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Conserved domains on  [gi|2024435455|ref|XP_004949426|]
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Krueppel-like factor 4 isoform X1 [Gallus gallus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-375 2.28e-117

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


:

Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 347.07  E-value: 2.28e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455   7 ECEMALSGTLLPSIATFAAGAAGREKAPRPAGPGNRWREELSQLKRPPPALTARPAepppsdlETAPALGPRRETEEFNE 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAVKEKALGQGSANNRWREELSHLKRPCPPLGGRCY-------DQDPPLGMRRKEEEFND 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  87 LLDFDFILSNSLMHQEpaaaaaVVVSPAAAPAPAASPCPAGPFAYPLPCAEPGGLLYGGGGgggggrdgapaaAPFNLAD 166
Cdd:cd21582    74 LLDYDFILSNSLLQQQ------QQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPI------------PRANLAD 135

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 167 ISDVSPSGGFVAELMRPDLDPVYlpapaalPPLGSLQGKFVVKAVGDYSHPGLSPKSGPptaaQPPGSEGPGAPYSPLqP 246
Cdd:cd21582   136 INDVSPSGGFVAELMRPDLDPAY-------LQPTSLHGKFVVKTTMDMGDYSQSINVSK----SAPMTKSSVAPSSSL-P 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 247 RMCPKIKQEASPPCTLAPPPHGSGprGPPPQHDFSMGRLLPARTTPSLGPEEM-------LSSRDCLPPTPGLGHPPLPL 319
Cdd:cd21582   204 FMCPRIKQENPSTCTISRPMDGHL--GGNSQHGFSQRAPLPSRTTPSGGPGGGnsstaesLMSRDHHPSSQVLSHPPLPL 281

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2024435455 320 PPGYPPAPGYPTFLPEQLPPgtLQYQELMPPGSCLPEETKPKRGRRSWPRKRTATH 375
Cdd:cd21582   282 PQGYHPSPGYPPFPPPPSQP--QQYQELMSPGSCLPEEPKPKRGRRSWPRKRTATH 335
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
382-437 2.15e-05

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 46.61  E-value: 2.15e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 382 CGKTYTKSSHLKAHLRT--HTGE--KPYHCDWEGCGWKFARSDELTRHYRKHTGHRPFQC 437
Cdd:COG5048   295 CNISFSRSSPLTRHLRSvnHSGEslKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE 354
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 435-457 5.12e-05

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


:

Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 39.98  E-value: 5.12e-05
                          10        20
                  ....*....|....*....|...
gi 2024435455 435 FQCQRCDRAFSRSDHLALHMKRH 457
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-375 2.28e-117

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 347.07  E-value: 2.28e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455   7 ECEMALSGTLLPSIATFAAGAAGREKAPRPAGPGNRWREELSQLKRPPPALTARPAepppsdlETAPALGPRRETEEFNE 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAVKEKALGQGSANNRWREELSHLKRPCPPLGGRCY-------DQDPPLGMRRKEEEFND 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  87 LLDFDFILSNSLMHQEpaaaaaVVVSPAAAPAPAASPCPAGPFAYPLPCAEPGGLLYGGGGgggggrdgapaaAPFNLAD 166
Cdd:cd21582    74 LLDYDFILSNSLLQQQ------QQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPI------------PRANLAD 135

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 167 ISDVSPSGGFVAELMRPDLDPVYlpapaalPPLGSLQGKFVVKAVGDYSHPGLSPKSGPptaaQPPGSEGPGAPYSPLqP 246
Cdd:cd21582   136 INDVSPSGGFVAELMRPDLDPAY-------LQPTSLHGKFVVKTTMDMGDYSQSINVSK----SAPMTKSSVAPSSSL-P 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 247 RMCPKIKQEASPPCTLAPPPHGSGprGPPPQHDFSMGRLLPARTTPSLGPEEM-------LSSRDCLPPTPGLGHPPLPL 319
Cdd:cd21582   204 FMCPRIKQENPSTCTISRPMDGHL--GGNSQHGFSQRAPLPSRTTPSGGPGGGnsstaesLMSRDHHPSSQVLSHPPLPL 281

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2024435455 320 PPGYPPAPGYPTFLPEQLPPgtLQYQELMPPGSCLPEETKPKRGRRSWPRKRTATH 375
Cdd:cd21582   282 PQGYHPSPGYPPFPPPPSQP--QQYQELMSPGSCLPEEPKPKRGRRSWPRKRTATH 335
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
382-437 2.15e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 46.61  E-value: 2.15e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 382 CGKTYTKSSHLKAHLRT--HTGE--KPYHCDWEGCGWKFARSDELTRHYRKHTGHRPFQC 437
Cdd:COG5048   295 CNISFSRSSPLTRHLRSvnHSGEslKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE 354
zf-H2C2_2 pfam13465
Zinc-finger double domain;
421-446 3.01e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.82  E-value: 3.01e-05
                          10        20
                  ....*....|....*....|....*.
gi 2024435455 421 ELTRHYRKHTGHRPFQCQRCDRAFSR 446
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 435-457 5.12e-05

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 39.98  E-value: 5.12e-05
                          10        20
                  ....*....|....*....|...
gi 2024435455 435 FQCQRCDRAFSRSDHLALHMKRH 457
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
BimA_second NF040983
trimeric autotransporter actin-nucleating factor BimA; This HMM describes BimA (Burkholderia ...
 203-293 1.72e-04

trimeric autotransporter actin-nucleating factor BimA; This HMM describes BimA (Burkholderia intracellular motility A), WP_004266405.1-like proteins in Burkholderia mallei or B. pseudomallei. The term BimA has also been used for WP_011205626.1-like homologs that have a very different N-terminal half.


Pssm-ID: 468913 [Multi-domain]  Cd Length: 382  Bit Score: 43.74  E-value: 1.72e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 203 QGKFVVKAVGDYSHPGLSPKSGPPTAAQPPGSEGPGAPYSPLQPrmcpkikqeaSPPCTLAPPPHGSGPRGPPPQHDFSM 282
Cdd:NF040983   72 KGDFKLKPVGDRTLPNKVPPPPPPPPPPPPPPPTPPPPPPPPPP----------PPPPSPPPPPPPSPPPSPPPPTTTPP 141

                          90
                  ....*....|.
gi 2024435455 283 GRLLPARTTPS 293
Cdd:NF040983  142 TRTTPSTTTPT 152
ZnF_C2H2 smart00355
zinc finger;
435-457 9.65e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 33.59  E-value: 9.65e-03
                           10        20
                   ....*....|....*....|...
gi 2024435455  435 FQCQRCDRAFSRSDHLALHMKRH 457
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-375 2.28e-117

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 347.07  E-value: 2.28e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455   7 ECEMALSGTLLPSIATFAAGAAGREKAPRPAGPGNRWREELSQLKRPPPALTARPAepppsdlETAPALGPRRETEEFNE 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAVKEKALGQGSANNRWREELSHLKRPCPPLGGRCY-------DQDPPLGMRRKEEEFND 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  87 LLDFDFILSNSLMHQEpaaaaaVVVSPAAAPAPAASPCPAGPFAYPLPCAEPGGLLYGGGGgggggrdgapaaAPFNLAD 166
Cdd:cd21582    74 LLDYDFILSNSLLQQQ------QQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPI------------PRANLAD 135

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 167 ISDVSPSGGFVAELMRPDLDPVYlpapaalPPLGSLQGKFVVKAVGDYSHPGLSPKSGPptaaQPPGSEGPGAPYSPLqP 246
Cdd:cd21582   136 INDVSPSGGFVAELMRPDLDPAY-------LQPTSLHGKFVVKTTMDMGDYSQSINVSK----SAPMTKSSVAPSSSL-P 203

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 247 RMCPKIKQEASPPCTLAPPPHGSGprGPPPQHDFSMGRLLPARTTPSLGPEEM-------LSSRDCLPPTPGLGHPPLPL 319
Cdd:cd21582   204 FMCPRIKQENPSTCTISRPMDGHL--GGNSQHGFSQRAPLPSRTTPSGGPGGGnsstaesLMSRDHHPSSQVLSHPPLPL 281

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2024435455 320 PPGYPPAPGYPTFLPEQLPPgtLQYQELMPPGSCLPEETKPKRGRRSWPRKRTATH 375
Cdd:cd21582   282 PQGYHPSPGYPPFPPPPSQP--QQYQELMSPGSCLPEEPKPKRGRRSWPRKRTATH 335
KLF2_N cd21583
N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as ...
 13-375 6.59e-23

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.


Pssm-ID: 409229 [Multi-domain]  Cd Length: 299  Bit Score: 98.57  E-value: 6.59e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  13 SGTLLPSIATFAAGaagREKAPRpagpgNRWREELSQLkrpppaltarPAEPPPSDLETAPALGPRRETEEFNELLDFDF 92
Cdd:cd21583     1 SGTILPSISTFANQ---KEKCWE-----DRWKGEEDKS----------SLSSCLLDLQNGDSCLGRKDDEDLDNYLDLDF 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  93 ILSNSLMHQEPAAAAAVVVSPAAAPAPAA-SPCPAGPFAYPLPCAEPGgllygggggggggrdgapaaapfnladisdvs 171
Cdd:cd21583    63 ILANTAGSDSAGAAGGGYYGSLPEPAAPYsASNPPGAYSVPEQGSPPP-------------------------------- 110

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 172 PSGGFVAELMRPDLDPVYLPApaalpplGSLQGKFVVKAVGDYSHP-GLSPKSGPPTAAQPPgSEGPGapysplqPRMCP 250
Cdd:cd21583   111 PYSSSLMAELLRDSDSDYGPS-------NSIQGRFLVSSAFFPRQDdPDSIKVEPSMDSYGP-VMGMV-------PQSCP 175

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 251 KIKQEASPPCTLAPPPhgsgPR-GPPPQHDFSMgrllpartTPSLGPEEMLSSrDCLPPTPGLGHPPLPLPPGYPPAPGY 329
Cdd:cd21583   176 KIKQEGNVSCMMSFEQ----PRlANSPQAAGSM--------TPPLSPDDLMAS-ECQQQMMCSPSFPQPHHPHYPPPPQP 242

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2024435455 330 PTFLPEQLPPG----------TLQYQELMPPGSCL-PEETKPKRGRRSWPRKRTATH 375
Cdd:cd21583   243 PLQYQSPHQFGlfedglplqpSAQRVLLTPPSSPLeLLESKPKRGRRSWPRKRTATH 299
KLF1_2_4_N-like cd22056
N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of ...
 16-375 6.56e-08

N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of Kruppel-like factor (KLF)1, KLF2, and KLF4; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domains of an unknown subfamily of KLFs, predominantly found in fish, related to the N-terminal domains of KLF1, KLF2, and KLF4.


Pssm-ID: 409231 [Multi-domain]  Cd Length: 339  Bit Score: 54.28  E-value: 6.56e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  16 LLPSIATFAAGAAGREKAPRPAgpgNRWREelSQLKRPPPALTAR-PAEPPPSDLETAPALgprRETEEFNELLDFDFIL 94
Cdd:cd22056     2 MLPSISTFASLQPLEEKQPEIL---CRWSV--DAASSEVEFSIVQsPIEVPKDDDELGKFL---DEEDILSNFLDLEFIL 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455  95 SNSlmhqepaaaaavvvspaaaPAPAASPCPAGPFAYPLP-----CAEPGGLlygggggggggrDGAPAAAPFNLADIS- 168
Cdd:cd22056    74 SSS-------------------NGSSSGPSQQQQCAYPLPespesCSTGSDS------------DGSDAPQPYHGSAGFt 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 169 DVSPSGGFVAELMRPDLdpvylpapaalPPLGSLQGKFVVKAVGDYSHPGLSPKSGPPTAAQPPGSEGPgapysplqprM 248
Cdd:cd22056   123 SSSPVHSLMAELLTPEM-----------NYPGESSPDSAGKARDGREYTELRALPTAPPAHQPATSPPP----------L 181

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 249 CPKIKQEAS-PPCTLAPPPHGSG----PRGPPPQHDFSMGRLLPARTTPSLGPEEMLSSRDCLPPTPGLGHPPLPLPPGY 323
Cdd:cd22056   182 GYKIKTEPVeQSCMMAAGGGGFMgqqkPKHQMHSVHPQAFTHHQAAGPGALQGRGGRGGPDCHLLHSSHHHHHHHHLQYQ 261

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 324 PPAPGYPTFLPEQLPPgtlQYQEL-------------MPPGSCL----------------PEETKPKRGRRSWPRKRTAT 374
Cdd:cd22056   262 YMNAPYPPHYAHQGAP---QFHGQysvfrepmrvhhqGHPGSMLtppssppllefyaqdePEDIKPKRGRRSWARKRTAT 338


                  .
gi 2024435455 375 H 375
Cdd:cd22056   339 H 339
KLF1_2_4_N cd21972
N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel ...
 166-375 5.81e-07

N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the related N-terminal domains of KLF1, KLF2, KLF4, and similar proteins.


Pssm-ID: 409230 [Multi-domain]  Cd Length: 194  Bit Score: 49.98  E-value: 5.81e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 166 DISDVSPSGGFVAELMRPDLDPVYLPAPAALPPLGSlqgkfvvkAVGDYSHPGLSPKSGPPTAAQPPGSEGPGAPysplq 245
Cdd:cd21972    23 DLEFILSNTVTSDNDNPPPPDPAYPPPESPESCSTV--------YDSDGCHPTPNAYCGPNGPGLPGHFLLAGNS----- 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 246 PRMCPKIKQEASPPCT--LAPPPHGSGPRgpPPQHDFSMgrllparttpslgpeemlssrdclPPTPGLGHPPLPLPPGY 323
Cdd:cd21972    90 PNLGPKIKTENQEQACmpVAGYSGHYGPR--EPQRVPPA------------------------PPPPQYAGHFQYHGHFN 143

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2024435455 324 PPAPGYPTFLPEQLPPGTlQYQELMPPGSCLPEETKPKRGRRSWPRKRTATH 375
Cdd:cd21972   144 MFSPPLRANHPGMSTVML-TPLSTPPLGFLSPEEAKPKRGRRSWARKRTATH 194
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
382-437 2.15e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 46.61  E-value: 2.15e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 382 CGKTYTKSSHLKAHLRT--HTGE--KPYHCDWEGCGWKFARSDELTRHYRKHTGHRPFQC 437
Cdd:COG5048   295 CNISFSRSSPLTRHLRSvnHSGEslKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKE 354
zf-H2C2_2 pfam13465
Zinc-finger double domain;
421-446 3.01e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.82  E-value: 3.01e-05
                          10        20
                  ....*....|....*....|....*.
gi 2024435455 421 ELTRHYRKHTGHRPFQCQRCDRAFSR 446
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
370-453 3.77e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 45.84  E-value: 3.77e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 370 KRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWEGCGWKFARSDELTRHYRKHTGHRPFQCQrCDRAFSRSDH 449
Cdd:COG5048   382 KNDKKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCS-ILKSFRRDLD 460


                  ....
gi 2024435455 450 LALH 453
Cdd:COG5048   461 LSNH 464
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 435-457 5.12e-05

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 39.98  E-value: 5.12e-05
                          10        20
                  ....*....|....*....|...
gi 2024435455 435 FQCQRCDRAFSRSDHLALHMKRH 457
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
BimA_second NF040983
trimeric autotransporter actin-nucleating factor BimA; This HMM describes BimA (Burkholderia ...
 203-293 1.72e-04

trimeric autotransporter actin-nucleating factor BimA; This HMM describes BimA (Burkholderia intracellular motility A), WP_004266405.1-like proteins in Burkholderia mallei or B. pseudomallei. The term BimA has also been used for WP_011205626.1-like homologs that have a very different N-terminal half.


Pssm-ID: 468913 [Multi-domain]  Cd Length: 382  Bit Score: 43.74  E-value: 1.72e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2024435455 203 QGKFVVKAVGDYSHPGLSPKSGPPTAAQPPGSEGPGAPYSPLQPrmcpkikqeaSPPCTLAPPPHGSGPRGPPPQHDFSM 282
Cdd:NF040983   72 KGDFKLKPVGDRTLPNKVPPPPPPPPPPPPPPPTPPPPPPPPPP----------PPPPSPPPPPPPSPPPSPPPPTTTPP 141

                          90
                  ....*....|.
gi 2024435455 283 GRLLPARTTPS 293
Cdd:NF040983  142 TRTTPSTTTPT 152
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 375-399 1.94e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 38.44  E-value: 1.94e-04
                          10        20
                  ....*....|....*....|....*
gi 2024435455 375 HTCDYagCGKTYTKSSHLKAHLRTH 399
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
382-430 5.82e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 41.99  E-value: 5.82e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 2024435455 382 CGKTYTKSSHLKAHLRTHTGEKPYHCDWEGCGWKFARSDELTRHYRKHT 430
Cdd:COG5048    39 CTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHH 87
zf-H2C2_2 pfam13465
Zinc-finger double domain;
391-418 1.47e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 35.81  E-value: 1.47e-03
                          10        20
                  ....*....|....*....|....*...
gi 2024435455 391 HLKAHLRTHTGEKPYHCDweGCGWKFAR 418
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCP--ECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 405-429 3.05e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.97  E-value: 3.05e-03
                          10        20
                  ....*....|....*....|....*
gi 2024435455 405 YHCdwEGCGWKFARSDELTRHYRKH 429
Cdd:pfam00096   1 YKC--PDCGKSFSRKSNLKRHLRTH 23
KLF1_N cd21581
N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as ...
 336-375 4.49e-03

N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as Krueppel-like factor 1 or Erythroid Kruppel-like Factor/EKLF) was the first Kruppel-like factor discovered. It was found to be vitally important for embryonic erythropoiesis in promoting the switch from fetal hemoglobin (Hemoglobin F) to adult hemoglobin (Hemoglobin A) gene expression by binding to highly conserved CACCC domains. EKLF ablation in mouse embryos produces a lethal anemic phenotype, causing death by embryonic day 14, and natural mutations lead to beta+ thalassemia in humans. However, expression of embryonic hemoglobin and fetal hemoglobin genes is normal in EKLF-deficient mice, suggesting other factors may be involved. KLF1 functions as a transcriptional activator. It belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF1, which is related to the N-terminal domains of KLF2 and KLF4.


Pssm-ID: 409227 [Multi-domain]  Cd Length: 278  Bit Score: 38.87  E-value: 4.49e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 2024435455 336 QLPPGTLQYQELMPPGSCLPEET-KPKRGRRSWPRKRTATH 375
Cdd:cd21581   238 PGPGHLPLGQQPLLPDPPLPPGGaEGKRGRRSWAKKRPAVH 278
ZnF_C2H2 smart00355
zinc finger;
435-457 9.65e-03

zinc finger;


Pssm-ID: 197676  Cd Length: 23  Bit Score: 33.59  E-value: 9.65e-03
                           10        20
                   ....*....|....*....|...
gi 2024435455  435 FQCQRCDRAFSRSDHLALHMKRH 457
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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