1BKJ,1F5V,2BKJ,1ZCH


Conserved Protein Domain Family
NfsA-like
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cd02146: NfsA-like 
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nitroreductase similar to Escherichia coli NfsA
This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.
Links
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Statistics
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PSSM-Id: 380322
Aligned: 27 rows
Threshold Bit Score: 245.995
Created: 13-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:1BKJ: A vibrio harveyi oxidoreductase homodimer binds two molecules of FMN, contacts at 4A
  • Citation:PMID 8885832
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1               ### #                            ### #                         # #       
1BKJ_A         4 TIETILAHRSIRKFTAVP------ITDEQRQTIIQAGLAASSSSMLQVVSIVRVTDSEKRNELAQFAg-NQAYVESAAEF 76  Vibrio harveyi
1ZCH_A         4 VIKSLTDHRSIRSYTDEP------VAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKISELAg-GQPWIDQAPVF 76  Bacillus subtilis
BAB06672       4 VIQTMMNHRSCRQYSDKP------VPDDHLQAILHAAQWGPSWIHGQQVSVIVIREPNERELFAELVg-GQAHVAQAPIF 76  Bacillus halodu...
EAA66922      37 TISTILEHRSIRAFLPTP------LPPGTLETLIAAGQSASTFSMLQTWSVVAIHDPQHKDAVARVSg-NQDFIRQAPLF 109 Aspergillus nid...
BAC00228      26 TLDVIHQHRSVRRWLDKP------VDDDTIRTIISAAQSAGTSSNKQVISVIVVKDPELRKGLAGITrqMFPHLEQVPAV 99  Corynebacterium...
NP_347355      4 TIDTMKNHRSIRQYLDKE------VPNDLIDEIVKSAQAMPNSINGQQTSVIVVRDKKKREKLAELVg-NQEYVAKAPVF 76  Clostridium ace...
AAQ61161       4 TLQLMHSHRSVRSYQDKP------VAPELLDAVLDAAWKGPTSINGQQISLVVVQDPERRKRIAEIAg-GQPWIAQAPVF 76  Chromobacterium...
AAF10968      10 VRAFYDAHRTVRLYQTQPdgspirMTADELDAVLHAAQRAPTDATAQLYSYIHLTTPELRAQVAELT--GNPHINIASEA 87  Deinococcus rad...
NP_266909      7 TIDLMMNHTSVRNFTEEA------IEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQKDAIYEFQ--PQKSIKNCAAY 78  Lactococcus lac...
YP_005794822  27 LVARLMGHRSVRAYSDRA------VEDDVLLQAIAAAQSASTSSNLQAWSVVAVRDPAKRARLSALCg-NQRHINDAPVF 99  Ketogulonicigen...

Feature 1                                           ##  #                #####                   
1BKJ_A        77 LVFCIDyqrhatinp------dvqadftelTLIGAVDSGIMAQNCLLAAESMGLGGVYIGGLRNSaAQVDELLGLPEnSA 150 Vibrio harveyi
1ZCH_A        77 LLFCADfnrakialedlhdfkmeitnglesVLVGAVDAGIALGTATAAAESLGLGTVPIGAVRGNpQELIELLELPKyVF 156 Bacillus subtilis
BAB06672      77 LVFCADfhraavaaearg-kflaaledvdaLMVGATDVGIALSNAITAAESLGLGTVPIGGIRRQpLRVIETLQLPSyVI 155 Bacillus halodu...
EAA66922     110 LIFCADlhrhanvidkyr-tagkplekidmLIAGVVDASIAAENVAIAAESLGLGICMVGGVRNNaESLVELLHLPEkTF 188 Aspergillus nid...
BAC00228     100 LIWLIDysrisavaared-lptgaldyldeAALGFLDAGIAAQNAAIAAESLGLGTLYLGSVRNDaEAVQKLLGLPPeIV 178 Corynebacterium...
NP_347355     77 LVFVMDfyrtylagektg-lkqvihediegILAGSVDVGIALGASVVAAESLGLGTVPIGGIRKNpEEVIEILGLPKyTF 155 Clostridium ace...
AAQ61161      77 VAVVMDfhktrlgvemag-ekqvihdsveaFAVGSVDAGIALGNMITAAHAAGLGVVPIGGIRRDpQAMIDLLELPElTF 155 Chromobacterium...
AAF10968      88 FIACSDtrrvgkileaa--gyesgswpaiaVHFGIGDIVMSGQNLLTAAEMLGYQGCWIGGVVNNmDALITLLGLPEgVL 165 Deinococcus rad...
NP_266909     79 LVFVGDlnraqkaveld--qgnfqpqgiesLLITSVDAATAGQNALLAAESLGYNGVMVGLIRDQsSEISKVLNLPDyTY 156 Lactococcus lac...
YP_005794822 100 LIWLVDwsrlrriaatqd-qpsdgtdylesYTVGVVDAALAAQNAAVALEALGLGLVYIGGIRNHpEDVAAELGLPSgTF 178 Ketogulonicigen...

Feature 1                          # #                                                           
1BKJ_A       151 VLFGMCLGHPDQn--PEVKPRLPAHVVVHenqyqel---nlddiqsYDQTMQAYYASRtsnqklstWSQEVTg------k 219 Vibrio harveyi
1ZCH_A       157 PLSGLVIGHPADr--SAKKPRLPQEAVNHqetylnqd-eltshiqaYDEQMSEYMNKRtngketrnWSQSIAs------y 227 Bacillus subtilis
BAB06672     156 PISGLCIGYPEEt--VEQKPRLPLDVFTHdgryepl---sqktlaaYDELISGNTKQRtggattttWSDRLIs------f 224 Bacillus halodu...
EAA66922     189 GIVGLAVGYADPtahEDIKPRLPMREILHyetwdsnqqrqdenvamFDDVLSKHYALHsmlg-rkpWSRFAAewna-dgr 266 Aspergillus nid...
BAC00228     179 PVVGLEIGHADPsepAGIKPRLPQEAIVHwdtytek---nlelidsYDRALDTYYSRYgqh---qlWSKQTAhraa-sks 251 Corynebacterium...
NP_347355    156 PMVGLVVGYPADe--SHKKPRVPFESFRHnesydika--vedsinvYDEQMNKYLKEIgraekeinWSTFTStiyq-svy 230 Clostridium ace...
AAQ61161     156 PLVGLVIGHVQDd--ADLKPRLPRASFIHresyqaae--laghiaaYDDTLRRHWQDIgrad-gepWTANTAryyr-qvy 229 Chromobacterium...
AAF10968     166 PFAAITVGRPAEd--APLRPRLPRELVVHtgtyrdg---tpeelraATELMNPIAERPgkp---gdWARLLKmyfgqggg 237 Deinococcus rad...
NP_266909    157 PIFGIALGKAARl--NKVKPRLPLEATAFkekyveq---tsetiekYDQVQEEYAGNRrl----nkWSERIVd------q 221 Lactococcus lac...
YP_005794822 179 ALVGMSIGYPDParpADIKPRLSQATILHnetydsap--eqaavaaYDAAMVDFQRAQgrg--dlpWSATAVqrvkgpgs 254 Ketogulonicigen...

Feature 1              
1BKJ_A       220 LAGESR 225 Vibrio harveyi
1ZCH_A       228 YERLYY 233 Bacillus subtilis
BAB06672     225 YEQPYY 230 Bacillus halodurans C-125
EAA66922     267 MDGRDR 272 Aspergillus nidulans FGSC A4
BAC00228     252 ITKTNR 257 Corynebacterium glutamicum ATCC 13032
NP_347355    231 YPKVKG 236 Clostridium acetobutylicum ATCC 824
AAQ61161     230 FPKVAP 235 Chromobacterium violaceum ATCC 12472
AAF10968     238 MEVREP 243 Deinococcus radiodurans R1
NP_266909    222 WGQPEI 227 Lactococcus lactis subsp. lactis Il1403
YP_005794822 255 LSGRER 260 Ketogulonicigenium vulgare

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