LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 578824666  71 RLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQ 113
Cdd:cd12944    1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

Caulimovirus viroplasmin; This family consists of various caulimovirus viroplasmin proteins. The viroplasmin protein is encoded by gene VI and is the main component of viral inclusion bodies or viroplasms. Inclusions are the site of viral assembly, DNA synthesis and accumulation. Two domains exist within gene VI corresponding approximately to the 5' third and middle third of gene VI, these influence systemic infection in a light-dependent manner.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 578824666  199 YYGVcpvyedvpARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKF 249
Cdd:pfam01693   2 YYAV--------AKGRKPGIYTTWDECKAQVKGFPGAKYKSFKTREEAEAF 44

LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 578824666  71 RLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQ 113
Cdd:cd12944    1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

Caulimovirus viroplasmin; This family consists of various caulimovirus viroplasmin proteins. The viroplasmin protein is encoded by gene VI and is the main component of viral inclusion bodies or viroplasms. Inclusions are the site of viral assembly, DNA synthesis and accumulation. Two domains exist within gene VI corresponding approximately to the 5' third and middle third of gene VI, these influence systemic infection in a light-dependent manner.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 578824666  199 YYGVcpvyedvpARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKF 249
Cdd:pfam01693   2 YYAV--------AKGRKPGIYTTWDECKAQVKGFPGAKYKSFKTREEAEAF 44

Ankyrin repeats (3 copies);

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578824666  355 MHVAAKENQASICQLTLDVLENPDFMR------LMYpdddeAMLQKR---IRYVVDlYLNTPDKMGYDTPLHFACKFGNA 425
Cdd:pfam12796   1 LHLAAKNGNLELVKLLLENGADANLQDkngrtaLHL-----AAKNGHleiVKLLLE-HADVNLKDNGRTALHYAARSGHL 74

                  ....*....
gi 578824666  426 DVVNVLSSH 434
Cdd:pfam12796  75 EIVKLLLEK 83

LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 578824666  71 RLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQ 113
Cdd:cd12944    1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

LEM (Lap2/Emerin/Man1) domain found in lamina-associated polypeptide 2 (LAP2), LEM domain-containing protein 1 (LEMP-1) and similar proteins; This CD corresponds to the LEM domain of LAP2, LEMP-1 and similar proteins. LAP2, also termed thymopoietin (TP), or thymopoietin-related peptide (TPRP), is composed of isoform alpha and isoforms beta/gamma and may be involved in chromatin organization and post-mitotic reassembly. Some of LAP2 isoforms are inner nuclear membrane proteins that can bind to nuclear lamins and chromatin, while others are non-membrane nuclear polypeptides. All LAP2 isoforms contain an N-terminal LEM domain that is connected to a highly divergent LEM-like domain by an unstructured linker. Although LEM and LEM-like domains share the same structural fold composed of two large parallel alpha helices, the biochemical nature of the solvent-accessible residues is completely different, indicating that the two domains may target different protein surfaces. The LEM domain interacts with the nonspecific DNA binding protein barrier-to-autointegration factor (BAF) while the LEM-like domain is involved in chromosome binding. LEMP-1, also termed cancer/testis antigen 50 (CT50), is encoded by LEMD1, a novel testis-specific gene expressed in colorectal cancers. It may function as a cancer-testis antigen for immunotherapy of colorectal carcinoma (CRC). LEMP-1 contains an N-terminal LEM domain.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 578824666  72 LKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLaQALLEQ 113
Cdd:cd12940    2 VTELSDEELKAQLLKYGVKPGPITASTRKLYEKKL-QKLLDQ 42

Caulimovirus viroplasmin; This family consists of various caulimovirus viroplasmin proteins. The viroplasmin protein is encoded by gene VI and is the main component of viral inclusion bodies or viroplasms. Inclusions are the site of viral assembly, DNA synthesis and accumulation. Two domains exist within gene VI corresponding approximately to the 5' third and middle third of gene VI, these influence systemic infection in a light-dependent manner.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 578824666  199 YYGVcpvyedvpARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKF 249
Cdd:pfam01693   2 YYAV--------AKGRKPGIYTTWDECKAQVKGFPGAKYKSFKTREEAEAF 44

Ankyrin repeats (3 copies);

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578824666  355 MHVAAKENQASICQLTLDVLENPDFMR------LMYpdddeAMLQKR---IRYVVDlYLNTPDKMGYDTPLHFACKFGNA 425
Cdd:pfam12796   1 LHLAAKNGNLELVKLLLENGADANLQDkngrtaLHL-----AAKNGHleiVKLLLE-HADVNLKDNGRTALHYAARSGHL 74

                  ....*....
gi 578824666  426 DVVNVLSSH 434
Cdd:pfam12796  75 EIVKLLLEK 83

LEM (Lap2/Emerin/Man1) domain found in LEM domain-containing protein 2 (LEM2); This CD corresponds to the LEM domain that is responsible for the interaction with chromatin protein barrier-to-autointegration factor (BAF). LEM2, also termed LEMD2, is a novel Man1-related ubiquitously expressed inner nuclear membrane protein required for normal nuclear envelope morphology. Association with lamin A is required for its proper nuclear envelope localization. It also binds to lamin C and plays an important role in the organization of lamin A/C complexes. LEM2 contains an N-terminal LEM domain, two putative transmembrane domains and a MAN1-Src1p C-terminal (MSC) domain, but lacks the Man1-specific C-terminal RNA recognition motif (RRM).

                         10        20        30
                 ....*....|....*....|....*....|..
gi 578824666  75 LNPDDLREEIVKAGLKCGPITSTTRFIFEKKL 106
Cdd:cd12941    1 LTDAELRRELLALGFRPGPITETTRKVYIKKL 32