2F3O


Conserved Protein Domain Family
PFL2-3

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TIGR01774: PFL2-3 
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glycyl radical enzyme, PFL2/glycerol dehydratase family
This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.
Statistics
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PSSM-Id: 273798
Aligned: 3 rows
Threshold Bit Score: 1357.66
Created: 8-Oct-2014
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2F3O_A   2 CMDRIEKLIKKVSKP--ARLSVERCRLYTESMKQTEGEPMIIRQAKALKHVLENIPIQILDSELIVGTMLPNPPGAIIFP 79  Archaeoglobus fulgidus
P32674   1 MTNRISRLKTALFANt-REISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSP 79  Escherichia coli K-12
P75793   9 LSDRIKAHKNALVHIvkPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFP 88  Escherichia coli K-12
2F3O_A  80 EGVGLRIINELDSLPNRETNRLMVDEEDAKVLReEIAPYWQRKTIEAFAFPLMPDIMQILYTGSVFVLT--EIAGISHVA 157 Archaeoglobus fulgidus
P32674  80 EMDPYWLLKELDQFPTRPQDRFAISEEDKRIYReELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINq-TDKGQGHII 158 Escherichia coli K-12
P75793  89 EYTVSWIEKEIDDLADRPGAGFAVSEENKRVLH-EVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEgnMTSGDAHLA 167 Escherichia coli K-12
2F3O_A 158 VNYPYLLRRGFRWFLEESERRIRaleesgvYeGEKYSFYQAAKIVSEAVINYGLRYSKLAEELAESEDGE-RREELLKIA 236 Archaeoglobus fulgidus
P32674 159 IDYPRLLNHGLGELVAQMQQHCQ-------Q-QPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAqRREELLTIA 230 Escherichia coli K-12
P75793 168 VNFPLLLEKGLDGLREEVAERRSrinltvlEdLHGEQFLKAIDIVLVAVSEHIERFAALAREMAATETREsRRDELLAMA 247 Escherichia coli K-12
2F3O_A 237 EICRKVPAEKPETFWEAVQFVWLVQSALHQENYEQAISMGRIDQYLYPFFKKDIGEGRi-NRELAFDILANLWIKTNEIV 315 Archaeoglobus fulgidus
P32674 231 EISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGE--DAAFLKELLESLWVKCNDIV 308 Escherichia coli K-12
P75793 248 ENCDLIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQtlDREHAIEMLHSCWLKLLEVN 327 Escherichia coli K-12
2F3O_A 316 PAFDSLLEQYFSGQATNQAVTIGGC---DIYGNDATNELTYLMLEVTDRLRLRQPNVHVRINKGSPESFLKRLAEAISSG 392 Archaeoglobus fulgidus
P32674 309 LLRSTSSARYFAGFPTGYTALLGGL---TENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRFG 385 Escherichia coli K-12
P75793 328 KIRSGSHSKASAGSPLYQNVTIGGQnlvDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCG 407 Escherichia coli K-12
2F3O_A 393 CNNLALFFDDAAVKALKNAEVDDRDALNYTTDGCVEIAPFGNS-FTSSDAALINVAKALEYALNEGVdlqfGYefgaKTe 471 Archaeoglobus fulgidus
P32674 386 TGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRT-YGLHDIAMFNLLKVMEICLHENE----GN----AA- 455 Escherichia coli K-12
P75793 408 FGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWgYRCTGMSFINFARVMLAALEGGHdatsGKvflpQEk 487 Escherichia coli K-12
2F3O_A 472 k-----pKFLEDLLEKLREQVSHIVKLVVRGSNVLSYANAEVKPTPLLSLCVEDCFEKGVDVSRGGARYNFTGIQAVGIA 546 Archaeoglobus fulgidus
P32674 456 -------LTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIA 528 Escherichia coli K-12
P75793 488 alsagnfNNFDEVMDAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIA 567 Escherichia coli K-12
2F3O_A 547 DVGDSLVAIEGALNAG--YSMDDIVEACRKNFVG---YEKLHKLLLQSPKYGNDDDAADKYTKMVLEWYCEEVNRHRNFR 621 Archaeoglobus fulgidus
P32674 529 NLSDSLHALKGMVFEQqrLSFDELLSVLKANFATpegEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPR 608 Escherichia coli K-12
P75793 568 NLGNSLAAVKKLVFEQgaIGQQQLAAALADDFDGlthEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPR 647 Escherichia coli K-12
2F3O_A 622 ------GGKFAAGCYPMTTNVGFGFFTSALPSGRKSGEPLNPG-VSPSTGMDREGVTAVINSASKLSYENLPNGASLTIN 694 Archaeoglobus fulgidus
P32674 609 ------GGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGgLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK 682 Escherichia coli K-12
P75793 648 ygrgpvGGNYYAGTSSISANVPFGAQTMATPDGRKAHTPLAEG-ASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQK 726 Escherichia coli K-12
2F3O_A 695 LSSDVLGEKGD--AVIEALIkSSMELGVMHVQFNILKEDLLRKAQQEPEKYRWLLVRVAGWSAYFVELSRPVQEEVIRRI 772 Archaeoglobus fulgidus
P32674 683 FTPATLEGEAGlrKLADFLR-AFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRT 761 Escherichia coli K-12
P75793 727 LNPATLENESDkqKLMILLRtFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIART 806 Escherichia coli K-12
2F3O_A 773 SCRI 776 Archaeoglobus fulgidus
P32674 762 AHQL 765 Escherichia coli K-12
P75793 807 EHML 810 Escherichia coli K-12
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