1G8J


Conserved Protein Domain Family
Rieske_ArOX_small

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cd03476: Rieske_ArOX_small 
Click on image for an interactive view with Cn3D
Small subunit of Arsenite oxidase (ArOX) family, Rieske domain; ArOX is a molybdenum/iron protein involved in the detoxification of arsenic, oxidizing it to arsenate. It consists of two subunits, a large subunit similar to members of the DMSO reductase family of molybdenum enzymes and a small subunit with a Rieske-type [2Fe-2S] cluster. The large subunit of ArOX contains the molybdenum site at which the oxidation of arsenite occurs. The small subunit contains a domain homologous to the Rieske domains of the cytochrome bc(1) and cytochrome b6f complexes as well as naphthalene 1,2-dioxygenase. The Rieske domain is a [2Fe-2S] cluster binding domain involved in electron transfer.
Statistics
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PSSM-Id: 239558
Aligned: 9 rows
Threshold Bit Score: 207.623
Created: 20-Feb-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
[2Fe-2S]subunit
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:[2Fe-2S] cluster binding site [ion binding site]
Evidence:
  • Structure:1G8J; Alcaligenes faecalis Arsenite Oxidase binds [2Fe-2S] cluster; contacts at 3.5A
  • Comment:The Rieske [2Fe-2S] cluster is liganded to two histidine and two cysteine residues present in conserved sequences called Rieske motifs.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                # ##                   
1G8J_D        6 YPATAVSVAknl-aanEPSSFTYPDt--sSPCVAVKLGAPVPGGVGPDDDIVAYSVLCTHMGCPTSYds-----sSKTFS 77  Alcaligenes faec...
BAD71924     37 YPAVKVANLsqv-ktgEPIFFNYPDa--sSPAVLVKLGRPAIGGVGRERDIVAFSALCTHMGCPVQYe-------EGRFI 106 Thermus thermoph...
AAN05580     46 YPITAVGKAkgl-kvdAPVSFNYPDa--sSPCVAIKMGQPTPGGVGPNNDIVAHSILCTHMGCPVSYda-----sAKTFK 117 Cenibacterium ar...
ZP_00766194  89 YPRIKVATLndl--qsGAFGASYPDq--fSPIQILKIGKKVPGGVGPDGDIVAYSGLCTHMGCPMTYdp-----aTKIFV 159 Chloroflexus aur...
EAM42934     36 YPSVKIGNSktl-qpnQPVTFNFPDe--qSPCLLVKTGSEAIDGIGSGKDIVAFSTLCTHMGCPTTYs-------KGRLV 105 Chlorobium limic...
ZP_01064986  57 YPRKKIAKLsql-snhIPVHFQYPDegknSIAMLVKSDIECGGGIGLQRDIVAYSMTCTHQGGPLTGaykatgehRIVGQ 135 Vibrio sp. MED222
BAA81580     81 YPKVKIANVsel-regQPVFKEYIG----HRIVVIKLGEEAVNGVGPDKDIVAFSMLCTHMGGFLIFdg-----nTKTLI 150 Aeropyrum pernix K1
AAR05655     49 YPANRLANIsel-tlnEPLDVAYPDe--dAAGVLLKLGTRVEGGVGPDGDIVGFSTICPHKGFPLSYsa-----dNKTFN 120 arsenite-oxidisi...
BAB67501     79 YVKQKVANIsqltspgQYVTTMYMG----YLVYIIKTGVPSENGVGPNNDIVGFSASCAHMGYILVYdp-----sNNCLL 149 Sulfolobus tokod...
Feature 1       #  # #                                                
1G8J_D       78 CPCHFTEFDAEKAGQMICGEATADLPRVLLRYdaasDALTAVGVDGLIYGRQAN 131 Alcaligenes faecalis
BAD71924    107 CRCHYSMFDPAKAGQPYQGLASSWLPQIPLRIdg-kGDIYAVAVAGLIWGRVKN 159 Thermus thermophilus HB8
AAN05580    118 CPCHFSVFDPDNHGQMVCGQATENLPQIQLSYnaanDTFTAIGVTGLIYGRQSN 171 Cenibacterium arsenoxidans
ZP_00766194 160 CPCHYSHFDAAADAMLISGPATQHLPRIVLEVe--gQDIYAVGAQGLIYGRPHN 211 Chloroflexus aurantiacus J-10-fl
EAM42934    106 CKCHYSMFDVSRNGQSYQGLASQWIPQIVLREdpasGDIFAEGVQGLIYGRIGN 159 Chlorobium limicola DSM 245
ZP_01064986 136 CPFHLSTFDLRRHGIMVSGQAFESLPQVLLELe--gDDIYAVGVMGLIFGRMEN 187 Vibrio sp. MED222
BAA81580    151 CPLHFSQFDPSRGGQPVVGHATEYLPQVILEYdeasGDIFAVGFTALVYGRYDN 204 Aeropyrum pernix K1
AAR05655    121 CPGHFSVFDPEKGGQQVWGQATQNLPQYVLRVad-nGDIFAEGVDELIYGRLSN 173 arsenite-oxidising bacterium NT-26
BAB67501    150 CPQHFSQYDATRGGMQVVGHPNQFLPQLILEYeestGDIYALGFNRLVYGTYNT 203 Sulfolobus tokodaii str. 7

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