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Conserved domains on  [gi|1332876552|ref|WP_102838829|]
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MULTISPECIES: sterol desaturase family protein [Stutzerimonas stutzeri group]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase super family cl01132
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 16-141 9.47e-28

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


The actual alignment was detected with superfamily member PLN02601:

Pssm-ID: 412761  Cd Length: 303  Bit Score: 104.77  E-value: 9.47e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552  16 GMEVFAWWAHKYVMHGWGWGWHKSHHEPRNGWFEKNDLYAVVFAGVAILLIALG--TAGYHPLEWIGAGM--TAYGFLYF 91
Cdd:PLN02601  146 GMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAIVNAVPAIGLLYYGffNKGLVPGLCFGAGLgiTVFGMAYM 225

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1332876552  92 VAHDGLVHHRWPLRYVPRNGYLKRLYQAHRLHHAveGKERCVSFGFLYAP 141
Cdd:PLN02601  226 FVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHT--DKFKGVPYGLFLGP 273
 
Name Accession Description Interval E-value
PLN02601 PLN02601
beta-carotene hydroxylase
 16-141 9.47e-28

beta-carotene hydroxylase


Pssm-ID: 178211  Cd Length: 303  Bit Score: 104.77  E-value: 9.47e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552  16 GMEVFAWWAHKYVMHGWGWGWHKSHHEPRNGWFEKNDLYAVVFAGVAILLIALG--TAGYHPLEWIGAGM--TAYGFLYF 91
Cdd:PLN02601  146 GMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAIVNAVPAIGLLYYGffNKGLVPGLCFGAGLgiTVFGMAYM 225

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1332876552  92 VAHDGLVHHRWPLRYVPRNGYLKRLYQAHRLHHAveGKERCVSFGFLYAP 141
Cdd:PLN02601  226 FVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHT--DKFKGVPYGLFLGP 273
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 10-125 4.02e-05

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 41.26  E-value: 4.02e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552  10 FLATLAGMEVFAWWAHKyVMHGWGWGW---HKSHHEPRNGWFEKNDLYAVVFAGVAILLIALGTA--GYHPLEWIgAGMT 84
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWLWrrfHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLllGLPLLAFL-LGLL 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1332876552  85 AYGFLYFVAHDGlvhhrWPLRYVPRNGYLKRLYQAHRLHHA 125
Cdd:pfam04116  79 LGTLWYLFIHSG-----LLFPLPWLLKRLLGTPRFHRLHHS 114
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 9-130 7.42e-03

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 34.91  E-value: 7.42e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552   9 VFLATLAGMEVFAWWAHkYVMHGWGWGW--HKSHHEPRNGW----FEKNDLYAVVFAGVAILLIALgtAGYHPlEWIGAG 82
Cdd:COG3000     1 QFLLAFLLLDFVYYWLH-RLSHRVPLLWrfHAVHHSSERMNlltaLRFHPLEILLSALLFLLPLAL--LGFPP-EAVLLY 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1332876552  83 MTAYGFLYFVAHDGLVHH-RWPLRYV---PRNgylkrlyqaHRLHHAVEGKE 130
Cdd:COG3000    77 LALNLLYQFFNHSNIRLPlDGPLEYVfvtPSH---------HRVHHSRNPEY 119
 
Name Accession Description Interval E-value
PLN02601 PLN02601
beta-carotene hydroxylase
 16-141 9.47e-28

beta-carotene hydroxylase


Pssm-ID: 178211  Cd Length: 303  Bit Score: 104.77  E-value: 9.47e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552  16 GMEVFAWWAHKYVMHGWGWGWHKSHHEPRNGWFEKNDLYAVVFAGVAILLIALG--TAGYHPLEWIGAGM--TAYGFLYF 91
Cdd:PLN02601  146 GMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAIVNAVPAIGLLYYGffNKGLVPGLCFGAGLgiTVFGMAYM 225

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1332876552  92 VAHDGLVHHRWPLRYVPRNGYLKRLYQAHRLHHAveGKERCVSFGFLYAP 141
Cdd:PLN02601  226 FVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHT--DKFKGVPYGLFLGP 273
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 10-125 4.02e-05

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 41.26  E-value: 4.02e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552  10 FLATLAGMEVFAWWAHKyVMHGWGWGW---HKSHHEPRNGWFEKNDLYAVVFAGVAILLIALGTA--GYHPLEWIgAGMT 84
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWLWrrfHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLllGLPLLAFL-LGLL 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1332876552  85 AYGFLYFVAHDGlvhhrWPLRYVPRNGYLKRLYQAHRLHHA 125
Cdd:pfam04116  79 LGTLWYLFIHSG-----LLFPLPWLLKRLLGTPRFHRLHHS 114
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 9-130 7.42e-03

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 34.91  E-value: 7.42e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1332876552   9 VFLATLAGMEVFAWWAHkYVMHGWGWGW--HKSHHEPRNGW----FEKNDLYAVVFAGVAILLIALgtAGYHPlEWIGAG 82
Cdd:COG3000     1 QFLLAFLLLDFVYYWLH-RLSHRVPLLWrfHAVHHSSERMNlltaLRFHPLEILLSALLFLLPLAL--LGFPP-EAVLLY 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1332876552  83 MTAYGFLYFVAHDGLVHH-RWPLRYV---PRNgylkrlyqaHRLHHAVEGKE 130
Cdd:COG3000    77 LALNLLYQFFNHSNIRLPlDGPLEYVfvtPSH---------HRVHHSRNPEY 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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