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Conserved domains on  [gi|1370483943|ref|XP_024309256|]
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histone-lysine N-methyltransferase SETD2 isoform X2 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SRI pfam08236
SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA ...
 2323-2411 1.20e-29

SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation. This domain is conserved from yeast to humans. Members of this family form a compact, closed three-helix bundle, with an up-down-up topology. The first and second helices are antiparallel to each other and are of similar length; the third helix, which is packed across helices alpha1 and alpha2 is slightly shorter, consisting of only 15 amino acids. Most conserved hydrophobic residues are largely buried in the interior of the structure and form an extensive and contiguous hydrophobic core that stabilizes the packing of the three-helix bundle. This domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.


:

Pssm-ID: 462404  Cd Length: 83  Bit Score: 113.75  E-value: 1.20e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 2323 AKKSKEVFRKEMSQFIVQCLNPYRKPdCKvgrittTEDFKHLARKLTHGVMNKELKYCKNPEDLEC-NENVKHKTKEYIK 2401
Cdd:pfam08236    1 EEKQEKKFEKTLAPHVVNVLNKYRKK-LG------KEDFKRLAKELTKILVAKELKKCPNRDPPEElSEKKKKKVKEFVK 73

                           90
                   ....*....|
gi 1370483943 2402 KYMQKFGAVY 2411
Cdd:pfam08236   74 DYMEKFGAKY 83
AWS smart00570
associated with SET domains; subdomain of PRESET
 1451-1505 3.50e-20

associated with SET domains; subdomain of PRESET


:

Pssm-ID: 197795  Cd Length: 50  Bit Score: 85.53  E-value: 3.50e-20
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|....*
gi 1370483943  1451 KRMQCECTPLSKDEraqgeIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQH 1505
Cdd:smart00570    1 DIMTCECKPTDDDE-----TACGSDCLNRMLFIECSSSCPCGSYCSNQRFQKRQY 50
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 2247-2276 9.01e-12

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


:

Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 61.37  E-value: 9.01e-12
                           10        20        30
                   ....*....|....*....|....*....|
gi 1370483943 2247 LPPNWKTARDPEGKIYYYHVITRQTQWDPP 2276
Cdd:pfam00397    1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
SET super family cl40432
SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain superfamily; The Su(var)3-9, ...
 1505-1530 4.98e-06

SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain superfamily; The Su(var)3-9, Enhancer-of-zeste, Trithorax (SET) domain superfamily corresponds to SET domain-containing lysine methyltransferases, which catalyze site and state-specific methylation of lysine residues in histones that are fundamental in epigenetic regulation of gene activation and silencing in eukaryotic organisms. SET domains appear to be protein-protein interaction domains. It has been demonstrated that SET domains mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). A subset of SET domains has been called PR domains. These domains are divergent in sequence from other SET domains, but also appear to mediate protein-protein interaction. The SET domain consists of two regions known as N-SET and C-SET. C-SET forms an unusual and conserved knot-like structure of probable functional importance. In addition to N-SET and C-SET, an insert region (I-SET) and flanking regions of high structural variability form part of the overall structure. Some family members contain a pre-SET domain, which is found in a number of histone methyltransferases (HMTase), and a post-SET domain, which harbors a zinc-binding site.


The actual alignment was detected with superfamily member cd19172:

Pssm-ID: 394802 [Multi-domain]  Cd Length: 142  Bit Score: 48.35  E-value: 4.98e-06
                           10        20
                   ....*....|....*....|....*.
gi 1370483943 1505 HADVEVILTEKKGWGLRAAKDLPSKE 1530
Cdd:cd19172      1 YAKVEVFRTEKKGWGLRAAEDLPKGT 26
PostSET smart00508
Cysteine-rich motif following a subset of SET domains;
1530-1546 6.56e-05

Cysteine-rich motif following a subset of SET domains;


:

Pssm-ID: 214703  Cd Length: 17  Bit Score: 41.62  E-value: 6.56e-05
                            10
                    ....*....|....*..
gi 1370483943  1530 EAQKCFCGSANCRGYLG 1546
Cdd:smart00508    1 KKQPCLCGAPNCRGFLG 17
 
Name Accession Description Interval E-value
SRI pfam08236
SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA ...
 2323-2411 1.20e-29

SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation. This domain is conserved from yeast to humans. Members of this family form a compact, closed three-helix bundle, with an up-down-up topology. The first and second helices are antiparallel to each other and are of similar length; the third helix, which is packed across helices alpha1 and alpha2 is slightly shorter, consisting of only 15 amino acids. Most conserved hydrophobic residues are largely buried in the interior of the structure and form an extensive and contiguous hydrophobic core that stabilizes the packing of the three-helix bundle. This domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.


Pssm-ID: 462404  Cd Length: 83  Bit Score: 113.75  E-value: 1.20e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 2323 AKKSKEVFRKEMSQFIVQCLNPYRKPdCKvgrittTEDFKHLARKLTHGVMNKELKYCKNPEDLEC-NENVKHKTKEYIK 2401
Cdd:pfam08236    1 EEKQEKKFEKTLAPHVVNVLNKYRKK-LG------KEDFKRLAKELTKILVAKELKKCPNRDPPEElSEKKKKKVKEFVK 73

                           90
                   ....*....|
gi 1370483943 2402 KYMQKFGAVY 2411
Cdd:pfam08236   74 DYMEKFGAKY 83
AWS smart00570
associated with SET domains; subdomain of PRESET
 1451-1505 3.50e-20

associated with SET domains; subdomain of PRESET


Pssm-ID: 197795  Cd Length: 50  Bit Score: 85.53  E-value: 3.50e-20
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|....*
gi 1370483943  1451 KRMQCECTPLSKDEraqgeIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQH 1505
Cdd:smart00570    1 DIMTCECKPTDDDE-----TACGSDCLNRMLFIECSSSCPCGSYCSNQRFQKRQY 50
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 2247-2276 9.01e-12

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 61.37  E-value: 9.01e-12
                           10        20        30
                   ....*....|....*....|....*....|
gi 1370483943 2247 LPPNWKTARDPEGKIYYYHVITRQTQWDPP 2276
Cdd:pfam00397    1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
AWS pfam17907
AWS domain; This entry represents the AWS (associated with SET domain) domain. This is a zinc ...
 1471-1503 5.02e-10

AWS domain; This entry represents the AWS (associated with SET domain) domain. This is a zinc binding domain. The full AWS domain contains 8 cysteines. This entry represents the N-terminal part of the domain, with the C-terminal part interwoven with the SET domain.


Pssm-ID: 465559  Cd Length: 39  Bit Score: 56.67  E-value: 5.02e-10
                           10        20        30
                   ....*....|....*....|....*....|....
gi 1370483943 1471 ACGEDCLNRLLMIECSSR-CPNGDYCSNRRFQRK 1503
Cdd:pfam17907    6 GCGSDCINRMLFVECTPKtCPCGESCQNQRFQRK 39
WW cd00201
Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; ...
 2248-2278 5.67e-10

Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; functions as an interaction module in a diverse set of signalling proteins; binds specific proline-rich sequences but at low affinities compared to other peptide recognition proteins such as antibodies and receptors; WW domains have a single groove formed by a conserved Trp and Tyr which recognizes a pair of residues of the sequence X-Pro; variable loops and neighboring domains confer specificity in this domain; there are five distinct groups based on binding: 1) PPXY motifs 2) the PPLP motif; 3) PGM motifs; 4) PSP or PTP motifs; 5) PR motifs.


Pssm-ID: 238122 [Multi-domain]  Cd Length: 31  Bit Score: 56.00  E-value: 5.67e-10
                           10        20        30
                   ....*....|....*....|....*....|.
gi 1370483943 2248 PPNWKTARDPEGKIYYYHVITRQTQWDPPTW 2278
Cdd:cd00201      1 PPGWEERWDPDGRVYYYNHNTKETQWEDPRE 31
WW smart00456
Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds ...
 2247-2278 6.98e-10

Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds proline-rich polypeptides.


Pssm-ID: 197736 [Multi-domain]  Cd Length: 33  Bit Score: 56.07  E-value: 6.98e-10
                            10        20        30
                    ....*....|....*....|....*....|..
gi 1370483943  2247 LPPNWKTARDPEGKIYYYHVITRQTQWDPPTW 2278
Cdd:smart00456    2 LPPGWEERKDPDGRPYYYNHETKETQWEKPRE 33
SET_SETDB-like cd10538
SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) ...
 1453-1527 4.09e-09

SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2, and similar proteins; The family includes SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2. SETDB1 (EC 2.1.1.43; also termed ERG-associated protein with SET domain (ESET), histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, or lysine N-methyltransferase 1E (KMT1E)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB2 (EC 2.1.1.43; also termed chronic lymphocytic leukemia deletion region gene 8 protein (CLLD8), or lysine N-methyltransferase 1F (KMT1F)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It is involved in left-right axis specification in early development and mitosis. SUV39H1 (also termed histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, lysine N-methyltransferase 1A, KMT1A, position-effect variegation 3-9 homolog, SUV39H, or Su(var)3-9 homolog 1) and SUV39H2 (also termed histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, lysine N-methyltransferase 1B, KMT1B, or Su(var)3-9 homolog 2), both act as histone-lysine N-methyltransferases that specifically trimethylate 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' as substrate. They mainly function in heterochromatin regions, thereby playing central roles in the establishment of constitutive heterochromatin at pericentric and telomere regions. EHMT1 (also termed Eu-HMTase1, G9a-like protein 1, GLP, GLP1, histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, lysine N-methyltransferase 1D, or KMT1D) and EHMT2 (also termed Eu-HMTase2, HLA-B-associated transcript 8, histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, lysine N-methyltransferase 1C, KMT1C, or protein G9a), both act as histone-lysine N-methyltransferases that specifically mono- and dimethylate 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. This family also includes the pre-SET domain, which is found in a number of histone methyltransferases (HMTase), N-terminal to the SET domain. Pre-SET domain is a zinc binding motif which contains 9 conserved cysteines that coordinate three zinc ions. It is thought that this region plays a structural role in stabilizing SET domains. Most family members, except for Arabidopsis thaliana SUVH9, contain a post-SET domain which harbors a zinc-binding site.


Pssm-ID: 380936 [Multi-domain]  Cd Length: 217  Bit Score: 58.92  E-value: 4.09e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370483943 1453 MQCECTPLSKDERAQGEIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10538     36 SKCACAAESDGIFAYTKNGLLRLNNSPPPIFECNSKCSCDDDCKNRVVQRGLQARLQVFRTSKKGWGVRSLEFIP 110
SET_SETD2 cd19172
SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and ...
 1505-1530 4.98e-06

SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and similar proteins; SETD2 (also termed HIF-1, huntingtin yeast partner B, huntingtin-interacting protein 1 (HIP-1), huntingtin-interacting protein B, lysine N-methyltransferase 3A or protein-lysine N-methyltransferase SETD2) acts as histone-lysine N-methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using demethylated 'Lys-36' (H3K36me2) as substrate. It has been shown that methylation is a posttranslational modification of dynamic microtubules and that SETD2 methylates alpha-tubulin at lysine 40, the same lysine that is marked by acetylation on microtubules. Methylation of microtubules occurs during mitosis and cytokinesis and can be ablated by SETD2 deletion, which causes mitotic spindle and cytokinesis defects, micronuclei, and polyploidy.


Pssm-ID: 380949 [Multi-domain]  Cd Length: 142  Bit Score: 48.35  E-value: 4.98e-06
                           10        20
                   ....*....|....*....|....*.
gi 1370483943 1505 HADVEVILTEKKGWGLRAAKDLPSKE 1530
Cdd:cd19172      1 YAKVEVFRTEKKGWGLRAAEDLPKGT 26
PostSET smart00508
Cysteine-rich motif following a subset of SET domains;
1530-1546 6.56e-05

Cysteine-rich motif following a subset of SET domains;


Pssm-ID: 214703  Cd Length: 17  Bit Score: 41.62  E-value: 6.56e-05
                            10
                    ....*....|....*..
gi 1370483943  1530 EAQKCFCGSANCRGYLG 1546
Cdd:smart00508    1 KKQPCLCGAPNCRGFLG 17
PRP40 COG5104
Splicing factor [RNA processing and modification];
2251-2292 8.17e-03

Splicing factor [RNA processing and modification];


Pssm-ID: 227435 [Multi-domain]  Cd Length: 590  Bit Score: 41.22  E-value: 8.17e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 1370483943 2251 WKTARDPEGKIYYYHVITRQTQWDPPTWESPGDDASLEHEAE 2292
Cdd:COG5104     58 WKECRTADGKVYYYNSITRESRWKIPPERKKVEPIAEQKHDE 99
 
Name Accession Description Interval E-value
SRI pfam08236
SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA ...
 2323-2411 1.20e-29

SRI (Set2 Rpb1 interacting) domain; The SRI (Set2 Rpb1 interacting) domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation. This domain is conserved from yeast to humans. Members of this family form a compact, closed three-helix bundle, with an up-down-up topology. The first and second helices are antiparallel to each other and are of similar length; the third helix, which is packed across helices alpha1 and alpha2 is slightly shorter, consisting of only 15 amino acids. Most conserved hydrophobic residues are largely buried in the interior of the structure and form an extensive and contiguous hydrophobic core that stabilizes the packing of the three-helix bundle. This domain mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.


Pssm-ID: 462404  Cd Length: 83  Bit Score: 113.75  E-value: 1.20e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 2323 AKKSKEVFRKEMSQFIVQCLNPYRKPdCKvgrittTEDFKHLARKLTHGVMNKELKYCKNPEDLEC-NENVKHKTKEYIK 2401
Cdd:pfam08236    1 EEKQEKKFEKTLAPHVVNVLNKYRKK-LG------KEDFKRLAKELTKILVAKELKKCPNRDPPEElSEKKKKKVKEFVK 73

                           90
                   ....*....|
gi 1370483943 2402 KYMQKFGAVY 2411
Cdd:pfam08236   74 DYMEKFGAKY 83
AWS smart00570
associated with SET domains; subdomain of PRESET
 1451-1505 3.50e-20

associated with SET domains; subdomain of PRESET


Pssm-ID: 197795  Cd Length: 50  Bit Score: 85.53  E-value: 3.50e-20
                            10        20        30        40        50
                    ....*....|....*....|....*....|....*....|....*....|....*
gi 1370483943  1451 KRMQCECTPLSKDEraqgeIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQH 1505
Cdd:smart00570    1 DIMTCECKPTDDDE-----TACGSDCLNRMLFIECSSSCPCGSYCSNQRFQKRQY 50
WW pfam00397
WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds ...
 2247-2276 9.01e-12

WW domain; The WW domain is a protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro.


Pssm-ID: 459800 [Multi-domain]  Cd Length: 30  Bit Score: 61.37  E-value: 9.01e-12
                           10        20        30
                   ....*....|....*....|....*....|
gi 1370483943 2247 LPPNWKTARDPEGKIYYYHVITRQTQWDPP 2276
Cdd:pfam00397    1 LPPGWEERWDPDGRVYYYNHETGETQWEKP 30
AWS pfam17907
AWS domain; This entry represents the AWS (associated with SET domain) domain. This is a zinc ...
 1471-1503 5.02e-10

AWS domain; This entry represents the AWS (associated with SET domain) domain. This is a zinc binding domain. The full AWS domain contains 8 cysteines. This entry represents the N-terminal part of the domain, with the C-terminal part interwoven with the SET domain.


Pssm-ID: 465559  Cd Length: 39  Bit Score: 56.67  E-value: 5.02e-10
                           10        20        30
                   ....*....|....*....|....*....|....
gi 1370483943 1471 ACGEDCLNRLLMIECSSR-CPNGDYCSNRRFQRK 1503
Cdd:pfam17907    6 GCGSDCINRMLFVECTPKtCPCGESCQNQRFQRK 39
WW cd00201
Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; ...
 2248-2278 5.67e-10

Two conserved tryptophans domain; also known as the WWP or rsp5 domain; around 40 amino acids; functions as an interaction module in a diverse set of signalling proteins; binds specific proline-rich sequences but at low affinities compared to other peptide recognition proteins such as antibodies and receptors; WW domains have a single groove formed by a conserved Trp and Tyr which recognizes a pair of residues of the sequence X-Pro; variable loops and neighboring domains confer specificity in this domain; there are five distinct groups based on binding: 1) PPXY motifs 2) the PPLP motif; 3) PGM motifs; 4) PSP or PTP motifs; 5) PR motifs.


Pssm-ID: 238122 [Multi-domain]  Cd Length: 31  Bit Score: 56.00  E-value: 5.67e-10
                           10        20        30
                   ....*....|....*....|....*....|.
gi 1370483943 2248 PPNWKTARDPEGKIYYYHVITRQTQWDPPTW 2278
Cdd:cd00201      1 PPGWEERWDPDGRVYYYNHNTKETQWEDPRE 31
WW smart00456
Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds ...
 2247-2278 6.98e-10

Domain with 2 conserved Trp (W) residues; Also known as the WWP or rsp5 domain. Binds proline-rich polypeptides.


Pssm-ID: 197736 [Multi-domain]  Cd Length: 33  Bit Score: 56.07  E-value: 6.98e-10
                            10        20        30
                    ....*....|....*....|....*....|..
gi 1370483943  2247 LPPNWKTARDPEGKIYYYHVITRQTQWDPPTW 2278
Cdd:smart00456    2 LPPGWEERKDPDGRPYYYNHETKETQWEKPRE 33
SET_SETDB-like cd10538
SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) ...
 1453-1527 4.09e-09

SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2, and similar proteins; The family includes SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2. SETDB1 (EC 2.1.1.43; also termed ERG-associated protein with SET domain (ESET), histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, or lysine N-methyltransferase 1E (KMT1E)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB2 (EC 2.1.1.43; also termed chronic lymphocytic leukemia deletion region gene 8 protein (CLLD8), or lysine N-methyltransferase 1F (KMT1F)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It is involved in left-right axis specification in early development and mitosis. SUV39H1 (also termed histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, lysine N-methyltransferase 1A, KMT1A, position-effect variegation 3-9 homolog, SUV39H, or Su(var)3-9 homolog 1) and SUV39H2 (also termed histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, lysine N-methyltransferase 1B, KMT1B, or Su(var)3-9 homolog 2), both act as histone-lysine N-methyltransferases that specifically trimethylate 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' as substrate. They mainly function in heterochromatin regions, thereby playing central roles in the establishment of constitutive heterochromatin at pericentric and telomere regions. EHMT1 (also termed Eu-HMTase1, G9a-like protein 1, GLP, GLP1, histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, lysine N-methyltransferase 1D, or KMT1D) and EHMT2 (also termed Eu-HMTase2, HLA-B-associated transcript 8, histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, lysine N-methyltransferase 1C, KMT1C, or protein G9a), both act as histone-lysine N-methyltransferases that specifically mono- and dimethylate 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. This family also includes the pre-SET domain, which is found in a number of histone methyltransferases (HMTase), N-terminal to the SET domain. Pre-SET domain is a zinc binding motif which contains 9 conserved cysteines that coordinate three zinc ions. It is thought that this region plays a structural role in stabilizing SET domains. Most family members, except for Arabidopsis thaliana SUVH9, contain a post-SET domain which harbors a zinc-binding site.


Pssm-ID: 380936 [Multi-domain]  Cd Length: 217  Bit Score: 58.92  E-value: 4.09e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1370483943 1453 MQCECTPLSKDERAQGEIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10538     36 SKCACAAESDGIFAYTKNGLLRLNNSPPPIFECNSKCSCDDDCKNRVVQRGLQARLQVFRTSKKGWGVRSLEFIP 110
SET_SETMAR cd10544
SET domain (including pre-SET and post-SET domains) found in SET domain and mariner ...
 1483-1527 8.77e-07

SET domain (including pre-SET and post-SET domains) found in SET domain and mariner transposase fusion protein (SETMAR) and similar proteins; SETMAR (also termed metnase) is a DNA-binding protein that is indirectly recruited to sites of DNA damage through protein-protein interactions. It has a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. SETMAR also acts as a histone-lysine N-methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3. It specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining.


Pssm-ID: 380942 [Multi-domain]  Cd Length: 254  Bit Score: 52.69  E-value: 8.77e-07
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*
gi 1370483943 1483 IECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10544     67 FECNSMCKCSESCQNRVVQNGLQFKLQVFKTPKKGWGLRTLEFIP 111
SET_SETDB1 cd10517
SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) ...
 1455-1527 3.02e-06

SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) and similar proteins; SETDB1 (EC 2.1.1.43; also termed ERG-associated protein with SET domain (ESET), histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, or lysine N-methyltransferase 1E (KMT1E)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.


Pssm-ID: 380915 [Multi-domain]  Cd Length: 288  Bit Score: 51.52  E-value: 3.02e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 1455 CECTPLSKDERA---QGEIACGEDCLNRLLM-------IECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAK 1524
Cdd:cd10517     68 CACQQLTIEATAatpGGQINPSAGYQYRRLMeklptgvYECNSRCKCDKRCYNRVVQNGLQVRLQVFKTEKKGWGIRCLD 147


                   ...
gi 1370483943 1525 DLP 1527
Cdd:cd10517    148 DIP 150
SET_SETD2 cd19172
SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and ...
 1505-1530 4.98e-06

SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and similar proteins; SETD2 (also termed HIF-1, huntingtin yeast partner B, huntingtin-interacting protein 1 (HIP-1), huntingtin-interacting protein B, lysine N-methyltransferase 3A or protein-lysine N-methyltransferase SETD2) acts as histone-lysine N-methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using demethylated 'Lys-36' (H3K36me2) as substrate. It has been shown that methylation is a posttranslational modification of dynamic microtubules and that SETD2 methylates alpha-tubulin at lysine 40, the same lysine that is marked by acetylation on microtubules. Methylation of microtubules occurs during mitosis and cytokinesis and can be ablated by SETD2 deletion, which causes mitotic spindle and cytokinesis defects, micronuclei, and polyploidy.


Pssm-ID: 380949 [Multi-domain]  Cd Length: 142  Bit Score: 48.35  E-value: 4.98e-06
                           10        20
                   ....*....|....*....|....*.
gi 1370483943 1505 HADVEVILTEKKGWGLRAAKDLPSKE 1530
Cdd:cd19172      1 YAKVEVFRTEKKGWGLRAAEDLPKGT 26
PostSET smart00508
Cysteine-rich motif following a subset of SET domains;
1530-1546 6.56e-05

Cysteine-rich motif following a subset of SET domains;


Pssm-ID: 214703  Cd Length: 17  Bit Score: 41.62  E-value: 6.56e-05
                            10
                    ....*....|....*..
gi 1370483943  1530 EAQKCFCGSANCRGYLG 1546
Cdd:smart00508    1 KKQPCLCGAPNCRGFLG 17
SET_SUV39H_Clr4-like cd20073
SET domain (including pre-SET and post-SET domains) found in of Schizosaccharomyces pombe H3K9 ...
 1454-1537 1.18e-04

SET domain (including pre-SET and post-SET domains) found in of Schizosaccharomyces pombe H3K9 methyltransferase Clr4, and similar proteins; This subfamily contains fission yeast Schizosaccharomyces pombe H3K9 methyltransferase Clr4 (also known as Suv39h), the sole homolog of the mammalian SUV39H1 and SUV39H2 enzymes, that has a critical role in preventing aberrant heterochromatin formation. It is known to di- and tri-methylate Lys-9 of histone H3, a central heterochromatic histone modification, with its specificity profile most similar to that of the human SUV39H2 homolog.


Pssm-ID: 380999 [Multi-domain]  Cd Length: 259  Bit Score: 46.41  E-value: 1.18e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 1454 QCEC------TPLSKDEraQGEIACGEDCLnrllMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd20073     41 SCQCledsneKSFAYDE--YGRVRANTGSI----IYECNENCDCGINCPNRVVQRGRKLPLEIFKTKHKGWGLRCPRFIK 114

                           90
                   ....*....|
gi 1370483943 1528 SKEAQKCFCG 1537
Cdd:cd20073    115 AGTFIGVYLG 124
SET_EHMT cd10543
SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine ...
 1481-1527 1.57e-04

SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase EHMT1, EHMT2 and similar proteins; This family includes EHMT1 (also termed Eu-HMTase1, G9a-like protein 1, GLP, GLP1, histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, lysine N-methyltransferase 1D, or KMT1D) and EHMT2 (also termed Eu-HMTase2, HLA-B-associated transcript 8, histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, lysine N-methyltransferase 1C, KMT1C, or protein G9a), both act as histone-lysine N-methyltransferases that specifically mono- and dimethylate 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin.


Pssm-ID: 380941 [Multi-domain]  Cd Length: 231  Bit Score: 45.41  E-value: 1.57e-04
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*..
gi 1370483943 1481 LMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10543     66 LIFECNRACSCWRNCRNRVVQNGIRYRLQLFRTRGMGWGVRALQDIP 112
SET_AtSUVH-like cd10545
SET domain found in Arabidopsis thaliana histone H3-K9 methyltransferases (SUVHs) and similar ...
 1457-1528 1.65e-04

SET domain found in Arabidopsis thaliana histone H3-K9 methyltransferases (SUVHs) and similar proteins; Arabidopsis thaliana SUVH protein (also termed suppressor of variegation 3-9 homolog protein) is a histone-lysine N-methyltransferase that methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression. Some family members contain a post-SET domain which binds a Zn2+ ion. Most family members, except for Arabidopsis thaliana SUVH9, contain a post-SET domain which harbors a zinc-binding site.


Pssm-ID: 380943 [Multi-domain]  Cd Length: 232  Bit Score: 45.47  E-value: 1.65e-04
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1370483943 1457 CTPLSKD----ERAQGEIACGED--CLNRLLMI-ECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLPS 1528
Cdd:cd10545     30 CTDGASDcacvKKNGGEIPYNFNgrLIRAKPAIyECGPLCKCPPSCYNRVTQKGLRYRLEVFKTAERGWGVRSWDSIPA 108
SET_SETDB cd10541
SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1), ...
 1426-1527 8.51e-04

SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1), SET domain bifurcated 2 (SETDB2), and similar proteins; SETDB1 (EC 2.1.1.43; also termed ERG-associated protein with SET domain (ESET), histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, or lysine N-methyltransferase 1E (KMT1E)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB2 (EC 2.1.1.43; also termed chronic lymphocytic leukemia deletion region gene 8 protein (CLLD8), or lysine N-methyltransferase 1F (KMT1F)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It is involved in left-right axis specification in early development and mitosis.


Pssm-ID: 380939 [Multi-domain]  Cd Length: 236  Bit Score: 43.30  E-value: 8.51e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 1426 PCYFDL-IEENVYLTERKKNKSHRDIKRMQC--------ECTPLSKD--------ERAQGEIACGedclnrllMIECSSR 1488
Cdd:cd10541      2 PFYYIPdISYGKFLVGCDCTDGCRDKSKCAChqltiqatACTPGGQDnptagyqyKRLEECLPTG--------VYECNKL 73

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|
gi 1370483943 1489 CP-NGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10541     74 CKcDPNMCQNRLVQHGLQVRLQLFKTQNKGWGIRCLDDIA 113
SET_SUV39H cd10542
SET domain (including pre-SET and post-SET domains) found in suppressor of variegation 3-9 ...
 1484-1527 1.05e-03

SET domain (including pre-SET and post-SET domains) found in suppressor of variegation 3-9 homologs, SUV39H1, SUV39H2 and similar proteins; This family includes SUV39H1 (also termed histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, lysine N-methyltransferase 1A, KMT1A, position-effect variegation 3-9 homolog, SUV39H, or Su(var)3-9 homolog 1) and SUV39H2 (also termed histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, lysine N-methyltransferase 1B, KMT1B, or Su(var)3-9 homolog 2), both act as histone-lysine N-methyltransferases that specifically trimethylate 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' as substrate. They mainly function in heterochromatin regions, thereby playing central roles in the establishment of constitutive heterochromatin at pericentric and telomere regions. Also included are Schizosaccharomyces pombe H3K9 methyltransferase Clr4 (SUV39H homolog) and Neurospora crassa DIM-5, both of which also methylate 'Lys-9' of histone H3.


Pssm-ID: 380940 [Multi-domain]  Cd Length: 245  Bit Score: 43.05  E-value: 1.05e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*
gi 1370483943 1484 ECSSRCPNGDYCSNRRFQRKQHADVEVILT-EKKGWGLRAAKDLP 1527
Cdd:cd10542     65 ECNSRCKCGPDCPNRVVQRGRKVPLCIFRTsNGRGWGVKTLEDIK 109
SET_EHMT2 cd10533
SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine ...
 1448-1527 3.79e-03

SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins; EHMT2 (also termed Eu-HMTase2, HLA-B-associated transcript 8, histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, lysine N-methyltransferase 1C (KMT1C), or protein G9a) acts as a histone-lysine N-methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin.


Pssm-ID: 380931 [Multi-domain]  Cd Length: 239  Bit Score: 41.54  E-value: 3.79e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370483943 1448 RDIKRMQ-CECTPLSKDER---AQGEIACGEDCLNRLL----------MIECSSRCPNGDYCSNRRFQRKQHADVEVILT 1513
Cdd:cd10533     19 RNITHLQhCTCVDDCSSSNclcGQLSIRCWYDKDGRLLqefnkiepplIFECNQACSCWRNCKNRVVQSGIKVRLQLYRT 98

                           90
                   ....*....|....
gi 1370483943 1514 EKKGWGLRAAKDLP 1527
Cdd:cd10533     99 AKMGWGVRALQTIP 112
SET_EHMT1 cd10535
SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine ...
 1481-1527 4.63e-03

SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 1 (EHMT1) and similar proteins; EHMT1 (also termed Eu-HMTase1, G9a-like protein 1, GLP, GLP1, histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, or lysine N-methyltransferase 1D (KMT1D)) acts as a histone-lysine N-methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin.


Pssm-ID: 380933 [Multi-domain]  Cd Length: 231  Bit Score: 41.07  E-value: 4.63e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*..
gi 1370483943 1481 LMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLP 1527
Cdd:cd10535     66 LIFECNHACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIP 112
PRP40 COG5104
Splicing factor [RNA processing and modification];
2251-2292 8.17e-03

Splicing factor [RNA processing and modification];


Pssm-ID: 227435 [Multi-domain]  Cd Length: 590  Bit Score: 41.22  E-value: 8.17e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 1370483943 2251 WKTARDPEGKIYYYHVITRQTQWDPPTWESPGDDASLEHEAE 2292
Cdd:COG5104     58 WKECRTADGKVYYYNSITRESRWKIPPERKKVEPIAEQKHDE 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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