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Conserved domains on  [gi|746620436|ref|WP_039646208|]
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N-acetyltransferase [Staphylococcus hyicus]

Protein Classification

GNAT family protein( domain architecture ID 106742)

GNAT (Gcn5-related N-acetyltransferase) family protein similar to N-acetyltransferases that catalyze the transfer of an acetyl group from acetyl-CoA to a substrate

PubMed:  15581578

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NAT_SF super family cl17182
N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of ...
 1-148 8.10e-84

N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase which catalyze the transfer of an acetyl group to a substrate. The mechanism is an ordered Bi-Bi ternary complex kinetic mechanism for most GNATs: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and then CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/ph enylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


The actual alignment was detected with superfamily member PRK13688:

Pssm-ID: 473072  Cd Length: 156  Bit Score: 242.61  E-value: 8.10e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   1 MTKVVHLDINYRTEELFEEFRNFGNKDLYMVDELHGEMIDASSDSPFYGIYVGEKIGARMALYRKGEVEETYFPDYDDYL 80
Cdd:PRK13688   1 MPEVKRLLINYKTLEEFKKFREFGNQELSMLEELQANIIENDSESPFYGIYYGDSLVARMSLYKKGGVEEPYFEDTQDYL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 746620436  81 VVWKLEVLKKYQGKGYGTTLLEYAKSFNEPIKAIARFESKSFFIENGFKDLNAKNDDGHDILIWDPKH 148
Cdd:PRK13688  81 ELWKLEVLPKYQNRGYGEMLVDFAKSFQLPIKTIARNKSKDFWLKLGFTPVEYKNDEGEDPLVWHPET 148
 
Name Accession Description Interval E-value
PRK13688 PRK13688
N-acetyltransferase;
1-148 8.10e-84

N-acetyltransferase;


Pssm-ID: 237470  Cd Length: 156  Bit Score: 242.61  E-value: 8.10e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   1 MTKVVHLDINYRTEELFEEFRNFGNKDLYMVDELHGEMIDASSDSPFYGIYVGEKIGARMALYRKGEVEETYFPDYDDYL 80
Cdd:PRK13688   1 MPEVKRLLINYKTLEEFKKFREFGNQELSMLEELQANIIENDSESPFYGIYYGDSLVARMSLYKKGGVEEPYFEDTQDYL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 746620436  81 VVWKLEVLKKYQGKGYGTTLLEYAKSFNEPIKAIARFESKSFFIENGFKDLNAKNDDGHDILIWDPKH 148
Cdd:PRK13688  81 ELWKLEVLPKYQNRGYGEMLVDFAKSFQLPIKTIARNKSKDFWLKLGFTPVEYKNDEGEDPLVWHPET 148
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 74-129 6.95e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 37.28  E-value: 6.95e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 746620436  74 PDYDDYLVVWKLEVLKKYQGKGYGTTLLEY----AKSFN-EPIKAIARFESKSFFIENGFK 129
Cdd:COG1246   47 PLDEDLAELRSLAVHPDYRGRGIGRRLLEAllaeARELGlKRLFLLTTSAAIHFYEKLGFE 107
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 47-107 7.92e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.10  E-value: 7.92e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 746620436  47 FYGIYVGEKIGARMALYRkgeveetyFPDYDDYLVVWKLEVLKKYQGKGYGTTLLEYAKSF 107
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP--------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 13-131 3.38e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 35.32  E-value: 3.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   13 TEELFEEFRNFgnkdlyMVDELHGEMIDaSSDSPFYGIYVGEKIGARMALYRKGEVeetyfpdyddYLVVwkleVLKKYQ 92
Cdd:pfam13673   6 SEEGIETFYEF------ISPEALRERID-QGEYFFFVAFEGGQIVGVIALRDRGHI----------SLLF----VDPDYQ 64

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 746620436   93 GKGYGTTLLEYAKSFNEPIK-AIARFE------SKSFFIENGFKDL 131
Cdd:pfam13673  65 GQGIGKALLEAVEDYAEKDGiKLSELTvnaspyAVPFYEKLGFRAT 110
 
Name Accession Description Interval E-value
PRK13688 PRK13688
N-acetyltransferase;
1-148 8.10e-84

N-acetyltransferase;


Pssm-ID: 237470  Cd Length: 156  Bit Score: 242.61  E-value: 8.10e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   1 MTKVVHLDINYRTEELFEEFRNFGNKDLYMVDELHGEMIDASSDSPFYGIYVGEKIGARMALYRKGEVEETYFPDYDDYL 80
Cdd:PRK13688   1 MPEVKRLLINYKTLEEFKKFREFGNQELSMLEELQANIIENDSESPFYGIYYGDSLVARMSLYKKGGVEEPYFEDTQDYL 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 746620436  81 VVWKLEVLKKYQGKGYGTTLLEYAKSFNEPIKAIARFESKSFFIENGFKDLNAKNDDGHDILIWDPKH 148
Cdd:PRK13688  81 ELWKLEVLPKYQNRGYGEMLVDFAKSFQLPIKTIARNKSKDFWLKLGFTPVEYKNDEGEDPLVWHPET 148
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 74-129 6.95e-04

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 37.28  E-value: 6.95e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 746620436  74 PDYDDYLVVWKLEVLKKYQGKGYGTTLLEY----AKSFN-EPIKAIARFESKSFFIENGFK 129
Cdd:COG1246   47 PLDEDLAELRSLAVHPDYRGRGIGRRLLEAllaeARELGlKRLFLLTTSAAIHFYEKLGFE 107
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 47-107 7.92e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.10  E-value: 7.92e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 746620436  47 FYGIYVGEKIGARMALYRkgeveetyFPDYDDYLVVWKLEVLKKYQGKGYGTTLLEYAKSF 107
Cdd:cd04301    1 FLVAEDDGEIVGFASLSP--------DGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEE 53
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 13-131 3.38e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 35.32  E-value: 3.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   13 TEELFEEFRNFgnkdlyMVDELHGEMIDaSSDSPFYGIYVGEKIGARMALYRKGEVeetyfpdyddYLVVwkleVLKKYQ 92
Cdd:pfam13673   6 SEEGIETFYEF------ISPEALRERID-QGEYFFFVAFEGGQIVGVIALRDRGHI----------SLLF----VDPDYQ 64

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 746620436   93 GKGYGTTLLEYAKSFNEPIK-AIARFE------SKSFFIENGFKDL 131
Cdd:pfam13673  65 GQGIGKALLEAVEDYAEKDGiKLSELTvnaspyAVPFYEKLGFRAT 110
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 72-104 4.92e-03

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 34.25  E-value: 4.92e-03
                         10        20        30
                 ....*....|....*....|....*....|...
gi 746620436  72 YFPDYDDYLVVWKLEVLKKYQGKGYGTTLLEYA 104
Cdd:COG0456    6 GLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAA 38
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 14-104 4.97e-03

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 34.80  E-value: 4.97e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 746620436   14 EELFEEFRNFGNKDLYMVDELHGEMIDASSDSPFYGIYVGEKIgARMALYRKgeveetyFPDYDDYLVVWKLEVLKKYQG 93
Cdd:pfam00583   2 EALYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGEL-VGFASLSI-------IDDEPPVGEIEGLAVAPEYRG 73

                          90
                  ....*....|.
gi 746620436   94 KGYGTTLLEYA 104
Cdd:pfam00583  74 KGIGTALLQAL 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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