This group is comprised of eukaryotic and bacterial proteins similar to Escherichia coli RsuA. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. E.coli RsuA makes psi516 in 16S RNA. Psi at this position is not generally conserved in other organisms.
Structure:1KSL_A, Escherichia coli RsuA bound with uracil, contacts at 3.5 A
Comment:UMP binds in the same region as uracil but, makes different interactions with the protein. Neither UMP nor uracil is a substrate for pseudouridines but they recognize the position occupied by the target uracil base in an RNA molecule.
Jiyao W et al.(2023). "The conserved domain database in 2023.",