Conserved Protein Domain Family
TrwB_AAD_bind

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pfam10412: TrwB_AAD_bind 
Type IV secretion-system coupling protein DNA-binding domain
The plasmid conjugative coupling protein TrwB forms hexamers from six structurally very similar protomers. This hexamer contains a central channel running from the cytosolic pole (made up by the AADs) to the membrane pole ending at the transmembrane pore shaped by 12 transmembrane helices, rendering an overall mushroom-like structure. The TrwB_AAD (all-alpha domain) domain appears to be the DNA-binding domain of the structure. TrwB, a basic integral inner-membrane nucleoside-triphosphate-binding protein, is the structural prototype for the type IV secretion system coupling proteins, a family of proteins essential for macromolecular transport between cells and export.
Statistics
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PSSM-Id: 431268
Aligned: 14 rows
Threshold Bit Score: 429.409
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q3SI88        148 LRIHPKLpPISKSRETRHILIVGGVGSGKTQTLLPLMRAARERGDRAIIFDFKGDFTPDFPSctarhkgraiEVE-DVIF 226 Thiobacillus d...
jgi:Ajs_4194  170 PYEIAGV-PMRVKSETLHTLFAGAQGTGKSQQFFALMKQVRARGKRMIVYDPTGEFTQAFYR----------EGK-DILM 237
CAM79179      180 KICFGGL-PLVKNSERLHILITGTTGTGKTNMLNELLPQIRSQQDRAIIIDLTGAFTDRFFD----------SKC-DKLL 247
Q6MB84        168 PISIGSL-PLIKGTETQHIMITGGTGSGKTNCLHHLLKSVRQQKQRAIIVDTTGLLTERYYR----------AGK-DILL 235 Candidatus Pro...
Q3HKI7        184 RYTIAGI-PFPPNAVEAQTGIFGTVGVGKTNAIKELLTTIRAQNGRAIIYDRMGGLVRDFYD----------PAT-DIII 251 Rhodobacter sp...
WP_011950430  224 PYTLAGI-PFPHRTEQSHAMLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYD----------PTR-DTIL 291 Sphingomonadaceae
jgi:Saro_3982 225 VYNIAGV-SFPWRSEQAHTIMIGSTGTGKTTQMRDMIAQMRVRQDRAVVFDLTGAYVEAFYN----------PET-DTIL 292
Q1GQ75        169 ALAIATV-PMPPRLETRHMAMVGTTGSGKTTVLRQILDGVEARGEAALVYDTSGEFVAQYYD----------PRRgDIIL 237 Sphingopyxis a...
jgi:Swit_0177 189 GLTLAGV-PLSREDETMHVAAIGATGSGKSTALHGLMAGALRRGDRHIVADPEGAAMSAFFA----------DG--DILL 255
Q3SHV9        122 RLEIGGV-PIPIYVEDGGFLLTGSPGTGKSQATTKMLDTLQAGGHRAVIADAEGIFYSRYAG----------DG--AVLF 188 Thiobacillus d...
Q3SI88        227 APWDSRSTLWAVARDVDTLSAAREFAARMIVEpgGGSGSPMWANAARQVLVACLVELQSTKPGDWgFADLVAGLTRPVDQ 306 Thiobacillus d...
jgi:Ajs_4194  238 NPLDSRSPNWNIWNEIAKDYHFDNMANGLIPD--PAESDPFWALAGRMVLKDVIAVLGREGRRTN-RDLYNAIAKSNLDA 314
CAM79179      248 NPLEKNSEQWLPWNDCFEAADFHDIASSFSNY--THRIDDFFAKNAELVLSEALKLYQDD--KDI-IKLIHTIIYSDNRQ 322
Q6MB84        236 NPLDSRGAPWHPWIECIDKTSYATMAESFIPQs-LSDSDNYWRTSARIVLGSLFEQLKDIQKNST---LADKILRDPLQE 311 Candidatus Pro...
Q3HKI7        252 NPFDARSHIWSPFGEASTPEALAQIAEVMIPQr-PGQNDPFWSQTARLVFEYSARSLLRAKRPTN-ADLRRAIMTISAEE 329 Rhodobacter sp...
WP_011950430  292 NPMDARCPAWSIFNDCRTHSEFTAAAAALIPSd-GGSSEPFWALAARTLFIEMCIRLMERGQTSN-LALSENLMTADLKR 369 Sphingomonadaceae
jgi:Saro_3982 293 NPMDERCPSWSLFDEGKNYADFTAIASAILPTd-GGGSDPFWMLGARTLFVQTCVQLMKLGQATN-AALAYRLMMADLEE 370
Q1GQ75        238 NPFDARCAIWTPFAEIAHPADADRIAQQLITEt-GQHDRDVWLETSRILVANMIRALWREGKCTL-PDLLHALQVRSKDD 315 Sphingopyxis a...
jgi:Swit_0177 256 DPFDARCARWDLLAEIESPSDYAFLASSLLPHl-GTGDHDQWISYAQRLLADTMENFVTLELGST-SDYVALLGSARIAE 333
Q3SHV9        189 NPLDRRSVSWSPLAELKSPQDCSALAKSLIPD--GHGAEASWNDAAQTVLEGLLRRCWET-SKTN-AELYRLACVADIEE 264 Thiobacillus d...
Q3SI88        307 LTEAARRYFPEAVKAlgDGQQNvTTAGIQINLMSYLAVIFDLArawPGPGIFSIREWLTEVGarrVK---TVILQHDGRF 383 Thiobacillus d...
jgi:Ajs_4194  315 MHALLQGTAGATYVD--PTTER-TGMSLKMTVQNQLEAFRFLP---DEGESFSIRKWVHEEG---DS---WMFITAREAM 382
CAM79179      323 FVKAFRNTAVSGIIS--ESAPE-TSAGIQSTLGKNITSLQYLK---PGGN-FSIKEWFSNSAe--TG---WLFITATPSQ 390
Q6MB84        312 LCDFVQGTKAASLID--MNSEK-TAASIRAVMSNFLTCFELLA---NTDQPFSIREWIQQEDp-iDN---WLFISCKTNE 381 Candidatus Pro...
Q3HKI7        330 LEKLLAGTPGAHFFG--KHVEK-TSASIRANMIAELRFLEFLR---DDGELFSAREWVKSER---PG---FVFLTGDAEH 397 Rhodobacter sp...
WP_011950430  370 VHRFLANTIADPLTA--PEAAR-MAESIRAVFNTNAQVLRFLP---DEGESFSIRDWITRDKk-pGS---ILFVTSNYVD 439 Sphingomonadaceae
jgi:Saro_3982 371 VHELLRNTIAEPLTA--PVAAR-MAESVRAVLNTNAQALLFIP---EGKEPFSICDWIRHQDk-pGS---ILFITSSHNE 440
Q1GQ75        316 LKLWLGNSSSARTFA--DDADR-ATGSVLFMLAKAANLIQFLRideGKETAFSFRDFLSGLDm-hPGpkpWIFVPRKEDY 391 Sphingopyxis a...
jgi:Swit_0177 334 LKELCAGTSSARYFE--EGGER-MLASILGTLAPAVAHLRLIAm--ASGEVFSLRRWIREG----RG---SLWMPYAANQ 401
Q3SHV9        265 LQQLLAGTAAMTVVG----NAR-YFGEVRPGLGRYLRCFEHLDpaaGRAS-FSVRDFITEE----TG---WLFISYTQEQ 331 Thiobacillus d...
Q3SI88        384 ETLARAFNSAVLGLAGQLIKSnlVRD-----SKERK-LWLFLDEFPELGKVETVLSLAAVGRSKGIRVVLGLQDISQVKK 457 Thiobacillus d...
jgi:Ajs_4194  383 REALKPVLSLWIDTAIKAVLD--LEP-----IHRER-LWFCIDELPTLQKLDILKLALTNTRKYGLCMVLGVQDFSQLYE 454
CAM79179      391 RATLQPLISAWISIAIKALMC--RNP-----NHDNKnIWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEA 463
Q6MB84        382 RAALNPLLSCWLSVAVRSLLA--LPP-----NFERR-LWYIIDELPTLNRLRDLDMLLAESRKYGGCAVLALQSPAQLEA 453 Candidatus Pro...
Q3HKI7        398 SAATRNIISTIIEVSANALMT--CEE-----SRDPK-VWFFLDEVPSLNRLPFLQSKLAEIRQFGGAFVLGYQVFAQLED 469 Rhodobacter sp...
WP_011950430  440 LPMNRALLTLWMDLAINRLMT--LPR-----TRSLR-TWFMFDELGALHRLPAIENGLQTARAFGGAMILGIHSFEKLVE 511 Sphingomonadaceae
jgi:Saro_3982 441 LVLNRALLSLWMNLAVHTLMR--LPR-----TRSLR-TWFFFDEVHALHRLPAIEDGLQTARGFGGAFVLGIHSFAKLAE 512
Q1GQ75        392 FEASKPLLACWLECAASALLG--HSP-----SAERR-LWFILDELADLPRVDNLARLLPEGRKFGAAVILTFQAIGQMRH 463 Sphingopyxis a...
jgi:Swit_0177 402 VAAMRGLVSCWMNIAILETLS--LPA-----SRDRR-IWFHIDELDALGRIEGLKDAQARLRKVGGCVAIGFQSFAQVKH 473
Q3SHV9        332 RAALLPLIQCTLDVASRAILS--LPHttgdrSKLRR-TWLVLDELPLLGRIMSLIPLLTNGSKHGAAVIATAQTIALFRE 408 Thiobacillus d...
Q3SI88        458 IYSEHDTNSIVSMVGTQVIAQ-VS--PGETAKYIAENLiGDRDLERLGVSIT--SGAGRAPGIFTSGGNRTSKWedkrel 532 Thiobacillus d...
jgi:Ajs_4194  455 IYGHDLAKTIISGCQTKLLLR-VT--DGAAAKLLAELM-GQAEVDEKDETLS--YGLSSQRDGVSVMARRQMRD------ 522
CAM79179      464 IYGSAECASMLDLFNSKFIFR-VS--DQVTAYKSALTL-GEEEIIETQENLS--YGSNTMRDGVNMNNVERKKL------ 531
Q6MB84        454 IYGQAVAQTLIGNCATKIVFA-EQ--NPLHAGRLADMF-GEQEIREYQKGLS--YGANDIRDGVSLNQQTRYQQ------ 521 Candidatus Pro...
Q3HKI7        470 IYGEKGAQAIAGNLNNRIVFN-TP--DARTAKLFSESL-GFEDVVEQRENLS--FGAHESRDGVAFMSQRTERP------ 537 Rhodobacter sp...
WP_011950430  512 VYGEQGARNLASLARSKLILA-TA--DLDTAEQCARYI-GNREVRQMDEAYS--YGYNNTRDASTLTPRKQVEP------ 579 Sphingomonadaceae
jgi:Saro_3982 513 TYGKEGAQNLASLARTKLILA-AA--DRDTAEHCSDYI-GHREVRMMDEAYS--YGYSNIRDAATITPRSEVQP------ 580
Q1GQ75        464 RYGQDLSESMLGCCNTKLFLQ-MT--DSESRQWASDTI-GSCEVELPTMTDA--LADGDYKPRTTLGRQRKVRP------ 531 Sphingopyxis a...
jgi:Swit_0177 474 VYGE-AAHTIVENCGNLLILRcGGggDESTANLANELI-GEREAERDDLSRSqsRGRNASQS-TSVQSRRSVDP------ 544
Q3SHV9        409 AYGHDATQTILATLGTWLTLR-VS--DAETAEYMSKSL-GDEEVRRVVAS----GGKNDSGKSDNWSEQHSTRR------ 474 Thiobacillus d...
Q3SI88        533 VVLPSELLQLgpdLDLGGVKVWMSGIgeDVLEVLIP 568 Thiobacillus denitrificans ATCC 25259
jgi:Ajs_4194  523 LVLTSEILTL---PDMTGYLLVPGDY--PIARVKYG 553
CAM79179      532 LVMPSEIMNL---PDLTCYVKLVGNF--PITKLTMK 562
Q6MB84        522 LITKTDIQFL---SRNQAFVRLPDNC--PIVRLNVP 552 Candidatus Protochlamydia amoebophila UWE25
Q3HKI7        538 IVTASEIQAL---PQFEALIRFAYDA--PTARVRFE 568 Rhodobacter sphaeroides 2.4.1
WP_011950430  580 LVIADDITNL---PSMHGFVKFPDGF--PAARILLE 610 Sphingomonadaceae
jgi:Saro_3982 581 LVIPDDIMRL---PSLRGFLVFPEGF--DAARIRLT 611
Q1GQ75        532 AVLESEL-RL---PRHRGYLLFPDGL--PVARIALT 561 Sphingopyxis alaskensis
jgi:Swit_0177 545 AVLASEIMQL---PNREGFVKRALDP--RWQRVRFP 575
Q3SHV9        475 VVMPSQLQNL---PRLHGYLNISGPL--PVCAVTLP 505 Thiobacillus denitrificans ATCC 25259
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