2HF3,1D4X,1YAG


Conserved Protein Domain Family
ASKHA_NBD_actin_Arp-T1-3
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cd13397: ASKHA_NBD_actin_Arp-T1-3 
nucleotide-binding domain (NBD) of actin, actin-related proteins T1-T3 (Arp-T1-3), and similar proteins
The family includes actin and human actin-related proteins T1, T2, and T3. Actin is a ubiquitous protein involved in the formation of filaments that are major components of the cytoskeleton. It is a highly dynamic structural protein network involved in processes such as cell contraction, cell motility, vesicle trafficking, intracellular organization, cytokinesis, endocytosis and apoptosis. Arp-T1, encoded by ACTRT1/ARPT1 gene expressed in testis, negatively regulates the Hedgehog (SHH) signaling, binds to the promoter of the SHH signaling mediator, GLI1, and inhibits its expression. Arp-T2 (also called actin-related protein M2; encoded by ACTRT2/ARPM2 gene expressed in testis and various other cell types) and Arp-T3 (also called actin-related protein M1; encoded by ACTRT3/ARPM1 gene expressed in all tested human tissues) play general roles in the organization of the cytoskeleton like other cytoplasmic actin-related proteins.
Links
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Statistics
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PSSM-Id: 466848
Aligned: 31 rows
Threshold Bit Score: 538.311
Created: 1-Nov-2012
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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nucleotide
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Structure:2HF3; Drosophila melanogaster actin binds CaADP, contacts at 4A
  • Structure:1D4X; Caenorhabditis elegans actin binds MgATP, contacts at 4A
  • Structure:1YAG; Saccharomyces cerevisiae actin binds MgATP, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                #### #                                                         #         
2HF3_A          5 AALVVDNGSGMCKAGFAGdDAPRAVFPSIVGrprhqgv--mvgmgqKDSYVGDEAQSKRgiLTLKYPIEHGIVTNWDDME 82   fruit fly
Q9BYD9          6 LPVVIDNGSGMIKAGVAGcREPQFIYPNIIGrakgqs---raaqggLELCVGDQAQDWRssLFISYPVERGLITSWEDME 82   human
Q8TDY3         10 PAVIFDNGSGFCKAGLSGeFGPRHMVSSIVGhlkfqap--saeanqKKYFVGEEALYKQeaLQLHSPFERGLITGWDDVE 87   human
XP_005986089   10 PAVIFDNGSGLCKAGIAGdNAPRSVITSIVGrskakat--mlgagqKEYYIGEEAQAKRgvLSLKYPIEHGIVTSWDDME 87   coelacanth
ETE61273      420 KSVIIDNGSGLCKSGISGeISPRHVMATVLGyprsklp--lskakqKECYVGFEAQHRQdvLSLTYPIEAGVITSWDEME 497  king cobra
RXM90967       10 PAVIFDNGSGLCKCGVAGdSVPRSVITSIVGhskakakvttlgaghNEYYVGDEAQAKRglLSLKCPVEQGFVTCWDDME 89   sterlet
NP_001006111   10 PAVIFDIGSGNCKAGMSGeDLPTAVVPSVVGhlsdrsa--mlgagqQPYYVGEMAMSKRaiLDLVFPIKEGIVKSWDNMI 87   tropical claw...
XP_007900278   17 PAVIIDNGSGLCKAGIAGdNAPRSVITSIVGrpkakat--mlgagqKEYYIGEQAQAKRgiLTLKYPIKHGIVASWDDME 94   elephant shark
EFC43396        6 RAICMDIGFGNIRAGFVCrDAPRAVFPPYIGrpkikg---miipgmKNVFVGDEAVIKKaiLKLSKPMDRDVITNWDDME 82   Naegleria gru...
EKG04871       10 PAVVLDNGSGNIKCGFAGeEIPRCVFPSVTGvsmnars--sgssssQRVYVGDEALQEKg-LRYFYPMEHGIVYDWDQME 86   Trypanosoma c...

Feature 1                                                              #                # ####    
2HF3_A         83 KIWHHTFYNELrvAPEEHPVLLTEApLNPKANREKMTQIMFETFnTPAMYVAiQAVLSLYASGrTTGIVLDSGDGVSHTV 162  fruit fly
Q9BYD9         83 IMWKHIYDYNLklKPCDGPVLITEPaLNPLANRQQITEMFFEHLgVPAFYMSiQAVLALFAAGfTTGLVLNSGAGVTQSV 162  human
Q8TDY3         88 RLWKHLFEWELgvKPSDQPLLATEPsLNPRENREKMAEVMFENFgVPAFYLSdQAVLALYASAcVTGLVVDSGDAVTCTV 167  human
XP_005986089   88 KVWRHVYECELrmKTNERPVLLTEApLNPLHNREKMTQIMFENFkVPAMYVAvQAVLALYASGrTTGMVMDSGDGVTHTV 167  coelacanth
ETE61273      498 TIWKYIYNKGLgvKAFERPLLMSESpLNPKEDRAKLTQLMFEYFkVPAFYLSiQAILSLYASArYTGIVIDCGFGVSHAV 577  king cobra
RXM90967       90 KIWRHLYECKLkmKPSERPVHLTEApLNPLTNREMMTEVMFENFkVPAMYVSvQALLALYASGrTTGLVIDSGDGITHTV 169  sterlet
NP_001006111   88 KIWKYLYKYELkkPSSEHPVFLTEApLNPLRNRHKMAEIFFENFnVPAMYVShQANLALYASGlTTGIVLDVGAGITHTV 167  tropical claw...
XP_007900278   95 KIWRHVYECELrlKSTERPVLLTEApLNPLQNRETMTEIMFETFkVPAMYVAvQAVLALYSSGrTTGLVLDSGDGVTHTV 174  elephant shark
EFC43396       83 ILFEHVFFNELrvEPENQGIILGEGcFNPKQNRRKLAEMLFEKFkVPLMNSCpNALLVLISSGrSSGVVLDCGEGTSSSV 162  Naegleria gru...
EKG04871       87 RVWRHAYEQLRv-PPERQAVLLTEApMNPISNREKMAETLFESFgVPALHVQiQAVLTLYSSGrTDGLVLDSGDGVTHLV 165  Trypanosoma c...

Feature 1                           ##  #                        #  ##                            
2HF3_A        163 PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGysft-tteEREIVRDIKEKLCYVALDfeqemata-----assssle 236  fruit fly
Q9BYD9        163 PIFEGYCLPHGVQQLDLAGLDLTNYLMVLMKNHGimll-sasDRKIVEDIKESFCYVAMNyeeemak-------kpdcle 234  human
Q8TDY3        168 PIFEGYSLPHAVTKLHVAGRDITELLMQLLLASGhtfp-cqlDKGLVDDIKKKLCYVALEpekelsr-------rpeevl 239  human
XP_005986089  168 PIYEGYCLPHAVSRLDVAGRDITEYFMRLLLESGhsfv-staEKEIVKDIKEKLCYVSLDpnhdmkk-------kveeiq 239  coelacanth
ETE61273      578 PVYEGYCLPHAVSQLGLGGRDITQFLRELLLENRqyfwnfpeANTVVEDVKVKLCFVALDahhresk-------pseelp 650  king cobra
RXM90967      170 PIYDGYCLPHATFRLGLAGRDITEYLMKLLQESGhsfg-scaKQEVVKDIKEKLCYVAVDltmelkk-------kpkenq 241  sterlet
NP_001006111  168 AIYDGVALPHAVSKLPVAGRTITQYLMKLLTENGynfi-taaEREIVRDIKEKLCYIALDpsaeyer-------npkdit 239  tropical claw...
XP_007900278  175 PIYEGYCLPHAVLRLDLAGRDITLYLVRILMENGhsfl-ttaEREIVKDIKEKLCYVAIDpylemrr-------rledvq 246  elephant shark
EFC43396      163 PIYEGCIIRNGVRRMDLAGNELTDHMDKLLKDSGnt----frDRSLVTDIKEKTCRVALDfereln---------eecsg 229  Naegleria gru...
EKG04871      166 PVFEGQTMPQTVRRLELAGRDLTEWMMELLSDELdrpfttsaDREVARRVKESLCYIPLFfeeelqaaeedginedakgk 245  Trypanosoma c...

Feature 1                                                                         ### ##          
2HF3_A        237 KSYELKDGQVITIGNERFRCPEALFQPsflgmeaCGIHETTYNSIMKC-DVDIRKDLYaNTVLSGGTTMYPGIADRMQKE 315  fruit fly
Q9BYD9        235 KVYQLPDGKVIQLHDQLFSCPEALFSPchmnleaPGIDKICFSSIMKC-DTGLRNSFFsNIILAGGSTSFPGLDKRLVKD 313  human
Q8TDY3        240 REYKLPDGNIISLGDPLHQAPEALFVPqqlgsqsPGLSNMVSSSITKC-DTDIQKILFgEIVLSGGTTLFHGLDDRLLKE 318  human
XP_005986089  240 KEYKLPDGNVIRIGSQLFRAPETLFLPanigveaPGVHKLTFTSIVKC-DIDVRRNLYsNVLLSGGSTLFPGLDERMLKE 318  coelacanth
ETE61273      651 KEYKLPDGNRIKIGDPLYLAPEILFTPsninhkgQGLHKLVAHSIKKC-DPTIRNMLYsNVLISGGSSLFPGLDERMFKG 729  king cobra
RXM90967      242 REYRLPDGNVIKIGDQLFRAPEALFVPasvqveaPGVHKMTFNSILKC-DSDVRRNLFsNMLMCGGSTLFPGLEERMLKE 320  sterlet
NP_001006111  240 KEYTLPDGNVIKIDSQLFRAPEALFSPsnigleaPGVQGLINNIIQKC-PIDIRRALLsNVVLSGGSTLFPGFDERVLRE 318  tropical claw...
XP_007900278  247 KEYKLPDGNVIKIHSQLFRAPEILFTPaslgyegPGVHRLAFKSILRC-DIDVRKYLYsNMLLSGGSTLFQGLDERLLKE 325  elephant shark
EFC43396      230 KNYELPDGNNLCIRSECIKCPEIMFQPsmigmdlDGVHKMVIDSINSNtDAKMKNKFFeNIILSGGSTMFDGFSERILKE 309  Naegleria gru...
EKG04871      246 EPFTLPDGEVIHVGRARFCCPEILFNPalaekpyDGIQHAVINCVNSC-PIDLRRQLLgSIVLSGGNTMFKGMQQRLQSE 324  Trypanosoma c...

Feature 1                              #                              
2HF3_A        316 ITALAps--tMKIKIIAPPERKYSVWIGGSILASLstFQQMWISKQEYDESG 365  fruit fly
Q9BYD9        314 IAKVApa--nTAVQVIAPPERKISVWMGGSILASLsaFQDMWITAAEFKEVG 363  human
Q8TDY3        319 LEQLAsk--dTPIKITAPPDRWFSTWIGASIVTSLssFKQMWVTAADFKEFG 368  human
XP_005986089  319 LQLQVps--gVPVKVIAPPERKYSVWIGASILTCLtsFKQMWITFSDYKEFG 368  coelacanth
ETE61273      730 LETEApq--gVPIKVIAPPDRWFSAWIGASIITSLstFKQMWVMAADYREYG 779  king cobra
RXM90967      321 IRVQIps--eVSVKVTAPPERKYTAWIGASFITCLtaFKQMWITASDYKEFG 370  sterlet
NP_001006111  319 LEKKApd--gVQIKVLAATDRKYSVWIGASVLSSMdsFKENWIRKSEYDEFG 368  tropical clawed frog
XP_007900278  326 IQSLApa--aIPVKVISPPERKYSVWIGASILTCLtsFKEMWITSNDYNEVG 375  elephant shark
EFC43396      310 LNQLAf---dNKVKVVAPPERKYSAWIGANILGSLstFESTWIRSEEYAEYG 358  Naegleria gruberi strain NEG-M
EKG04871      325 LAALAnkraaEDVRVVAASERKFSVWIGAAILASLtsFASEWITRTEYAEQG 376  Trypanosoma cruzi

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