2Y7J


Conserved Protein Domain Family
STKc_PhKG2

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cd14181: STKc_PhKG2 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine Kinase, Phosphorylase kinase Gamma 2 subunit
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Phosphorylase kinase (PhK) catalyzes the phosphorylation of inactive phosphorylase b to form the active phosphorylase a. It coordinates hormonal, metabolic, and neuronal signals to initiate the breakdown of glycogen stores, which enables the maintenance of blood-glucose homeostasis during fasting, and is also used as a source of energy for muscle contraction. PhK is one of the largest and most complex protein kinases, composed of a heterotetramer containing four molecules each of four subunit types: one catalytic (gamma) and three regulatory (alpha, beta, and delta). The gamma 2 subunit (PhKG2) is also referred to as the testis/liver gamma isoform. Mutations in its gene cause autosomal-recessive glycogenosis of the liver. The gamma subunit, when isolated, is constitutively active and does not require phosphorylation of the A-loop for activity. The regulatory subunits restrain this kinase activity until signals are received to relieve this inhibition. For example, the kinase is activated in response to hormonal stimulation, after autophosphorylation or phosphorylation by cAMP-dependent kinase of the alpha and beta subunits. The high-affinity binding of ADP to the beta subunit also stimulates kinase activity, whereas calcium relieves inhibition by binding to the delta (calmodulin) subunit. The PhKG2 subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 271083
Aligned: 4 rows
Threshold Bit Score: 601.96
Created: 11-Dec-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:includes ATP binding and substrate binding sites
  • Comment:based on the structure of Oryctolagus cuniculus phosphorylase kinase gamma 1 subunit bound with Mg2-ATP and peptide substrate, defined at 4A contact distance
  • Citation:PMID 9362479

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         ## ## # #            # #                                     # 
2Y7J_A        85 DWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRaTGHEFAVKIMEVTAERlsPEQLEEVREATRRETHILRQVAgHPHIIT 164 human
AAH88919      13 DWAGAKEFYQKYDPKEIIGRGVSSTVRRCIHReTGRQFAVKIIEVTPERlsPEQLKEVRLSTAKEMEILHHVSnHPSIIS 92  African clawed ...
XP_003227745  13 DWAGAKEFYQKYDPKDVIGRGVSSVVRRCIHKqTGQEYAVKIIEVTPERmtLQQLEEVRSSTSKEIAILRQVSgHPFIIT 92  green anole
NP_957256     13 DWVGAKEFYQKYDPKEVIGRGVSSVVRRCVHKhTGQEFAVKIIEITAEKmtEQQLEEVKSSTLKEIHVLNLVKgHPSIIT 92  zebrafish
Feature 1                      ## #   # #                                   ## # ## #          # 
2Y7J_A       165 LIDSYESsSFMFLVFDLMRKGELFDYLTEKVaLSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDnMQIRLSDF 244 human
AAH88919      93 LIDSYESsTFIFLVFDLMKRGELFDYLTEKVtLSEKETRCIMRSLLEAVYYLHTNNIVHRDLKPENILMDDcLNIKLSDF 172 African clawed ...
XP_003227745  93 LIDSYETsTFMFLVFDLMRRGELFDYLTEKVtLSEKETRCIMRALLEAVSYLHANHIIHRDLKPENILMDDeLVIKLSDF 172 green anole
NP_957256     93 LIDSYEStAFIFLVFDLMRRGELFDYLTEKVtLSEKETRSMMRALLEAVQYLHALNIVHRDLKPENILLDDqGHLKLSDF 172 zebrafish
Feature 1         #           ####### #                                                          
2Y7J_A       245 GFSCHLEPGEKLRELCGTPGYLAPEILKCSMDetHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQF 324 human
AAH88919     173 GFSCILKPNEKLRELCGTPGYLAPEILKCSMDetHPGYGKEVDLWACGVIVFTLLAGSPPFWHRRQMLMLRMIMDGRFQF 252 African clawed ...
XP_003227745 173 GFSCHLEPGEKLRELCGTPGYLAPEILKCSMDetHPGYGKEVDLWACGVIFFTLLAGSPPFWHRKQMLMLRMIMEGQYQF 252 green anole
NP_957256    173 GFSVQLGPDEKLRELCGTPGYLAPEILKCSMDetHEGYGKEVDLWACGVILFTLLAGSPPFWHRKQLLMLRMIMEGRYQF 252 zebrafish
Feature 1                                               
2Y7J_A       325 SSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFF 363 human
AAH88919     253 GSPEWDDRSSTAKDLISRLLEVCPEKRLTAEQALQHSFF 291 African clawed frog
XP_003227745 253 GSPEWDDRSDTVKDLISRLLKVDPAERLTAEQALQHSFF 291 green anole
NP_957256    253 SSPEWDDRSDTVKDLISRLLVVEPTIRLTAEQALAHPFF 291 zebrafish

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