2WQT,6J57


Conserved Protein Domain Family
FAA_hydrolase

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pfam01557: FAA_hydrolase 
Fumarylacetoacetate (FAA) hydrolase family
This family consists of fumarylacetoacetate (FAA) hydrolase, or fumarylacetoacetate hydrolase (FAH) and it also includes HHDD isomerase/OPET decarboxylase from E. coli strain W. FAA is the last enzyme in the tyrosine catabolic pathway, it hydrolyses fumarylacetoacetate into fumarate and acetoacetate which then join the citric acid cycle. Mutations in FAA cause type I tyrosinemia in humans this is an inherited disorder mainly affecting the liver leading to liver cirrhosis, hepatocellular carcinoma, renal tubular damages and neurologic crises amongst other symptoms. The enzymatic defect causes the toxic accumulation of phenylalanine/tyrosine catabolites. The E. coli W enzyme HHDD isomerase/OPET decarboxylase contains two copies of this domain and functions in fourth and fifth steps of the homoprotocatechuate pathway; here it decarboxylates OPET to HHDD and isomerizes this to OHED. The final products of this pathway are pyruvic acid and succinic semialdehyde. This family also includes various hydratases and 4-oxalocrotonate decarboxylases which are involved in the bacterial meta-cleavage pathways for degradation of aromatic compounds. 2-hydroxypentadienoic acid hydratase encoded by mhpD in E. coli is involved in the phenylpropionic acid pathway of E. coli and catalyzes the conversion of 2-hydroxy pentadienoate to 4-hydroxy-2-keto-pentanoate and uses a Mn2+ co-factor. OHED hydratase encoded by hpcG in E. coli is involved in the homoprotocatechuic acid (HPC) catabolism. XylI in P. putida is a 4-Oxalocrotonate decarboxylase.
Statistics
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PSSM-Id: 460252
Aligned: 42 rows
Threshold Bit Score: 103.519
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2WQT_Q         58 VGRKVGLTHPKVQQQlg-------vDQPDfgtl---fadmC-YGDNE--IIPFSrvLQPRI------------------- 105 Escherichia co...
jgi:M446_2715  83 AAAWPGHVRLLRQAEga-------eAPSDsgtplrhaasdGfLAPRAtlPA------DPAAsl----------------- 132
Q4J6Y6        102 VRDFLAFRGHVEASYkrrg---qqiPEEWfki------piYyKGDPAafYGHMEnvPWPSYsna---------------- 156 Sulfolobus aci...
Q162G6        124 FTDFYAGRHHAFNVGslfrdpanalPPNWlhi------piGyNGRASsvVVSGTdiTRPNGqlkapdaemp--vfeaskr 195 Roseobacter de...
CAK38680      113 FSDFYCSFEHTRNV------------PSWfvi------psVyNGRQSslRISGTpiRRPWGvtldsnkpndppafrpsar 174
WP_000674021   80 IRDFYAFEQHVKTARgrrg---ldvVPEWydi------pvFyFTNHRavIGPNDvvIGPKKsek---------------- 134 Bacillus
jgi:Daci_0212  56 AGSQQGPGRVPGYWKsga----prrDAPLthap---lpeeGvWASPSvaGG--AplHLRGI------------------- 107
Q2UHQ1        133 FTDYTCSLEHVTNVGrvag--fgdiPPSFrnq------plAyNGRASsvVPSGTpiQRPYGvgadgly--------pcqk 196 Aspergillus or...
Q9CKS8         21 FVIALNSTQLLQRCQaq------feQAPYqalp---ktpvIyVLPEAthNRSGAnvSLPAGkkq---------------- 75  Pasteurella mu...
Q63MG5         21 YGALLNDRGALAALGda------vhAPPYhkpp---rasvLyVKPANthAGDGAavVVPAGvda---------------- 75  Burkholderia p...
2WQT_Q        106 ---EAEIALVLNR--DLPATd---iTFDELYNAIEWVLPALEVVgSRIRDWSIQ--Fv--DTVADNAScgVYVIGGP--- 170 Escherichia co...
jgi:M446_2715 133 -dvSAGLAVITGD--VAQGT-----APAQAAGAVRLVTLVTEVI-RRDRLKPDGl-Dl--DGPLAAGF--AASLGPV--- 195
Q4J6Y6        157 ldfELEIGAIVYR--KGVNI-----EKRKARDYILGYTIFNDFS-ARDIQMREMtcLlg----pAKGKdfANGLGPW--- 221 Sulfolobus aci...
Q162G6        196 fdlELEMGAIVGK--PSKGRv----SVQEADDMIFGYVLLNDWS-ARDIQAWEYq-Pl--GPFQAKAT--ATTISPW--- 260 Roseobacter de...
CAK38680      175 fdfELEMGVYLSHplPPGEIl----NINNAKDHIFGLVILNDWS-ARDIQTYEMa-Pl--GPFHSKGS--GTSISPW--- 241
WP_000674021  135 idyELEIACVIGK--EGRNI-----SREQAEEYIFGYCIMNDWS-ARDLQATEMkvGl--GPAKGKDF--ATSLGAH--- 199 Bacillus
jgi:Daci_0212 108 ---EAEIALRLGR--DVTAEmaaalQPGEGLELIDGITAAIEVVdTRWRQHLKAp-Pl--LLAADGLChgALVLGPWqda 179
Q2UHQ1        197 ldyEVELGMFISTkiPIGEHi----PASRARDYIFGFVLLNDWS-ARDVQFAEMi-Pl--GPFNGKSC--GTSISTW--- 263 Aspergillus or...
Q9CKS8         76 lrmEPHLAVVFGR--DTSRVc-----vEQAMHYVSGYLPVALYSlPHD--------SyyrPDIEGRCQegFCVLGQE--- 137 Pasteurella mu...
Q63MG5         76 leiGATLGVVFAR--RTTRV-----PAEQAFDFVKGFTLASDVS-I---PHPDYy----rPAVRFKCRdgFCPLGPA--- 137 Burkholderia p...
2WQT_Q        171 --AQR-PAGld-------------------------lkNCAMKMTR-------------------------NNEE--VSS 195 Escherichia co...
jgi:M446_2715 196 --AVTpDELgpdw----------------------qdgMVQRPLCVt-----------------------rNGAL--LGR 226
Q4J6Y6        222 --IVTkDEVge-------------------------ikGLRAYASV-------------------------DGEKwcDTK 249 Sulfolobus aci...
Q162G6        261 --IVTkAALepfrastppreke---llpylaetgpmlyDIDLEVGLape--------------------gkSETI--LSR 313 Roseobacter de...
CAK38680      242 --IVPiEALegsqcnarvqdpaplkhlawkgkedeatfDIELKARVird---------------------gksYT--VTE 296
WP_000674021  200 --LVTkEELdvyrn--------------------gdryELEMTAHV-------------------------NGKL--LSK 230 Bacillus
jgi:Daci_0212 180 srLLPrESPrd-------------------------wsAQVCRLRV-------------------------GAAP--LQA 207
Q2UHQ1        264 --VVTmDALeeagaivpakadvategkftsnsflkcaeDVSIDVSTfissmyfpcrsswemllmwdiggesHRTP--ISR 339 Aspergillus or...
Q9CKS8        138 --V-LkSAVqn-------------------------paELTINITV-------------------------NGSV--KKS 162 Pasteurella mu...
Q63MG5        138 --IVArASIgd-------------------------adALAVTVRI-------------------------DGER--RYE 163 Burkholderia p...
2WQT_Q        196 GRG-SECLGHPLNAAVW-LARKMaslgePLRTGDIILTGAL--------------------------------------- 234 Escherichia co...
jgi:M446_2715 227 PEAgSDLGSGFGELIAE-AARLR-----PLGAGTIVSAGPVsnrgidggpgrsvaegga----gfaslaearaaeivasg 296
Q4J6Y6        250 A---EDMQWSFEDMISY-VSQDE-----YIRPGDIFGSGTIsgc----------------------------------cg 286 Sulfolobus aci...
Q162G6        314 TNY-NVMYYSAAQQLTHhTTSGC-----PMRVGDLLGSGTIsgpekenrgsllel-------------swagkepltldt 374 Roseobacter de...
CAK38680      297 TNL-KELYWTPYQQVTHlCSAGE-----GLSTGDIFGTGTItsdvreeigvacll------------erklernilseas 358
WP_000674021  231 GNF-QDIYYTFAEMIER-ASEDV-----TLYPGDVIGSGTVgtgc--------------------------------ile 271 Bacillus
jgi:Daci_0212 208 FTG-THPLGDPLWLLAGwLQELVrr-ygSVPAGTVVTTGTW--------------------------------------- 246
Q2UHQ1        340 SNL-KHTYWSPFQMIAHhTSAGC-----GLAPGDLIGTGTLsssakqasedleqastglgrlgciheltaggknpvqlvn 413 Aspergillus or...
Q9CKS8        163 YVH-LAMKHSIPELISF-LSQFI-----AFKAGDILLTGTE--------------------------------------- 196 Pasteurella mu...
Q63MG5        164 AST-ANLIRPVARLIAD-VSAFM-----SFDAGDVLLVGVG--------------------------------------- 197 Burkholderia p...
2WQT_Q        235 -GPMVAVNAGDRFeahIe-------------gIGSVAATF 260 Escherichia coli K-12
jgi:M446_2715 297 wARLPYLAPGDRLrveVkdrg-------ghsvFGAIEHEV 329
Q4J6Y6        287 lDNGRLLRPGSLVelyIe-------------kIGILVNRV 313 Sulfolobus acidocaldarius
Q162G6        375 gETRTFLEDGDTLtlrGaakgd-----gyrigFGDCTGTL 409 Roseobacter denitrificans OCh 114
CAK38680      359 kDGLDFLADRDEV---ImegwcvnrltggkfgFGECRSVI 395
WP_000674021  272 lGTEEWLQDGDVVeltIt-------------gLGTLRNTV 298 Bacillus
jgi:Daci_0212 247 -NGLAFAQAGDLVvaeLd---------------GIAEARL 270
Q2UHQ1        414 dLSLVWLQDNDEI---Alegwage--gnsrigFGQVVGRV 448 Aspergillus oryzae
Q9CKS8        197 -DMPLLVGAGDQVsveFa-------------qLGCLTNTV 222 Pasteurella multocida
Q63MG5        198 -PHAPRAAAGSTIeidAp-------------gIGTLRHTL 223 Burkholderia pseudomallei
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