Conserved Protein Domain Family
T4SS-DNA_transf

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pfam02534: T4SS-DNA_transf 
Type IV secretory system Conjugative DNA transfer
These proteins contain a P-loop and walker-B site for nucleotide binding. TraG is essential for DNA transfer in bacterial conjugation. These proteins are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems. The C-terminus of this domain interacts with the relaxosome component TraM via the latter's tetramerization domain. TraD is a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore. The family contains a number of different DNA transfer proteins.
Statistics
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PSSM-Id: 367119
Aligned: 9 rows
Threshold Bit Score: 517.343
Created: 21-Aug-2024
Updated: 4-Dec-2024
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
O25260       177 NKKVDDLFGSAS-WETEEKMIKAKLITPNNKk-----------rAFDKREvivgRRGLGDFiay--agqAFIGLIAPTRS 242 Helicobacter py...
P13464        97 RWAAFGEMRHAG-YLQRYNRIKGPVFGKTCG-------------PLWFGN----YLTNGEQ--------PHSLVVAPTRA 150 Rhizobium rhizo...
O32582       101 KFASDSDLKKSK-LLKWEKENDTDILVGR-----------------YKGKy--lWYTAPDF----------VSLGAGTRA 150 Escherichia coli
O87219         2 NGTILGVLDNK--IIYQDNTTKP--------------------------------NRN-------------VMVIGGSGS 34  Lactococcus lactis
1_pfamImport   1 MTTLLGELESKAtSINKKNLVIQDFDTKCP-------------------------NRN-------------IFVVGGPGS 42 
Q9ZDN4       120 SWANQSDIEAAG-LRSKKGMLIG----VD------------------AGGy---FVADG-F--------QHALLFAPTGS 164 Rickettsia prow...
Q44346       163 DWMKIQEAAKL--FPERGGIIIGERYRVDRDsvaampfraddrqSWGAGGksplLCFDGSFg------sSHGIVFAGSGG 234 Agrobacterium f...
P55421       163 DWMNMQDAARL--FSDAGGVVIGERYRVDKDstaarsfraddpeTWGAGGksplLCFDGSFg------sSHGIVFAGSGG 234 Sinorhizobium f...
WP_011205817 120 RWADKKDIQAAG-LLPRPRTVVELVSGKHPPts----------sGVYVGGw---QDKDGKFhylrhngpEHVLTYAPTRS 185 Pseudomonadota
O25260       243 GKGVGFIMPNMINYPQ-NIVVFDPKADTMETCGKIREKRFNqKVFIYEPFSLKTH--RFNPFAYVDFGNDv-VLTEDILS 318 Helicobacter py...
P13464       151 GKGVGVVIPTLLTFKG-TVIALDVKGELFELTSRARKSSGD-AVFKFSPLDPERRthCYNPVLDIAALPPerQFTETRRL 228 Rhizobium rhizo...
O32582       151 GKGAAIGIPNMLVKKH-SIIALDPKQELWKITSKVREQILGnKVYLLDPFNTKTH--KCNPLFYVDLKSE--SGAKDLLK 225 Escherichia coli
O87219        35 YKTQSVVITNLFNETKnSIVVTDPKGELYEKTAGIKLAQGY-EVHVVNFANMAHSd-RYNPFDYIERDIQ--AESVATKI 110 Lactococcus lactis
1_pfamImport  43 YKSAGYVIPNVIVKNQqSIVVTDPSGEVYEKTSNIKRMQGF-DVRVVNFKNFLASd-RQNFFDYIDKDSD--CSIVAELI 118
Q9ZDN4       165 GKGVGFVIPNLLFWTD-SVVVHDIKLENHNLTSGWREKQGQ-KVFVWEPSNPDGIthCYNPIDWVSTKPG--QMVDDVQK 240 Rickettsia prow...
Q44346       235 FKTTSVTIPTALKWGG-GLVVLDPSSEVAPMVCEHRRQAGR-KVIVLDPTAGGVG---FNALDWIGRHGN--TKEEDIVA 307 Agrobacterium f...
P55421       235 FKTTSVTIPTALKWGG-ALVVLDPSNEVAPMVYEHRKAAAR-FIRILDPKEPETG---FNALDWIGRFGG--TKEEDIAS 307 Sinorhizobium f...
WP_011205817 186 GKGVGLVVPTLLSWAH-SAVITDLKGELWALTAGWRKKHARnKVVRFEPASAQGSa-CWNPLDEIRLGTE--YEVGDVQN 261 Pseudomonadota
O25260       319 QIDTRLKGHGMvasggdfstqifglaklvfperpnekDPFFSNQARNLFVIncniyrdlmwtkkgLEFVKRKKIIMPETp 398 Helicobacter py...
P13464       229 AANLITAKGKG--------------------------AEGFIDGARDLFVA--------------GILTCIERGTPTIG- 267 Rhizobium rhizo...
O32582       226 LVEILFPSFGLtg-----------------------aEAHFNNLAGQYWTGlakllhffinfapeWIEELRVKPVFSIGt 282 Escherichia coli
O87219       111 VQSENAEGK----------------------------KDVWFSTQRQLLKAli-----------lFVMKERSPEQRNLAg 151 Lactococcus lactis
1_pfamImport 119 IKSAGDSKIN---------------------------KDVWYKASVGLLNSll-----------lYAKYEFEPEKRTIGn 160
Q9ZDN4       241 ISNLIMPEK-----------------------------DFWNNEARSLFLG--------------VTLYLIADPTKTKSf 277 Rickettsia prow...
Q44346       308 VATWIMTDNPRtas---------------------arDDFFRASAMQLLTAl-------------IADVCLSGHTETEDq 353 Agrobacterium f...
P55421       308 VASWIMSDSGGmrg---------------------vrDDFFRASALQLLTAl-------------IADVCLSGHTDKENq 353 Sinorhizobium f...
WP_011205817 262 LATLIVDPDGKg------------------------lESHWQKTSQALLVGv-------------ILHALYKAKNEGTPa 304 Pseudomonadota
O25260       399 tMFFIGSMASGInlidedTNMEKVVSLMEFFGGEEDKSGDNLRVLSPATRnmwnsfktmggarETYSSVQGvyTSAFAPY 478 Helicobacter py...
P13464       268 -AVYDLFAQPGE------KYKLFAHLAEESRNKEAQRIFDNMAGNDTKIL-------------TSYTSVLG--DGGLNLW 325 Rhizobium rhizo...
O32582       283 vVDLYSNIDREQvls-nrETFEELAQGNETALFHLKDALTKIREYHETED-------------EQRSSIDGsfRKKMSLF 348 Escherichia coli
O87219       152 vINVLQTFDSEPin--kdENSDLDNLFLALKITHPARIAYELGFKKAKGD--------------MKASIISslLATISKF 215 Lactococcus lactis
1_pfamImport 161 iIKFIQNNKPNQde---eGSVELDKRFNELSKDHPARESYEFGFAVSEGR--------------TRASIILtfVADLRNY 223
Q9ZDN4       278 -GEVVRTMRSDD------VVYNLAVVLDTLGGVIHPVAYMNIAAFLQKAD-------------KERSGVIStmNSSLELW 337 Rickettsia prow...
Q44346       354 tLRRVRANLSEP------EPKLRARLTKIYEGSESDFVKENVSVFVNMTP-------------ETFSGVYAnaVKETHWL 414 Agrobacterium f...
P55421       354 tLRQVRANLAEP------EPKLRERLQSIYDNSNSDFVKENVAAYVNMTP-------------ETFSGVYAnaVKETHWL 414 Sinorhizobium f...
WP_011205817 305 tLPSVDGMLADPnrd-vgELWMEMTTYGHVDGQNHPAVGSAARDMMDRPE-------------EESGSVLStaKSYLALY 370 Pseudomonadota
O25260       479 NNAMIRNFTSANDFDFRRLR--IDEVSIGVIANPKESTIVGPILELFFN-VMIYSNLILPIHDPQcKRs----CLMLMDE 551 Helicobacter py...
P13464       326 ADPLVKAATSRSDFSVYDLR--RKRTCVYLCVSPNDLEVVAPLMRLLFQqVVSILQRSLPGKDER-YE-----VLFLLDE 397 Rhizobium rhizo...
O32582       349 YLPTVRNALT-YDFDLRQMR--REDITVYVGVNAEDMILAYDFLNLFFNlVVEVTLRENPDFDPTlKHd----CLLFLDE 421 Escherichia coli
O87219       216 TDEEVSNFTSISDFHLQDIG--RKKIVLYVIIPVMDNTYESFINLFFSQmFDELYKLASSNGAKLpQE-----VDFILDE 288 Lactococcus lactis
1_pfamImport 224 IDNEIRSYTSDSDFDLRDVG--LRKTIIYVMLPVLGNTVQSLSSLFFSQmFQQLYRLGDENGARLpVP-----VDFLLDE 296
Q9ZDN4       338 ANPLIDSATASSDFNIQEFK--KVKTTVYVGLTPDNIQRLQKLMQVFYQqATEFLSRKMPDLKEEpYG-----VMFLLDE 410 Rickettsia prow...
Q44346       415 SYPNYAGLVSGDSFSTDDLA--DGGTDIFIALDLKVLEAHPGLARVVIGsLLNAIYNRNGNVKGR--------TLFLLDE 484 Agrobacterium f...
P55421       415 SYPNYAALVSGSTFSTDALA--AGETDVFINIDLKTLETHAGLARVIIGsFLNAIYNRDGEVKGR--------ALFFLDE 484 Sinorhizobium f...
WP_011205817 371 RDPVVARNVSKSDFRIKQLMhhDDPVSLFIVTQPNDKARLRPLVRVMVNmIVRLLADKMDFENGRpVAhykhrLLMMLDE 450 Pseudomonadota
O25260       552 FTLCGYLETFVKAVGIMAEYNMRPAFVFQSKAQLENdpplGYGRNGAKTILDNlSLNMYYGINNDnyYEHFEKLSKVLGK 631 Helicobacter py...
P13464       398 FKHLGKLEAIETAITTIAGYKGRFMFIIQSLSALSG----TYDEAGKQNFLSN-TGVQVFMATAD--DETPTYISKAIGE 470 Rhizobium rhizo...
O32582       422 FPSIGYMPIIKKGSGYIAGYKLKLLTIYQNISQLNE----IYGTEGAKTLLSAhPCRVIYAVSE---EDDAKKISEKLGY 494 Escherichia coli
O87219       289 FVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQS----LYGKEKAESILGNhAVKICLNASN---EATAKYFSELLGK 361 Lactococcus lactis
1_pfamImport 297 WPNIGAIPDYEETLATCRKYGISITTIVQSISQAVD----KYNKDKANAIIGNhAVILCLGNVN---NDTAKYIKEELGN 369
Q9ZDN4       411 FPTLGKMDTFKAGIAYFRGYRVRLFLIIQDTQQLKG----TYEDAGMNSFLSNsTYRITFAANN---YETANLISQLVGN 483 Rickettsia prow...
Q44346       485 VARLGYLRILETARDAGRKYGIMLTMIFQSLGQMRE----AYGGRDATSKWFEsASWISFAAIND--PDTADYISKRCGD 558 Agrobacterium f...
P55421       485 VARLGYMRILETARDAGRKYGIMLTMIYQSIGQMRE----TYGGRDAASKWFEsASWISFAAIND--PETADYISRRCGM 558 Sinorhizobium f...
WP_011205817 451 FPSLGKLEILQESLAFVAGYGIKCYLICQDINQLKS----RETGYGHDESITSnCHVQNAYPPNR--VETAEHLSKLTGT 524 Pseudomonadota
O25260       632 YTRQDVSRSI-----DDNTGKTNT-SISNKERFLMTPDELMTMGDELI------------I-LENTLKPIKCHKALYYDD 692 Helicobacter py...
P13464       471 YTFQARSTSY-----SQARMFDHNiQISDQGAPLLRPEQVRLLDDKSE------------IvLIKGQPPLKLRKVRYYSD 533 Rhizobium rhizo...
O32582       495 ITARSKGSSK-----TSGKATSRSnSENEAQRALVLPQELGTLDFSEE------------MiILKGEHPVKAEKALYYLD 557 Escherichia coli
O87219       362 STVKVETGSEst-shSKETSTSKSdSYSYTSRQLMTPDEIIRMPDTQS------------LlIFTNQKPIKATKAFQFKL 428 Lactococcus lactis
1_pfamImport 370 ATVEFETSSEgsssgKDSRSKSSNkNVSYTGRALLNEDEISNMQQDKA------------IlITKNRNPKIIKKLAYFKM 437
Q9ZDN4       484 KTVEQRSFSKpl--fFDLNISTRTqNVSQVQRALLLPQEVIQLPRDEQ------------IvLIESFPPIKSRKIKYYED 549 Rickettsia prow...
Q44346       559 TTVEVDQTNRs----TGMKGSSRSrSKQLSRRPLILPHEVLHMRSDEQ------------IvFTSGNPPLRCGRAIWFRR 622 Agrobacterium f...
P55421       559 TTVEIDQVSRs----FQTKGSSRTrSKQLAARPLIQPHEVLRMRADEQ------------IvFTAGNAPLRCGRAIWFRR 622 Sinorhizobium f...
WP_011205817 525 TTIVKEQITTsg-rrTSALLGNVSrTFQEVQRPLLTPDECLRMPGPKKsadgsieeagdmVvYVAGYPAIYGKQPLYFKD 603 Pseudomonadota
O25260       693 ---------------------------PFFTDELIKVSpSLSKKYKLG 713 Helicobacter pylori
P13464       534 rmlrrlfecqigalpepaslmlaqdvhQDGQDHLSQQA-AVTAALGLG 580 Rhizobium rhizogenes
O32582       558 ny-----------------------fmERLMLVSPKLT-ALTASINRS 581 Escherichia coli
O87219       429 fpd----------------------adSKVKLEQNKYVgITSKSQLEK 454 Lactococcus lactis
1_pfamImport 438 fpn----------------------ltETYIAPQNEYKrKKNQSMIDK 463
Q9ZDN4       550 ---------------------------KFFTSRLLPPT-FVPTQVPFN 569 Rickettsia prowazekii
Q44346       623 ---------------------------DDMKACVGENR-FH--RTGTG 640 Agrobacterium fabrum str. C58
P55421       623 ---------------------------EDMKACGGEP-------VSQG 636 Sinorhizobium fredii NGR234
WP_011205817 604 ---------------------------PIFQARAAVP------APKVS 618 Pseudomonadota
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