Conserved Protein Domain Family
Glyco_hydro_81

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pfam03639: Glyco_hydro_81 
Glycosyl hydrolase family 81 N-terminal domain
Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein Swiss:Q9UVV0 two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the N-terminal beta sandwich domain.
Statistics
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PSSM-Id: 427418
Aligned: 90 rows
Threshold Bit Score: 170.118
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EPE09474      24 HPEE-PKG--INrhATPGPIQTNKFYANLLLGGQELPAYVIPYTVSW-NKGrgpthgwGLAVSYATPeqrvfgptdgacn 99 
XP_003531855  16 DPSTyFSSnlLS-----SPLPTNSFFQNFVIPNGSQAEYIHPYLIKT-SNS-------SLSASYPLL------------- 69 
KRH45046      17 DPSTyFSPnlLS-----SPLPTNSFFQNFVIPNGTQPEYIHPYLIQS-SNS-------SLSASFPLL------------- 70 
KRH18281      75 DPSKfFSSnlLS-----SPLPTNSFFQNFVLKNGDQQEYIHPYLIKS-SNS-------SLSLSYPSR------------- 128
XP_003555070  76 EPSNfFSSnlVS-----NPLPTNSFFQNFVLKNGDQPEYIHPYLIKS-SNS-------CLSLSYPSR------------- 129
XP_002282971  80 DPSPfFSPdlLT-----APLPTNSFFQNFVLKHGDQPEYIHPYLIKS-SSS-------SLSLSYPSQ------------- 133
Q9LFT3        67 DPSRfFSHdlLS-----SPLPTNSFFQNFTLNNGDQAEYFHPYIIKP-STS-------SLSISYPSL------------- 120 thale cress
XP_002305966  75 DPSLfLSPslLS-----SPLPTSSFFQNFTLKNGDQPEYIHPYLIKS-SLS-------SLSVSYPSQ------------- 128
XP_006338286  61 EPSDyFSSqlVS-----TPLPTNSFFQNFVLKNGDQPEYIQPYLIRS-ANS-------SISISYPSQ------------- 114
1_pfamImport   1 NPKL----------LADVPFPTGAWWTNLVLAEGKSAIASLPYVFKIlSEK--------LHVSYPFR------------- 49 
EPE09474     100 hpsrpasYYYSPPI-----IQNMVLDAAELmGSQTT----LTVEQPRDMSLMVNLHaapGL-PPTIRFPIVQGQGFVTGL 169
XP_003531855  70 -------ILFTTAVlyqtfVADITISSTQTts------qnHVISSYSDLGVTLDIP------SSNLRFFLSRGSPFLTVS 130
KRH45046      71 --------LFTTALlyqafVPDLTISASKT---YSSyqqnRVVSSYSDLGVTLDIP------SSNLRFFLVRGSPYITAS 133
KRH18281     129 --------QASSAVifqvfNPDLTISAPQG--PKQGppgkHLISSYSDLSVTLDFP------SSNLSFFLVRGSPYLTVS 192
XP_003555070 130 --------FMNPSFiyqvfNPDLTISSSQK--SHPsh-lnHTISSHNDLSVTLDIP------SSNLRFFLVRGSPFLTLS 192
XP_002282971 134 --------FLTSVFiyqifNPDLTISASNK--TDPDsqrrHIVSSFSDLSVTLDLP------SSNLRFFLVRGSPFLTCA 197
Q9LFT3       121 --------SHNSAFiyeafNADITITGSDG--PDPHsrksHLISSFSDLGVTLDFP------SSNLRFFLVRGSPFITFS 184 thale cress
XP_002305966 129 --------SHNSSFiyqvfVVDLTISATNK--TDPNpgksHVISSYSDLSVTLDMP------SSNFRFFLVRGSPFLTCL 192
XP_006338286 115 --------NISSDFieqifRPDLTISASKN----PKpnqrHVISSYSDLSVTLDLP------SSNLRFFLVRGSPYLTFA 176
1_pfamImport  50 --------VVTPTDivegfIAQMVFSSQP--SGLSSlpmaHHVVDFDDFSTTVRFS---QGqTEAFRVFLVRGSPYITME 116
EPE09474     170 YHGAMPVLRSATAIKSFahy-dmPTKtSSGS-WV--RRFALQLGDSSTWLVYARnlpRTPnLELvHdehNGAIVRMQRPF 245
XP_003531855 131 VTSPTS-----LSITTV------HTIvSLSSnDDnnTKYTLKLNNTQTWLIYTs---SPIyFTH-N---NASEVT-SKPF 191
KRH45046     134 VTKPTP-----LSIKTV------HTVvSLSS-DDynTKHTLKLNNSQAWIIYTs---SPIyLNH-----VPSEVT-SKPF 192
KRH18281     193 VTQPTP-----LSITTI------HSIlSFSS-NDsnTKYTFQFNNGQTWLLYAt---SPIkLNH-----TLSEIT-SNAF 251
XP_003555070 193 VTQPTP-----LSITTI------HAIlSFTS-SEslTKHTFHLNNGQTWFLYAs---SPIrLSH-----GLSEIT-SDAF 251
XP_002282971 198 VSGGTA-----LSITTI------HAIlSFSP-SSsrTKYTIKLNNGQTWLLYTs---SAInLTH-----DISSIT-SDAY 256
Q9LFT3       185 V--NSS-----ITISTI------HAVlSLSG-NTssTKYTVKLNNNQTWLIYAs---SPInLTK-D---GVSSINCGDGF 243 thale cress
XP_002305966 193 ITGNTE-----ITISTI------HAIlSFSS-SNslTKYTVKLNNNQTWLIYSs---SPInLSH-----DLSSIT-SEGF 251
XP_006338286 177 VNGNTS-----ISISTV------DPIlKLSC-DSsfTKYTIRLRNRQTWILYAs---APIhLTD-----NVSTIK-SGGF 235
1_pfamImport 117 FRHSRPLIEAMDGLQIVhfkrllGLKlMNGD-EVpfATFAAELNNGQTWYVYASd--STLeLQL-N---RNRQLTTDMEF 189
EPE09474     246 YGSVQVARminsgNPQEW--QAYD--------SAAGVFATGIQLKGkTSYEGkT--GNYTFVFEKGdvagePEf------ 307
XP_003531855 192 SGIIRVAVl-----pNHNyvTILD--------KFSTCYP---------LSGNaTlvEPFRVVYEWQk------------- 236
KRH45046     193 SGIIRIAAl---pDSDSKyvETLD--------KFSSCYP---------VSGDaAlkKPFSVEYKWQk------------- 239
KRH18281     252 SGIIRIALl---pDSDSKheAVLD--------KYSSCYP---------VSGKaVfrEPFCVEYNWEk------------- 298
XP_003555070 252 SGIIRIALl---pGSDWKheAVLD--------RFSSCYP---------VSGEaVfaRPFCVEYKWEk------------- 298
XP_002282971 257 SGIIRIAIl---pDSNPRfeSILD--------RFSSCYP---------ISGKaElsKPFCLEYRWDk------------- 303
Q9LFT3       244 SGIIRIVVl---pNPNPYfeTILD--------GFSCSYP---------VSGDaDftKPFALEYKWEk------------- 290 thale cress
XP_002305966 252 SGIIRIAVl---pDSDPKyeAILD--------RFSSCYP---------ISGDaSftKPFCLEYRWEk------------- 298
XP_006338286 236 SGVIRIALl---aDSDSQfeKILD--------KCSSTYP---------VSGSaIl-GSFALKYKWDv------------- 281
1_pfamImport 190 TGVLRVAL-------------CLDailwphllESASVYAVGGEVVH-SMDPQdPekALLDFQWKTR-----SFstfhgqt 250
EPE09474     308 ----mAR-TPLLMFALPHHYSAFG--------RTTQAAMQsYVALLTtTKGLARAVLADSWTMEEPELPADvgflpWNP 373
XP_003531855 237 -----EGsGDLLMLAHPLHVKLLSn------nYNGLVTVLnDFKYRS-IDGDLVGVVGDSWVLETNPIPVT-----WYS 298
KRH45046     240 -----KRsGDLLMLAHPLHAKLLS--------YDRDVTVLnDFKYRS-IDGDLVGVVGDSWVLETNPIPVT-----WNS 299
KRH18281     299 -----KDsGDLLLLAHPLHVQLLRn-------GDNDVKILeDLKYKS-IDGDLVGVVGDSWVLKTDPLFVT-----WHS 359
XP_003555070 299 -----RGwGDLLMLAHPLHIQLLAd------sGSEDVSVLsDFKYGS-IDGELVGVVGDSWLLKTDPVSVT-----WHS 360
XP_002282971 304 -----KGwGDLLMLAHPLHLRLLS--------ADDEVAVLdDFKFNS-IDGELVGVVGDSWKLKLDPVSIT-----WNS 363
Q9LFT3       291 -----RGyGDLLMLAHPLHLKLLSt-------NDCSITVLdNFKYNS-IDGDLVGVIGDSWVLKPDPVSVT-----WHS 351 thale cress
XP_002305966 299 -----KGwGDLLMLAHPLHLRLLSs-------KDSDVTVLdDFKYNS-IDGELVGVVGDSWVLKTDPVSVT-----WHS 359
XP_006338286 282 -----KGaGKLLMLAHPLHLRLLSk-------ADASVTVLeDVKYSS-MDGELVGVIGDTWDLEAESIPVS-----WHS 342
1_pfamImport 251 devtaEEnDKLLMLTLPHHLDVMQvqkgagtlKEGKNRLLpKLKYIC-IRGPMQGIFGAVWHMEETLTPVE-----WNY 323
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