1OC3,1NM3,1H4O,1XIY,1TP9


Conserved Protein Domain Family
PRX5_like
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cd03013: PRX5_like 
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Peroxiredoxin (PRX) family, PRX5-like subfamily; members are similar to the human protein, PRX5, a homodimeric TRX peroxidase, widely expressed in tissues and found cellularly in mitochondria, peroxisomes and the cytosol. The cellular location of PRX5 suggests that it may have an important antioxidant role in organelles that are major sources of reactive oxygen species (ROS), as well as a role in the control of signal transduction. PRX5 has been shown to reduce hydrogen peroxide, alkyl hydroperoxides and peroxynitrite. As with all other PRXs, the N-terminal peroxidatic cysteine of PRX5 is oxidized into a sulfenic acid intermediate upon reaction with peroxides. Human PRX5 is able to resolve this intermediate by forming an intramolecular disulfide bond with its C-terminal cysteine (the resolving cysteine), which can then be reduced by TRX, just like an atypical 2-cys PRX. This resolving cysteine, however, is not conserved in other members of the subfamily. In such cases, it is assumed that the oxidized cysteine is directly resolved by an external small-molecule or protein reductant, typical of a 1-cys PRX. In the case of the H. influenza PRX5 hybrid, the resolving glutaredoxin domain is on the same protein chain as PRX. PRX5 homodimers show an A-type interface, similar to atypical 2-cys PRXs.
Links
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Statistics
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PSSM-Id: 239311
Aligned: 64 rows
Threshold Bit Score: 146.938
Created: 3-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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catalytic triaddimer interface
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:Deprotonation of the catalytic cysteine thiol is facilitated by H-bonding with the threonine or serine hydroxyl group, and the positive charge of the arginine sidechain.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                             #  #                    
1OC3_B     16 VGDAIPAVEVFEge--------------pgNKVNLAe---lFKGKKGVLFGVPGAFTPGCSKThLPGFVEQAEALKak-g 77  human
1NM3_B      5 EGKKVPQVTFRTrqg------------dkwVDVTTSe---lFDNKTVIVFSLPGAFTPTCSSShLPRYNELAPVFKky-g 68  Haemophilus influe...
CAE76545    6 AGDSFPEGVTFTyvapsgdt--dltvcglpIQYDASk---aFANKKVVLVAVPGAFTPTCQQQhVPSYLAKLEEFKsk-g 79  Neurospora crassa
EAA47467    6 VGDAFPDGVSFTyvptggkke-dvtacgngIKYNASt---eAKDKKIVIVSVPGAFTPTCQEQhLKSYVEKKDELKak-g 80  Magnaporthe grisea...
O43099      6 AGDSFPSDVVFSyipwsedkg-eitacgipINYNASk---eWADKKVILFALPGAFTPVCSARhVPEYIEKLPEIRak-g 80  Aspergillus fumigatus
EAA72267    6 VGADFPEGVFFSyvqptpeia-efstcgapTNYNASq---eFKSKKVVLISVPGAFTPTCSAShVPSYVENIEQIKak-g 80  Gibberella zeae PH-1
P38013      5 VNKKFPAGDYKFqyiaisqsdadsesckmpQTVEWSkl--iSENKKVIITGAPAAFSPTCTVShIPGYINYLDELVkeke 82  baker's yeast
Q9M7T0     39 ITSAAPGVSLQKarswd---------egvsSKFSTTplsdiFKGKKVVIFGLPGAYTGVCSQQhVPSYKSHIDKFKak-g 108 thale cress
P56577     10 KGNEIPDTLMGYipwtpeld--sgevcgipTTFKTRd---eWKGKKVVIVSIPGAYTPICHQQhIPPLVKRVDELKak-g 83  Pityriasis (Tinea)...
XP_455979   2 SSDTLPNTRFQYiriipsq----egteacsAPVETSf---tEYKGTVVITGAPAAFSPTCSVShIPGYVQKLNQLVda-g 73  Kluyveromyces lact...

Feature 1                                                                   #                 
1OC3_B     78 VQVVACLSVNDAFVTGEWGRAHKae--gKVRLLADPTGAFGKETDLLLddslvsifgnRRLKRFSMVVQDGIVKALNVEP 155 human
1NM3_B     69 VDDILVVSVNDTFVXNAWKEDEKs---eNISFIPDGNGEFTEGXGXLVgked--lgfgKRSWRYSXLVKNGVVEKXFIEP 143 Haemophilus influe...
CAE76545   80 VDQIIFIASNDAWVMAAWGKANGvk-deSILFMSDADCKFSKSIGWTQg---------DRTKRYAIVIDHGKVTYAELDE 149 Neurospora crassa
EAA47467   81 VDKVVFIAYNDHWVMSAWGKANDiy-ddFIIFASDDGISFSKSIGWNLgd-------sGRTARYAIVVDHGKVTYAEKEE 152 Magnaporthe grisea...
O43099     81 VDVVAVLAYNDAYVMSAWGKANQvt-gdDILFLSDPDARFSKSIGWADe--------eGRTKRYALVIDHGKITYAALEP 151 Aspergillus fumigatus
EAA72267   81 VDQVIVVAVNDAFVMHGWAKANGit-ddKILFMSDHNAKFSTSIGWNLg---------ERTGRFAVVIDHGKITYASSDE 150 Gibberella zeae PH-1
P38013     83 VDQVIVVTVDNPFANQAWAKSLGvkdttHIKFASDPGCAFTKSIGFELavg----dgvYWSGRWAMVVENGIVTYAAKET 158 baker's yeast
Q9M7T0    109 IDSVICVSVNDPFAINGWAEKLGak--dAIEFYGDFDGKFHKSLGLDKdlsa--allgPRSERWSAYVEDGKVKAVNVEE 184 thale cress
P56577     84 VDAVYVIASNDPFVMAAWGNFNNak--dKVVFATDIDLAFSKALGATIdlsa--khfgERTARYALIIDDNKIVDFASDE 159 Pityriasis (Tinea)...
XP_455979  74 ASQVFVVTADNPFANQQWAKTLGvkdtdKIKFITDAGAKFSQSLGFALpie----sgvFWASRYLVIAKDGKIVYQAVEE 149 Kluyveromyces lact...

Feature 1                      
1OC3_B    156 dg--tGLTCSLAPNIIS 170 human
1NM3_B    144 nepgdPFKVSDADTXLK 160 Haemophilus influenzae
CAE76545  150 vp--kSIANTGADGVLA 164 Neurospora crassa
EAA47467  153 a---gGIAVSGAEAVLA 166 Magnaporthe grisea 70-15
O43099    152 ak--nHLEFSSAETVLK 166 Aspergillus fumigatus
EAA72267  151 p---gSIEKSGALGVLP 164 Gibberella zeae PH-1
P38013    159 np-gtDVTVSSVESVLA 174 baker's yeast
Q9M7T0    185 ap--sDFKVTGAEVILG 199 thale cress
P56577    160 gd-tgKLQNASIDTILT 175 Pityriasis (Tinea) versicolor infection agent
XP_455979 150 np-atDVTVSSVDNALE 165 Kluyveromyces lactis NRRL Y-1140

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