1EA0,1LLW


Conserved Protein Domain Family
GXGXG
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cd00504: GXGXG 
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GXGXG domain. This domain of unknown function is found at the C-terminus of the large subunit (gltB) of glutamate synthase (GltS), in subunit C of tungsten formylmethanofuran dehydrogenase (FwdC) and in subunit C of molybdenum formylmethanofuran dehydrogenase (FmdC). It is also found in a primarily archeal group of proteins predicted to encode part of the large subunit of GltS. It is characterized by a repeated GXXGXXXG motif. GltS is a complex iron-sulfur flavoprotein that catalyzes the synthesis of L-glutamate from L-glutamine and 2-oxoglutarate. It requires the transfer of ammonia and electrons among three distinct active centers that carry out L-Gln hydrolysis, conversion of 2-oxoglutarate into L-Glu, and electron uptake from a donor. These catalytic sites occur in other domains within the protein or or encoded by separate genes, and are not present in the domain in this CD. FwdC and FmdC are reversible ion pumps that catalyze the formylation and deformylation of methanofuran in hyperthermophiles and bacteria. They require the presence of either tungstun (FwdC) or molybdenum (FmdC). The specific function of this domain also remains unidentified in the formylmethanofuran dehydrogenases.
Links
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Statistics
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PSSM-Id: 238281
Aligned: 72 rows
Threshold Bit Score: 79.9206
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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domain_subunit
Conserved site includes 34 residues -Click on image for an interactive view with Cn3D
Feature 1:domain_subunit interface
Evidence:
  • Structure:1EAO_A: beta-helix domain interface with other domains, defined using 4.0 A contacts
  • Comment:Sharp inward bend along one face of the domain allows it to wedge itself into the helix core of the protein
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1            ##                         #  ##   #                         ###    #  #    
1EA0_A      1240 RAIGTRLssmvtrkfgmfglqpghITIRLRGTAGQSLGAFAv--------------qGIKLEVMGDANDYVGKGLSGGTI 1305 Azospirillum b...
O26340       115 MVSGTIYvkgavrd---pigniieVRSDRRGYKKFMSVTDIlmnglraepvnvtlepGRMVIDDGHVRDTLGARLDADVE 191  Methanothermob...
AAB89324      72 KWIGCRMt---------------dGEIVVKGSVGANCGAYMa---------------GGKIVIEGNADDWLGAEMKDGEI 121  Archaeoglobus ...
Q58571        75 KYIGSKMt---------------kGEIVVEGDAGMYVGAEMk---------------GGKIVVNGNAESWAGQNMKGGEL 124  Methanocaldoco...
Q48943       109 KRIGESMs---------------aGKIEIEGSAGMHVGTGMk---------------GGELVVYGDADSWAGMEMTGGLL 158  Methanosarcina...
NP_614813     77 KLIGYRMs---------------aGEILVKGDVGRHAGAEMk---------------GGKLIVEGDADDWLGREMKGGEI 126  Methanopyrus k...
O27002        73 KYIGSGMk---------------sGKIIINGDVGLQLGCEMk---------------GGEIEVNGNVSSWIGMEMHGGTI 122  Methanothermob...
NP_634002     77 KRIGQNMs---------------aGEIEINGNVDMHCGFAMk---------------GGKVVINGNADSWLGCEMSGGEI 126  Methanosarcina...
AAK30006      71 DFAGRGMt---------------rGSLIIDGDAGAYVGMHMk---------------GGRLCVSGSAGLYAACELKGGIV 120  Methylococcus ...
ZP_00034574   83 DRIGARMt---------------dGHLVVHGSTGDYSGFQMa---------------GGRLHIEGDAGHFTACEMRGGRL 132  Burkholderia f...

Feature 1                        ##  #  #  #           #####  ## #        ### #  ## #            
1EA0_A      1306 VVrpttsspletnkntIIGNTVLYGAT---AGKLFAAGQAGERFAVRNSGATVVVeGCGS-NGCEYMTGGTAVILGRVgd 1381 Azospirillum b...
O26340       192 II--------------HHGNVDLSTGIlmrRGTVRVMGDAGKNTGALLSGGTVIIqGDCDdFSGIDMRDGYLIVDGDAgk 257  Methanothermob...
AAB89324     122 IVkgnaa-----nlvgCAYYGDATGMS---GGKILIEGNAGNYIGEKMAGGEIEIkGNAGdFIGTEMKGGTIIIHGSCg- 192  Archaeoglobus ...
Q58571       125 LIkgnag-----dyvgSAYRGDWRGMS---GGTIIVEGNAGNEIGEFMSKGLIHIkGNVGiMAGIHQNGGIIIIDGDVdv 196  Methanocaldoco...
Q48943       159 HIkgnag-----dhvgCAYRGKWHGMK---GGRIVIEGSARHQLGGGMDGGEILVeGDVKsFCGIRQNGGLIFVKGSAlr 230  Methanosarcina...
NP_614813    127 TVhgnag-----nyvgSTYRGEWRGMS---GGRILVKGDAGDEIGEWMSDGKIIVeGDAGiMIGIHMQGGTIIVRGDVgv 198  Methanopyrus k...
O27002       123 KIngnag-----dyvgCAYRGEWRGMK---GGKIIIQGNAGNNIGGGMMAGEIYIgGDAGnFCGIRMNGGEITVRGDAgr 194  Methanothermob...
NP_634002    127 ILngnaa-----yyvgAGYRGESCGMR---GGKITVNGNAKDYLGEHMCGGEILVkGDVGlLPAISNNGGRIVIEGNAt- 197  Methanosarcina...
AAK30006     121 EIcgnag-----dllgSALPGNKKGMS---EGVVIVRGDAGDRVGDHMRRGSILIeGNAGmYLGARMTAGTIAVRGRIga 192  Methylococcus ...
ZP_00034574  133 TVtghsg-----dfaaGALAGDMEGMT---GGTLTIHGNAGARLADRMRRGLVLVgGNAGdFAASRLVAGTVGIAGQLga 204  Burkholderia f...

Feature 1        #  #  #        
1EA0_A      1382 NFAAGMTGGMAYVYD 1396 Azospirillum brasilense
O26340       258 FLGAQRRGGVILARR 272  Methanothermobacter thermautotrophicus str. Delta H
AAB89324     193 FVGGDMKAGEIKIGG 207  Archaeoglobus fulgidus DSM 4304
Q58571       197 RVGGEMKAGAIVVYG 211  Methanocaldococcus jannaschii
Q48943       231 GVGAEMAGGTIVIGG 245  Methanosarcina barkeri
NP_614813    199 RPGAQMEGGTVVVCG 213  Methanopyrus kandleri AV19
O27002       195 APGAEMVSGIIKIHG 209  Methanothermobacter thermautotrophicus
NP_634002    198 MPASEMKNGTVIIKG 212  Methanosarcina mazei Goe1
AAK30006     193 CAGYAMKRGTLLVYG 207  Methylococcus capsulatus
ZP_00034574  205 HYAYGMRRGTLLLAQ 219  Burkholderia fungorum

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