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Conserved domains on  [gi|124805449|ref|XP_001350443|]
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major facilitator superfamily domain-containing protein, putative [Plasmodium falciparum 3D7]

Protein Classification

MFS transporter( domain architecture ID 10150691)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085|GO:0016020
PubMed:  26758938|26098515|26234418
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 1019-1191 1.25e-09

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


:

Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 61.67  E-value: 1.25e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1019 IISLCILNFLFNFLYISFFIIYNIFIYDIkssqnFYKMNDSFIILNSIILIFYIFLYFYLHRIILKILNLFGYLLITFVS 1098
Cdd:cd06174   205 LWLLLLAIFLVNLAYYSFSTLLPLFLLDL-----GGLSVAVAGLLLSLFGLAGALGSLLLGLLSDRLIGRKPLLLIGLLL 279

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1099 FSLILFLYSTSYFHISLydntyIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFVSYTLFRFIIQE 1178
Cdd:cd06174   280 MALGLALLLLAPSLLLL-----LLLLLLLGFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLLA 354

                         170
                  ....*....|...
gi 124805449 1179 QNMTLYNLIFSCV 1191
Cdd:cd06174   355 ATFGLTGAFLVLA 367
 
Name Accession Description Interval E-value
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 1019-1191 1.25e-09

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 61.67  E-value: 1.25e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1019 IISLCILNFLFNFLYISFFIIYNIFIYDIkssqnFYKMNDSFIILNSIILIFYIFLYFYLHRIILKILNLFGYLLITFVS 1098
Cdd:cd06174   205 LWLLLLAIFLVNLAYYSFSTLLPLFLLDL-----GGLSVAVAGLLLSLFGLAGALGSLLLGLLSDRLIGRKPLLLIGLLL 279

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1099 FSLILFLYSTSYFHISLydntyIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFVSYTLFRFIIQE 1178
Cdd:cd06174   280 MALGLALLLLAPSLLLL-----LLLLLLLGFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLLA 354

                         170
                  ....*....|...
gi 124805449 1179 QNMTLYNLIFSCV 1191
Cdd:cd06174   355 ATFGLTGAFLVLA 367
MFS_1 pfam07690
Major Facilitator Superfamily;
1018-1167 3.93e-04

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 44.33  E-value: 3.93e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449  1018 LIISLCILNFLFNFLYISFFIIYnIFIYDIKSSQNFYkmndSFIILNSIILIFYIFLYFYLHRIILKILNLFGYLLITFV 1097
Cdd:pfam07690  204 WLLLALLLFGFAFFGLLTYLPLY-QEVLGLSALLAGL----LLGLGGLLGAIGRLLLGRLSDRLGRRRRLLLALLLLILA 278

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449  1098 SFSLILFLYSTSYFhislydnTYIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFV 1167
Cdd:pfam07690  279 ALGLLLLSLTLSSL-------WLLLALLLLGFGFGLVFPALNALVSDLAPKEERGTASGLYNTAGSLGGA 341
 
Name Accession Description Interval E-value
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 1019-1191 1.25e-09

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 61.67  E-value: 1.25e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1019 IISLCILNFLFNFLYISFFIIYNIFIYDIkssqnFYKMNDSFIILNSIILIFYIFLYFYLHRIILKILNLFGYLLITFVS 1098
Cdd:cd06174   205 LWLLLLAIFLVNLAYYSFSTLLPLFLLDL-----GGLSVAVAGLLLSLFGLAGALGSLLLGLLSDRLIGRKPLLLIGLLL 279

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1099 FSLILFLYSTSYFHISLydntyIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFVSYTLFRFIIQE 1178
Cdd:cd06174   280 MALGLALLLLAPSLLLL-----LLLLLLLGFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLLA 354

                         170
                  ....*....|...
gi 124805449 1179 QNMTLYNLIFSCV 1191
Cdd:cd06174   355 ATFGLTGAFLVLA 367
MFS_SV2_like cd17316
Metazoan Synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters of the ...
 1010-1191 9.58e-06

Metazoan Synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters of the Major Facilitator Superfamily; This family is composed of metazoan synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters including those that transport inorganic phosphate (Pht), aromatic compounds (PcaK and related proteins), proline/betaine (ProP), alpha-ketoglutarate (KgtP), citrate (CitA), shikimate (ShiA), and cis,cis-muconate (MucK), among others. SV2 is a transporter-like protein that serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. Also included in this family is synaptic vesicle 2 (SV2)-related protein (SVOP) and similar proteins. SVOP is a transporter-like nucleotide binding protein that localizes to neurotransmitter-containing vesicles. The SV2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340874 [Multi-domain]  Cd Length: 353  Bit Score: 49.14  E-value: 9.58e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1010 YTLYAHKYLIISLcILNFLFNFlyiSFFIIYNIFIYDIKSSQNFYKMNDSFIILnsIILIFYIFLYFylhrIILKILNLF 1089
Cdd:cd17316   171 LALLLRRRTLLLI-LLWFFISF---GYYGLTTFLPTYLQTVLGLSPATSSLYLL--LISLGALVGAL----IAGLLSDRI 240

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1090 GYLLITFVSFSLILFLYSTSYFHISLYDNTYIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFV-- 1167
Cdd:cd17316   241 GRKKTLVIGLILSGILALPLFYLLSGSPTLLLLLLFILSFFVGGVWGALYAYLAELFPTEVRATGVGLSYNLGRLGGGga 320

                         170       180
                  ....*....|....*....|....
gi 124805449 1168 SYTLFRFIIQEQNMTLYNLIFSCV 1191
Cdd:cd17316   321 PPLIALLLASTGGTGVPALILALL 344
MFS_1 pfam07690
Major Facilitator Superfamily;
1018-1167 3.93e-04

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 44.33  E-value: 3.93e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449  1018 LIISLCILNFLFNFLYISFFIIYnIFIYDIKSSQNFYkmndSFIILNSIILIFYIFLYFYLHRIILKILNLFGYLLITFV 1097
Cdd:pfam07690  204 WLLLALLLFGFAFFGLLTYLPLY-QEVLGLSALLAGL----LLGLGGLLGAIGRLLLGRLSDRLGRRRRLLLALLLLILA 278

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449  1098 SFSLILFLYSTSYFhislydnTYIIYTIIFYLFLFIPNVSLFLFSINYFHTIHKGLLLGVFTFIGNLGFV 1167
Cdd:pfam07690  279 ALGLLLLSLTLSSL-------WLLLALLLLGFGFGLVFPALNALVSDLAPKEERGTASGLYNTAGSLGGA 341
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 1022-1166 3.35e-03

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 41.44  E-value: 3.35e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124805449 1022 LCILNFLFNFLYISFFIIYNIFIYDIKssQNFYKMNDSFIILNSI---ILIFYIFLYFYLHRIILKILNLFGYLLITFVS 1098
Cdd:cd17332   223 FLILLLAYLLYFLAFNIVNTVLVYYFK--YVLGGRAELVLLLLLIlsgALLALLPWPPLKKRFGKKKAFFIGLLLAILGL 300

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 124805449 1099 fsliLFLYSTSYFHISLYDNTYIIYTIIFYLFLFIPnVSLFLFSINY--FHTIHK--GLLLGVFTFIGNLGF 1166
Cdd:cd17332   301 ----LLLFFLPPGNLVLILVLAVLAGIGYGGANLLP-WAMLADVIDYgeLKTGKRreGIFYSVMTFFRKLGL 367
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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