2FLO,1U6Z,6PBZ


Conserved Protein Domain Family
ASKHA_NBD_EcPPX-GppA-like
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cd24053: ASKHA_NBD_EcPPX-GppA-like 
nucleotide-binding domain (NBD) of Escherichia coli exopolyphosphatase (EcPPX), guanosine pentaphosphate phosphohydrolase (EcGppA) and similar proteins
The PPX/GppA family proteins play essential roles in bacterial survival and metabolism. Guanosine pentaphosphate (pppGpp) phosphohydrolase (GppA; EC 3.6.1.40) plays a key role in (p)ppGpp homeostasis. It specifically catalyzes the conversion of pppGpp to ppGpp (guanosine tetraphosphate). Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX; EC 3.6.1.11), which mediates the metabolism of cellular inorganic polyphosphate. Especially, it is responsible for the maintenance of appropriate levels of cellular inorganic polyphosphate (PolyP). The family corresponds a group of proteins similar to Escherichia coli exopolyphosphatase (EcPPX) and guanosine pentaphosphate phosphohydrolase (EcGppA). Unlike other PPX/GppA family members containing one PPX/GppA homolog, E. coli possesses two homologs, EcGppA and EcPPX.
Links
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Statistics
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PSSM-Id: 466903
Aligned: 39 rows
Threshold Bit Score: 276.723
Created: 19-Dec-2022
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is located in a cleft between domains I and II of N-terminal domain (NTD) of E. coli exopolyphosphatase.
  • Structure:1U6Z; Escherichia coli exopolyphosphatase binds sulfate Ions in its active site, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             ###                     # #                                            #   
2FLO_A        25 AAVDLGSNSFHXVIARVVDGax-qIIGRLKQRVHLAdgLGPDNXLSEEAXTRGLNCLSLFAERLQgfspASVCIVGTHTL 103 Escherichia col...
1U6Z_A        14 AAVDLGSNSFHMVIARVVDGam-qIIGRLKQRVHLAdgLGPDNMLSEEAMTRGLNCLSLFAERLQgfspASVCIVGTHTL 92  Escherichia coli
6PBZ_A         9 AAIDLGSNSFHMLVVREVAGsi-qTLTRIKRKVRLAagLNSENALSNEAMERGWQCLRLFAERLQdippSQIRVVATATL 87  Escherichia col...
KRO71349      11 ACIDLGSNSFHLLIAQWTPEri-eIIERCSERVQLGegVRASGAISPAAFERGLDCLKRFDELLGrypvARYWALGTNTF 89  OM182 bacterium...
OIQ46683       8 VAIDIGSTNIQMAIAKEHAGrl-iIVERQTQRINLAlgLDDEKKLSSTSIESAITCLREFSCAFSrlpqTDVRIVATHSL 86  Gammaproteobact...
RPG19429      11 AAVDLGSNSFHMTVARLTPSgl-qVLVRDRERVRLAsgLNRKGVLGNKSIQRGIETLKRFDGRLSgvatEDIRVVATHTL 89  Oceanospirillal...
RZV50691      18 AAVDIGSNSIHLIVARAVQGsl-qTVASHRERVQLArnMGANYELDAAAIQRGIDCLQRMGAMLAdaqpDKRRAVATYAV 96  Pseudomonadales...
TNF33882       7 AAIDLGSNSFHLLIATIRDGri-ePLERFSEKIQLGqhVSETGQLCPAAIRRGLKCLGDFSAALQrhpaERVRVVGTQAL 85  Gammaproteobact...
RPG51479      23 AVVDIGSNSIHLEVAFRRADrplhVVHERKLKCQLAagLGPDGELGGNAIERGLEALAVVKSDLDrlapDFVAVVATHAL 102 Gammaproteobact...
WP_011235237  16 AALDIGSNSFHLIIARLVEGql-qPIFKFKQPVQLGe-GLSNRHLSDAALYRGVEALRQCAVRLEgfhrDTVKVVATHTL 93  Idiomarina loih...

Feature 1                                    #                        ####                       
2FLO_A       104 RQAlNATDFLKRAEKViPYPIEIISGNEEARLIFXGVEHTqpe-kgRKLVIDIGGGSTELVIGENf--ePILVESRRXGC 180 Escherichia col...
1U6Z_A        93 RQAlNATDFLKRAEKViPYPIEIISGNEEARLIFMGVEHTqpe-kgRKLVIDIGGGSTELVIGENf--ePILVESRRMGC 169 Escherichia coli
6PBZ_A        88 RLAvNAGDFIAKAQEIlGCPVQVISGEEEARLIYQGVAHTtgg-adQRLVVDIGGASTELVTGTGa--qTTSLFSLSMGC 164 Escherichia col...
KRO71349      90 RVAsNAQAFIRAADQL-GIHISPITGTQEAVLIYAGVLSGlpksaaRRLVIDIGGGSTEVIVGHGs--aQLFTESLAVGS 166 OM182 bacterium...
OIQ46683      87 RQArNKSQFIGAAAKVmPYPIDIIDGKAAAHLIFNGVAHThql-dsRALIIDIGGGSTELIIGKQf--sPSLTSSLPIGS 163 Gammaproteobact...
RPG19429      90 REAkNAHEFLDLAAEHfRAQINVISGPEEARLIFQAVAHTqst-egRFLVFDIGGGSTEFAVGIDf--eTEFLSSRTLGC 166 Oceanospirillal...
RZV50691      97 REAiNRDVFLRLAEAAlGCDVEVISGREEARLIFQAIAHTqkl-eqSVLVIDIGGGSTELAIGAGf--dPDFCASCRMGC 173 Pseudomonadales...
TNF33882      86 RAAsNRQQFLDEASRVlPVPVDIISGEEEAQLIWLGLVGSlpatsrSRLVIDIGGGSTELAYGQGq--qLRFLQSMPLGC 163 Gammaproteobact...
RPG51479     103 RTAsNGQSFAEKVEHLlGVPCEVINGTSEAELVLLGTAVTeqv-tdALAVIDIGGGSTEIALGEPlakrSSFSTSLPLGC 181 Gammaproteobact...
WP_011235237  94 RKAkNRKRFLAEAAKVlPFPIEIISGREEARLIFRGVAETssestgSQLVIDIGGGSTEFAIGKNr--qPKFLSSRSIGS 171 Idiomarina loih...

Feature 1                                                            ##                          
2FLO_A       181 VSFAQLYfpgg----vinKENFQRARXAAAQKLEtlt-wqfRIQGWNVAXGASGTIKAAHEVLXEXgek------dgIIT 249 Escherichia col...
1U6Z_A       170 VSFAQLYfpgg----vinKENFQRARMAAAQKLEtlt-wqfRIQGWNVAMGASGTIKAAHEVLMEMgek------dgIIT 238 Escherichia coli
6PBZ_A       165 VTWLERYfadr----nlgQENFDAAEKAAREVLRpva-delRYHGWKVCVGASGTVQALQEIMMAQgm-------deRIT 232 Escherichia col...
KRO71349     167 VAWRDSYfgqahlseqslELAMESALADARAAFArva-pavKAAGWDEAFAASGTIKMLASVCEAHgfg------agQVS 239 OM182 bacterium...
OIQ46683     164 LDIQKRFfntg----qvnHNNYQQASQFINEQLAhit-arfEHFGWQQVLGTSDTIHLVCRALEQLfa-------spLIT 231 Gammaproteobact...
RPG19429     167 VTFTKRFmkk------vnRESFAKVELNTRKVIEpiv-sriRGLGADKVFGTSGSVKSVSALGSYLgh-------gpIIT 232 Oceanospirillal...
RZV50691     174 VSFSEKFlda-----gysAENFQRANRAALQSIEkyl-anlKVCHWGPVYATSGSAKALAQAVEHLggn------gtELT 241 Pseudomonadales...
TNF33882     164 VAFRDHWfadg----rftPTRYQQALDAAHDLLAhgl-adcPADMGVDYYGSSGTVKSIARIAERQgwc-----sagMID 233 Gammaproteobact...
RPG51479     182 VTLRDQFegv------nhPDHFASARAYVRLLLQksplaekCLSGQPYVFATSGTAKALAKLALGCcaptdnelvgyCVS 255 Gammaproteobact...
WP_011235237 172 LAFSKEFfssg----kitPKRFDKAVVSARQHVEpia-nylAEHQVESVFGTSGTIKAIYQWIVKRdge-----qplGIT 241 Idiomarina loih...

Feature 1                                 #  #                                     
2FLO_A       250 PERLEKLVKEVLRHRNFASLslpglSEERKTVFVPGLAILCGVFDALaiRELRLSDgALREGVLYE 315 Escherichia coli O157:H7
1U6Z_A       239 PERLEKLVKEVLRHRNFASLslpglSEERKTVFVPGLAILCGVFDALaiRELRLSDgALREGVLYE 304 Escherichia coli
6PBZ_A       233 LEKLQQLKQRAIHCGRLEELeidglTLERALVFPSGLAILIAIFTELniQCMTLAGgALREGLVYG 298 Escherichia coli K-12
KRO71349     240 QEALLALRGTFIKQAVSGAAla-glKERRQDLLLAGWSVLLGIMQAYsiDKITFSAtALREGMLEF 304 OM182 bacterium BACL3 MAG-120...
OIQ46683     232 LNSLVKLRKKLLFWHAVDSItlhgfTSDEKWQLSSAVCILESIVKQLniTQLTYCNaALKDGVLYE 297 Gammaproteobacteria bacterium...
RPG19429     233 PESLESCKNFMLMDMNRRTTdipdvSLERMQILPAGIGIISAIVDELrlSQVYFCDaALREGVLYD 298 Oceanospirillales bacterium T...
RZV50691     242 LDQLVALRGEIIRRGNVAWLteygiSDNRMPLLAGGLAILIACLQCLgiSELAYKDvALREGVLYE 307 Pseudomonadales bacterium
TNF33882     234 REAIDRLRTEVLRLGKSSRLkfdglRESRRSVLVPGLVILEAAMEELgmDYLVYSPsALREGMIFN 299 Gammaproteobacteria bacterium
RPG51479     256 RRRLEAAAKLLTTSNEFNRLdy-giGVKRSELLGSGLSVMLEVMSYLgvDSFRYSRsSVRDGVLYK 320 Gammaproteobacteria bacterium...
WP_011235237 242 YSQLQGCRTDLIAAESLKKLnkewvSPERRESIAGGVAVLLGIFEELgiERLVPHDsALREGVLYE 307 Idiomarina loihiensis

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