1QM6,1QM6,1OLP,1P6D


Conserved Protein Domain Family
Zn_dep_PLPC
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cd11009: Zn_dep_PLPC 
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Zinc dependent phospholipase C (alpha toxin)
This domain conveys a zinc dependent phospholipase C activity (EC 3.1.4.3). It is found in a monomeric phospholipase C of Bacillus cereus as well as in the alpha toxin of Clostridium perfringens and Clostridium bifermentans, which is involved in haemolysis and cell rupture. It is also found in a lecithinase of Listeria monocytogenes, which is involved in breaking the 2-membrane vacuoles that surround the bacterium.
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Statistics
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PSSM-Id: 211381
Aligned: 53 rows
Threshold Bit Score: 89.7459
Created: 18-Aug-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Zn binding site
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1: Zn binding site [ion binding site], 7 residue positions
Conserved feature residue pattern:H H H D H H EClick to see conserved feature residue pattern help
Evidence:
  • Comment:the zinc-binding site is the putative active site of this enzyme
  • Structure:1QM6: The phospholipase C domain of Clostridium perfringens alpha-toxin binds two zinc ions, contacts at 4 A
  • Structure:1OLP: The phospholipase C domain of alpha toxin from Clostridium absonum binds 3 zinc ions, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                     #                                                                  
1QM6_A         1 WDGKi---dGTGTHAMIVTQGVSILENdlskn------------------------------------------------ 29  Clostridium per...
NP_634924    121 MIDKe----AFREHTAVGVKWASSNHQ----------------------------------------------------- 143 Methanosarcina ...
YP_003480544 130 TWEPdh--hEDMTHPALADFEIAIGRRddiaestpqpd------------------------------------efgcee 171 Natrialba magad...
ZP_02000037   14 CLPGyilgyELETHATISQEATKLSVLgtn-------------------------------------------------- 43  Beggiatoa sp. PS
YP_002940140  12 LLAGti--fAWSGHDVLTYYILKSLNMdfsfevpitvysyenletreynlekasfddllgkdyipgednrlfkpvfensk 89  Thermotogales b...
ZP_07579340   11 LLTAfa--fSWSGHDALTYIIVSQYEDdfdafveitpytysdvdtqqynpaiegfydylgedylpeedldlyssifpnpk 88  Thermotogales b...
NP_229174     11 VVSAlv--fSWSGHEGLTYYIVKDIVPdemvtitpynyqenrvynlsklvled-------vcgqdtisawegailppnps 81  Thermotoga mari...
YP_004659751  12 ILICyv--fGWSAHETLTYLIVQSLPNkddlvpitpysyvesrvynmeylkled----ycgdfienfvpkwavffppdpk 85  Thermotoga ther...
YP_001470885  10 LITSiv--fSWSAHEVLTYLIVKDFLPnsdekiaitsysyqedrvyntdylkldd---ycgqfintfvpedaifyppdpk 84  Thermotoga lett...
YP_001409608  22 MTQAvi--fAWSGHEVYTYFLAKSLNApldklveitpysyketrlyntkyyytd----dfagqrkffdplnisefpedkp 95  Fervidobacteriu...

Feature 1                                                            #                           
1QM6_A        30 epesvRKNLEILKENMh----ELQLGSTYPDYDKnayd----------lyqDHFWDPdtdnnfskd-------------- 81  Clostridium per...
NP_634924    144 -----DFAYYACDGSAyg--nYASDTADDPDDPWfelpg--------yryyNHYWDAdw--------------------- 187 Methanosarcina ...
YP_003480544 172 csfdwLPGTGFLDDFLv---dAIAAAVNMHSDAHth-------------epYHFYWPdppsi------------------ 217 Natrialba magad...
ZP_02000037   44 --kkvLQNLGFTSKLDn---lFFPNGEGEVKKLLevigdgarfednwprffNHFYDPikklglygfwngaaspnwaidee 118 Beggiatoa sp. PS
YP_002940140  90 lengkAPIWQILSVYSyepdlGMDQNLELSNLQWmtgg---------sqgwRHMEYHlfw-------------------- 140 Thermotogales b...
ZP_07579340   89 pvdnlAPLWQILSVYSyepdlGMDKGLELKGIETllgd---------sqglRHMEYRmlf-------------------- 139 Thermotogales b...
NP_229174     82 pedgkVPAWQILTVYSsepdwGMDQGLDLSPYQFlign---------sqgvRHMKYRilg-------------------- 132 Thermotoga mari...
YP_004659751  86 pqdgkVPVWQILTIYSvepdlGMDEGLQLSPLQDligs---------skgvRHMKYRllv-------------------- 136 Thermotoga ther...
YP_001470885  85 pvnkeVFIWQVLTVYSvepdlGMDEGLNLSPLQSiign---------sqgvRHMKYKlli-------------------- 135 Thermotoga lett...
YP_001409608  96 pvdgkIPAFQILSIYAqfpdfGMDEGLNLSPMQAlign---------sqgvRHMRYKlgi-------------------- 146 Fervidobacteriu...

Feature 1                                                         #   #     #                    
1QM6_A        82 -nswylaysiPDTGESQIRKFSALARYEWQRg----NYKQATFYLGEAMHYFGDIDTPYHPAnvta-------------- 142 Clostridium per...
NP_634924    188 ---------hVGGAPTRCDGFADTAQIWVANg----LLEEAHEDFGLASHYLSDTGNPFHSAgavdqignf--------- 245 Methanosarcina ...
YP_003480544 218 ------dlylWEIGPSAFGGAPGEAQYMMDQay-dsSFSTRRDYLGYALHYVQDMGVPLHTGavwqqlnpdpqgcstsgc 290 Natrialba magad...
ZP_02000037  119 gslwpsspkdQDFSLKDGRDYLYQALTLPNAterekNFGLFFRTLGHVTHHIADMAQPQHTRndgh-------------- 184 Beggiatoa sp. PS
YP_002940140 141 --------lrFGEVSESVAYFTELSKKAWEKg----DPYWAFRFMGRAIHYFEDIGQPYHTFpapfcellklmi----dm 204 Thermotogales b...
ZP_07579340  140 -------firVGKVTDVVQYFSDLAEIAYSRg----DHYWAYRFLGRALHYLEDVGQPFHTFpapffellklpf----nm 204 Thermotogales b...
NP_229174    133 --------leFFEADKSFYYFHDMAKEAFRKg----DRYWGYRFLARALHYIEDLSQPYHNTpgevgeifqal-----fd 195 Thermotoga mari...
YP_004659751 137 --------vdFFEGSESVLYFINMSREAFKKg----DRYWGYRFLARALHYLQDLSMPYHNApgplfdtvrgi-----fd 199 Thermotoga ther...
YP_001470885 136 --------ldFFEGSQSFYYFVDMSRKAFKNg----DRYWGYRFLARALHYLEDLSMPFHNSpatfldtlkal-----sd 198 Thermotoga lett...
YP_001409608 147 --------ieAFEGDKSFLYFVDMSKKAFEKr----DEYWGYRFLSYALHYIEDLFQPYHESpgtfwevltsl-----fd 209 Fervidobacteriu...

Feature 1                  #   #                                                                 
1QM6_A       143 -----vdsaGHVKFETFAEEr--kEQYKINTAGcktneafytdilknkdfNAWSKEYARGFAKTGKSIYYSHAsms---- 211 Clostridium per...
NP_634924    246 -venlfntnNHDLYEIYISDqw-dSGYDFGDYVssnt---------qaitVTDPEQAVKDNADFSSQYFDYIWdeien-- 312 Methanosarcina ...
YP_003480544 291 dqyfdpkfdLHFDYEEWAADnfedADWYLDEPFeesfnd------sfpyhIPSVEEACKDLAEYTTQYATTVFeellttg 364 Natrialba magad...
ZP_02000037  185 ------afgDNVLYEGYTEKvvddKNRTLPYSGyap------------veFSQARNYWYTDTGQGLADYSNRGfv----- 241 Beggiatoa sp. PS
YP_002940140 205 nkwmtvftnYHLVYDYYSGY----LLWNQNAKVveaikt------akpikITDPRKDALRLRIYARRKLHKIYyemkkim 274 Thermotogales b...
ZP_07579340  205 dkwltvfanYHFAYDFYGGY----LLWEEYEPLveaike------vspkeIKDPKQAAVSLRRYSRNRLSSVYyelkrtm 274 Thermotogales b...
NP_229174    196 rsimkmlmnAHYVYDDYLAY----LIYSGDEETiktiee------atpvfLKSEWHLVQEVRKLALSYFPKVHreikkvf 265 Thermotoga mari...
YP_004659751 200 kdtalmlayTHFSYDEYMAY----LLYRNDEETinailh------aepkkVRNTRELIQRVRLLGLRNISKVHrlmthhf 269 Thermotoga ther...
YP_001470885 199 kqmaqfltnLHYSYDDYLAY----LLYNADEKTveaiis------apvekSRSLRQLIQKVRLLGLNNINTVSnemkrnf 268 Thermotoga lett...
YP_001409608 210 kkvmkmlnnAHYTYDNYLVY----LLVYSKNREevvkiie---ktparyvSKNYEKLINEVMMYSYSKFPQVHselkkaf 282 Fervidobacteriu...

Feature 1                                                                   
1QM6_A       212 ----------------------------hswDDWDYAAKVTLANSQKGTAGYIYRFLHD 242 Clostridium perfringens
NP_634924    313 ---------------------------npdtFDSDIYVAYYTAQCVRNTAKCNHGLYDY 344 Methanosarcina mazei Go1
YP_003480544 365 s-----------------------dnpslwdSKIYELTHNCMYETGGYTRGLITQFYDI 400 Natrialba magadii ATCC 43099
ZP_02000037  242 -----------------------------saDTNFDTSYYADPKPLRDNNGQIKTIKKS 271 Beggiatoa sp. PS
YP_002940140 275 kddlevssef-----mpspayfdlltvegktKKLEELTADLLGKVASYIKGYLIYMNEE 328 Thermotogales bacterium TBF 19.5.1
ZP_07579340  275 gdklenpesa-----wpgkayfddlqdagetAKLDALSVEILRETASYVKGYINYMLAR 328 Thermotogales bacterium mesG1.Ag.4.2
NP_229174    266 geklkertsdlesftvisveefreadqrgdlVLLKRYTLELIGKVSSYLKGYLIDFLKA 324 Thermotoga maritima MSB8
YP_004659751 270 gedlkgr----------ilgledferkstelQELKQITISIARDLSSLLKGFLLDYLKE 318 Thermotoga thermarum DSM 5069
YP_001470885 269 eeelkvr----------ilnfddfkfrkdelYTLKQTTVEIISRFSSIARGFLIDFLKE 317 Thermotoga lettingae TMO
YP_001409608 283 gdmlyeri--------peiedfkkleeegklDKLYSVTKDIIVVMTGTIKGFLEEYLKS 333 Fervidobacterium nodosum Rt17-B1

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