3M5K,3E10


Conserved Protein Domain Family
nitroreductase

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cd02150: nitroreductase 
nitroreductase family protein
A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.
Statistics
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PSSM-Id: 380325
Aligned: 28 rows
Threshold Bit Score: 185.882
Created: 24-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
FMN bindinghomodimer
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:3M5K: A Clostridioides difficile 630 nitroreductase homodimer binds two molecules of FMN, contacts at 4A
  • Citation:PMID 9654450

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           ### #                       ### #                       #  #                 
3M5K_A        12 ILNRKSVRKYKD-RPVEKEKIDKLIRAGXAAPSSRDRRPWEFIIVtdrkaLDTXAEGLPFARXLKETRQAIVVCGdtiks 90  Parabacteroides...
YP_100477     42 IFARKSVRAYLD-KEVEKEKIDWMLRAGMAAPSGKDIRPWEFVLVtdrvaLDSMAAALPYAKMLTQARYAIVVCGdvaqs 120 Bacteroides fra...
YP_003316790   8 ILSRRSIRRFRP-GEVGEEVISLLLQCACAAPSAANSRPWHFIVVtdrqvLDRMADAHPYGKMLFQAQLAIVVCGdptkn 86  Thermanaerovibr...
SDY11374      12 ILDRRSIRKFQKdRKVEPEKLEIILECAQAAPTAGGTRPWHFVVVderslLDALAEAHPYGKMLHEAGFAVVVCGdpsis 91  Acetomicrobium ...
OGV70128       5 IKSRRSVRQWKS-DAVREDDVEKVLDAAMNAPSAGNEQPWQFVVVtdkavLNKAAGLNPYGGFVKDAPVAILVCGdvrle 83  Lentisphaerae b...
TFH66553      23 IFSRKSVRTFKS-EPVPKEKLELLIRAGMAAPTAVDKRPWEFIVVtdravLKQLADDLPFAKMADKAVAAIIVGGdvrkq 101 Gemmatimonadale...
KPK31984       7 IRTRKSIRKYLS-KNIDSKIIEEILSAAMSAPSAMNEQPWQFIVIdkrdiLSKIPDFCPYSQMAKDAPLAILVCGddkge 85  Chlamydiae bact...
KKQ93975       8 ILNRKSINIFQD-KPIAKEDLDLLLKAAMAAPTGSDLRPWRFIVVtdkkmLIKISEALKSTEKIKGNSAAILICGdssks 86  candidate divis...
KUK93520       6 ILRRRSIRRFEE-KPVEHEKIKLILKAAMAAPSAMNRRPWEFIVVtdeakLSRIRKSLLFGRYKAPLAIVVCGDTsgltl 84  Thermotogales b...
CDB40631       7 LRTRRSVRQYIPnRDIPADDIRDILETAMLAPSGRNKQPWEFIVVndkskFPEITQAQPYCRFLEEASLAVIVRGnteee 86  Azospirillum sp...
Feature 1                  ##                   ####                                       # #   
3M5K_A        91 -----snawfLDCSAASQNLLLAAEsXGLGAVWTAVypypdriEIVRKELRLPdhixPLNVIPVGYPXqke-tpKNKYNV 164 Parabacteroides...
YP_100477    121 ------sywyMDCSAAAQNILLAAEaQGLGAVWTAAypyedriRVVRKYTELPgnivPLCVIPFGYPAtaq-epKQKFDE 193 Bacteroides fra...
YP_003316790  87 dy--aaryweEDCSAAMQNILLGATaLGLGSVWLGVkhspereAAMRMILSVPdhiaIMGIAAIGHPDeek-epHRGIDE 163 Thermanaerovibr...
SDY11374      92 df--akryweEDCSAAMQNILLASQaLGLGSVWLGVnnspgrpEAIRKVLGIPqhinVLGIAAIGYPAesk-epHSGYDN 168 Acetomicrobium ...
OGV70128      84 k---fpgywvQDCSAAVENMLLAAHaLGLGAVWTSVypiddriVGFRKLFNLPseviPFALIPLGYPRvkgrdaSSRFNP 160 Lentisphaerae b...
TFH66553     102 wggmdsdywiMDCSAATENILLAAEsMGLGAVWTAVypedsriRTVRQILGIPphivPLNLIPLGVPAgre-kpKDKYNP 180 Gemmatimonadale...
KPK31984      86 r----egfwiQDCSAATENLLLAAHeKSLGAVWTGVyprqgrvLEFQKMLKIPkniiPFALIVLGYPDekp-kqKDNFKR 160 Chlamydiae bact...
KKQ93975      87 -----erfwtFDCSLAAENIMIAAQsIGIGSHWISIrpnkertDNIKEIFNFPdsikPLCAIALGYSDqdt-vpKDKFDY 160 candidate divis...
KUK93520      85 kk--lkdlwvQDCSAATENLLIAAAnIGLGAVWLAVypfkgavKRISSIVGLDskiiPLCVVYVGYPAeek-eaRTQYDE 161 Thermotogales b...
CDB40631      87 m---apgawmVDCAAATENLLLACHaKKLGSCWCGIypdkermENFIRLFNLPphikPLSLVVIGYPAaeprqpEDRFKQ 163 Azospirillum sp...
Feature 1         
3M5K_A       165 Q 165 Parabacteroides distasonis ATCC 8503
YP_100477    194 K 194 Bacteroides fragilis YCH46
YP_003316790 164 G 164 Thermanaerovibrio acidaminovorans
SDY11374     169 Q 169 Acetomicrobium thermoterrenum DSM 13490
OGV70128     161 S 161 Lentisphaerae bacterium RIFOXYA12_FULL_48_11
TFH66553     181 A 181 Gemmatimonadales bacterium
KPK31984     161 E 161 Chlamydiae bacterium SM23_39
KKQ93975     161 A 161 candidate division CPR2 bacterium GW2011_GWC2_39_10
KUK93520     162 K 162 Thermotogales bacterium 46_20
CDB40631     164 Q 164 Azospirillum sp. CAG:260

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