6MSO


Conserved Protein Domain Family
Fumerase
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pfam05681: Fumerase 
Fumarate hydratase (Fumerase)
This family consists of several bacterial fumarate hydratase proteins FumA and FumB. Fumarase, or fumarate hydratase (EC 4.2.1.2), is a component of the citric acid cycle. In facultative anaerobes such as Escherichia coli, fumarase also engages in the reductive pathway from oxaloacetate to succinate during anaerobic growth. Three fumarases, FumA, FumB, and FumC, have been reported in E. coli. fumA and fumB genes are homologous and encode products of identical sizes which form thermolabile dimers of Mr 120,000. FumA and FumB are class I enzymes and are members of the iron-dependent hydrolases, which include aconitase and malate hydratase. The active FumA contains a 4Fe-4S centre, and it can be inactivated upon oxidation to give a 3Fe-4S centre.
Links
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Statistics
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PSSM-Id: 461713
Aligned: 347 rows
Threshold Bit Score: 194.939
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
6MSO_A            93 TLLSSTAFDDVEHLLRSSHLMSLRKIF---DDpEA---SDNDkfvALQLLKNANISSARLLPGCQDTGTAIIAGYRGDQV 166
jgi:Spica_1190    41 TKLAEEAFVRISYYLRPSHLKLWSEQL---NSnTL---TANDrlvLETLLKNALISAEGKLPLCQDTGTATIIGWKEESV 114
XP_001608904      86 QKLTEQAFVEIMHYLRPQHLQQLRNIL---DDhEA---SNNDrfvAMQLLKNACVASGKVLPGCQDTGTAIVLGKRGNRI 159
WP_014547259       5 EQKVCECLIQAGSTFREDQKEAYRRAI---AK-EP---GTNScwvMQQVLDNALVAEKNRGPLCDDTGIPHIFIEVGPNR 77  Fibrobacter...
CCY70083           7 vTKVCDTLVKAGSTFLGDKKEAYKKAI---AK-EA---NEQAkwtMEKILENAYVAEKNQSPLCDDTGIPHLVLEVGPGK 79 
agres:mru_0690     4 IKKVEDAVVKAGSGYSEDRLTAYENAL---RL-EEekgNENAvwaLEQMIENFKVAKENKLPLCDDTGIPHIIIEIGYNR 79 
NZAGRC:Abm4_0418   4 INKIADGVIKASTTYSQDRIDGFKRAL---KL-EEelsNDNAvwaLEQMIRNESIASLRKLPLCDDTGIPHILVELGENR 79 
WP_023992715       6 RDLVKDAVITASTTFREDQIQAYQQAI---QK-EE---NDNArwvLKLLLENARIAQSKKVPLCDDTGIPHVLVELGPET 78  Methanobact...
WP_013413748       4 VKKVKKAVIKCSTEYSEDQIKAYKNAI---KN-EN---NENAvwvLKLLLKNAKIAKKKRRPLCDDTGIPHVYIEIGKKS 76  Methanother...
WP_015711790      43 SQAAEEGFRRLSFFFRESHLAMLAEQLalaENdKK---PGNDgfvLNALLRNAVIAAKGELALCQDTGTAVIYGWKSENV 119 Treponema a...

6MSO_A           167 FvpGN--D-EEALSRGVYDIFQKrnFRYSQNvPLsmY-DEKNTGT---NLPAQIDLYASKg-----ME------------ 222
jgi:Spica_1190   115 R--TGaeDgEA-LSNGVLQAYTGhpLRASQVgFRs-LfDEFDTGN---NLPAQIHIEAVRdns-egPE------------ 174
XP_001608904     160 Y--SEg-DeAA-IAKGVYEAYVNrkFRYSQMaPIt-MfDEVSTKC---NLPAQIEIYSKEg-----SF------------ 214
WP_014547259      78 Qv-TG--DvLAQIHAGIEAGLRKlpGRPMAIlGDd-Y-HRIDQSGglnPDSGALAPS--PilikpvEEd----------- 139 Fibrobacter...
CCY70083          80 Ai-TG--EmIESIKEGVRCGLRKlpGRPMAIkGNd-S-QRIDQSQgleDDPGMLEAA--PilirniKEd----------- 141
agres:mru_0690    80 Ei-PK--NfFNDINAGIANGLDKlpGRPMAVkGNd-I-ERIEQSKglyAESNMMKPA--Sfl--ieEKddstygrkigid 150
NZAGRC:Abm4_0418  80 Sl-PE--GfIRDIKEGLKQGLDDlpGRPMAVkGNp-L-ERIEQSKglyDEPSMMLPA--PisidsfDKnnrsys---rdl 149
WP_023992715      79 Sf-KP--DlFHQIEEGVADGLRKlpGRPMAVcGDd-I-ERVEQSRglsSDPGKVISP--SilvdkmDGn----------- 140 Methanobact...
WP_013413748      77 Ei-SS--TfFQKIRRGIAEGLRElpGRPMALkGNd-I-ERIEQSKgiyEDPGKVVPP--SffidnnKEen---------- 139 Methanother...
WP_015711790     120 F--TGsgDdDAWLESGIGAAYKKnnLRLSQIgASs-FlDEFNTGN---NLPAQIQIHASGk----nPV------------ 177 Treponema a...

6MSO_A           223 ---YSFMFVAKGGGSANKSFLlqetkS-----VL--------NPKSLRNFLKEKLAMFGTSACPPYHVAVVIGGTSAEMT 286
jgi:Spica_1190   175 ---YRFLFIAKGGGSANKTAL-----FqmtkaLL--------EPETFKAFLKEKIAALGTAACPPYRLAVVVGGTSPEEN 238
XP_001608904     215 ---YDFLFIAKGGGSANKTFL-----YqqtkaVL--------NPNVLIDFLMDQIKTIGTSACPPYHLAVVIGGLSAEMT 278
WP_014547259     140 --vLRLTILMLGGGPAIRGIT-----Q-----RVfhkhsvqvViDEIVARAIPAVSQLG---CSPCTLAVGIGRSQFEAT 204 Fibrobacter...
CCY70083         142 --kIKLNILMFGGGPAIRGRT-----Y-----RVfhkhdtnvVlDEIVSWAKEATKQLG---CTPCTLGVGVGRSNYEAA 206
agres:mru_0690   151 ddkIRINVLLEGGGPEIRAKT-----Y-----RVfhkrdsniVfNEALSWLKESLSMLG---CTPSIPAIGIGRSHFEAN 217
NZAGRC:Abm4_0418 150 enqIHIHILLLGGGPEIRSKT-----Y-----RVyhqhdrkiViNETIDWLKESLKMLG---CTPSIPAIGIGRSHYEAS 216
WP_023992715     141 --gLKIHVLMLGGGPEIRSHT-----Y-----RVfhqrdhgnLfKEALTWMRSEIPKLG---CTPCIPALGIGRTHFEAS 205 Methanobact...
WP_013413748     140 --gTKIHIMMLGGGPEIRART-----K-----KVfhehdyrkVfKEVIRWIIVEAKSLG---CTPCVPAIGIGRTHYEAT 204 Methanother...
WP_015711790     178 ---YRFLFIAKGGGSSNKTSL-----FsmtksLL--------EEQAFEKFLEEKIKALGTAACPPYRLAVVVGGTSPEFN 241 Treponema a...

6MSO_A           287 MKVLKyASchyYDDLI--TKP-DMKTgyt----frdLELEEEVLKVCQNIGMGAQ-FGGKYYAHDVRVIRMPRHGASCP- 357
jgi:Spica_1190   239 LSILKlATtelLDTAPrmESAfKNNGshwl---yrdTEWESILMNITRESGLGAQ-FGGSLLALDARLIRLPRHAGSCP- 313
XP_001608904     279 LKTVKlLSckyLDGLP--TEG--TQFgta----frdIELENEILNRTRSIGIGAQ-FGGKYFCHDVRVVRLPRHGASCP- 348
WP_014547259     205 SMMME-AM----------AKGnYDFQ----------SEFEKTITERVNESNVGALgLGGKTSVLATFAKVGPQRASGVRi 263 Fibrobacter...
CCY70083         207 SMMLE-AL----------VDGtYDRQ----------SPMEKYITEKVNEERTGPLgLGGDTTVLATFMKVGPQRASGVRv 265
agres:mru_0690   218 SLMLR-AM----------VHGnLNDQ----------SDIEKYIADELNKTNIGPLgLGGVTTVLGSFVNIGYQRASGVRi 276
NZAGRC:Abm4_0418 217 HLMLK-AM----------VYGnLNYQ----------SNTENFITNELNKLNIGPMgLKGHTTVLGTFLNIGPQRASGVRi 275
WP_023992715     206 SQMLK-AM----------VYSnLNHQ----------SSIEKKITDSLNRTNVGALgLGGSVTALGSMIKIGPQRASGVRi 264 Methanobact...
WP_013413748     205 SLMLK-AI----------AHGkLDQQ----------SELEEYITNSVNSSGIGPLgLGGSTTALGTLIKIGPQRASGVRi 263 Methanother...
WP_015711790     242 LEACKlASaeaLDSAPyfEPGeDKEKsaslpwirrdKQWEEKAMSIGRRTGLGAQ-FGGSSLLLDARVLRLPRHAASCP- 319 Treponema a...

6MSO_A           358 IGIGVSC-SADRQA 370
jgi:Spica_1190   314 VSIGVSC-SAHRNM 326
XP_001608904     349 IGIGVSC-SADRQI 361
WP_014547259     264 VALRPCCcFEPRRA 277 Fibrobacter succinogenes
CCY70083         266 VCLRPCCcFEPRIA 279
agres:mru_0690   277 VAVRPACfVEPRVs 290
NZAGRC:Abm4_0418 276 VCARPCCfVEPRIS 289
WP_023992715     265 VCMRPCCcVEPRKA 278 Methanobacterium sp. MB1
WP_013413748     264 VSTRLCCcVEPRKY 277 Methanothermus fervidus
WP_015711790     320 VSIGVSC-AAHRNL 332 Treponema azotonutricium
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