Conserved Protein Domain Family
M20_Acy1_YkuR-like

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cd05670: M20_Acy1_YkuR-like 
M20 Peptidase aminoacyclase-1 YkuR-like proteins, including YkuR and Ama/HipO/HyuC proteins
Peptidase M20 family, aminoacyclase-1 YkuR-like subfamily including YkuR and Ama/HipO/HyuC proteins, most of which have not been well characterized to date. N-terminal acetylation of proteins is a widespread and highly conserved process that is involved in the protection and stability of proteins. Several types of aminoacylases can be distinguished on the basis of substrate specificity; substrates include indoleacetic acid (IAA) N-conjugates of amino acids, N-acetyl-L-amino acids and aminobenzoylglutamate. ACY1 breaks down cytosolic aliphatic N-acyl-alpha-amino acids (except L-aspartate), especially N-acetyl-methionine and acetyl-glutamate into L-amino acids and an acyl group. However, ACY1 can also catalyze the reverse reaction, the synthesis of acetylated amino acids. ACY1 may also play a role in xenobiotic bioactivation as well as in the inter-organ processing of amino acid-conjugated xenobiotic derivatives (S-substituted-N-acetyl-L-cysteine). ACY1 appears to physically interact with Sphingosine kinase type 1 (SphK1) and may influence its physiological functions; SphK1 and its product sphingosine-1-phosphate have been shown to promote cell growth and inhibit apoptosis of tumor cells. Strong expression of the human gene and its mouse ortholog Acy1 in brain, liver, and kidney suggest a role of the enzyme in amino acid metabolism of these organs.
Statistics
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PSSM-Id: 349920
Aligned: 18 rows
Threshold Bit Score: 460.191
Created: 26-Sep-2007
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
metal bindingputative dimer
Feature 1: metal binding site [ion binding site], 5 residue positions
Conserved feature residue pattern:C H E H HClick to see conserved feature residue pattern help
Evidence:
  • Comment:defined by homology to related sub-groups
  • Comment:conserved metal-coordinating cysteine in this subfamily differs from archetypal M20 motif

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
ABI26326       6 ELIQIRRHLHQIPELALHEFDTHQYLVETVAsfnqaFLEVRTFKelpTALMVLVHGQn-PQRTIGYRTDIDALPVEEqtg 84  Lactobacillus r...
YP_805402      5 DLITLRRQLHQIPELALQEKETHALLLKTIQglpqtYFTIRTLPdlpTALLVKVQGRd-PQRTIGYRTDIDALPVTEktg 83  Lactobacillus c...
B1YJ90         3 YAIEMRRELHKIPEPGFKEFKTQAFILDQIRsypedRVSYDTFE---TGVFVRVKGLt-GNRTIGYRADIDGLPIEEatg 78  Exiguobacterium...
B1MZM9         8 TLQTFRRELHQIPETALEEFKTHDYLLTKLKswqqdYMTIKTVEalpTAILVYFQGTn-PVRTIGYRTDIDALPIQEatg 86  Leuconostoc cit...
A0AHD2         4 EFIAIRRDLHQIPETGYKELKTQAYLLAYISklpnkHLEVKKWR---TGILVLVKGTk-PEKTIGYRTDIDALPITEetg 79  Listeria welshi...
Q5WF94         5 DLIHIRRAFHQIPELGFNEFKTQKLLLDTISnmeqtRLQIKTWK---TAVFVRVEGR--QDYTIAYRADMDGLPITEetg 79  Bacillus clausi...
O34916         5 ELIAIRRDLHRIPELGFQEFKTQQYLLNVLEqypqdRIEIEKWR---TGLFVKVNGTa-PEKMLAYRADIDALSIEEqtg 80  Bacillus subtil...
EBA40733      34 ELRRYRRDLHRIPELDFDLPQTIAYIEGVLAp---lACEVTHPCp--SCVCAFFDAGvgAARATAIRADMDALPIAEatg 108 Collinsella aer...
YP_001089738  10 QLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDk---lGIEYEVYLe--TGIVGVIEGKr-PKKDIAFRADIDGLHSEE--- 80  Clostridium dif...
Q8ERA3         4 QLEQIRRDLHQIPELGFQEFKTQAYLLERINeiateNVEIKKWS---TGILVYVHGKs-PARKIGFRADIDGLPILEqtn 79  Oceanobacillus ...
Feature 1                     # #                                     #                          
ABI26326      85 lpyssthpGIMHACGHDIHMTVALGVLNYFSEHQPQ------DNILFFFQPAEESENGGKRAYELGLFsGKWKPDEFYGL 158 Lactobacillus r...
YP_805402     84 lpfaskhsGIAHACGHDIHMTVALGILSYFAEHQPK------DNLIFFFQPAEESKNGGKLAYDMGAFtGDWHIDEFYGL 157 Lactobacillus c...
B1YJ90        79 lpfcsehpGFMHACGHDVHASIALGLLRRIVELPVM------DDVVFLFQPAEEGPGGAEPMIKSPLF-EKYRPSEMYGL 151 Exiguobacterium...
B1MZM9        87 ldfasqhpGKMHACGHDVHMTMALGLAQYFSQHQPK------DNLIIFFQPAEEAESGGKVAYDMGLFeGKWRPDEFYGI 160 Leuconostoc cit...
A0AHD2        80 lpfesnhvGNMHACGHDLHMSIALGVLTHFASKPAK------DNLLFVFQPAEEGPGGAKPIMESEEF-AAWRPDTIYGL 152 Listeria welshi...
Q5WF94        80 ysfaskheGAMHACGHDFHMTIALGLLDHFASHEPE------CHLLFIFQPAEEGPGGAKPIIEADVL-GAWQPDEIYAL 152 Bacillus clausi...
O34916        81 lpfasehhGNMHACGHDLHMTIALGIIDHFVHHPVK------HDLLFLFQPAEEGPGGAEPMLESDVL-KKWQPDFITAL 153 Bacillus subtil...
EBA40733     109 aafasihpGKMHACGHDGHMAMALAAATYVDRTIREqpgairRNVLFVFQPAEETTGGAKTVCESGVF-ERYGADRIFGF 187 Collinsella aer...
YP_001089738  81 --------GVKHLCGHDGHATILLGLIEYLNDNKEDl----nDNIVFIFQPAEEGPGGAKALIDEGVL-RKYNIDEIYGL 147 Clostridium dif...
Q8ERA3        80 lpyaslheGRMHACGHDLHMTIALGALEKLIQDPIN------DDVIFVFQPAEEGPGGAKPMLESEEF-QQWKPDMMFAL 152 Oceanobacillus ...
Feature 1        #                                                                               
ABI26326     159 HDNPDLPAGAIGCRMGTLFAGTTEVNIDLNGKGGHAAYPQn------ANDTVVAAASLILQVQTVISRSIDPiqsgVITL 232 Lactobacillus r...
YP_805402    158 HDRPDLPAGTISTRLGTLFAGTTEINVDLIGKSGHAALPQn------ANDMIVAAASFISQIQTVVARNVGPtdsaVITF 231 Lactobacillus c...
B1YJ90       152 HVAPEYPVGTIASRPGVLFASAREVHITIYGQSGHAAFPHl------TIDTVVAQAALIMQLQTIVSRSINPmncsVITI 225 Exiguobacterium...
B1MZM9       161 HDQPNLPAGTLSTLAGTLFAGTAELKVDVIGTGGHAAYPHl------AKDPIVIAAELIIQLQTVVSRSVDPiaggVVSV 234 Leuconostoc cit...
A0AHD2       153 HIAPEYKVGEIAIKPGLLFANTSELFISFKGKGGHAAYPHl------ANDMVVAASAFVGQMQTIISRNIDPmdsaVITI 226 Listeria welshi...
Q5WF94       153 HIDPNLPVGSIATKPGLLFANTSELFIDFQGKGGHAAYPHt------ANDMVVACAHFVTQVQTVVARNIDPldsaVVTL 226 Bacillus clausi...
O34916       154 HIAPELPVGTIATKSGLLFANTSELVIDLEGKGGHAAYPHl------AEDMVVAASTLVTQLQTIISRNTDPldsaVITV 227 Bacillus subtil...
EBA40733     188 HVWPDLPAGTLASCSGPLLARSSETHIHIHGTSIHIAKTYgvpveesHDVALAAAKFLVAERELMDELGADEp--cIGKF 265 Collinsella aer...
YP_001089738 148 HIYPELPEGYVGTREGYFMAQIGDIDIEIVSKSGHGAMPQn------GIDGIVIASNFVSSLQTIVSRNISPidnaVLTI 221 Clostridium dif...
Q8ERA3       153 HIAPELPVGTVSSKAGLLFANTSELFIDFEGVGGHAAYPHl------TKDMTVAASNFVVQLQQIVSRGLNPldgsVITI 226 Oceanobacillus ...
Feature 1                                                                                        
ABI26326     233 GKIDGGTiRNVIAGHTRIEGTIRGLTQTmIETIDDRLKDVCEGIGrsfNMDVSLELNqggYWPVENNPELTKRFIHYMEE 312 Lactobacillus r...
YP_805402    232 GLMRAGTiRNVIAGRAHLEGTLRGFTQQqIDFLQQRIRDIGQGIAasfNCQVKVALNqggYYPVENNDKLTKNFIQVMKD 311 Lactobacillus c...
B1YJ90       226 GKVDAGIrENVIAGRALLDGTMRALNGTdMEKLEQRVRDIIRGIEasfGVKIDLQFGn-rYYEVVNDQRVVDKFSSFVKM 304 Exiguobacterium...
B1MZM9       235 GVINGGFaNNVIPDQVHFEGTVRSMTRTgLETMLTRIRKIAEGLAianEVTINVSLEsgsYLPVENDPILATQVINFMQK 314 Leuconostoc cit...
A0AHD2       227 GRIHGGEiQNVIAETAFLDGTIRTLSPEtMEIVWTRLKQLAKGWEeayQCEVEFHPGs-dYYQVDNEPLETEEFIRFLKE 305 Listeria welshi...
Q5WF94       227 GVIAGGTkQNVIAATARLEGTIRTLSMAsMEVVKSRLEAIAAGIEasfACKIAIDYGs-nYCEVYNDPELAEAFAAFSKT 305 Bacillus clausi...
O34916       228 GTITGGSaQNIIAETAHLEGTIRTLSEEsMKQVKERIEDVVKGIEigfRCKGKVTYPs-vYHQVYNTSGLTEEFMSFVAE 306 Bacillus subtil...
EBA40733     266 GLLQAGTvCNAVAGEAHVAGSLRVFTDAmFDRAREGVRRALDEACtatGCTYDLDFA---EGYPPVDNDPELFAHIAPAL 342 Collinsella aer...
YP_001089738 222 GRIEGGArRNIIAENIKMEGTMRCFSPEvYEKMKLRVRELARGFElayNCKVNINIId-dYIAVKNDKDLYQEFVEAIGS 300 Clostridium dif...
Q8ERA3       227 GKMESGYvQNAIAETARLEGTIRSTDADaIDMIKSKLNRLMKGFEisyDCTIKVDYGa-nYYQVVNDATYVQQFENVINQ 305 Oceanobacillus ...
Feature 1                                                                 #                      
ABI26326     313 tptvNFIETEPAMTGEDFGYLLAKFPGTMFWLGVED-----------------DSQLHSATLTPNEKAIKRGVDAITGFL 375 Lactobacillus r...
YP_805402    312 dpdvTFVDTKPVMTGEDFGYLLNKIPGTMFWLGVNDp----------------DSQLHAADFSPDEAALVPGVTAVVHFL 375 Lactobacillus c...
B1YJ90       305 n--aNYIECDAAMTGEDFGFMLKEIPGMMFWLGVNNa----------------TSGLHQPTLNPDEEAIPFVINLLDHYF 366 Exiguobacterium...
B1MZM9       315 qsdiNFELAQPAMTGEDFGYLLQHIPGVMLWLGVND-----------------SHPLHSAQLTIDESAILPGYNALKSFL 377 Leuconostoc cit...
A0AHD2       306 hypeSYVPARSAMTGEDFGYFLSEIKGFMFWLGVDS-----------------EYSLHHAKLNPKEEAIPFAIDVLINFL 368 Listeria welshi...
Q5WF94       306 rkgiTFVEAEEAMTGEDFGYFLKQYPGVMFWLGVDS-----------------PYGLHDSRLQPSEEAIGIAIEHMVAFL 368 Bacillus clausi...
O34916       307 hqlaTVIEAKEAMTGEDFGYMLKKYPGFMFWLGADS-----------------EHGLHHAKLNPDENAIETAVHVMTGYF 369 Bacillus subtil...
EBA40733     343 p--eLQLVDEPLLIAEDFAFYQRHLPGVFFLLGTGVpegqenpidaddcpayaMSALHTDTMLFDEEILLKGLDVYKRLL 420 Collinsella aer...
YP_001089738 301 d---TVVELEPLMISEDFSYYQKEVPGLFFMLGSRNeek------------gfVNGLHNINFNFDEKICVNALNVYSKLL 365 Clostridium dif...
Q8ERA3       306 tqtiTYQQADAAMTGEDFGDMLKEIPGFMFWLGVDS-----------------SYGLHNARLNPKEEAIDVGVNAVISMI 368 Oceanobacillus ...
Feature 1         
ABI26326     376 E 376 Lactobacillus reuteri
YP_805402    376 E 376 Lactobacillus casei ATCC 334
B1YJ90       367 R 367 Exiguobacterium sibiricum 255-15
B1MZM9       378 L 378 Leuconostoc citreum KM20
A0AHD2       369 E 369 Listeria welshimeri serovar 6b str. SLCC5334
Q5WF94       369 S 369 Bacillus clausii KSM-K16
O34916       370 S 370 Bacillus subtilis subsp. subtilis str. 168
EBA40733     421 L 421 Collinsella aerofaciens ATCC 25986
YP_001089738 366 K 366 Clostridium difficile 630
Q8ERA3       369 T 369 Oceanobacillus iheyensis HTE831

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