPGVFVGYYKNPEATAEDFDED--GWLHTGDAGYFKENGHLVVIDRAKDVGTTSdGTRFSP 456
|
490 500
....*....|....*....|
gi 734849183 530 AEIESAMVAHPKVAEAAVVG 549
Cdd:cd17641 457 QFIENKLKFSPYIAEAVVLG 476
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
82-619 |
1.36e-15 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 80.03 E-value: 1.36e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAerGDQAAIIwEGDdpaesRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsV 161
Cdd:PRK10946 31 LTRHAA--SDAIAVI-CGE-----RQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--P 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPE-----ALAGRIidcKSKVVItadegirAGKKIPLKANvDDALT--NPETSSIQKVIVCkrtngaikwNQHRD 234
Cdd:PRK10946 101 VNALFSHQrselnAYASQI---EPALLI-------ADRQHALFSD-DDFLNtlVAEHSSLRVVLLL---------NDDGE 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 235 IWYEDLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGGYllyaalthervfDY---RPGEIYWCTADVGWV- 310
Cdd:PRK10946 161 HSLDDAINHPAEDFTATPSPADEVAFFQLSGGSTGTPKLIPRTHNDY------------YYsvrRSVEICGFTPQTRYLc 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 311 ---TGHTYIVYGP------LANGATTLlfegVPNyPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAACEGADGSSLR 381
Cdd:PRK10946 229 alpAAHNYPMSSPgalgvfLAGGTVVL----APD-PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLASLK 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 382 LLgsvgepinpeawdwyykNVGQSRcpivdtwwqtetggnmmsplpgahaLKPGSAARpffgvVPALvdnLGNLIE---G 458
Cdd:PRK10946 304 LL-----------------QVGGAR-------------------------LSETLARR-----IPAE---LGCQLQqvfG 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 459 PAEG--NLVILDSWPGQARTLYG------DHDRFVDT------------------YfkTFRG----------------MY 496
Cdd:PRK10946 334 MAEGlvNYTRLDDSDERIFTTQGrpmspdDEVWVADAdgnplpqgevgrlmtrgpY--TFRGyykspqhnasafdangFY 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTlnggeePTEALR 576
Cdd:PRK10946 412 CSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLV------VKEPLK 485
|
570 580 590 600
....*....|....*....|....*....|....*....|....*..
gi 734849183 577 -LELKNWVRkEIGpIAS---PDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK10946 486 aVQLRRFLR-EQG-IAEfklPDRVECVDSLPLTAVGKVDKKQLRQWL 530
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
90-641 |
1.44e-15 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 80.21 E-value: 1.44e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 90 GDQAAIIWEGDDPAEsrtITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIhsvvfggFSP 168
Cdd:PRK05620 24 GDTTVTTWGGAEQEQ---TTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAV-------FNP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 169 --EALAG----RIIDCKSKVVITADEGIrAGKKIPLKanvddaltnPETSSIQKVIVCKR---TNGAIKWNQHRDIW-YE 238
Cdd:PRK05620 94 lnKQLMNdqivHIINHAEDEVIVADPRL-AEQLGEIL---------KECPCVRAVVFIGPsdaDSAAAHMPEGIKVYsYE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 239 DLMKVAGAVCAPKEMGAEEALFILYTSGSTGKPKGVQHTTGG-YLLYAALTHERVFDYRPGE-------IY----WCTAD 306
Cdd:PRK05620 164 ALLDGRSTVYDWPELDETTAAAICYSTGTTGAPKGVVYSHRSlYLQSLSLRTTDSLAVTHGEsflccvpIYhvlsWGVPL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 307 VGWVTGhtyivygplangaTTLLFEGvpnyPDIT--RVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADGSSLRLLG 384
Cdd:PRK05620 244 AAFMSG-------------TPLVFPG----PDLSapTLAKIIATAMPRVAHGVPTLWIQLMVH--YLKNPPERMSLQEIY 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 385 SVGEPINP---EAWDWYYKnvgqsrCPIVDTWWQTETG--GNMMSPlPGAHALKPGSAARPFFGVVPA----LVDNLGNL 455
Cdd:PRK05620 305 VGGSAVPPiliKAWEERYG------VDVVHVWGMTETSpvGTVARP-PSGVSGEARWAYRVSQGRFPAsleyRIVNDGQV 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 456 IEGP--AEGNLVILDSW-------------PGQARTLYGDHDRFVDTYFkTFRGMYFTGDGARRDEDGYYWITGRVDDVL 520
Cdd:PRK05620 378 MESTdrNEGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRF-TADGWLRTGDVGSVTRDGFLTIHDRARDVI 456
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 521 NVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAP 600
Cdd:PRK05620 457 RSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVD 536
|
570 580 590 600
....*....|....*....|....*....|....*....|..
gi 734849183 601 GLPKTRSGKIMRRILRK-IATGEYdglgDISTLADPGVVAHL 641
Cdd:PRK05620 537 EIDKTSVGKFDKKDLRQhLADGDF----EIIKLKGPGESGES 574
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
235-621 |
2.69e-15 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 80.21 E-value: 2.69e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 235 IWyEDLMKVAGAVCAPKEMGAEEAL-FILYTSGSTGKPKGVQHTTGGYL-------LYAALTHERVFDYrpgeiywcTAD 306
Cdd:PRK05691 3848 VW-EEVQAGEVASHNPGIYSGPDNLaYVIYTSGSTGLPKGVMVEQRGMLnnqlskvPYLALSEADVIAQ--------TAS 3918
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 307 VGWVTGHTYIVYGPLANGATTLlfegVPNypDITR----VAKIVDKHKVNILYTAPTAIRAMMASGTAACEGadgssLRL 382
Cdd:PRK05691 3919 QSFDISVWQFLAAPLFGARVEI----VPN--AIAHdpqgLLAHVQAQGITVLESVPSLIQGMLAEDRQALDG-----LRW 3987
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 383 LGSVGEPINPE---AWDWYYKNVGqsrcpIVDTWWQTETGGNM------MSPLPGAHaLKPGSaarPFFGVVPALVDNLG 453
Cdd:PRK05691 3988 MLPTGEAMPPElarQWLQRYPQIG-----LVNAYGPAECSDDVaffrvdLASTRGSY-LPIGS---PTDNNRLYLLDEAL 4058
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 454 NLIEGPAEGNLVILDSwpGQARTLYGDHDRFVDTYFKTFRG-----MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMG 528
Cdd:PRK05691 4059 ELVPLGAVGELCVAGT--GVGRGYVGDPLRTALAFVPHPFGapgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIE 4136
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 529 TAEIESAMVAHPKVAEAAvVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSG 608
Cdd:PRK05691 4137 LGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYLVPHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANG 4215
|
410
....*....|...
gi 734849183 609 KIMRRILRKIATG 621
Cdd:PRK05691 4216 KLDRKALPALDIG 4228
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
264-617 |
3.96e-15 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 77.39 E-value: 3.96e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGYLLYAALTHERVfdyrPGEIYWCTADVGW-VTGHTYIVYGPLAnGATTLLFEgVPNYPDITRV 342
Cdd:PRK07824 43 TSGTTGTPKGAMLTAAALTASADATHDRL----GGPGQWLLALPAHhIAGLQVLVRSVIA-GSEPVELD-VSAGFDPTAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 343 AKIVDKHKVNILYTAPTAIRAMMASGTAACEGAdgssLRLLGSV---GEPINPEAWDwyykNVGQSRCPIVDTWWQTETG 419
Cdd:PRK07824 117 PRAVAELGGGRRYTSLVPMQLAKALDDPAATAA----LAELDAVlvgGGPAPAPVLD----AAAAAGINVVRTYGMSETS 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 420 GNMM---SPLPGAHALKPGSaaRPFFGvvpalvdnlgnlieGP--AEG--NLVildswpgqartlygDHDRFVDTyfktf 492
Cdd:PRK07824 189 GGCVydgVPLDGVRVRVEDG--RIALG--------------GPtlAKGyrNPV--------------DPDPFAEP----- 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 493 rGMYFTGDGARRDeDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPT 572
Cdd:PRK07824 234 -GWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGGPAPT 311
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 734849183 573 EAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07824 312 LE---ALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
82-619 |
4.47e-15 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 78.35 E-value: 4.47e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIWEgddpaeSRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PLN02479 26 LERAAVVHPTRKSVVHG------SVRYTWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNC 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEALAGRIIDCKSKVVITADE---------GIRAGK-----KIPLKANVDDALTNPEtsSIQKVIvckrTNGAI 227
Cdd:PLN02479 100 VNIRLNAPTIAFLLEHSKSEVVMVDQEfftlaeealKILAEKkkssfKPPLLIVIGDPTCDPK--SLQYAL----GKGAI 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 228 KwnqhrdiwYEDLMKVAGAVCAPKEMGAE-EALFILYTSGSTGKPKGV-QHTTGGYLLyaALTHERVFDYRPGEIY---- 301
Cdd:PLN02479 174 E--------YEKFLETGDPEFAWKPPADEwQSIALGYTSGTTASPKGVvLHHRGAYLM--ALSNALIWGMNEGAVYlwtl 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 302 -------WC-TADVGWVTGhTYI---------VYGPLANGATTLlFEGVPnypdiTRVAKIVDKHKVNILYTAPTAIRAM 364
Cdd:PLN02479 244 pmfhcngWCfTWTLAALCG-TNIclrqvtakaIYSAIANYGVTH-FCAAP-----VVLNTIVNAPKSETILPLPRVVHVM 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 365 MASgtAACEGADGSSLRLLG-------SVGEPINPE---AW--DWyyknvgqSRCPIVDTWWQTETGGNMMSPLPGAHAL 432
Cdd:PLN02479 317 TAG--AAPPPSVLFAMSEKGfrvthtyGLSETYGPStvcAWkpEW-------DSLPPEEQARLNARQGVRYIGLEGLDVV 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 433 KPGSAARpffgvVPALVDNLGNLIegpAEGNLVIldswPGQARTLYGDHDRFVDtyfktfrGMYFTGDGARRDEDGYYWI 512
Cdd:PLN02479 388 DTKTMKP-----VPADGKTMGEIV---MRGNMVM----KGYLKNPKANEEAFAN-------GWFHSGDLGVKHPDGYIEI 448
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 513 TGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRL--ELKNWVRKEIGPI 590
Cdd:PLN02479 449 KDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDEAALaeDIMKFCRERLPAY 528
|
570 580
....*....|....*....|....*....
gi 734849183 591 ASPDVIQWAPgLPKTRSGKIMRRILRKIA 619
Cdd:PLN02479 529 WVPKSVVFGP-LPKTATGKIQKHVLRAKA 556
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
108-618 |
6.98e-15 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 77.95 E-value: 6.98e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAD 187
Cdd:cd17642 45 YSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVFCSK 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EGIragKKIplkANVDDALTnpetsSIQKVIVCkrtngaikwNQHRDiwYEDLMKVAGAVCAPKEMGAEEALF------- 260
Cdd:cd17642 125 KGL---QKV---LNVQKKLK-----IIKTIIIL---------DSKED--YKGYQCLYTFITQNLPPGFNEYDFkppsfdr 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 261 ------ILYTSGSTGKPKGVQHTTGGylLYAALTHER--VFDYRPGE-------IYW-----CTADVGWVTGHTYIVYGP 320
Cdd:cd17642 183 deqvalIMNSSGSTGLPKGVQLTHKN--IVARFSHARdpIFGNQIIPdtailtvIPFhhgfgMFTTLGYLICGFRVVLMY 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 321 LANGATTLlfegvpnypditrvaKIVDKHKVNILYTAPTaIRAMMASGTAAcEGADGSSLRLLGSVGEPINPEAWDWYYK 400
Cdd:cd17642 261 KFEEELFL---------------RSLQDYKVQSALLVPT-LFAFFAKSTLV-DKYDLSNLHEIASGGAPLSKEVGEAVAK 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 401 NVGqsrCPIV-DTWWQTETGGNMMSPLPGAhaLKPGSAAR--PFFGVvpALVDNLGNLIEGPAE-GNLVIldSWPGQART 476
Cdd:cd17642 324 RFK---LPGIrQGYGLTETTSAILITPEGD--DKPGAVGKvvPFFYA--KVVDLDTGKTLGPNErGELCV--KGPMIMKG 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 477 LYGDHDRFVDTYFKTfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKG 556
Cdd:cd17642 395 YVNNPEATKALIDKD--GWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAG 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 557 QGIYVYVTLNGGEEPTEAlrlELKNWVRKEIGPiaspdvIQWAPG-------LPKTRSGKIMRRILRKI 618
Cdd:cd17642 473 ELPAAVVVLEAGKTMTEK---EVMDYVASQVST------AKRLRGgvkfvdeVPKGLTGKIDRRKIREI 532
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
256-615 |
1.84e-14 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 76.32 E-value: 1.84e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 256 EEALFILYTSGSTGKPKGV--------QHTTGGYLLYAALTHERV-------FDYRPGEIYwctadVGWVTGHTYIVygp 320
Cdd:cd17644 106 ENLAYVIYTSGSTGKPKGVmiehqslvNLSHGLIKEYGITSSDRVlqfasiaFDVAAEEIY-----VTLLSGATLVL--- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 321 langattllfegVPN--YPDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADG-SSLRLLGSVGEPINPEAWDW 397
Cdd:cd17644 178 ------------RPEemRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLELLL--STIDLpSSLRLVIVGGEAVQPELVRQ 243
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 398 YYKNVGQsRCPIVDTWWQTE-TGGNMMSPLPGAHALKPGSAA--RPFFGVVPALVDNlgNLIEGPAE--GNLVIldswpG 472
Cdd:cd17644 244 WQKNVGN-FIQLINVYGPTEaTIAATVCRLTQLTERNITSVPigRPIANTQVYILDE--NLQPVPVGvpGELHI-----G 315
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 473 QARTLYG-------DHDRFVDTYF--KTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVA 543
Cdd:cd17644 316 GVGLARGylnrpelTAEKFISHPFnsSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVK 395
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 734849183 544 EAAVVgVPHDIKGQGIYV-YVTLNGGEEP-TEALRLELKNwvRKEIGPIASPDVIqwAPGLPKTRSGKIMRRIL 615
Cdd:cd17644 396 TAVVI-VREDQPGNKRLVaYIVPHYEESPsTVELRQFLKA--KLPDYMIPSAFVV--LEELPLTPNGKIDRRAL 464
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
109-549 |
1.89e-14 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 76.35 E-value: 1.89e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITade 188
Cdd:cd05932 8 TWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV--- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 189 giraGKkipLKANVDDALTNPEtsSIQKVIVCKRTNGaikwNQHRDiwYEDLMKVAGAVCAPKEMGAEEALFILYTSGST 268
Cdd:cd05932 85 ----GK---LDDWKAMAPGVPE--GLISISLPPPSAA----NCQYQ--WDDLIAQHPPLEERPTRFPEQLATLIYTSGTT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 269 GKPKGVQHTTG--GYLLYAALTH------ERVFDYRPgeiywctadVGWVTGHTYIVYGPLANGATTLLFEGVPNYPDIT 340
Cdd:cd05932 150 GQPKGVMLTFGsfAWAAQAGIEHigteenDRMLSYLP---------LAHVTERVFVEGGSLYGGVLVAFAESLDTFVEDV 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 341 RVAK-----------------IVDK---HKVNILYTAPTaIRAMMASGTAACEGADgsSLRLLGSVGEPINPEAWDWyYK 400
Cdd:cd05932 221 QRARptlffsvprlwtkfqqgVQDKipqQKLNLLLKIPV-VNSLVKRKVLKGLGLD--QCRLAGCGSAPVPPALLEW-YR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 401 NVGqsrCPIVDTWWQTETGGNMMSPLPGAHalKPGSAARPFFGVVPALVDnlgnliegpaEGNLVILDswPGQARTLYGD 480
Cdd:cd05932 297 SLG---LNILEAYGMTENFAYSHLNYPGRD--KIGTVGNAGPGVEVRISE----------DGEILVRS--PALMMGYYKD 359
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 481 HDRFVDTYfkTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVS-GHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:cd05932 360 PEATAEAF--TADGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
402-616 |
1.96e-14 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 76.28 E-value: 1.96e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 402 VGQSRCP--------------IVDTWWQTEtggnmMSP------LPGAHALKPGSA--------ARPFFGVVPALVDNLG 453
Cdd:PRK07008 300 IGGSACPpamirtfedeygveVIHAWGMTE-----MSPlgtlckLKWKHSQLPLDEqrkllekqGRVIYGVDMKIVGDDG 374
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 454 NLI--EGPAEGNLVILDSWpgqartlygdhdrFVDTYFKT-----FRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHR 526
Cdd:PRK07008 375 RELpwDGKAFGDLQVRGPW-------------VIDRYFRGdasplVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEW 441
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 527 MGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTealRLELKNWVRKEIGPIASPDVIQWAPGLPKTR 606
Cdd:PRK07008 442 ISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGAEVT---REELLAFYEGKVAKWWIPDDVVFVDAIPHTA 518
|
250
....*....|
gi 734849183 607 SGKIMRRILR 616
Cdd:PRK07008 519 TGKLQKLKLR 528
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
106-616 |
2.22e-14 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 76.07 E-value: 2.22e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK07514 27 LRYTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVC 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEGIRAGKKIPLKANVDDALTNPEtssiqkvivcKRTNGAIKWNQHRDIWYEDlmkvagAVCAPKEMGAeealfILYTS 265
Cdd:PRK07514 107 DPANFAWLSKIAAAAGAPHVETLDA----------DGTGSLLEAAAAAPDDFET------VPRGADDLAA-----ILYTS 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGE-------IYwctadvgwvtgHTYIVY----GPLANGATTLLFegvP 334
Cdd:PRK07514 166 GTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDvlihalpIF-----------HTHGLFvatnVALLAGASMIFL---P 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 335 NYpDITRVAKIVDKHKVniLYTAPTAIRAMMAS---GTAACegadgSSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVD 411
Cdd:PRK07514 231 KF-DPDAVLALMPRATV--MMGVPTFYTRLLQEprlTREAA-----AHMRLFISGSAPLLAETHREFQERTGH---AILE 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 412 TWWQTETGGNMMSPLPGAHalKPGSAARPFFGVVPALVDN----------LGNL-IEGPaegNlVILDSW--PGQARTLY 478
Cdd:PRK07514 300 RYGMTETNMNTSNPYDGER--RAGTVGFPLPGVSLRVTDPetgaelppgeIGMIeVKGP---N-VFKGYWrmPEKTAEEF 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 GDhdrfvDTYFKTfrgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQG 558
Cdd:PRK07514 374 RA-----DGFFIT-------GDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEG 441
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 734849183 559 IYVYVTLNGGEEPTE-ALRLELKnwvrkeiGPIAS---PDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK07514 442 VTAVVVPKPGAALDEaAILAALK-------GRLARfkqPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
167-619 |
1.43e-13 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 74.19 E-value: 1.43e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 167 SPEALAGRIIDCKSKVVITADEGIRagkKIPLKANVDDALTNPETssiqkvivckrtngaikwnqhrdIWYEDLMKVAGA 246
Cdd:PRK08633 700 SEAALKSAIEQAQIKTVITSRKFLE---KLKNKGFDLELPENVKV-----------------------IYLEDLKAKISK 753
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 247 V---CA-------PKEM---------GAEEALFILYTSGSTGKPKGVQhttggyllyaaLTHER----------VFDYRP 297
Cdd:PRK08633 754 VdklTAllaarllPARLlkrlygptfKPDDTATIIFSSGSEGEPKGVM-----------LSHHNilsnieqisdVFNLRN 822
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 298 GEIYWCTADVGWVTGHTYIVYGPLANGATTLLfegVPNYPDITRVAKIVDKHKVNILYTAPTAIRAMMASgtAACEGADG 377
Cdd:PRK08633 823 DDVILSSLPFFHSFGLTVTLWLPLLEGIKVVY---HPDPTDALGIAKLVAKHRATILLGTPTFLRLYLRN--KKLHPLMF 897
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 378 SSLRLLGSVGEPINPEAWDWYYKNVGQsrcPIVDTWWQTETggnmmSP-----LPGA--------HALKPGSAARPFFGV 444
Cdd:PRK08633 898 ASLRLVVAGAEKLKPEVADAFEEKFGI---RILEGYGATET-----SPvasvnLPDVlaadfkrqTGSKEGSVGMPLPGV 969
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 445 VPALVD--NLGNLIEGpAEGNLVIldSWPGQARTLYGDHDRfVDTYFKTFRGM--YFTGDGARRDEDGYYWITGRVDdvl 520
Cdd:PRK08633 970 AVRIVDpeTFEELPPG-EDGLILI--GGPQVMKGYLGDPEK-TAEVIKDIDGIgwYVTGDKGHLDEDGFLTITDRYS--- 1042
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 521 nvsghRMgtAEIESAMVAHPKVAEA------------AVVGVPHDIKGQGIYVYVTLngGEEPTEALRLELKNwvrKEIG 588
Cdd:PRK08633 1043 -----RF--AKIGGEMVPLGAVEEElakalggeevvfAVTAVPDEKKGEKLVVLHTC--GAEDVEELKRAIKE---SGLP 1110
|
490 500 510
....*....|....*....|....*....|.
gi 734849183 589 PIASPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK08633 1111 NLWKPSRYFKVEALPLLGSGKLDLKGLKELA 1141
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
87-618 |
1.83e-13 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 73.47 E-value: 1.83e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 87 AERGDQAAIIWEGDDPAESRtITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACarigaihsvVFGGF 166
Cdd:cd05906 20 AERGPTKGITYIDADGSEEF-QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWAC---------VLAGF 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 167 SPealagriidCKSKVVITADEGIRAGKKIplkANVDDALTNPetssiqKVIVCKRTNGAI----KWNQHRDI---WYED 239
Cdd:cd05906 90 VP---------APLTVPPTYDEPNARLRKL---RHIWQLLGSP------VVLTDAELVAEFagleTLSGLPGIrvlSIEE 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 240 LMKVAGAVCAPkEMGAEEALFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIY--WCTAD--VGWVTGHTY 315
Cdd:cd05906 152 LLDTAADHDLP-QSRPDDLALLMLTSGSTGFPKAVPLTHRN-ILARSAGKIQHNGLTPQDVFlnWVPLDhvGGLVELHLR 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 316 IVYgpLA----NGATTLLFEGVPNYPDItrvakiVDKHKVNILYtAPTAIRAMMASgtAACEGADG----SSLRLLGSVG 387
Cdd:cd05906 230 AVY--LGcqqvHVPTEEILADPLRWLDL------IDRYRVTITW-APNFAFALLND--LLEEIEDGtwdlSSLRYLVNAG 298
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 388 EPINPEAWDWYYKNVGQSRCP---IVDTWWQTETGGNMM-----SPLPGAHALKPGSAARPFFGVVPALVDNLGNLIEGP 459
Cdd:cd05906 299 EAVVAKTIRRLLRLLEPYGLPpdaIRPAFGMTETCSGVIysrsfPTYDHSQALEFVSLGRPIPGVSMRIVDDEGQLLPEG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 460 AEGNLVIldswPGQARTlygdhdrfvDTYFK-------TFR--GMYFTGDGARRDeDGYYWITGRVDDVLNVSGHRMGTA 530
Cdd:cd05906 379 EVGRLQV----RGPVVT---------KGYYNnpeanaeAFTedGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSH 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 531 EIESAMVAHPKVAEAAVVGVPHDIKGQG----IYVYVTLNGGEEPTEALRLELKNWVRKEIGpIASPDVIQWAPG-LPKT 605
Cdd:cd05906 445 EIEAAVEEVPGVEPSFTAAFAVRDPGAEteelAIFFVPEYDLQDALSETLRAIRSVVSREVG-VSPAYLIPLPKEeIPKT 523
|
570
....*....|...
gi 734849183 606 RSGKIMRRILRKI 618
Cdd:cd05906 524 SLGKIQRSKLKAA 536
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
104-612 |
3.72e-13 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 72.09 E-value: 3.72e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd05914 4 GGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAI 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITADEgiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyEDLMkvagavcapkemgaeealFILY 263
Cdd:cd05914 84 FVSDE-------------------------------------------------DDVA------------------LINY 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQhTTGGYLLYAALTHERVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLLFE------------ 331
Cdd:cd05914 97 TSGTTGNSKGVM-LTYRNIVSNVDGVKEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDkipsakiialaf 175
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 332 -------GVPNYPDITRVAKIVDKHKVNI------LYTAPTAIRAMMASGTAACEGAdGSSLRLLGSVGEPINPEAWDWY 398
Cdd:cd05914 176 aqvtptlGVPVPLVIEKIFKMDIIPKLTLkkfkfkLAKKINNRKIRKLAFKKVHEAF-GGNIKEFVIGGAKINPDVEEFL 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 399 YKnvgqSRCPIVDTWWQTETGGNMMSPLPGahALKPGSAARPFFGVVPALVDnlgnliEGPAEGNLVILDSWPGQARTLY 478
Cdd:cd05914 255 RT----IGFPYTIGYGMTETAPIISYSPPN--RIRLGSAGKVIDGVEVRIDS------PDPATGEGEIIVRGPNVMKGYY 322
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 479 GDHDRFVDTYFKTfrGMYFTGDGARRDEDGYYWITGRVDDV-LNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQ 557
Cdd:cd05914 323 KNPEATAEAFDKD--GWFHTGDLGKIDAEGYLYIRGRKKEMiVLSSGKNIYPEEIEAKINNMPFVLESLVVVQEKKLVAL 400
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 558 GIYVYVTLNGGEEPTEALRLELKNWVRKEIGPIASP-----DVIQWAPGLPKTRSGKIMR 612
Cdd:cd05914 401 AYIDPDFLDVKALKQRNIIDAIKWEVRDKVNQKVPNykkisKVKIVKEEFEKTPKGKIKR 460
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
494-615 |
4.44e-13 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 71.95 E-value: 4.44e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 494 GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTE 573
Cdd:PRK13383 396 GMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVDA 475
|
90 100 110 120
....*....|....*....|....*....|....*....|..
gi 734849183 574 AlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK13383 476 A---QLRDYLKDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
82-622 |
2.23e-12 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 69.84 E-value: 2.23e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 82 LDRHLAERGDQAAIIwegdDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSV 161
Cdd:PRK08315 22 LDRTAARYPDREALV----YRDQGLRWTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAILVT 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 162 VFGGFSPEAL--AGRIIDCKskVVITAD--------EGIRAgkKIP-LKANVDDALTNPETSSIQKVIVC--KRTNGAIK 228
Cdd:PRK08315 98 INPAYRLSELeyALNQSGCK--ALIAADgfkdsdyvAMLYE--LAPeLATCEPGQLQSARLPELRRVIFLgdEKHPGMLN 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 229 WNqhrdiwyeDLMKVAGAVCAPK--EMGAE----EALFILYTSGSTGKPKGVQHT-----TGGYLLYAA--LTHErvfDy 295
Cdd:PRK08315 174 FD--------ELLALGRAVDDAElaARQATldpdDPINIQYTSGTTGFPKGATLThrnilNNGYFIGEAmkLTEE---D- 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 296 R---PGEIYWCtadVGWVTGhtyiVYGPLANGATTLlfegvpnYP----DITRVAKIVDKHKVNILYTAPTAIRAMMASG 368
Cdd:PRK08315 242 RlciPVPLYHC---FGMVLG----NLACVTHGATMV-------YPgegfDPLATLAAVEEERCTALYGVPTMFIAELDHP 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 369 TAAceGADGSSLR---LLGSVgepinpeawdwyyknvgqsrCPI------VD---------TWWQTETG-GNMMS----P 425
Cdd:PRK08315 308 DFA--RFDLSSLRtgiMAGSP--------------------CPIevmkrvIDkmhmsevtiAYGMTETSpVSTQTrtddP 365
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 426 LpgahALKPGSAARPFFGVVPALVDNLGNLIEGPAE-------GNLVILDSWPGQARTLYG-DHDRFvdtyfktfrgMYf 497
Cdd:PRK08315 366 L----EKRVTTVGRALPHLEVKIVDPETGETVPRGEqgelctrGYSVMKGYWNDPEKTAEAiDADGW----------MH- 430
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEAlrl 577
Cdd:PRK08315 431 TGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTEE--- 507
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 734849183 578 ELKNWVRkeiGPIAS---PDVIQWAPGLPKTRSGKIMRRILRKIATGE 622
Cdd:PRK08315 508 DVRDFCR---GKIAHykiPRYIRFVDEFPMTVTGKIQKFKMREMMIEE 552
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
106-617 |
5.69e-12 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 68.22 E-value: 5.69e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05939 2 RHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 adegiragkkiplkaNVDDALTNpETSSIQKVIVCKrtngaikwnQHRDIwyedlmkvagavcapkemgaeeaLFILYTS 265
Cdd:cd05939 82 ---------------NLLDPLLT-QSSTEPPSQDDV---------NFRDK-----------------------LFYIYTS 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKGVQHTTGGYLLYAALTHeRVFDYRPGEIYWCTADVGWVTGHTYIVYGPLANGATTLL---FEGVPNYPDitrv 342
Cdd:cd05939 114 GTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHGSTVVIrkkFSASNFWDD---- 188
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 343 akiVDKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLgsVGEPINPEAWDWYYKNVGQSRcpIVDTWWQTETGGNM 422
Cdd:cd05939 189 ---CVKYNCTIVQYIGEICRYLLA--QPPSEEEQKHNVRLA--VGNGLRPQIWEQFVRRFGIPQ--IGEFYGATEGNSSL 259
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 423 MSplpgaHALKPGsaARPFFGVVPALV---------DNLGNLIEGPaegNLVILDSWPGQARTLYG-----DHDRFVDTY 488
Cdd:cd05939 260 VN-----IDNHVG--ACGFNSRILPSVypirlikvdEDTGELIRDS---DGLCIPCQPGEPGLLVGkiiqnDPLRRFDGY 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 489 --------------FKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGV--PH 552
Cdd:cd05939 330 vnegatnkkiardvFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLEDVVVYGVevPG 409
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 734849183 553 DIKGQGIYVYVtlnggeEPTEALRLE-LKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05939 410 VEGRAGMAAIV------DPERKVDLDrFSAVLAKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQK 469
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
259-608 |
1.70e-11 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 65.79 E-value: 1.70e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 259 LFILYTSGSTGKPKGVQHTTGGyLLYAALTHERVFDYRPGEIYWctadvgwVTGHTYIVyGPLANGATTLLFEG----VP 334
Cdd:cd17636 3 VLAIYTAAFSGRPNGALLSHQA-LLAQALVLAVLQAIDEGTVFL-------NSGPLFHI-GTLMFTLATFHAGGtnvfVR 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 335 NYpDITRVAKIVDKHKVNILYTAPTAIRAMMASGTAacEGADGSSLRLLGSVGE---PINPEAWDWYYKNVGQSrcpivd 411
Cdd:cd17636 74 RV-DAEEVLELIEAERCTHAFLLPPTIDQIVELNAD--GLYDLSSLRSSPAAPEwndMATVDTSPWGRKPGGYG------ 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 412 twwQTETGGNMMSPLPGAHALkpGSAARPFFGVVPALVDNLGNLI------EGPAEGNLVILDSW--PG--QARTlygdh 481
Cdd:cd17636 145 ---QTEVMGLATFAALGGGAI--GGAGRPSPLVQVRILDEDGREVpdgevgEIVARGPTVMAGYWnrPEvnARRT----- 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 dRFvdtyfktfrGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYV 561
Cdd:cd17636 215 -RG---------GWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPRWAQSVKA 284
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 734849183 562 YVTLNGGEEPTEAlrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSG 608
Cdd:cd17636 285 IVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
104-617 |
5.58e-11 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 65.15 E-value: 5.58e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05937 2 EGKTWTYSETYDLVLRYAHWLHDDlGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 VITADEGIRAgkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgaeealfIL 262
Cdd:cd05937 82 VIVDPDDPAI--------------------------------------------------------------------LI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGVQHTT-----GGYLL--YAALTHE-RVFDYRPgeIYWCTADVgwvTGHTYIvygpLANGATTLL---FE 331
Cdd:cd05937 94 YTSGTTGLPKAAAISWrrtlvTSNLLshDLNLKNGdRTYTCMP--LYHGTAAF---LGACNC----LMSGGTLALsrkFS 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 332 GVPNYPDItrvakiVDKHKVNILYTAPTaIRAMMASGTA------ACEGADGSSLRllgsvgepinPEAWDWYYKNVGqs 405
Cdd:cd05937 165 ASQFWKDV------RDSGATIIQYVGEL-CRYLLSTPPSpydrdhKVRVAWGNGLR----------PDIWERFRERFN-- 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 406 rCPIVDTWWQ-TETGGNMMSPLPGAHALKP----GSAARPFFG--VVPALVD-NLGNLIEGPAEGNLV---------ILD 468
Cdd:cd05937 226 -VPEIGEFYAaTEGVFALTNHNVGDFGAGAighhGLIRRWKFEnqVVLVKMDpETDDPIRDPKTGFCVrapvgepgeMLG 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 469 SWPGQARTL----YGDHD----RFVDTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHP 540
Cdd:cd05937 305 RVPFKNREAfqgyLHNEDatesKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHP 384
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 541 KVAEAAVVGVP---HDikGQGIYVYVTL-NGGEEPTEALRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05937 385 DIAEANVYGVKvpgHD--GRAGCAAITLeESSAVPTEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLR 462
|
.
gi 734849183 617 K 617
Cdd:cd05937 463 D 463
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
456-620 |
1.05e-10 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 64.25 E-value: 1.05e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 456 IEGPAEGNLVIldswpgQARTLY-GDHDRFVDTyfktfRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIES 534
Cdd:PRK07445 296 IPANQTGNITI------QAQSLAlGYYPQILDS-----QGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEA 364
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 535 AMVAHPKVAEAAVVGVPHDIKGQ---GIYVYVTLNGGEEptealrlELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIM 611
Cdd:PRK07445 365 AILATGLVQDVCVLGLPDPHWGEvvtAIYVPKDPSISLE-------ELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKIN 437
|
....*....
gi 734849183 612 RRILRKIAT 620
Cdd:PRK07445 438 RQQLQQIAV 446
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
84-395 |
2.98e-10 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 62.99 E-value: 2.98e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 84 RHL----AERGDQAAII----WEGDDPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARI 155
Cdd:PRK09274 10 RHLpraaQERPDQLAVAvpggRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 156 GAIHSVVFGGFSPEALAGRIIDCKSKVVITadegiragkkIPlKANVDDALTNPETSSIQKVIVCKRtngaikwnqhRDI 235
Cdd:PRK09274 90 GAVPVLVDPGMGIKNLKQCLAEAQPDAFIG----------IP-KAHLARRLFGWGKPSVRRLVTVGG----------RLL 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 236 W----YEDLMKVAG------AVCAPKEMGAeealfILYTSGSTGKPKGVQHTTGGYLlyAALTHER-VFDYRPGEIywct 304
Cdd:PRK09274 149 WggttLATLLRDGAaapfpmADLAPDDMAA-----ILFTSGSTGTPKGVVYTHGMFE--AQIEALReDYGIEPGEI---- 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 305 aDVgwvtgHTYIVYG--PLANGATTLLfegvpnyPDI--TRVA-----KIVD---KHKVNILYTAPTAIRAMmasgTAAC 372
Cdd:PRK09274 218 -DL-----PTFPLFAlfGPALGMTSVI-------PDMdpTRPAtvdpaKLFAaieRYGVTNLFGSPALLERL----GRYG 280
|
330 340
....*....|....*....|....*
gi 734849183 373 EGADGS--SLRLLGSVGEPINPEAW 395
Cdd:PRK09274 281 EANGIKlpSLRRVISAGAPVPIAVI 305
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
497-615 |
3.47e-10 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 62.36 E-value: 3.47e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGGEEPtealr 576
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEIDP----- 368
|
90 100 110
....*....|....*....|....*....|....*....
gi 734849183 577 LELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK08308 369 VQLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
255-580 |
6.50e-10 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 61.71 E-value: 6.50e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 255 AEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERvFDYRPGEiywctadVGWVTGHTYIVYGPlANGATTLLFEGVP 334
Cdd:cd05910 84 ADEPAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQL-YGIRPGE-------VDLATFPLFALFGP-ALGLTSVIPDMDP 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 335 NYPDITRVAKIVD---KHKVNILYTAPtairAMMASGTAACE--GADGSSLRLLGSVGEPINPEAWDWYYKNVGQSrCPI 409
Cdd:cd05910 155 TRPARADPQKLVGairQYGVSIVFGSP----ALLERVARYCAqhGITLPSLRRVLSAGAPVPIALAARLRKMLSDE-AEI 229
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 410 VDTWWQTETggnmmspLP----GAHALKPGSAA-----------RPFFGVVPALVD-------NLGNLIEGPAEGNLVIL 467
Cdd:cd05910 230 LTPYGATEA-------LPvssiGSRELLATTTAatsggagtcvgRPIPGVRVRIIEiddepiaEWDDTLELPRGEIGEIT 302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 468 DSWPGQARTLYG--DHDRF--VDTYFKTFRgmYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVA 543
Cdd:cd05910 303 VTGPTVTPTYVNrpVATALakIDDNSEGFW--HRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVR 380
|
330 340 350
....*....|....*....|....*....|....*..
gi 734849183 544 EAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLELK 580
Cdd:cd05910 381 RSALVGVGKPGCQLPVLCVEPLPGTITPRARLEQELR 417
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
260-615 |
1.23e-09 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 60.88 E-value: 1.23e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 260 FILYTSGSTGKPKGVQHTTGGYL-LYAALThERVFDYRPGE--IYWCTADV----------GWVTGHTYIVYGPLANGAt 326
Cdd:cd17648 98 YAIYTSGTTGKPKGVLVEHGSVVnLRTSLS-ERYFGRDNGDeaVLFFSNYVfdffveqmtlALLNGQKLVVPPDEMRFD- 175
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 tllfegvpnyPDitRVAKIVDKHKVNILYTAPTAIRAMmasgtaacEGADGSSLRLLGSVGEPINPEAWDwyyKNVGQSR 406
Cdd:cd17648 176 ----------PD--RFYAYINREKVTYLSGTPSVLQQY--------DLARLPHLKRVDAAGEEFTAPVFE---KLRSRFA 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 407 CPIVDTWWQTETG-GNMMSPLPGAHALKpGSAARPFFGV-VPALVDNLGNLIEGpAEGNLVIldSWPGQARtlyGDH--- 481
Cdd:cd17648 233 GLIINAYGPTETTvTNHKRFFPGDQRFD-KSLGRPVRNTkCYVLNDAMKRVPVG-AVGELYL--GGDGVAR---GYLnrp 305
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 482 ----DRFVDTYFKT----FRG----MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:cd17648 306 eltaERFLPNPFQTeqerARGrnarLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVA 385
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 734849183 550 VPHDIKGQG-----IYVYVTLNGGEEPTEALRlelkNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17648 386 KEDASQAQSriqkyLVGYYLPEPGHVPESDLL----SFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
101-613 |
1.80e-09 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 60.78 E-value: 1.80e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 101 DPAESRTITYRELHEEVCKFANALRGQDVHRGDVVTIY--MP--MIPEAVVAMLACARIGAIHSvvfggfsPEAlagrii 176
Cdd:PRK07768 23 EPDAPVRHTWGEVHERARRIAGGLAAAGVGPGDAVAVLagAPveIAPTAQGLWMRGASLTMLHQ-------PTP------ 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 177 dcKSKVVITADEGIRAGKKIPLKANV-----DDALTNPETSSIQKVIVckrtngaikwnqhrdiwyEDLmkVAGAVCAPK 251
Cdd:PRK07768 90 --RTDLAVWAEDTLRVIGMIGAKAVVvgepfLAAAPVLEEKGIRVLTV------------------ADL--LAADPIDPV 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 252 EMGAEEALFILYTSGSTGKPKGVQHTTGGYLLYAALTHERVfDYRPGE---IYW--CTADVGWVTGHTYivygPLANGAT 326
Cdd:PRK07768 148 ETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAA-EFDVETdvmVSWlpLFHDMGMVGFLTV----PMYFGAE 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 TLLFEGVPNYPDITRVAKIVDKHKVNILyTAPTAIRAMMAS--GTAACEGA-DGSSLRLLGSVGEPINPEAWDWYY---K 400
Cdd:PRK07768 223 LVKVTPMDFLRDPLLWAELISKYRGTMT-AAPNFAYALLARrlRRQAKPGAfDLSSLRFALNGAEPIDPADVEDLLdagA 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 401 NVGQSRCPIVDTWWQTET---------GGNMMSPLPGAHALkpgsAARPFFgvVPALVDN------LGNLIEGpAEGNLV 465
Cdd:PRK07768 302 RFGLRPEAILPAYGMAEAtlavsfspcGAGLVVDEVDADLL----AALRRA--VPATKGNtrrlatLGPPLPG-LEVRVV 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 466 ILDSWPGQARTL-------------YGDHDRFVDTyfKTFRGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEI 532
Cdd:PRK07768 375 DEDGQVLPPRGVgvielrgesvtpgYLTMDGFIPA--QDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIYPTDI 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 533 ESAMVAHPKV--AEAAVVGVPHDIKGQGIYVYVTLNGGEEPTEALRLE--LKNWVRKEIGpiASP-DVIQWAPG-LPKTR 606
Cdd:PRK07768 453 ERAAARVEGVrpGNAVAVRLDAGHSREGFAVAVESNAFEDPAEVRRIRhqVAHEVVAEVG--VRPrNVVVLGPGsIPKTP 530
|
....*..
gi 734849183 607 SGKIMRR 613
Cdd:PRK07768 531 SGKLRRA 537
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
109-323 |
1.75e-08 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 57.52 E-value: 1.75e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 109 TYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALaGRIID-CKSKVVITAD 187
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEACAAHSLICVPLYDTLGPGAV-DYIVDhAEIDFVFVQD 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EGIRagkkiplkanvddALTNPETSSIQ--KVIVCKRTNGAIKWNQHRDI------WyEDLMKVA----GAVCAPKemgA 255
Cdd:PLN02430 157 KKIK-------------ELLEPDCKSAKrlKAIVSFTSVTEEESDKASQIgvktysW-IDFLHMGkenpSETNPPK---P 219
|
170 180 190 200 210 220
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 734849183 256 EEALFILYTSGSTGKPKGVqhttggyllyaALTHERVFDYRPG-EIYWCTADVGWVTGHTYIVYGPLAN 323
Cdd:PLN02430 220 LDICTIMYTSGTSGDPKGV-----------VLTHEAVATFVRGvDLFMEQFEDKMTHDDVYLSFLPLAH 277
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
106-551 |
4.71e-08 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 55.82 E-value: 4.71e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 106 RTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 adegiragkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdiwyedlmkvagavcapkemgaeEALFILYTS 265
Cdd:cd05940 82 -----------------------------------------------------------------------DAALYIYTS 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 266 GSTGKPKG--VQH------TTGGYLLYAALTHERVFDYRPgeIYWCTAD-VGWVTGhtyivygpLANGATTLL---FEGV 333
Cdd:cd05940 91 GTTGLPKAaiISHrrawrgGAFFAGSGGALPSDVLYTCLP--LYHSTALiVGWSAC--------LASGATLVIrkkFSAS 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 334 PNYPDITrvakivdKHKVNILYTAPTAIRAMMAsgTAACEGADGSSLRLLgsVGEPINPEAWDWYYKNVGQSRcpIVDTW 413
Cdd:cd05940 161 NFWDDIR-------KYQATIFQYIGELCRYLLN--QPPKPTERKHKVRMI--FGNGLRPDIWEEFKERFGVPR--IAEFY 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 414 WQTEtgGNM----MSPLPGAHALKPGSAARPF-FGVVPALVDNL-------GNLIE-GPAEGNLVILDSWPGQARTLYGD 480
Cdd:cd05940 228 AATE--GNSgfinFFGKPGAIGRNPSLLRKVApLALVKYDLESGepirdaeGRCIKvPRGEPGLLISRINPLEPFDGYTD 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 481 ---------HDRFV--DTYFKtfrgmyfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVG 549
Cdd:cd05940 306 paatekkilRDVFKkgDAWFN-------TGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYG 378
|
..
gi 734849183 550 VP 551
Cdd:cd05940 379 VQ 380
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
104-322 |
7.03e-08 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 55.75 E-value: 7.03e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:PTZ00216 118 ETRYITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAI 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITAdegiraGKKIPlkaNVDDALTNPETSSIqKVIVCKRTNGAIKWNQHRDIWYEDLMK-----VAGAVCAPKEMGAEEA 258
Cdd:PTZ00216 198 VCN------GKNVP---NLLRLMKSGGMPNT-TIIYLDSLPASVDTEGCRLVAWTDVVAkghsaGSHHPLNIPENNDDLA 267
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 259 LfILYTSGSTGKPKGVQHTTGGylLYAALT--HERVFD----YRPGEiywctadvgwvtghTYIVYGPLA 322
Cdd:PTZ00216 268 L-IMYTSGTTGDPKGVMHTHGS--LTAGILalEDRLNDligpPEEDE--------------TYCSYLPLA 320
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
107-277 |
1.25e-07 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 54.77 E-value: 1.25e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 107 TITYRELHEEVCKFANALR-GQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGrIIDCKSKVVIT 185
Cdd:cd17632 67 TITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAP-ILAETEPRLLA 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADegiragkkiplKANVDDALTN-PETSSIQKVIVckrTNGAIKWNQHRDIWYEDLMKVAGAVCA--------------- 249
Cdd:cd17632 146 VS-----------AEHLDLAVEAvLEGGTPPRLVV---FDHRPEVDAHRAALESARERLAAVGIPvttltliavrgrdlp 211
|
170 180 190
....*....|....*....|....*....|...
gi 734849183 250 -----PKEMGAEEALFILYTSGSTGKPKGVQHT 277
Cdd:cd17632 212 paplfRPEPDDDPLALLIYTSGSTGTPKGAMYT 244
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
498-617 |
7.29e-07 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 52.11 E-value: 7.29e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLNGG------EEP 571
Cdd:PLN02860 418 TGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQHPGVASVVVVGVPDSRLTEMVVACVRLRDGwiwsdnEKE 497
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*..
gi 734849183 572 TEALRLEL-----------KNWVRKEIGPIaspdVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PLN02860 498 NAKKNLTLssetlrhhcreKNLSRFKIPKL----FVQWRKPFPLTTTGKIRRDEVRR 550
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
532-648 |
8.10e-07 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 51.69 E-value: 8.10e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 532 IESAMVAHPKVAEAAVVGVPHDIKGQGIYVYV--TLNGGEEpteALRLELKnwvrkEIGPIASPDVIQWAPGLPKTRSGK 609
Cdd:PRK09188 245 IQAALKSDPAVSDVAIALFSLPAKGVGLYAFVeaELPADEK---SLRARLA-----GAKPPKPPEHIQPVAALPRDADGT 316
|
90 100 110 120
....*....|....*....|....*....|....*....|
gi 734849183 610 IMRRILRKIATGEYDGLGD-ISTLADPGVVAHLIETHKTM 648
Cdd:PRK09188 317 VRDDILRLIAMNQIDELDDlLREPEIRGLVEAIAAHRLNL 356
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
91-275 |
1.06e-06 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 51.82 E-value: 1.06e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 91 DQAAIIWEGDdpaesrTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIG------AIHSvvfg 164
Cdd:PRK04813 17 DFPAYDYLGE------KLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGhayipvDVSS---- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 165 gfsPEALAGRIID-CKSKVVITADEgiragkkiplkanvddalTNPETSSIQkvivckrtngAIKWNQHRDIWYEDlmkv 243
Cdd:PRK04813 87 ---PAERIEMIIEvAKPSLIIATEE------------------LPLEILGIP----------VITLDELKDIFATG---- 131
|
170 180 190
....*....|....*....|....*....|..
gi 734849183 244 aGAVCAPKEMGAEEALFILYTSGSTGKPKGVQ 275
Cdd:PRK04813 132 -NPYDFDHAVKGDDNYYIIFTSGTTGKPKGVQ 162
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
104-589 |
1.63e-06 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 51.06 E-value: 1.63e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd17639 2 EYKYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSAI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 184 ITAdegiraGKKiplkanvDDaltnpetssiqkvIVCkrtngaikwnqhrdiwyedlmkvagavcapkemgaeealfILY 263
Cdd:cd17639 82 FTD------GKP-------DD-------------LAC----------------------------------------IMY 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 264 TSGSTGKPKGVQHTTGGylLYAAlthervfdyrpgeiywCTADVGWVTGH-----TYIVYGPLAN-------------GA 325
Cdd:cd17639 96 TSGSTGNPKGVMLTHGN--LVAG----------------IAGLGDRVPELlgpddRYLAYLPLAHifelaaenvclyrGG 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 326 T-------TL------------------LFEGVPNYPDitRVAKIVDKhKVN--------ILYTAPTAIRAMMASG--TA 370
Cdd:cd17639 158 TigygsprTLtdkskrgckgdltefkptLMVGVPAIWD--TIRKGVLA-KLNpmgglkrtLFWTAYQSKLKALKEGpgTP 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 371 ACEG--------ADGSSLRLLGSVGEPINPEAWDWyYKNVGqsrCPIVDTWWQTETGGNMMSPLPGahALKPGSAARPFF 442
Cdd:cd17639 235 LLDElvfkkvraALGGRLRYMLSGGAPLSADTQEF-LNIVL---CPVIQGYGLTETCAGGTVQDPG--DLETGRVGPPLP 308
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 443 GVVPALVD--NLGN-----------LIEGPAegnlVILDSWPGQARTlygDHDRFVDTYFKtfrgmyfTGDGARRDEDGY 509
Cdd:cd17639 309 CCEIKLVDweEGGYstdkppprgeiLIRGPN----VFKGYYKNPEKT---KEAFDGDGWFH-------TGDIGEFHPDGT 374
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 510 YWITGRVDD-VLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVyvtlnggeePTEALrleLKNWVRKEIG 588
Cdd:cd17639 375 LKIIDRKKDlVKLQNGEYIALEKLESIYRSNPLVNNICVYADPDKSYPVAIVV---------PNEKH---LTKLAEKHGV 442
|
.
gi 734849183 589 P 589
Cdd:cd17639 443 I 443
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
108-277 |
5.13e-06 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 49.52 E-value: 5.13e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALR--GQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVIt 185
Cdd:cd05927 6 ISYKEVAERADNIGSALRslGGKPAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVF- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 186 ADEGIRagkkiplkanvddaltnpetssiqkvivckrtngaikwnqhrdIW-YEDLMKVAGAVCAPKEMGAEEALF-ILY 263
Cdd:cd05927 85 CDAGVK-------------------------------------------VYsLEEFEKLGKKNKVPPPPPKPEDLAtICY 121
|
170
....*....|....
gi 734849183 264 TSGSTGKPKGVQHT 277
Cdd:cd05927 122 TSGTTGNPKGVMLT 135
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
255-623 |
5.47e-06 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 49.41 E-value: 5.47e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 255 AEEALFILYTSGSTGKPKGV--QHTTGGYLLYAAL------THERVFDYRPgeiywCTADVGWVTGHtyivYGPLANGAT 326
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVmlTHENLVHNMFAILnstewkTKDRILSWMP-----LTHDMGLIAFH----LAPLIAGMN 175
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 327 TLLfegVPNYPDITRVA---KIVDKHKVNILYTAPTAIRAMMAS-GTAACEGADGSSLRLLGSVGEPINPEAWDWYYKNV 402
Cdd:cd05908 176 QYL---MPTRLFIRRPIlwlKKASEHKATIVSSPNFGYKYFLKTlKPEKANDWDLSSIRMILNGAEPIDYELCHEFLDHM 252
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 403 ---GQSRCPIVDTWWQTETGGNMMSPLPGAHALKPGSAARPFFGVVPA--------------------------LVDNLG 453
Cdd:cd05908 253 skyGLKRNAILPVYGLAEASVGASLPKAQSPFKTITLGRRHVTHGEPEpevdkkdsecltfvevgkpidetdirICDEDN 332
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 454 NLIEGPAEGNLVI--LDSWPGqartlYGDHDRFVDTYFkTFRGMYFTGD-GARRDEDGYywITGRVDDVLNVSGHRMGTA 530
Cdd:cd05908 333 KILPDGYIGHIQIrgKNVTPG-----YYNNPEATAKVF-TDDGWLKTGDlGFIRNGRLV--ITGREKDIIFVNGQNVYPH 404
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 531 EIESAMVAHPKV--AEAAVVGVpHD--IKGQGIYVYVTLNGGEEPTEALRLELKN-------WVRKEIGPIASpdviqwa 599
Cdd:cd05908 405 DIERIAEELEGVelGRVVACGV-NNsnTRNEEIFCFIEHRKSEDDFYPLGKKIKKhlnkrggWQINEVLPIRR------- 476
|
410 420
....*....|....*....|....*
gi 734849183 600 pgLPKTRSGKIMR-RILRKIATGEY 623
Cdd:cd05908 477 --IPKTTSGKVKRyELAQRYQSGEF 499
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
108-279 |
7.15e-06 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 49.07 E-value: 7.15e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 108 ITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAD 187
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVEFIINHAEVSIAFVQE 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 188 EGIRAGKKIPLK--ANVDDALTNPETSSIQKVIVCKRTNGAIKWNQHRDIW---YEDLMKVAGAVCApkemgaeealfIL 262
Cdd:PLN02861 158 SKISSILSCLPKcsSNLKTIVSFGDVSSEQKEEAEELGVSCFSWEEFSLMGsldCELPPKQKTDICT-----------IM 226
|
170
....*....|....*..
gi 734849183 263 YTSGSTGKPKGVQHTTG 279
Cdd:PLN02861 227 YTSGTTGEPKGVILTNR 243
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
486-612 |
1.55e-05 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 47.84 E-value: 1.55e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 486 DTYFKTFRGMYFTgdgarrdeDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKG--QGIYVYV 563
Cdd:PRK05851 395 DDWFPTGDLGYLV--------DGGLVVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSarPGLVIAA 466
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|
gi 734849183 564 TLNGGEEPTEalRLELKNWVRKEIGPIASpDVIQWAPG-LPKTRSGKIMR 612
Cdd:PRK05851 467 EFRGPDEAGA--RSEVVQRVASECGVVPS-DVVFVAPGsLPRTSSGKLRR 513
|
|
| hsFATP2a_ACSVL_like |
cd05938 |
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar ... |
104-292 |
1.12e-04 |
|
Fatty acid transport proteins (FATP) including hsFATP2, hsFATP5, and hsFATP6, and similar proteins; Fatty acid transport proteins (FATP) of this family transport long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes hsFATP2, hsFATP5, and hsFATP6, and similar proteins. Each FATP has unique patterns of tissue distribution. These FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. The hsFATP proteins exist in two splice variants; the b variant, lacking exon 3, has no acyl-CoA synthetase activity. FATPs are key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341261 [Multi-domain] Cd Length: 537 Bit Score: 45.36 E-value: 1.12e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 104 ESRTITYRELHEEVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACARIGAihSVVFGGFS--PEALAGRIIDCKS 180
Cdd:cd05938 2 EGETYTYRDVDRRSNQAARALLAHaGLRPGDTVALLLGNEPAFLWIWLGLAKLGC--PVAFLNTNirSKSLLHCFRCCGA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 181 KVVITADEgiragkkipLKANVDDALtnpetSSIQKVIVCkrtngaikwnqhrdIWY----------EDLM-KVAGAV-- 247
Cdd:cd05938 80 KVLVVAPE---------LQEAVEEVL-----PALRADGVS--------------VWYlshtsntegvISLLdKVDAASde 131
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|
gi 734849183 248 CAPKEMGAE-----EALFIlYTSGSTGKPKGvqhttggyllyAALTHERV 292
Cdd:cd05938 132 PVPASLRAHvtiksPALYI-YTSGTTGLPKA-----------ARISHLRV 169
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
103-274 |
4.03e-04 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 43.90 E-value: 4.03e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPE------------A 170
Cdd:TIGR03443 266 SKTRSFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPArqtiylsvakprA 345
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 171 L-----AGRIidcKSKVVITADEGIRAGKKIP-LKANVDDALtnpetssiqkvivckrTNGAIKwNQHRDIW--YEDLM- 241
Cdd:TIGR03443 346 LiviekAGTL---DQLVRDYIDKELELRTEIPaLALQDDGSL----------------VGGSLE-GGETDVLapYQALKd 405
|
170 180 190
....*....|....*....|....*....|...
gi 734849183 242 KVAGAVCAPKEMGAeealfILYTSGSTGKPKGV 274
Cdd:TIGR03443 406 TPTGVVVGPDSNPT-----LSFTSGSEGIPKGV 433
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
507-612 |
1.00e-03 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 42.30 E-value: 1.00e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 507 DGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKV--AEAAVVGVPHDiKGQGIYVYVTLNGG-EEPTEALRLELKNWV 583
Cdd:PRK09192 451 DGYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELrsGDAAAFSIAQE-NGEKIVLLVQCRISdEERRGQLIHALAALV 529
|
90 100 110
....*....|....*....|....*....|
gi 734849183 584 RKEIGpiASPDVIQWAP-GLPKTRSGKIMR 612
Cdd:PRK09192 530 RSEFG--VEAAVELVPPhSLPRTSSGKLSR 557
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
498-624 |
2.19e-03 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 41.31 E-value: 2.19e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 498 TGD-GARRDedGYYWITGRVDDVLNVSGHRMGTAEIESAM---VAHPKVAEAAVVGVPHD-IKGQGIYVYVTLNGGEE-P 571
Cdd:PRK05691 433 TGDlGFLRD--GELFVTGRLKDMLIVRGHNLYPQDIEKTVereVEVVRKGRVAAFAVNHQgEEGIGIAAEISRSVQKIlP 510
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*
gi 734849183 572 TEALRLELKNWVrKEIGPIASPDVIQWAPG-LPKTRSGKIMRRILR-KIATGEYD 624
Cdd:PRK05691 511 PQALIKSIRQAV-AEACQEAPSVVLLLNPGaLPKTSSGKLQRSACRlRLADGSLD 564
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
103-610 |
2.20e-03 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 40.96 E-value: 2.20e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 103 AESRTITYRELHEEVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPealagriidckskv 182
Cdd:cd17647 16 SKTRSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPP-------------- 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 183 vitadegirAGKKIPLKANVDDALTNpetssIQKvivckrtngaikwnqhrdiwyedlmkvAGAVCAPKEMGAeealfIL 262
Cdd:cd17647 82 ---------ARQNIYLGVAKPRGLIV-----IRA---------------------------AGVVVGPDSNPT-----LS 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 263 YTSGSTGKPKGV--QHTTGGYllYAALTHERvFDYRPGEIYwctadvgwvTGHTYIVYGPLANGATTLLFEG----VPNY 336
Cdd:cd17647 116 FTSGSEGIPKGVlgRHFSLAY--YFPWMAKR-FNLSENDKF---------TMLSGIAHDPIQRDMFTPLFLGaqllVPTQ 183
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 337 PDIT---RVAKIVDKHKVNILYTAPtairammASGTAACEGADGSSLRLLGS--VGEPINPEawDWYYKNVGQSRCPIVD 411
Cdd:cd17647 184 DDIGtpgRLAEWMAKYGATVTHLTP-------AMGQLLTAQATTPFPKLHHAffVGDILTKR--DCLRLQTLAENVRIVN 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 412 TWWQTETGGNMmsplpgAHALKPGSAARPFF-----GVVPA---------LVDN---------LGNLIE------GPAEG 462
Cdd:cd17647 255 MYGTTETQRAV------SYFEVPSRSSDPTFlknlkDVMPAgrgmlnvqlLVVNrndrtqicgIGEVGEiyvragGLAEG 328
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 463 NL--------VILDSW---PGQartlYGDHDRFVDTYFKTF-----RGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHR 526
Cdd:cd17647 329 YRglpelnkeKFVNNWfvePDH----WNYLDKDNNEPWRQFwlgprDRLYRTGDLGRYLPNGDCECCGRADDQVKIRGFR 404
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 527 MGTAEIESAMVAHPKVAE----------------AAVVGVPHDIKGQGIY-VYVTLNGGEEPT-------EALRLELKNW 582
Cdd:cd17647 405 IELGEIDTHISQHPLVREnitlvrrdkdeeptlvSYIVPRFDKPDDESFAqEDVPKEVSTDPIvkgligyRKLIKDIREF 484
|
570 580
....*....|....*....|....*...
gi 734849183 583 VRKEIGPIASPDVIQWAPGLPKTRSGKI 610
Cdd:cd17647 485 LKKRLASYAIPSLIVVLDKLPLNPNGKV 512
|
|
| PRK07868 |
PRK07868 |
acyl-CoA synthetase; Validated |
433-607 |
9.04e-03 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236121 [Multi-domain] Cd Length: 994 Bit Score: 39.32 E-value: 9.04e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 433 KPGSAARPFFG---VVPALVDNLGNLIEGPAEGNLVILDswPGQARTLYGDHDRFVDTYFKTFRGMYFTGDG-------A 502
Cdd:PRK07868 767 KIGSKGRPLPGagrVELAAYDPEHDLILEDDRGFVRRAE--VNEVGVLLARARGPIDPTASVKRGVFAPADTwisteylF 844
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 734849183 503 RRDEDGYYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVphDIKGQGIYVY-VTLNGGEEPTEAlrlELKN 581
Cdd:PRK07868 845 RRDDDGDYWLVDRRGSVIRTARGPVYTEPVTDALGRIGGVDLAVTYGV--EVGGRQLAVAaVTLRPGAAITAA---DLTE 919
|
170 180
....*....|....*....|....*.
gi 734849183 582 WVRkEIGPIASPDVIQWAPGLPKTRS 607
Cdd:PRK07868 920 ALA-SLPVGLGPDIVHVVPEIPLSAT 944
|
|
| |
