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Conserved domains on  [gi|568984531|ref|XP_006517743|]
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DNA polymerase kappa isoform X2 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PolY_Pol_IV_kappa cd03586
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ...
 38-448 1.50e-149

DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.


:

Pssm-ID: 176459 [Multi-domain]  Cd Length: 334  Bit Score: 440.42  E-value: 1.50e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKE 114
Cdd:cd03586    1 IHIDMDAFYASVEQRDNPELKGKPVAVGGSSdrgVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQ 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 115 VKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfedsppdl 194
Cdd:cd03586   81 IMEILREYTPLVEPLSIDEAYLDVT------------------------------------------------------- 105

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 195 qpqgspfqlnseeqnnpqiaqNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSrs 274
Cdd:cd03586  106 ---------------------DYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPE-- 162

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 275 AVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVER 354
Cdd:cd03586  163 DVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDRERKSIGVER 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 355 TFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEvnvg 433
Cdd:cd03586  243 TFSEdLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLEEL---- 318

                        410
                 ....*....|....*
gi 568984531 434 sPHPLRLRLMGVRMS 448
Cdd:cd03586  319 -LDGRPIRLLGVRLS 332
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 555-580 2.08e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


:

Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 41.82  E-value: 2.08e-05
                           10        20
                   ....*....|....*....|....*.
gi 568984531   555 FICPVCFREqegVSLEAFNEHVDECL 580
Cdd:smart00734   2 VQCPVCFRE---VPENLINSHLDSCL 24
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 696-722 6.43e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


:

Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 40.28  E-value: 6.43e-05
                           10        20
                   ....*....|....*....|....*..
gi 568984531   696 ALVCPVCNLEQetsDLTLFNIHVDICL 722
Cdd:smart00734   1 LVQCPVCFREV---PENLINSHLDSCL 24
 
Name Accession Description Interval E-value
PolY_Pol_IV_kappa cd03586
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ...
 38-448 1.50e-149

DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.


Pssm-ID: 176459 [Multi-domain]  Cd Length: 334  Bit Score: 440.42  E-value: 1.50e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKE 114
Cdd:cd03586    1 IHIDMDAFYASVEQRDNPELKGKPVAVGGSSdrgVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQ 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 115 VKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfedsppdl 194
Cdd:cd03586   81 IMEILREYTPLVEPLSIDEAYLDVT------------------------------------------------------- 105

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 195 qpqgspfqlnseeqnnpqiaqNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSrs 274
Cdd:cd03586  106 ---------------------DYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPE-- 162

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 275 AVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVER 354
Cdd:cd03586  163 DVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDRERKSIGVER 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 355 TFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEvnvg 433
Cdd:cd03586  243 TFSEdLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLEEL---- 318

                        410
                 ....*....|....*
gi 568984531 434 sPHPLRLRLMGVRMS 448
Cdd:cd03586  319 -LDGRPIRLLGVRLS 332
PRK02406 PRK02406
DNA polymerase IV; Validated
 42-456 2.59e-103

DNA polymerase IV; Validated


Pssm-ID: 235035 [Multi-domain]  Cd Length: 343  Bit Score: 321.68  E-value: 2.59e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  42 MDAFYAAVEMRDNPELKDKPIAVGS----MSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKE 117
Cdd:PRK02406   1 MDCFYAAVEMRDNPELRGKPVAVGGspgrRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIRE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 118 ILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfeDSPpdlQPQ 197
Cdd:PRK02406  81 IFRRYTDLIEPLSLDEAYLDVT-------------------------------------------------DNK---LCI 108

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 198 GSpfqlnseeqnnpqiaqnsvvfgtsAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRsaVM 277
Cdd:PRK02406 109 GS------------------------ATLIAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEE--VD 162

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 278 DFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELyQQRALLSLLfsetswHYF------LHialglgstDLAR-------- 343
Cdd:PRK02406 163 AFLATLPVEKIPGVGKVTAEKLHALGIYTCADL-QKYDLAELI------RHFgkfgrrLY--------ERARgiderpvk 227

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 344 -DGERKSMSVERTFSEISKTEEQyslCQE----LCAELAHDLQKEG--LKGRTVTIKLKNVNFEVKTRaSTVPAAISTAE 416
Cdd:PRK02406 228 pDRERKSVGVERTFAEDLYDLEA---CLAelprLAEKLERRLERAKpdKRIKTVGVKLKFADFQQTTK-EHTADPLDKAD 303

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|
gi 568984531 417 EIFAIAKELLRTEVNVGsphplrLRLMGVRMSTFSSEDDR 456
Cdd:PRK02406 304 LIELLAQALLRRLGGRG------VRLLGVGVTLLEPQLER 337
DinP COG0389
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ...
 36-448 2.28e-100

Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];


Pssm-ID: 440158 [Multi-domain]  Cd Length: 336  Bit Score: 313.62  E-value: 2.28e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  36 TIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSM---LATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVS 112
Cdd:COG0389    2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGDNNrgvVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYRDVS 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 113 KEVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpeDKRRYFikmgnylkidtprqeanelteyersispllfedspp 192
Cdd:COG0389   82 RRVMAILERYTPLVEPLSIDEAFLDVT-----------GSARLF------------------------------------ 114

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 193 dlqpqgspfqlnseeqnnpqiaqnsvvfgTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPS 272
Cdd:COG0389  115 -----------------------------GSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPG 165

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 273 RsaVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQ-RALLSLLFSEtSWHYFLHIALGLGSTDLARDGERKSMS 351
Cdd:COG0389  166 E--VAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALpRAELRRRFGK-VGERLYRLARGIDPRPVEPRRPRKSIG 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 352 VERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEV 430
Cdd:COG0389  243 VERTFGEdLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTTRSRTLPEPTDDTAELLRAARELLERIY 322

                        410
                 ....*....|....*...
gi 568984531 431 NVGSPhplrLRLMGVRMS 448
Cdd:COG0389  323 RPGRP----VRLLGVRLS 336
IMS pfam00817
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
 40-259 2.17e-46

impB/mucB/samB family; These proteins are involved in UV protection (Swiss).


Pssm-ID: 425885 [Multi-domain]  Cd Length: 148  Bit Score: 161.97  E-value: 2.17e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531   40 VDMDAFYAAVEMRDNPELKDKPIAVGS---MSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVK 116
Cdd:pfam00817   1 IDMDAFFASVELLRDPELKGKPVAVGGgngRGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRKIF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  117 EILAEY-DPNFMAMSLDEAYLnitqhlqerqdwpedkrryfikmgnylkidtprqeanELTEYERsisplLFEDSppdlq 195
Cdd:pfam00817  81 EILRRFsTPKVEQASIDEAFL-------------------------------------DLTGLEK-----LFGAE----- 113

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 568984531  196 pqgspfqlnseeqnnpqiaqnsvvfgtsaEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSD 259
Cdd:pfam00817 114 -----------------------------EALAKRLRREIAEETGLTCSIGIAPNKLLAKLASD 148
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 555-580 2.08e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 41.82  E-value: 2.08e-05
                           10        20
                   ....*....|....*....|....*.
gi 568984531   555 FICPVCFREqegVSLEAFNEHVDECL 580
Cdd:smart00734   2 VQCPVCFRE---VPENLINSHLDSCL 24
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 696-722 6.43e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 40.28  E-value: 6.43e-05
                           10        20
                   ....*....|....*....|....*..
gi 568984531   696 ALVCPVCNLEQetsDLTLFNIHVDICL 722
Cdd:smart00734   1 LVQCPVCFREV---PENLINSHLDSCL 24
 
Name Accession Description Interval E-value
PolY_Pol_IV_kappa cd03586
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ...
 38-448 1.50e-149

DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.


Pssm-ID: 176459 [Multi-domain]  Cd Length: 334  Bit Score: 440.42  E-value: 1.50e-149
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKE 114
Cdd:cd03586    1 IHIDMDAFYASVEQRDNPELKGKPVAVGGSSdrgVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPPRFDKYREVSRQ 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 115 VKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfedsppdl 194
Cdd:cd03586   81 IMEILREYTPLVEPLSIDEAYLDVT------------------------------------------------------- 105

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 195 qpqgspfqlnseeqnnpqiaqNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSrs 274
Cdd:cd03586  106 ---------------------DYVRLFGSATEIAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPE-- 162

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 275 AVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMSVER 354
Cdd:cd03586  163 DVEEFLAPLPVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVELLKKLFGKSGRRLYELARGIDNRPVEPDRERKSIGVER 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 355 TFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEvnvg 433
Cdd:cd03586  243 TFSEdLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKLKYADFSTRTRSRTLPEPTDDAEDIYELALELLEEL---- 318

                        410
                 ....*....|....*
gi 568984531 434 sPHPLRLRLMGVRMS 448
Cdd:cd03586  319 -LDGRPIRLLGVRLS 332
PRK02406 PRK02406
DNA polymerase IV; Validated
 42-456 2.59e-103

DNA polymerase IV; Validated


Pssm-ID: 235035 [Multi-domain]  Cd Length: 343  Bit Score: 321.68  E-value: 2.59e-103
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  42 MDAFYAAVEMRDNPELKDKPIAVGS----MSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKE 117
Cdd:PRK02406   1 MDCFYAAVEMRDNPELRGKPVAVGGspgrRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPGRFDVYKEVSRQIRE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 118 ILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfeDSPpdlQPQ 197
Cdd:PRK02406  81 IFRRYTDLIEPLSLDEAYLDVT-------------------------------------------------DNK---LCI 108

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 198 GSpfqlnseeqnnpqiaqnsvvfgtsAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRsaVM 277
Cdd:PRK02406 109 GS------------------------ATLIAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEE--VD 162

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 278 DFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELyQQRALLSLLfsetswHYF------LHialglgstDLAR-------- 343
Cdd:PRK02406 163 AFLATLPVEKIPGVGKVTAEKLHALGIYTCADL-QKYDLAELI------RHFgkfgrrLY--------ERARgiderpvk 227

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 344 -DGERKSMSVERTFSEISKTEEQyslCQE----LCAELAHDLQKEG--LKGRTVTIKLKNVNFEVKTRaSTVPAAISTAE 416
Cdd:PRK02406 228 pDRERKSVGVERTFAEDLYDLEA---CLAelprLAEKLERRLERAKpdKRIKTVGVKLKFADFQQTTK-EHTADPLDKAD 303

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|
gi 568984531 417 EIFAIAKELLRTEVNVGsphplrLRLMGVRMSTFSSEDDR 456
Cdd:PRK02406 304 LIELLAQALLRRLGGRG------VRLLGVGVTLLEPQLER 337
DinP COG0389
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ...
 36-448 2.28e-100

Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];


Pssm-ID: 440158 [Multi-domain]  Cd Length: 336  Bit Score: 313.62  E-value: 2.28e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  36 TIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSM---LATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVS 112
Cdd:COG0389    2 RILHVDMDAFYASVEQRDRPELRGKPVAVGGDNNrgvVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPPDFELYRDVS 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 113 KEVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpeDKRRYFikmgnylkidtprqeanelteyersispllfedspp 192
Cdd:COG0389   82 RRVMAILERYTPLVEPLSIDEAFLDVT-----------GSARLF------------------------------------ 114

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 193 dlqpqgspfqlnseeqnnpqiaqnsvvfgTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPS 272
Cdd:COG0389  115 -----------------------------GSAEAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPG 165

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 273 RsaVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQ-RALLSLLFSEtSWHYFLHIALGLGSTDLARDGERKSMS 351
Cdd:COG0389  166 E--VAAFLAPLPVEKLWGVGPKTAEKLARLGIRTIGDLAALpRAELRRRFGK-VGERLYRLARGIDPRPVEPRRPRKSIG 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 352 VERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRTEV 430
Cdd:COG0389  243 VERTFGEdLTDLEELEAALRRLAERLAERLRRQGLGARTVTVKLRTSDFRTTTRSRTLPEPTDDTAELLRAARELLERIY 322

                        410
                 ....*....|....*...
gi 568984531 431 NVGSPhplrLRLMGVRMS 448
Cdd:COG0389  323 RPGRP----VRLLGVRLS 336
PTZ00205 PTZ00205
DNA polymerase kappa; Provisional
 12-472 5.86e-72

DNA polymerase kappa; Provisional


Pssm-ID: 140232 [Multi-domain]  Cd Length: 571  Bit Score: 246.08  E-value: 5.86e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  12 QLRKAQLQVDKFAMELERNRNLNnTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIA 91
Cdd:PTZ00205 111 QRKQWELKVSKIEQELEATRRLG-TYIHLDMDMFYAAVEIKKHPEYAAIPLAIGTMTMLQTANYVARGRGIRQGMPGFLA 189

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  92 KRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQErqdwpedkrryfikmgnylkidtprqe 171
Cdd:PTZ00205 190 LKICPNLLILPPDFDAYNEESNTVRRIVAEYDPNYISFGLDELTLEVSAYIER--------------------------- 242

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 172 anelteyersispllFEdsppdlqpqgspfqlnseeqnnpqiaqnsvvfGT-SAEEVVKEIRFRIEQKTTLTASAGIAPN 250
Cdd:PTZ00205 243 ---------------FE--------------------------------GTkTAEDVASELRVRVFGETKLTASAGIGPT 275

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 251 TMLAKVCSDKNKPNGQYQI-LPSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYF 329
Cdd:PTZ00205 276 AALAKIASNINKPNGQHDLnLHTRGDVMTYVRDLGLRSVPGVGKVTEALLKGLGITTLSDIYNRRVELCYILHNNLFRFL 355

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 330 LHIALGL------------GSTDLARDGERKSMSVERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNV 397
Cdd:PTZ00205 356 LGASIGImqwpdaataantENCEGATGGQRKAISSERSFTTPRTKEGLQEMVDTVFNGAYEEMRKSELMCRQISLTIRWA 435

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 568984531 398 NFEVKTRASTVPAAISTAEEIFAIAKELLRtevnvgsPHPLR---LRLMGVRMSTFSSEDDRkHQQRsiigflQAGNQ 472
Cdd:PTZ00205 436 SYRYQQYTKSLIQYSDDSATLRRAVDGLLL-------PHAAKyseMCLLGVRFLDLISAKDF-HMKR------KGGNQ 499
PRK14133 PRK14133
DNA polymerase IV; Provisional
 33-463 4.91e-69

DNA polymerase IV; Provisional


Pssm-ID: 184529 [Multi-domain]  Cd Length: 347  Bit Score: 231.14  E-value: 4.91e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  33 LNNTIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYR 109
Cdd:PRK14133   1 MDRVIIHVDMDAFFASVEQMDNPKLKGKPVIVGGISergVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVRHERYK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 110 AVSKEVKEILAEYDPNFMAMSLDEAYLNITQhlqerqdwpedkrryfikmgnylkIDtprqeanelteyersispllfed 189
Cdd:PRK14133  81 EVSKNIFKILYEVTPIVEPVSIDEAYLDITN------------------------IK----------------------- 113

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 190 sppdlqpqgspfqlnseeqNNPqiaqnsvvfgtsaEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQI 269
Cdd:PRK14133 114 -------------------EEP-------------IKIAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKII 161

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 270 lpSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQ-QRALLSLLFSETSWHYFLHIAlGLGSTDLARDGERK 348
Cdd:PRK14133 162 --TEDMIPDILKPLPISKVHGIGKKSVEKLNNIGIYTIEDLLKlSREFLIEYFGKFGVEIYERIR-GIDYREVEVSRERK 238

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 349 SMSVERTFS-EISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLR 427
Cdd:PRK14133 239 SIGKETTLKkDTKDKEELKKYLKDFSNIISEELKKRNLYGKTVTVKIKTSDFQTHTKSKTLNDYIRDKEEIYNVACEILE 318

                        410       420       430
                 ....*....|....*....|....*....|....*.
gi 568984531 428 tEVNVGSPhplrLRLMGVRMSTFSSEddrKHQQRSI 463
Cdd:PRK14133 319 -HINIKEP----IRLIGLSVSNLSEN---KIEQLSF 346
PRK02794 PRK02794
DNA polymerase IV; Provisional
 36-455 1.91e-67

DNA polymerase IV; Provisional


Pssm-ID: 179473 [Multi-domain]  Cd Length: 419  Bit Score: 229.43  E-value: 1.91e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  36 TIVHVDMDAFYAAVEMRDNPELKDKPIAVG--SMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSK 113
Cdd:PRK02794  37 SIAHIDCDAFYASVEKRDNPELRDKPVIIGggKRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKPDMEKYVRVGR 116

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 114 EVKEILAEYDPNFMAMSLDEAYLnitqhlqerqdwpedkrryfikmgnylkidtprqeanELTEYERsisplLFEDSPpd 193
Cdd:PRK02794 117 EVRAMMQALTPLVEPLSIDEAFL-------------------------------------DLSGTER-----LHGAPP-- 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 194 lqpqgspfqlnseeqnnpqiaqnSVVFgtsaeevvkeIRF--RIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGqYQILp 271
Cdd:PRK02794 153 -----------------------AVVL----------ARFarRVEREIGITVSVGLSYNKFLAKIASDLDKPRG-FSVI- 197

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 272 SRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQ--QRALLSLLFSETSWHYflHIALGLGSTDLARDGERKS 349
Cdd:PRK02794 198 GRAEALAFLAPKPVGIIWGVGPATAARLARDGIRTIGDLQRadEADLMRRFGSMGLRLW--RLARGIDDRKVSPDREAKS 275

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 350 MSVERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLRT 428
Cdd:PRK02794 276 VSAETTFETdLSDFEDLEPILWRLSEKVSRRLKAAGLAGRTVTLKLKTADFRLRTRRRTLEDPTQLADRIFRTARELLEK 355

                        410       420
                 ....*....|....*....|....*..
gi 568984531 429 EvnvgsPHPLRLRLMGVRMSTFSSEDD 455
Cdd:PRK02794 356 E-----TDGTAFRLIGIGVSDLSPADE 377
PRK03348 PRK03348
DNA polymerase IV; Provisional
 36-452 6.17e-61

DNA polymerase IV; Provisional


Pssm-ID: 235118 [Multi-domain]  Cd Length: 454  Bit Score: 212.49  E-value: 6.17e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  36 TIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNYHARRFGVRAAMPGFIAKRLCPQL-IIVPPNFDKYRAV 111
Cdd:PRK03348   6 WVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGgrgVVAGASYEARVFGARSAMPMHQARRLVGNGaVVLPPRFVVYRAA 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 112 SKEVKEILAEYDPNFMAMSLDEAYLNitqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfedsP 191
Cdd:PRK03348  86 SRRVFDTLRELSPVVEQLSFDEAFVE-----------------------------------------------------P 112

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 192 PDLQpqgspfqlnseeqnnpqiaqnsvvfGTSAEEVVK---EIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQ 268
Cdd:PRK03348 113 AELA-------------------------GASAEEVEAfaeRLRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIRV 167

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 269 ILPSRSavMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQ--QRALLSLLfSETSWHYFLHIALGLGSTDLARDGE 346
Cdd:PRK03348 168 VPPGEE--RELLAPLPVRRLWGIGPVTEEKLHRLGIETIGDLAAlsEAEVANLL-GATVGPALHRLARGIDDRPVAERAE 244

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 347 RKSMSVERTFSEISKTEEQYSLCQELCAELAHD-LQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKEL 425
Cdd:PRK03348 245 AKQISAESTFAVDLTTRAQLREAIERIAEHAHRrLLKDGRGARTVTVKLRKSDFSTLTRSATLPYATDDAAVLAATARRL 324

                        410       420
                 ....*....|....*....|....*..
gi 568984531 426 LRTEVNVGSphplrLRLMGVRMSTFSS 452
Cdd:PRK03348 325 LLDPDEIGP-----IRLVGVGFSGLSD 346
PRK01810 PRK01810
DNA polymerase IV; Validated
 37-502 6.09e-53

DNA polymerase IV; Validated


Pssm-ID: 179337 [Multi-domain]  Cd Length: 407  Bit Score: 189.09  E-value: 6.09e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  37 IVHVDMDAFYAAVEMRDNPELKDKPIAV-GSMS----MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAV 111
Cdd:PRK01810   7 IFHVDMNSFFASVEIAYDPSLQGKPLAVaGNEKerkgIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRRPNFDRYREA 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 112 SKEVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkiDTPrqeanelteyeRSISPLlfedsp 191
Cdd:PRK01810  87 SRQMFQILSEFTPLVQPVSIDEGYLDIT--------------------------DCY-----------ALGSPL------ 123

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 192 pdlqpqgspfqlnseeqnnpqiaqnsvvfgtsaeEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGqYQILP 271
Cdd:PRK01810 124 ----------------------------------EIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLG-ITVLR 168

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 272 SRSaVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQ-QRALLSLLFSETSWHyfLH-IALGL--GSTDLARDGER 347
Cdd:PRK01810 169 KRD-VPEMLWPLPVGEMHGIGEKTAEKLKDIGIQTIGDLAKaDEHILRAKLGINGVR--LQrRANGIddRPVDPEAIYQF 245

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 348 KSMSVERTFSEISKTE-EQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELL 426
Cdd:PRK01810 246 KSVGNSTTLSHDMDEEkELLDVLRRLSKSVSKRLQKKTVVSYNVQIMIRYHDRRTITRSKTLKNPIWEKRDIFQAASRLF 325

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 427 RTEVNvGSPhplrLRLMGV-------------RMSTFSSEDDRKHQQ-RSIIGFLQA--GNQALSSTGDSLDKTDKTEla 490
Cdd:PRK01810 326 KQHWN-GDP----VRLLGVtatdlewkteavkQLDLFSFEEDAKEEPlLAVIDQINDkyGMPLLQRGSQLLRKQEKTF-- 398

                        490
                 ....*....|..
gi 568984531 491 kplEMSHKKSFF 502
Cdd:PRK01810 399 ---GTSFEKDFM 407
PolY cd00424
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ...
 38-448 7.37e-53

Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.


Pssm-ID: 176453 [Multi-domain]  Cd Length: 343  Bit Score: 187.18  E-value: 7.37e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAV----GSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSK 113
Cdd:cd00424    1 LHIDFDNFFASVEQLARPELKGRPVVVvpfnSDSTCVIACSYEARKYGVKRGMPVREARKMCPNLILVPARLDLYRRLSE 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 114 EVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfedsppd 193
Cdd:cd00424   81 RLLSELEEVAPLVEVASIDELFLDLT------------------------------------------------------ 106

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 194 lqpqGSPFQLnseeqnnpqiaqnsvvfgTSAEEVVKEIRFRIEQKT-TLTASAGIAPNTMLAKVCSDKNKPNGQYQILPs 272
Cdd:cd00424  107 ----GSARLL------------------GLGSEVALRIKRHIAEQLgGITASIGIASNKLLAKLAAKYAKPDGLTILDP- 163

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 273 rSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELY--QQRALLSLLFSETS--WHYFLHialGLGSTDLARDGERK 348
Cdd:cd00424  164 -EDLPGFLSKLPLTDLPGIGAVTAKRLEAVGINPIGDLLaaSPDALLALWGGVSGerLWYALR---GIDDEPLSPPRPRK 239

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 349 SMSVERTFSEISKTEEQ-YSLCQELCAELAHDLQKEGLKGRTVTIKLKNVN------FEVKTRASTVPAAISTaEEIFAI 421
Cdd:cd00424  240 SFSHERVLPRDSRNAEDaRPLLRLLLEKLARRLRRDGRGATRLRLWLRTVDgrwsghADIPSRSAPRPISTED-GELLHA 318

                        410       420
                 ....*....|....*....|....*..
gi 568984531 422 AKELLRTEVNVgsPHPLRLRLMGVRMS 448
Cdd:cd00424  319 LDKLWRALLDD--KGPRRLRRLGVRLS 343
PRK03858 PRK03858
DNA polymerase IV; Validated
 36-481 1.68e-50

DNA polymerase IV; Validated


Pssm-ID: 179663 [Multi-domain]  Cd Length: 396  Bit Score: 182.11  E-value: 1.68e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  36 TIVHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSnYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEV 115
Cdd:PRK03858   5 SILHADLDSFYASVEQRDDPALRGRPVIVGGGVVLAAS-YEAKAYGVRTAMGGRQARRLCPQAVVVPPRMSAYSRASKAV 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 116 KEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpeDKRRyfikmgnylkidtprqeanelteyersispllfedsppdlq 195
Cdd:PRK03858  84 FEVFRDTTPLVEGLSIDEAFLDVG-----------GLRR----------------------------------------- 111

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 196 pqgspfqlnseeqnnpqiaqnsvVFGTSAeEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSa 275
Cdd:PRK03858 112 -----------------------ISGTPV-QIAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRE- 166

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 276 vMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQ--QRALLSLLFSETSWHyfLHiALGlgstdLARD-------GE 346
Cdd:PRK03858 167 -LAFLHPLPVRRLWGVGPVTAAKLRAHGITTVGDVAElpESALVSLLGPAAGRH--LH-ALA-----HNRDprrvetgRR 237

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 347 RKSMSVERTFSEISKTEEQY--SLCQeLCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKE 424
Cdd:PRK03858 238 RRSVGAQRALGRGPNSPAEVdaVVVA-LVDRVARRMRAAGRTGRTVVLRLRFDDFTRATRSHTLPRPTASTATLLAAARD 316

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 568984531 425 LLRtevNVGSPHPLR-LRLMGVRMSTFsseDDRKHQQRSIIGFLQAGNQALSSTGDSL 481
Cdd:PRK03858 317 LVA---AAAPLIAERgLTLVGFAVSNL---DDDGAQQLELPFGLRRPGSALDAALDAV 368
PolY_Rev1 cd01701
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ...
 33-428 1.40e-48

DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.


Pssm-ID: 176455 [Multi-domain]  Cd Length: 404  Bit Score: 177.12  E-value: 1.40e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  33 LNNTIVHVDMDAFYAAVEMRDNPELKDKPIAV----GSMSMLATSNYHARRFGVRAAMpgFI--AKRLCPQLIIVPPNFD 106
Cdd:cd01701   45 LQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVchgkGPNSEIASCNYEARSYGIKNGM--WVgqAKKLCPQLVTLPYDFE 122

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 107 KYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQErqdwpedkrryfikmgnylkidtprqeaneltEYErsispll 186
Cdd:cd01701  123 AYEEVSLTFYEILASYTDNIEAVSCDEALIDITSLLEE--------------------------------TYE------- 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 187 fedsppdlqpqgspfqlnseeqnnpqiaqnsvvfgtSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQ 266
Cdd:cd01701  164 ------------------------------------LPEELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKPDGQ 207

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 267 YQILPsrSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQ----RALLSLLFSETSWHyFLHIALGLGSTDLA 342
Cdd:cd01701  208 YHLSA--EKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGDTCGGLELRsktkEKLQKVLGPKTGEK-LYDYCRGIDDRPVT 284

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 343 RDGERKSMSVERTFSEISKTEEQ-YSLCQELCAELAHDLQKEGLKGRTVTIKLKN------------------VNFevkT 403
Cdd:cd01701  285 GEKERKSVSAEINYGIRFTNVDDvEQFLQRLSEELSKRLEESNVTGRQITLKLMKrapgapieppkymghgicDSF---S 361

                        410       420
                 ....*....|....*....|....*
gi 568984531 404 RASTVPAAISTAEEIFAIAKELLRT 428
Cdd:cd01701  362 KSSTLGVATDDSGVIGTEAKKLFRD 386
IMS pfam00817
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
 40-259 2.17e-46

impB/mucB/samB family; These proteins are involved in UV protection (Swiss).


Pssm-ID: 425885 [Multi-domain]  Cd Length: 148  Bit Score: 161.97  E-value: 2.17e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531   40 VDMDAFYAAVEMRDNPELKDKPIAVGS---MSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVK 116
Cdd:pfam00817   1 IDMDAFFASVELLRDPELKGKPVAVGGgngRGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVVPPDLELYRRASRKIF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  117 EILAEY-DPNFMAMSLDEAYLnitqhlqerqdwpedkrryfikmgnylkidtprqeanELTEYERsisplLFEDSppdlq 195
Cdd:pfam00817  81 EILRRFsTPKVEQASIDEAFL-------------------------------------DLTGLEK-----LFGAE----- 113

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 568984531  196 pqgspfqlnseeqnnpqiaqnsvvfgtsaEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSD 259
Cdd:pfam00817 114 -----------------------------EALAKRLRREIAEETGLTCSIGIAPNKLLAKLASD 148
PRK03103 PRK03103
DNA polymerase IV; Reviewed
 37-463 4.70e-37

DNA polymerase IV; Reviewed


Pssm-ID: 235104 [Multi-domain]  Cd Length: 409  Bit Score: 143.99  E-value: 4.70e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  37 IVHVDMDAFYAAVEMRDNPELKDKPIAVG------SMSMLATSnYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRA 110
Cdd:PRK03103   5 ILLVDMQSFYASVEKAANPELKGRPVIVSgdperrSGVVLAAC-PLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQRYID 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 111 VSKEVKEILAEYDPNFMAMSLDEAYLNITQhlqerqdwpedkrryfikmgnylkidtprqeanelteyersiSPLLFeds 190
Cdd:PRK03103  84 VSLQITRILEDFTDLVEPFSIDEQFLDVTG------------------------------------------SQKLF--- 118

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 191 ppdlqpqGSPFqlnseeqnnpQIAQNsvvfgtsaeevvkeIRFRIEQKTTLTASAGIAPNTMLAKVCSD---KNKPNGQY 267
Cdd:PRK03103 119 -------GSPL----------EIAQK--------------IQQRIMRETGVYARVGIGPNKLLAKMACDnfaKKNPDGLF 167

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 268 QIlpSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELyqqrALLSLLFSETSW----HYFLHIALGLGSTDLAR 343
Cdd:PRK03103 168 TL--DKEDVPADLWPLPVRKLFGVGSRMEKHLRRMGIRTIGQL----ANTPLERLKKRWgingEVLWRTANGIDYSPVTP 241

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 344 DG--ERKSMSVERTF-SEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKT---RASTVPAAISTAEE 417
Cdd:PRK03103 242 HSldRQKAIGHQMTLpRDYRGFEEIKVVLLELCEEVCRRARAKGYMGRTVSVSLRGADFDWPTgfsRQMTLPEPTNLAME 321

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|...
gi 568984531 418 IFAIAKELLRTEVNvGSPhplrLRLMGVRMSTFSSED-------DRKHQQRSI 463
Cdd:PRK03103 322 VYEAACKLFHRHWD-GKP----VRRVGVTLSNLVSDDvwqlslfGDRERKRSL 369
PRK03352 PRK03352
DNA polymerase IV; Validated
 37-446 1.75e-35

DNA polymerase IV; Validated


Pssm-ID: 179564 [Multi-domain]  Cd Length: 346  Bit Score: 137.85  E-value: 1.75e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  37 IVHVDMDAFYAAVEMRDNPELKDKPIAVG-------SMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYR 109
Cdd:PRK03352   7 VLHVDLDQFIAAVELLRRPELAGLPVIVGgngdptePRKVVTCASYEARAFGVRAGMPLRTAARRCPDAVFLPSDPAAYD 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 110 AVSKEVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylkidtprqeanelteyersispllfED 189
Cdd:PRK03352  87 AASEEVMATLRDLGVPVEVWGWDEAFLGVD------------------------------------------------TD 118

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 190 SPpdlqpqgspfqlnseeqnnpqiaqnsvvfgtsaEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQI 269
Cdd:PRK03352 119 DP---------------------------------EALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRL 165

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 270 lpSRSAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTEL-YQQRALLSLLFSETSWHYFLHIALGLGSTDL-ARDGER 347
Cdd:PRK03352 166 --TDANWMAVMGDRPTDALWGVGPKTAKRLAALGITTVADLaAADPAELAATFGPTTGPWLLLLARGGGDTEVsAEPWVP 243

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 348 KSMSVERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELL 426
Cdd:PRK03352 244 RSRSREVTFPQdLTDRAEVESAVRELARRVLDEVVAEGRPVTRVAVKVRTATFYTRTKIRKLPEPTTDPDVIEAAALDVL 323

                        410       420
                 ....*....|....*....|
gi 568984531 427 rTEVNVGSPhplrLRLMGVR 446
Cdd:PRK03352 324 -DRFELDRP----VRLLGVR 338
PolY_Pol_iota cd01703
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ...
 38-313 5.84e-34

DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.


Pssm-ID: 176457 [Multi-domain]  Cd Length: 379  Bit Score: 134.13  E-value: 5.84e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVP-PNFDKYRAVSKEVK 116
Cdd:cd01703    1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNgEDLTPFRDMSKKVY 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 117 EILAEYDPNFMA--MSLDEAYLNITqhlqerqdwpeDKRryfikmgnylkidtprqeanelteyersispLLFedsppdl 194
Cdd:cd01703   81 RLLRSYSWNDRVerLGFDENFMDVT-----------EMR-------------------------------LLV------- 111

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 195 qpqGSpfqlnseeqnnpQIAQnsvvfgtsaeevvkEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILP-SR 273
Cdd:cd01703  112 ---AS------------HIAY--------------EMRERIENELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPpSC 162

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|
gi 568984531 274 SAVMDFIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQ 313
Cdd:cd01703  163 ADLMDFMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEF 202
PolY_Pol_eta cd01702
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ...
 38-427 8.47e-34

DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.


Pssm-ID: 176456 [Multi-domain]  Cd Length: 359  Bit Score: 133.21  E-value: 8.47e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  38 VHVDMDAFYAAVEMRDNPELKDKPIAVGSMSMLATSNYHARRFGVRAAMPGFIAKRLCPQLII--VPP------------ 103
Cdd:cd01702    1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNSIIAVSYAARAFGVTRFMTIDEAKKKCPDLILahVATykkgedeadyhe 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 104 ---------NFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLnitqhlqerqdwpedkrryfikmgnylkidtprqeane 174
Cdd:cd01702   81 npsparhkvSLDPYRRASRKILNILKRFGDVVEKASIDEAYL-------------------------------------- 122

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 175 lteyersispllfedsppDLqpqgspfqlnseeqnnpqiaqnsvvfgtsAEEVVKEIRFRIEQKTTLTASAGIAPNTMLA 254
Cdd:cd01702  123 ------------------DL-----------------------------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLA 155

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 255 KVCSDKNKPNGQyQILPSrSAVMDFIKDLPIRKVSGI-GKVTEKMLMALGIVTCTELYQQRALLSLL---FSET--SWHY 328
Cdd:cd01702  156 KLASGMNKPNAQ-TILRN-DAVASFLSSLPITSIRGLgGKLGEEIIDLLGLPTEGDVAGFRSSESDLqehFGEKlgEWLY 233

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 329 flHIALGLGSTDLARDGERKSMSVERTFS--EISKTEEQYSLCQELCAELAHDLQKE----GLKGRTVTIKLKNVNFEVK 402
Cdd:cd01702  234 --NLLRGIDHEPVKPRPLPKSMGSSKNFPgkTALSTEDVQHWLLVLASELNSRLEDDryenNRRPKTLVLSLRQRGDGVR 311

                        410       420
                 ....*....|....*....|....*
gi 568984531 403 TRASTvPAAISTAEEIFAIAKELLR 427
Cdd:cd01702  312 RSRSC-ALPRYDAQKIVKDAFKLIK 335
PRK01216 PRK01216
DNA polymerase IV; Validated
 37-304 3.72e-32

DNA polymerase IV; Validated


Pssm-ID: 179251 [Multi-domain]  Cd Length: 351  Bit Score: 128.37  E-value: 3.72e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  37 IVHVDMDAFYAAVEMRDNPELKDKPIAVGSMS-------MLATSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYR 109
Cdd:PRK01216   3 ILFVDFDYFFAQVEEVLNPSLKGKPVVVCVYSgrfedsgAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEVYQ 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 110 AVSKEVKEILAEYDPNFMAMSLDEAYLNITQhlqerqdwpedkrryfiKMGNYlkidtprQEANELTeyersispllfed 189
Cdd:PRK01216  83 QVSNRIMKLLREYSEKIEIASIDEAYLDISD-----------------KVKNY-------QDAYNLG------------- 125

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 190 sppdlqpqgspfqlnseeqnnpqiaqnsvvfgtsaeevvKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQI 269
Cdd:PRK01216 126 ---------------------------------------LEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVI 166

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 568984531 270 LPSRsaVMDFIKDLPIRKVSGIGKVTEKMLMALGI 304
Cdd:PRK01216 167 DDEE--VKRFINELDIADIPGIGDITAEKLKKLGV 199
PolY_Pol_V_umuC cd01700
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ...
 40-427 8.26e-27

umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.


Pssm-ID: 176454 [Multi-domain]  Cd Length: 344  Bit Score: 112.26  E-value: 8.26e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  40 VDMDAFYAAVEMRDNPELKDKPIAVGS---MSMLATSNyHARRFGVRAAMPGFIAKRLCPQL--IIVPPNFDKYRAVSKE 114
Cdd:cd01700    3 VDCNSFYASCERVFRPLLLGRPLVVLSnndGCVIARSP-EAKALGIKMGSPYFKVPDLLERHgvAVFSSNYALYGDMSRR 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 115 VKEILAEYDPNFMAMSLDEAylnitqhlqerqdwpedkrryFIKMGNYLKIDTPRqeanelteyersispllfedsppdl 194
Cdd:cd01700   82 IMSILERFSPDVEVYSIDES---------------------FLDLTGSLRFGDLE------------------------- 115

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 195 qpqgspfqlnseeqnnpqiaqnsvvfgtsaeEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSD----KNKPNGQYQIL 270
Cdd:cd01700  116 -------------------------------ELARKIRRRILQETGIPVTVGIGPTKTLAKLANDlakkKNPYGGVVDLT 164

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 271 --PSRSAVMdfiKDLPIRKVSGIGKVTEKMLMALGIVT-----CTELYQQRALLSLLFSETSWHyfLHialGLGSTDLAR 343
Cdd:cd01700  165 deEVRDKLL---KILPVGDVWGIGRRTAKKLNAMGIHTagdlaQADPDLLRKKFGVVGERLVRE--LN---GIDCLPLEE 236

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 344 -DGERKSMSVERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNF--EVKTRASTVPAAISTAE--E 417
Cdd:cd01700  237 yPPPKKSIGSSRSFGRdVTDLDELKQALAEYAERAAEKLRRQKSVARTISVFIGTSGFsrQPKYYSATNTLPYPTNDtrE 316

                        410
                 ....*....|
gi 568984531 418 IFAIAKELLR 427
Cdd:cd01700  317 IVKAALRLLY 326
IMS_C pfam11799
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
 347-454 3.22e-23

impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).


Pssm-ID: 463354 [Multi-domain]  Cd Length: 104  Bit Score: 94.93  E-value: 3.22e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  347 RKSMSVERTFSE-ISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKEL 425
Cdd:pfam11799   1 RKSIGAERTFGRdLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYSDFRTITRSVTLPSPTDDTDEIYRAALRL 80

                          90       100
                  ....*....|....*....|....*....
gi 568984531  426 LRTEVnvgspHPLRLRLMGVRMSTFSSED 454
Cdd:pfam11799  81 LRRLY-----RGRPVRLLGVSLSNLVPEG 104
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 555-580 2.08e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 41.82  E-value: 2.08e-05
                           10        20
                   ....*....|....*....|....*.
gi 568984531   555 FICPVCFREqegVSLEAFNEHVDECL 580
Cdd:smart00734   2 VQCPVCFRE---VPENLINSHLDSCL 24
IMS_HHH pfam11798
IMS family HHH motif; These proteins are involved in UV protection, eg.
 273-304 4.24e-05

IMS family HHH motif; These proteins are involved in UV protection, eg.


Pssm-ID: 432081 [Multi-domain]  Cd Length: 32  Bit Score: 40.84  E-value: 4.24e-05
                          10        20        30
                  ....*....|....*....|....*....|..
gi 568984531  273 RSAVMDFIKDLPIRKVSGIGKVTEKMLMALGI 304
Cdd:pfam11798   1 PDDVPEFLWPLPISKIPGIGKKLAEKLKALGI 32
ZnF_Rad18 smart00734
Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative ...
 696-722 6.43e-05

Rad18-like CCHC zinc finger; Yeast Rad18p functions with Rad5p in error-free post-replicative DNA repair. This zinc finger is likely to bind nucleic-acids.


Pssm-ID: 128973 [Multi-domain]  Cd Length: 24  Bit Score: 40.28  E-value: 6.43e-05
                           10        20
                   ....*....|....*....|....*..
gi 568984531   696 ALVCPVCNLEQetsDLTLFNIHVDICL 722
Cdd:smart00734   1 LVQCPVCFREV---PENLINSHLDSCL 24
umuC PRK03609
translesion error-prone DNA polymerase V subunit UmuC;
39-313 7.08e-05

translesion error-prone DNA polymerase V subunit UmuC;


Pssm-ID: 179607 [Multi-domain]  Cd Length: 422  Bit Score: 45.91  E-value: 7.08e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531  39 HVDMDAFYAAVEMRDNPELKDKPIAVGSMS---MLATSNyHARRFGVRAAMPGFIAK--RLCPQLIIVPPNFDKYRAVSK 113
Cdd:PRK03609   4 LCDVNSFYASCETVFRPDLRGKPVVVLSNNdgcVIARSA-EAKALGIKMGDPWFKQKdlFRRCGVVCFSSNYELYADMSN 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 114 EVKEILAEYDPNFMAMSLDEAYLNITqhlqerqdwpedkrryfikmgnylKIDTPRqeanELTEYERsispllfedsppd 193
Cdd:PRK03609  83 RVMSTLEELSPRVEIYSIDEAFCDLT------------------------GVRNCR----DLTDFGR------------- 121

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 194 lqpqgspfqlnseeqnnpqiaqnsvvfgtsaeevvkEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKpngQYQilPSR 273
Cdd:PRK03609 122 ------------------------------------EIRATVLQRTHLTVGVGIAQTKTLAKLANHAAK---KWQ--RQT 160

                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
gi 568984531 274 SAVMD---------FIKDLPIRKVSGIGKVTEKMLMALGIVTCTELYQQ 313
Cdd:PRK03609 161 GGVVDlsnlerqrkLLSLQPVEEVWGVGRRISKKLNAMGIKTALDLADT 209
PolY_like cd03468
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ...
 231-427 2.74e-03

DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.


Pssm-ID: 176458 [Multi-domain]  Cd Length: 335  Bit Score: 40.83  E-value: 2.74e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 231 IRFRIEQKTT-LTASAGIAPNTMLAKVCSDKNKPNGQYQIlPSRSAVMDFIKDLPIRkVSGIGKVTEKMLMALGIVT--- 306
Cdd:cd03468  119 ASLRAALATLgLSARAGIADTPGAAWLLARAGGGRGVLRR-EALAAALVLLAPLPVA-ALRLPPETVELLARLGLRTlgd 196

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568984531 307 CTELYqqRALLSLLFSE--TSWHYFLhialgLGSTDLARDG----ERKSMSVERTF-SEISKTEEQysLCQELCAELAHD 379
Cdd:cd03468  197 LAALP--RAELARRFGLalLLRLDQA-----YGRDPEPLLFspppPAFDFRLELQLeEPIARGLLF--PLRRLLEQLCAF 267

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 568984531 380 LQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAIAKELLR 427
Cdd:cd03468  268 LALRGLGARRLSLTLFREDGRVTRVLVGLARPSRDDLPLLRLLRERLE 315
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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