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Conserved domains on  [gi|1865766052|ref|WP_177451831|]
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peptide chain release factor 2 [Clostridioides difficile]

Protein Classification

PCRF domain-containing protein( domain architecture ID 1750053)

peptide chain release factor (PCRF) domain-containing protein similar to Homo sapiens mitochondrial peptide chain release factor 1 (MTRF1), MTRF1-like and 28S mitochondrial ribosomal protein S35 (MRPS35)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PCRF super family cl40670
This domain is found in peptide chain release factors;
4-363 1.47e-174

This domain is found in peptide chain release factors;


The actual alignment was detected with superfamily member TIGR00020:

Pssm-ID: 477417 [Multi-domain]  Cd Length: 364  Bit Score: 489.67  E-value: 1.47e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052   4 LQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVL 83
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  84 IEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKV 163
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 164 KLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDT 243
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDDDIDIEIKPEDLRIDT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 244 YRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQI 323
Cdd:TIGR00020 242 YRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQI 321

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 1865766052 324 RSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLK 363
Cdd:TIGR00020 322 RSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLK 361
 
Name Accession Description Interval E-value
prfB TIGR00020
peptide chain release factor 2; In many but not all taxa, there is a conserved real ...
 4-363 1.47e-174

peptide chain release factor 2; In many but not all taxa, there is a conserved real translational frameshift at a TGA codon. RF-2 helps terminate translation at TGA codons and can therefore regulate its own production by readthrough when RF-2 is insufficient. There is a Pfam model called "RF-1" for the superfamily of RF-1, RF-2, mitochondrial, RF-H, etc. [Protein synthesis, Translation factors]


Pssm-ID: 272858 [Multi-domain]  Cd Length: 364  Bit Score: 489.67  E-value: 1.47e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052   4 LQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVL 83
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  84 IEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKV 163
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 164 KLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDT 243
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDDDIDIEIKPEDLRIDT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 244 YRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQI 323
Cdd:TIGR00020 242 YRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQI 321

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 1865766052 324 RSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLK 363
Cdd:TIGR00020 322 RSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLK 361
PRK05589 PRK05589
peptide chain release factor 2; Provisional
 41-366 7.56e-168

peptide chain release factor 2; Provisional


Pssm-ID: 235520 [Multi-domain]  Cd Length: 325  Bit Score: 471.21  E-value: 7.56e-168
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  41 MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLN 120
Cdd:PRK05589    1 MQEPNFWNDIKEAQEITSEEKYLKDKLDKYNHLRNRIEDIEVLCEM-MSEEDDEMKKEIISEVKNIKEEIDRFKIETLLS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 121 GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHR 200
Cdd:PRK05589   80 GEYDRNNAILTLHSGVGGTDAQDWTEMLLRMYTRWAEKKGYKVEIIDLLEGDEAGIKSVTLKITGEFAYGYLKAEKGIHR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 201 LVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ 280
Cdd:PRK05589  160 LVRISPFNANGKRQTSFASVEVLPELTDDQDIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQ 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 281 HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINE 360
Cdd:PRK05589  240 HSNKETAMKMLKSKLVELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGVETSNVDSVMDGDIDNFITQ 319


                  ....*.
gi 1865766052 361 YLKMNK 366
Cdd:PRK05589  320 YLKGNF 325
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 38-359 1.28e-99

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 298.84  E-value: 1.28e-99
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  38 EEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDDSVERDIEKS-IESMEEKLSEM--K 114
Cdd:COG0216    17 EALLSDPEVISDQKRFRKLSKEYAELEPIVEAYREYKKLLEDIEEAKEL-LEEESDPEMREMAKEeLEELEARLEELeeE 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 115 IKTLL-----NgeyDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIiSDPEA-GIKTATILVEGTNA 188
Cdd:COG0216    96 LKILLlpkdpN---DDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERKGWKVEILSA-SEGELgGYKEVIFRISGEGV 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 189 YGYLKSEKGVHRLVRIspfdP----SGKRHTSFASIDVTPELDEnIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRIT 264
Cdd:COG0216   172 YSRLKFESGVHRVQRV----PetesQGRIHTSAATVAVLPEAEE-VDVEINPKDLRIDTFRSSGAGGQHVNTTDSAVRIT 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 265 HIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDiQGKySQItwGS-----QIRSYVFqPYKLVKDHRT 339
Cdd:COG0216   247 HLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAA-ERK-SQV--GSgdrseRIRTYNF-PQGRVTDHRI 321

                         330       340
                  ....*....|....*....|
gi 1865766052 340 NAEFGNVDSVMNGNIDLFIN 359
Cdd:COG0216   322 NLTLYKLDEVMEGDLDELID 341
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 38-219 2.37e-72

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 223.41  E-value: 2.37e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  38 EEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDdsvERDIEKS-IESMEEKLSEM--K 114
Cdd:pfam03462   9 EALLSDPDVWDDQKRAQKLSKEYSELEPIVEAYREYKQALEDLEEAKEL-LEDPE---LAELAEEeLEELEKRLEELeeE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 115 IKTLLN--GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDiISDPEA-GIKTATILVEGTNAYGY 191
Cdd:pfam03462  85 LKLLLLpkDPNDDKNAILEIRAGAGGDEAALFAGDLFRMYTRYAERKGWKVEVLD-YSETEAgGIKEATFEISGEGAYGR 163

                         170       180
                  ....*....|....*....|....*...
gi 1865766052 192 LKSEKGVHRLVRISPFDPSGKRHTSFAS 219
Cdd:pfam03462 164 LKFESGVHRVQRVPPTESQGRIHTSFAT 191
PCRF smart00937
This domain is found in peptide chain release factors;
85-195 1.06e-39

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 136.82  E-value: 1.06e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052   85 EIGLEEDDDSVERDIEKSIESME----EKLSEMKIKTLLNG-EYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESK 159
Cdd:smart00937   1 ELLEEEEDDEMRELAEEELEELEaeleEELEELLKLLLLPKdPNDDKNAILEIRAGAGGDEAALFAGDLLRMYLRYAERK 80

                           90       100       110
                   ....*....|....*....|....*....|....*.
gi 1865766052  160 GYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSE 195
Cdd:smart00937  81 GWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
 
Name Accession Description Interval E-value
prfB TIGR00020
peptide chain release factor 2; In many but not all taxa, there is a conserved real ...
 4-363 1.47e-174

peptide chain release factor 2; In many but not all taxa, there is a conserved real translational frameshift at a TGA codon. RF-2 helps terminate translation at TGA codons and can therefore regulate its own production by readthrough when RF-2 is insufficient. There is a Pfam model called "RF-1" for the superfamily of RF-1, RF-2, mitochondrial, RF-H, etc. [Protein synthesis, Translation factors]


Pssm-ID: 272858 [Multi-domain]  Cd Length: 364  Bit Score: 489.67  E-value: 1.47e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052   4 LQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVL 83
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  84 IEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKV 163
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 164 KLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDT 243
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDDDIDIEIKPEDLRIDT 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 244 YRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQI 323
Cdd:TIGR00020 242 YRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQI 321

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|
gi 1865766052 324 RSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLK 363
Cdd:TIGR00020 322 RSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLK 361
PRK05589 PRK05589
peptide chain release factor 2; Provisional
 41-366 7.56e-168

peptide chain release factor 2; Provisional


Pssm-ID: 235520 [Multi-domain]  Cd Length: 325  Bit Score: 471.21  E-value: 7.56e-168
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  41 MNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLN 120
Cdd:PRK05589    1 MQEPNFWNDIKEAQEITSEEKYLKDKLDKYNHLRNRIEDIEVLCEM-MSEEDDEMKKEIISEVKNIKEEIDRFKIETLLS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 121 GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHR 200
Cdd:PRK05589   80 GEYDRNNAILTLHSGVGGTDAQDWTEMLLRMYTRWAEKKGYKVEIIDLLEGDEAGIKSVTLKITGEFAYGYLKAEKGIHR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 201 LVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQ 280
Cdd:PRK05589  160 LVRISPFNANGKRQTSFASVEVLPELTDDQDIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQ 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 281 HLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINE 360
Cdd:PRK05589  240 HSNKETAMKMLKSKLVELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGVETSNVDSVMDGDIDNFITQ 319


                  ....*.
gi 1865766052 361 YLKMNK 366
Cdd:PRK05589  320 YLKGNF 325
PRK07342 PRK07342
peptide chain release factor 2; Provisional
 39-362 2.02e-131

peptide chain release factor 2; Provisional


Pssm-ID: 235997 [Multi-domain]  Cd Length: 339  Bit Score: 379.61  E-value: 2.02e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  39 EKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTL 118
Cdd:PRK07342    1 NKAEDPSLWNDAQEAQKLMRERQQLDDSINGINHLEQTLNDNIELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDAL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 119 LNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGV 198
Cdd:PRK07342   81 LSGEADANDTYLEVHAGAGGTESQDWASMLLRMYTRWAERQGRKVEVLEVHDGEEAGIKSATILVKGHNAYGWLKTESGV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 199 HRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNER 278
Cdd:PRK07342  161 HRLVRISPYDSNARRHTSFASIWVYPVIDDNIEVDVNESDVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVQCQQER 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 279 SQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFI 358
Cdd:PRK07342  241 SQHKNRAKAWSMLRARLYEEELKKREEATNAAAASKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQDVLDGDLNEFM 320


                  ....
gi 1865766052 359 NEYL 362
Cdd:PRK07342  321 EAAL 324
PRK06746 PRK06746
peptide chain release factor 2; Provisional
 46-363 2.15e-124

peptide chain release factor 2; Provisional


Pssm-ID: 75726 [Multi-domain]  Cd Length: 326  Bit Score: 361.36  E-value: 2.15e-124
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  46 FWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDK 125
Cdd:PRK06746    6 FWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVKGLIQEMNEYELQLLLSDPYDK 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 126 NNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRIS 205
Cdd:PRK06746   86 NNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRIS 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 206 PFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKD 285
Cdd:PRK06746  166 PFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQIKNRE 245

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1865766052 286 RAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLK 363
Cdd:PRK06746  246 HAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDAYLR 323
PRK08787 PRK08787
peptide chain release factor 2; Provisional
 62-363 1.48e-109

peptide chain release factor 2; Provisional


Pssm-ID: 136970 [Multi-domain]  Cd Length: 313  Bit Score: 323.15  E-value: 1.48e-109
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  62 SLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDA 141
Cdd:PRK08787    1 MLEKTVIGIADVLSGLADAGELLDLAESEQDEDTALAVIADLDKYQAHVEKLEFQRMFSGQMDGANAFVDIQAGAGGTEA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 142 QDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFASID 221
Cdd:PRK08787   81 QDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFTSVF 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 222 VTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKEL 301
Cdd:PRK08787  161 VSPEVDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRDNAMKMLAAKLYELEVQ 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1865766052 302 EQKEKIEDIQGKYSQITWGSQIRSYVFQPYKlVKDHRTNAEFGNVDSVMNGNIDLFINEYLK 363
Cdd:PRK08787  241 KRNAEKDALEATKSDIGWGSQIRNYVLDQSR-IKDLRTGIERSDTQKVLDGDLDEFVEASLK 301
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 38-359 1.28e-99

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 298.84  E-value: 1.28e-99
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  38 EEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDDSVERDIEKS-IESMEEKLSEM--K 114
Cdd:COG0216    17 EALLSDPEVISDQKRFRKLSKEYAELEPIVEAYREYKKLLEDIEEAKEL-LEEESDPEMREMAKEeLEELEARLEELeeE 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 115 IKTLL-----NgeyDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIiSDPEA-GIKTATILVEGTNA 188
Cdd:COG0216    96 LKILLlpkdpN---DDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERKGWKVEILSA-SEGELgGYKEVIFRISGEGV 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 189 YGYLKSEKGVHRLVRIspfdP----SGKRHTSFASIDVTPELDEnIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRIT 264
Cdd:COG0216   172 YSRLKFESGVHRVQRV----PetesQGRIHTSAATVAVLPEAEE-VDVEINPKDLRIDTFRSSGAGGQHVNTTDSAVRIT 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 265 HIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDiQGKySQItwGS-----QIRSYVFqPYKLVKDHRT 339
Cdd:COG0216   247 HLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAA-ERK-SQV--GSgdrseRIRTYNF-PQGRVTDHRI 321

                         330       340
                  ....*....|....*....|
gi 1865766052 340 NAEFGNVDSVMNGNIDLFIN 359
Cdd:COG0216   322 NLTLYKLDEVMEGDLDELID 341
prfA PRK00591
peptide chain release factor 1; Validated
 38-359 4.65e-98

peptide chain release factor 1; Validated


Pssm-ID: 234801 [Multi-domain]  Cd Length: 359  Bit Score: 295.06  E-value: 4.65e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  38 EEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEM--KI 115
Cdd:PRK00591   19 EALLSDPEVISDQKRFRKLSKEYAELEPIVEAYREYKQAQEDLEEAKEMLEEESDPEMREMAKEELKELEERLEELeeEL 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 116 KTLL-----NgeyDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIiSDPEA-GIKTATILVEGTNAY 189
Cdd:PRK00591   99 KILLlpkdpN---DDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERQGWKVEILSA-SEGELgGYKEVIAEISGDGVY 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 190 GYLKSEKGVHRLVRIsPFDPSGKR-HTSFASIDVTPELDEnIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPT 268
Cdd:PRK00591  175 SKLKFESGVHRVQRV-PATESQGRiHTSAATVAVLPEAEE-VEVEINPKDLRIDTFRSSGAGGQHVNTTDSAVRITHLPT 252

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 269 GVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDiqGKYSQItwGS-----QIRSYVFqPYKLVKDHRTNAEF 343
Cdd:PRK00591  253 GIVVECQDERSQHKNKAKAMKVLRARLYDAERQKAQAEEAA--TRKSQV--GSgdrseRIRTYNF-PQGRVTDHRINLTL 327

                         330
                  ....*....|....*.
gi 1865766052 344 GNVDSVMNGNIDLFIN 359
Cdd:PRK00591  328 YKLDEVMEGDLDELID 343
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 38-219 2.37e-72

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 223.41  E-value: 2.37e-72
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052  38 EEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIgLEEDDdsvERDIEKS-IESMEEKLSEM--K 114
Cdd:pfam03462   9 EALLSDPDVWDDQKRAQKLSKEYSELEPIVEAYREYKQALEDLEEAKEL-LEDPE---LAELAEEeLEELEKRLEELeeE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 115 IKTLLN--GEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDiISDPEA-GIKTATILVEGTNAYGY 191
Cdd:pfam03462  85 LKLLLLpkDPNDDKNAILEIRAGAGGDEAALFAGDLFRMYTRYAERKGWKVEVLD-YSETEAgGIKEATFEISGEGAYGR 163

                         170       180
                  ....*....|....*....|....*...
gi 1865766052 192 LKSEKGVHRLVRISPFDPSGKRHTSFAS 219
Cdd:pfam03462 164 LKFESGVHRVQRVPPTESQGRIHTSFAT 191
RF-1 pfam00472
RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and ...
 228-338 4.26e-66

RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and a number of smaller proteins of unknown function. This domain contains the peptidyl-tRNA hydrolase activity. The domain contains a highly conserved motif GGQ, where the glutamine is thought to coordinate the water that mediates the hydrolysis.


Pssm-ID: 459823 [Multi-domain]  Cd Length: 111  Bit Score: 204.58  E-value: 4.26e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 228 ENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEKI 307
Cdd:pfam00472   1 DEIEIEIDPKDLRIDTFRSSGAGGQHVNKTDSAVRLTHIPTGIVVKCQDERSQHQNREKALKRLRARLYELEEEKEEEKK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1865766052 308 EDIQGKYSQITWGSQIRSYVFQPYKLVKDHR 338
Cdd:pfam00472  81 RRIKSQVGSGDRSEQIRTYNFKPQSRVKDHR 111
PrfB COG1186
Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein ...
 231-345 2.00e-47

Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein chain release factor PrfB is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440799 [Multi-domain]  Cd Length: 140  Bit Score: 157.60  E-value: 2.00e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 231 EVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITH------------IPTGVVVQCQNERSQHLNKDRAMRLLMAKLIEL 298
Cdd:COG1186    20 EIEIPESDLRIDTYRASGAGGQNVNKVSSAVRLTHdiassslpdsrlTPGGIVVKAQEERSQHQNREDALKRLKALLYEA 99

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1865766052 299 KELEQKEK-IEDIQGKYSQITWGSQIRSyvfqpykLVKDHRTNAEFGN 345
Cdd:COG1186   100 ELEPKERRaTKPTRGSKKRRLWGKQIRS-------MVKDLRGNVDDGD 140
PCRF smart00937
This domain is found in peptide chain release factors;
85-195 1.06e-39

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 136.82  E-value: 1.06e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052   85 EIGLEEDDDSVERDIEKSIESME----EKLSEMKIKTLLNG-EYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESK 159
Cdd:smart00937   1 ELLEEEEDDEMRELAEEELEELEaeleEELEELLKLLLLPKdPNDDKNAILEIRAGAGGDEAALFAGDLLRMYLRYAERK 80

                           90       100       110
                   ....*....|....*....|....*....|....*.
gi 1865766052  160 GYKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSE 195
Cdd:smart00937  81 GWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
release_prfH TIGR03072
putative peptide chain release factor H; Members of this protein family are bacterial proteins ...
 130-305 2.35e-29

putative peptide chain release factor H; Members of this protein family are bacterial proteins homologous to peptide chain release factors 1 (RF-1, product of the prfA gene), and 2 (RF-2, product of the prfB gene). The member from Escherichia coli K-12, designated prfH, appears to be a pseudogene. This class I release factor is always found as the downstream gene of a two-gene operon. [Protein synthesis, Translation factors]


Pssm-ID: 213768  Cd Length: 200  Bit Score: 112.17  E-value: 2.35e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 130 LSINAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAG-IKTATILVEGTNAYGYLKSEKGVHRLVRISPFD 208
Cdd:TIGR03072   3 LQLSSAQGPAECCLAVAKALERLTREAAARGVRVEVLEQEPGEVPGtLRSALVSLDGEAAAALADRWEGTLLWICPSPYR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 209 PSGKRHTSFASIDVTPELDEnieveINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAM 288
Cdd:TIGR03072  83 PHHRRKNWFIGVQRFSASEE-----ATEDEIRFETLRSSGPGGQHVNKTESAVRATHLASGISVKVQSERSQHANKRLAT 157

                         170
                  ....*....|....*..
gi 1865766052 289 RLLMAKLIELKELEQKE 305
Cdd:TIGR03072 158 LLLAVRLADLQQEQAAA 174
PRK09256 PRK09256
aminoacyl-tRNA hydrolase;
232-298 1.73e-09

aminoacyl-tRNA hydrolase;


Pssm-ID: 181730 [Multi-domain]  Cd Length: 138  Bit Score: 55.54  E-value: 1.73e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1865766052 232 VEINPSDLKIDTYRASGAGGQHVNTTDSAV-------------------------RIThiPTGV-VVQCQNERSQHLNKD 285
Cdd:PRK09256    8 LVIPENELEWRFIRASGPGGQNVNKVSTAVelrfdiaasslpefykerllalaghRIT--KDGViVIKAQEFRSQERNRE 85

                          90
                  ....*....|...
gi 1865766052 286 RAmrllMAKLIEL 298
Cdd:PRK09256   86 DA----LERLVAL 94
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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