Conserved Protein Domain Family
XLF

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pfam09302: XLF 
XLF-Cernunnos, XRcc4-like factor, NHEJ component
XLF (also called Cernunnos) is Xrcc4-like-factor, and interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated down-regulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. This family contains Nej1 (non-homologous end-joining factor), and Lif1, ligase-interacting factor. XLF forms one of the components of the NHEJ machinery for DNA non-homologous end-joining.
Statistics
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PSSM-Id: 462749
Aligned: 76 rows
Threshold Bit Score: 68.0615
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EHA55072       8 SWRPLPV-SHHA-VPNLLVSAAFStsPV-SYSV-YITDLANVWLERLDRRAILLRSLE---EKTSIDPSDDP-----ENF 75 
Q9P7J2         2 SWVQLRGhR------FHFIQASFDaeSNeAVQViHITDLVRVWSCTCSRNQIVNQAES---ERCPIDPF--------QTF 64  Schizosaccharom...
Q6NV18         9 PWVPVNIsGS-----DLLAKAWFG--DS-QYRV-LLTDLSTVWEEEMSTDDIQSRAQD---LNKRLR----------APA 66  zebrafish
XP_023185746  16 PWLPVCIdGC-----QLLAKSWFS--DA-EYCV-LLTDMQGVWEERMDTAAIERRAQE---LNRRLR----------AAV 73 
XP_019207773  16 PWLPVSIsGC-----QLLAKSWFG--ET-AYHI-LLTDMHCVWEERMDTADIQSRSQE---LNRRLR----------APV 73 
1_pfamImport   1 PWLPVSIgGC-----QLLAKSWFG--ET-AYHV-LLTDVSCVWEERMDSSAIQRRAKE---LNRRLQ----------AEV 58 
CCE85273       7 TWFSTSLsSQGTeKVKLLYGYEFSgeSK-SYLI-CTTDLNDVWIERLNKSQIIKKAKRfgiEDLS------------DKV 72 
Q6BKD1         7 SWNSIFIpFVNP--RKVIYGYQCDfnNK-AYSI-YITDFKNVWLERLSKQDIIDKANSygiEDTED-----------EKL 71  Debaryomyces ha...
XP_011276195   6 KWNVLNIdSPK----KYYYGVDFSteKI-HYSL-YITDFSNVWISDVDEEKLHFKAIQngiETESIN----------GVY 69 
CCC69827      15 EWHSCTInGQ-----PFLFSLNLSdnEE-STLFfINADQHPTFKACISSNDIKAQCTIqgfPSDSVTKITKEtykqiHQL 88 
EHA55072      76 KELLHRIGSAF-------DAENPHha--daSLALTSgletptgGV--N------DLILEV------TCILPAGLQPLKWP 132
Q9P7J2        65 ETLVDVLAEVLvnvs-------------------------sesRIqlRgsndegSLKIFC------TSKIAKDI-FLEWN 112 Schizosaccharom...
Q6NV18        67 QAFFSHLCSVArpcfsglDEDQISaaqaalEQH-GE------------------SLTVKL------KSELAGL--PFYWE 119 zebrafish
XP_023185746  74 QAFFSHLREAAep---wlSGGGGGeaqmcvWRR-DG------------------VVTLKL------KSELAGL--PFHWE 123
XP_019207773  74 KAFFTHLCEVVkpclagvGSQTENevqislTRQEDG------------------SINMRL------KSELAGL--PFYWE 127
1_pfamImport  59 EAFFSHLCDVVrpcltgsDGPPDGdahislARQPDG------------------NMRIKL------KSKLEEL--PFHWE 112
CCE85273      73 EDLLAVFSEGFgsr--------------skNKTLKF-------EIndQslgatdVINIIF-------------AQDIQWR 118
Q6BKD1        72 HYLLEVLRNGVecg--------------dvDKTLLFsi----tKInrTmsdnenTIQAKF-------------TGEIDWD 120 Debaryomyces ha...
XP_011276195  70 HTLISTFENNF-----------------------NSedvetrvDMneMkidkgiPLELRL------NLVKVYSNAELNWN 120
CCC69827      89 RSF-KKIEDGS-------------------------------------------RLQLTIvlsgdiTLNLTTIILGLSMQ 124
EHA55072     133 IKLKHGGASA---VATELVLPLVQ---AQMARQLELEALLAVIKEKDGVIS-------KVLDKLDGMGARLD------QI 193
Q9P7J2       113 WTLWLTPPET---IAKMVWFPLINhhlFESETDTSPGSLMSIIRDQKSLLNdktwfkeALNERSSSSNPSTPrk---rSA 186 Schizosaccharom...
Q6NV18       120 FRCTTTPVAV---VCRQLVRPLLA---MTLVLQRQAEDLAALLARKDAEI-----------QDYQENGAVLSrarlqtEP 182 zebrafish
XP_023185746 124 FRCSPAPVAL---VCSQLVRPLLA---MSRLLHRQLEEQGELLARKDQEI-----------QDYRENGATLSrerlqtDV 186
XP_019207773 128 FHCTPAPVAL---VCIQLVRPLLT---MSRLLQKQVEQLGGLLVRKDAEI-----------QDYRENGATLSrerlqtDV 190
1_pfamImport 113 FHCTPAPVPV---VCGQLVRPLLA---MSHLLQRQVEQLGGLLGRKDAEI-----------QDYRENGATLSrgetqlKA 175
CCE85273     119 FELQKQSHQIateFFASMNIQQFR---NHAFLDYKIQQLERAIKDKDHYIL-------YLEQNYKAVNGN--------EI 180
Q6BKD1       121 FSVVKQSPSNaidVISQINFQQFE---NHNYLNYKVEQLERLINIKDHYIV-------YLSENYKAINGD--------EL 182 Debaryomyces ha...
XP_011276195 121 FDLVILPDSSaitILRNLIFHLST---IIFSLNEYKNDLIKVVEHKDSAIR-------FLGESIEGMNGG--------HL 182
CCC69827     125 EQLT---------LSENLIRQTLR---IIHIQGLYSNHLLNVITEKDSVIQ-------FLSDSLNDLGAS--------KI 177
EHA55072     194 F 194
Q9P7J2       187 F 187 Schizosaccharomyces pombe 972h-
Q6NV18       183 F 183 zebrafish
XP_023185746 187 F 187
XP_019207773 191 F 191
1_pfamImport 176 F 176
CCE85273     181 I 181
Q6BKD1       183 I 183 Debaryomyces hansenii
XP_011276195 183 V 183
CCC69827     178 I 178
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