1F1S,1N7R,1X1J,1RW9,1HMW,1HN0,1J0M,1J0N,1RWH,1LXM,1LXK,1OJM


Conserved Protein Domain Family
GAG_Lyase
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cd01083: GAG_Lyase 
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Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
Links
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Statistics
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PSSM-Id: 238517
Aligned: 39 rows
Threshold Bit Score: 436.824
Created: 25-Apr-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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substratecatalytic
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site [chemical binding site]
Evidence:
  • Structure:1N7R_A; Streptococcus Pneumoniae Hyaluronate Lyase binds Hyaluronan; contacts calculated at 3.5A
  • Structure:1XIJ_A; Bacillus Xanthan Lyase binds substrate 46D; contacts calculated at 3.5A
  • Structure:1RWH_A; Arthrobacter aurescens Chondroitin Ac Lyase binds Chondroitin Tetrasaccharide; contacts calculated at 3.5A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                               
1F1S_A               81 NFTKLLDKWNDVtignyvydtnds-------------nmqklnqkldeTNAKNIEAIKldsnrtflw------------- 134  Strepto...
1N7R_A                4 TYTDRLDDWNGIiagnqyydsknd-------------qmaklnqelegKVADSLSSISsqadriylw------------- 57   Strepto...
1HN0_A              256 AIDLIRQRLINEfvggeketn-------------------laleenisKLKSDFDALNihtlanggtqgrhlitdkqiii 316  Proteus...
AAX07421             21 DFDKMLDNIKSEytqnptkkn-------------------idafrillKTDGSFSDIDytk------------------- 62   Flavoba...
AAZ36518            223 VFTRLCDQIPKSips-------------------------------aqDIDAWLSGFSdgkfrdityp------------ 259  Pseudom...
BAC97662             36 DFAQQASRNADNkgqtlsglat----------------qyrdalladgSWPDIDYTIVat-------------------- 79   Vibrio ...
ZP_00797056         281 NREKLRAHFAALkiqrd--------------------------adgqlSGRHIITDKQkvlyqpefmd------------ 322  Yersini...
REF_mbpwusl:BT3350  219 TIEQRLTEFLDVkkapq---------------------------gqinAARKTFERAAiapsaagtgftgtpv------- 264  Bactero...
CAG22026            222 MYDQMLKAENARfnfraefsdtqgdaaplyrqfdqhltvneqqtisqeKIDANLAKYTpfnirynaddsvtg-------- 293  Photoba...
AAO78430            238 EAETIRKKYDLYqitykdgqvsgvp-----------ifmvraseayerMIPDWDKDMLtk-------------------- 286  Bactero...

Feature 1                                       #  #   #                                                
1F1S_A              135 --------kdldnlnnsAQLTATYRRLEDLAKQITnphst--------------iykneKAIRTVKESLAWLHQNfynvn 192  Strepto...
1N7R_A               58 --------ekfsnyktsANLTATYRKLEEMAKQVTnpssr--------------yyqdeTVVRTVRDSMEWMHKHvynse 115  Strepto...
1HN0_A              317 yqpenlnsqdkqlfdnyVILGNYTTLMFNISRAYVlekdp---------------tqkaQLKQMYLLMTKHLLDQgfvk- 380  Proteus...
AAX07421             63 ---------------kdKDLRSHLSRISRLAQAYSnssnt--------------yyqdqSVYNDYVKSIEFWITTnht-- 111  Flavoba...
AAZ36518            260 --------aqgstvediSGLNLHLDRAFRIACYLFsqat----------------aspaHYLSHALEALNFYAGQdy--- 312  Pseudom...
BAC97662             80 ---------------deKPIRAHLDRIRVLAAAEHlfp-------------------qtGYAQAAIEAMYYWYSAdrt-- 123  Vibrio ...
ZP_00797056         323 -------didqqrfsdyVLLGDYTTLMFNISRAYQrstse---------------evrqELADMYILMTQHLLDQgymd- 379  Yersini...
REF_mbpwusl:BT3350  265 ---vapdeqdkkkgemsWNDIETMLAGFAYDAFYNqn---------------------eTSKKNYFTVFDYAMDQgfaf- 319  Bactero...
CAG22026            294 ---epldhpkrhnflkaGIVGDATLAMLTDTMAIRtlgktmletakflrthslsavnrdSLEQVFINATRYALNQgweg- 369  Photoba...
AAO78430            287 ---------------mgIEMRAYFDLMKRIAVAYNnseag--------------spvreEMKRKFLAMYDHITDQgvay- 336  Bactero...

Feature 1                      # ##   #  #   #                                                #     #   
1F1S_A              193 kdiegsaNWWDFEIGVPRSITGTLSLMNny---ftDAEIKTYTDPIEhfvpdaeyfrk-tlvnpfkalgGNLVDMGRVKI 268  Strepto...
1N7R_A              116 --ksivgNAADYEIGTPRAINNTLSLMKey---fsDEEIKKYTDVIEkfvpdpehfrk-ttdnpvkalgGNLVDMGRVKV 189  Strepto...
1HN0_A              381 ---gsalVTTHHWGYSSRWWYISTLLMSdal--keANLQTQVYDSLLwysrefkssfd-mkvsadssdlDYFNTLSRQHL 454  Proteus...
AAX07421            112 -----ptNWWYRHIAYPKEMNKGLVFVIeeiktknPTLYRKIIDYQEwaylqq-----------dhmegANGADKTIGAF 175  Flavoba...
AAZ36518            313 -----kiSWWNRQIGLAKKAGRTAVLLAkhl--tgSELIKQFIPYAMkttnty----------vytqtgANLADFASVQI 375  Pseudom...
BAC97662            124 -----nkNWWWNEIGKQLRLGPAALMLGsa----lPSDLKTLIQGDMpsap--------------yktgANRTDLSKAVI 180  Vibrio ...
ZP_00797056         380 ---gsglVTTHHWGYSSRWWYISAMLMAnel--dkHQLRQPVSDALLwysrefkanfd-mvpgpessdlDYFNTLSRQHL 453  Yersini...
REF_mbpwusl:BT3350  320 ---gsgmGTNHHYGYQVRKIYTTAWLMRdai--ykHPHRDAYLSTLRfwaalqetrq--pcpperdellDSWHTLLMAKF 392  Bactero...
CAG22026            370 ---gsgfQIITHVGYQTREYFDAMFIARqll--adNQLLEPVQQSMMwfnatgrvyeaddqinssnvdiLNTQLQWMVKS 444  Photoba...
AAO78430            337 ---gscwGNIHHYGYSVRGLYPAYFLMKdvl--reEGKLLEAERTLRwyaitnevypk-pegngidmdsFNTQTTGRIAS 410  Bactero...

Feature 1                                                                   #                #  #  #   #
1F1S_A              269 IEGLlr-------kDNTIIEKTSHSLKNlftt-----atkAEGFYADGSYIDHTNva--------ytGAYGNVLIDGLTQ 328  Strepto...
1N7R_A              190 IAGLlr-------kDDQEISSTIRSIEQvfkl-----vdqGEGFYQDGSYIDHTNva--------ytGAYGNVLIDGLSQ 249  Strepto...
1HN0_A              455 ALLLlepd---dqkRINLVNTFSHYITGaltqv---ppggKDGLRPDGTAWRHEGn----------yPGYSFPAFKNASQ 518  Proteus...
AAX07421            176 VAAVae-------kDANLLKQFSDLMKRltsi-----qegGEGIEKDYGFYSHSGngrq-----iytFGYGKEYLKSVLD 238  Flavoba...
AAZ36518            376 LWSVsawknsgqgsYLLYLRAAADVLSGlcqpvkregkehGEGVSVDYAINQHNAlngsqycmqlysGSYGAELLNRIVE 455  Pseudom...
BAC97662            181 FGGLla-------nDAAQVQRGLEGIAEtiv------iteAEGVQADYSFQQHGAql--------ytGGYGEVFFDTASF 239  Vibrio ...
ZP_00797056         454 ALLMlesd---pakRVALFKRFGEYINValsqt---ppggYDGLRPDGTAWRHEGn----------yPGYSFPAFKNAGQ 517  Yersini...
REF_mbpwusl:BT3350  393 ISAMmf-------pDAREQEQALNGLSRwlssslnytpgtLGGIKVDGTTFHHGGf----------yPGYTTGVLATIGQ 455  Bactero...
CAG22026            445 FLLLpdq-----seRDMMLKQLQSWLSKtlla----sdglSGGFKPDGSVFHHSQh----------yPAYGKDAFNGLSG 505  Photoba...
AAO78430            411 ILMMedt-----peKLQYLKSFSRWIDYgcrp----apglAGSFKVDGGAFHHRNn----------yPAYAVGGLDGATN 471  Bactero...

Feature 1                                                          ##  #                                
1F1S_A              329 LLPIIQetdykisnqeLDMVYKWINQsflpLIVKGELMDMSRGRSISReaass--haaaVEVLRGFLRLAnmsn------ 400  Strepto...
1N7R_A              250 LLPVIQktknpidkdkMQTMYHWIDKsfapLLVNGELMDMSRGRSISRanseg--hvaaVEVLRGIHRIAdmse------ 321  Strepto...
1HN0_A              519 LIYLLRdtpfsvgesgWNNLKKAMVSa--wIYSNPEVGLPLAGRHPFNsp-------slKSVAQGYYWLAmsak------ 583  Proteus...
AAX07421            239 YFVFTKgtqy--nvqtLVNLEKMVIDhvqyLFHAGNYDPNPTGRYNNTfeym----ddlKNIVTKMVALNta-------- 304  Flavoba...
AAZ36518            456 GAVVLVsefsl-tataLSELVNVVVEgmgwMGYASRMDFHVNGRAISRgvpsnahiaksAEVLLPFADTAn--------- 525  Pseudom...
BAC97662            240 WAYHVRdlqwkfspeqIDLLSDYFLEgvrwMNSHGTLDYNARGRGISRvqvvd--kstlQQQSLYIAALSpq-------- 309  Vibrio ...
ZP_00797056         518 LVYMLHgtpfavsdegRAALKKAMLSa--wVYSNPATSLGLAGRHPFNsssv----tlfQDAFRWLALTGdpktgd---- 587  Yersini...
REF_mbpwusl:BT3350  456 FIALTNgtgfelteeaRQHIKSAFLAmr-nYCNLYEWGTGISGRHPFGgkmg----sddIEAFANIALSGdlsgqgn--- 527  Bactero...
CAG22026            506 AVFGLSgstyqlsqaaHERIKDVLLKmr-vYTKETHTPIVLSGRHPDGkqk------vsATPFKWLALSGspdstq---- 574  Photoba...
AAO78430            472 MIYLFSrtslavselaHRTVKDVLLAmr-fYCNKLNFPLSMSGRHPDGqg-------klVPMHYAMMAIAgtpdgkgdfd 543  Bactero...

Feature 1                                                                                               
1F1S_A              401 --------------------------eerNLDLKSTIKTIITSnkfynvfnnlksysdianmnkllndstvatkplkSNL 454  Strepto...
1N7R_A              322 --------------------------getKQRLQSLVKTIVQSdsyydvfknlktykdislmqsllsdagvasvprtSYL 375  Strepto...
1HN0_A              584 --------------------------sspDKTLASIYLAISDKtqnestaifg---------------etitpaslpQGF 622  Proteus...
AAX07421            305 ----------------------------nKSALQDAHDRMSGQkk------------------------------dlEGN 326  Flavoba...
AAZ36518            526 -----------------------------KEALNELIRRTSGDesnn---------------------------qyyRGE 549  Pseudom...
BAC97662            310 ----------------------------rAQEAEQFRRHVAGEga------------------------------gfEGF 331  Vibrio ...
ZP_00797056         588 -----------------------kvdkalAAAYLQITETPESEseaifgvr-------------------iapaelpQGN 625  Yersini...
REF_mbpwusl:BT3350  528 -----------------------tfdhglAADYLRLIRDRNTRnahffrke------------------giqpaqapHGF 566  Bactero...
CAG22026            575 -----------------------ptdpdlAAAYANLVKKPSFEgvta--------------------------eaepTGV 605  Photoba...
AAO78430            544 kemasaylrlvssdsssaeqapeympkvsNAQERKIAKRLVENgfra--------------------------esdpQGN 597  Bactero...

Feature 1                                                      ##                                       
1F1S_A              455 STFN--SMDRLAYYNak---kDFGFALSLHSKRTLNYEGMNDENTRGWYTGDGMFYIYnsdqSHYSn-----hFWPTVNP 524  Strepto...
1N7R_A              376 SAFN--KMDKTAMYNae---kGFGFGLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYngdlSHYSd-----gYWPTVNP 445  Strepto...
1HN0_A              623 YAFN--GGAFGIHRWq-----DKMVTLKAYNTNVWSSEIYNKDNRYGRYQSHGVAQIVsn-gSQLSqg----yQQEGWDW 690  Proteus...
AAX07421            327 KMFW--RGDYMAHKRs-----NFLSTVRMTSTRTVGAEAGNNSGNYNYYAGAGVNYTIht-gNEYNg-----tYFSKFNP 393  Flavoba...
AAZ36518            550 RLFW--VNDYLAHIGs-----HYCVWAKAISTRTVGGESGNGENPKGYYMGAGTCFLThh-gKEYEg------IQPVWDW 615  Pseudom...
BAC97662            332 KQFW--RSDYASKVGe-----NHFIGVKMNSLRIEPTEAGNNENLLGNWLGFGSMFIMqr-gDEYHn------LFPVWNW 397  Vibrio ...
ZP_00797056         626 WTFN--GGAFGIHRFs-----DKMVTLKAYNSNVWSSEIYYRDNRYGRYQSHGAIQVLpy-gNQKEig----fVQDGWDW 693  Yersini...
REF_mbpwusl:BT3350  567 FVYN--YGSAGIFRRa-----DWMVTLKGYTTDVWGAEIYAKDNRYGRYQSYGSVQIMgk-gNPVSrag-sgfVQEGWDW 637  Bactero...
CAG22026            606 WAMNyaSMAIARGQSptkptqSWLATARGFSRYLVGNETYQANNLYGRYLQYGQLEITp---SDFSkq---afSHDGWDW 679  Photoba...
AAO78430            598 LSLG--YGCVSVQRRe-----NWSAVARGHSRYLWAAEHYLGHNLYGRYLAHGSLQILtappGQTVtpatsgwQQEGFDW 670  Bactero...

Feature 1                                                                                               
1F1S_A              525 YKMAGTTEKDAkredttkefmskhskdakektgQVTGTSDFVGSVKLndHFALAAMDFTnw------drTLTAQKGWVIL 598  Strepto...
1N7R_A              446 YKMPGTTETDAkrad---------------sdtGKVLPSAFVGTSKLddANATATMDFTnw------nqTLTAHKSWFML 504  Strepto...
1HN0_A              691 NRMEGATTIHLplkdlds---------pkphtlMQRGERGFSGTSSLegQYGMMAFNLIypanlerfdpNFTAKKSVLAA 761  Proteus...
AAX07421            394 RQFPGTTVEQNtitlpv-----------pnwgkEGKNNNDFAGGVSDg-KNGASAMIYDk--------qGITANKSWFFI 453  Flavoba...
AAZ36518            616 QRLPGTTVEQVpnfkwp----------ntawgvNMWGSHDFAGGVSDg-KRTLLSMELSrk-------nVTHAYKTVMAT 677  Pseudom...
BAC97662            398 ALVPGVTAPQFaekp--------------adwgSIVMNTSFVGGVSDg-RYGVAVMDMNa--------yGTQAKKAWFSF 454  Vibrio ...
ZP_00797056         694 NRNPGTTTIHLplaelds---------pnthtlMLRGNHPFSGHSSLsgKYGMFAFKFDapsmp-kfdsSFTARKSALET 763  Yersini...
REF_mbpwusl:BT3350  638 NRLPGTTTIHLpfellds---------plkgttMARSTENFSGSSSLggMNGMFAMKLTerdye-nftpDFVARKSVFCF 707  Bactero...
CAG22026            680 NRYPGTTTIHLpnnelktvln---qlpgagieeMLLSTETYSGANTLnnDSAMFAMKLHghkk--ygqqSLRARKSYFMF 754  Photoba...
AAO78430            671 NRIPGVTSIHLpldllkanvl--nvdtfsgmeeMLYSDEAFAGGLSQgkMNGNFGMKLHehdk---yngTHRARKSYHFI 745  Bactero...

Feature 1                                                                                               
1F1S_A              599 NDKIVFLGSNIKNtngigNVSTTIDQRKddsktPYTTYVNgktidlkqa--ssqqftdtKSVFLEskepgrnIGYIFFKN 676  Strepto...
1N7R_A              505 KDKIAFLGSNIQNts-tdTAATTIDQRKlessnPYKVYVNdkeaslte---qekdypetQSVFLEssdskknIGYFFFKK 580  Strepto...
1HN0_A              762 DNHLIFIGSNINSsdknkNVETTLFQHAit-ptLNTLWINgqkienmp---yqttlqqgDWLIDSn-----gNGYLITQA 832  Proteus...
AAX07421            454 GNLVLALGSEISQtngtsDVYTTLNQTNl---dASKLSYFqn----------------gTRFQLN-------ANANMTNN 507  Flavoba...
AAZ36518            678 NDRVTCMGTGIDTrsvmfPVVTCVNQCIa----RGPVRYLtidnqehtleqgsltadniQAVYHDgf---vyTLAYFRSR 750  Pseudom...
BAC97662            455 KDEMVALGAGIAStr-seYVNTSVNQTRl----NGPVTVDgavyekg-----sralvnaSWVHHDg------IGYVFPAY 518  Vibrio ...
ZP_00797056         764 ENRLVLLGSNISNntekyATETTLFQHGit-dkASDLWVNgeritalp---yqraleegDWLIDGh-----gNGYLLTKG 834  Yersini...
REF_mbpwusl:BT3350  708 DNRMICLGTGITNsnadyPTETTLFQTKf----NGKEQKTgk---------------dnYWLHDGy------DNYYHVVD 762  Bactero...
CAG22026            755 DNTVVALGSGIKNddsqhETETTLFQYSipnlePVEVNGNpirtlgt------dmtlttDSTFLDpa----gNRYFVKNI 824  Photoba...
AAO78430            746 DGMIVCLGSDIENtntdyPTETTIFQLAvtdkaAHDYWKNnag-------------egkVWMDHLg------TGYYVPVP 806  Bactero...

Feature 1                                                                                               
1F1S_A              677 STIDIErkEQTGTWnsinrtskNTSIVSn--pFITISQKhd--nkGDSYGYMMVPNIdRTSFDKLan-----skEVELLE 747  Strepto...
1N7R_A              581 SSISMSkaLQKGAWkdin-egqSDKEVEn--eFLTISQAhk--qnGDSYGYMLIPNVdRATFNQMik-----elESSLIE 650  Strepto...
1HN0_A              833 EKVNVSrqHQVSAEn------kNRQPTEg--nFSSAWIDhstrpkDASYEYMVFLDAtPEKMGEMaqkfrenngLYQVLR 904  Proteus...
AAX07421            508 ANTFVLs-SGIGYVpf----dsNRYTVStendLFKMYINhgvnpsKINYAYLLMPNVtDSNIVSEaak---aknNLKIES 579  Flavoba...
AAZ36518            751 PTVTIEvkSRSGAWsdin-ingSPYTVTl--pVFSLCIHhqk-geNGSYCYSVSPSEdLLDRALLp--------TATVFE 818  Pseudom...
BAC97662            519 WYGHMNnqTQSGNWydin-rgqANEVVSd--eVFMLRIGhswqptNASYQYIIVPNRtASQVEAYaq-----qsPIAVLS 590  Vibrio ...
ZP_00797056         835 AKGEVRrqHQVSANd------kSRDPTEg--nFSLAWLDhgakpqNAQYEYLMVLEAtPEGMQQLatdyragkkVYEVLR 906  Yersini...
REF_mbpwusl:BT3350  763 GTLRSQiaEQESRHe------kTREKTTg--tFSSAWIEhgnapkNATYEYMVLIQPsASDLDELkk-----tpAYEVWQ 829  Bactero...
CAG22026            825 ADEQVRfsYQAQNSnd----edDGQPTTg--qFASAVIEhgkapvDGHYEYAISIEAqDTINPIYtvlh--qdnKVHAVR 896  Photoba...
AAO78430            807 ARFEKNf-PQYSRMq------dTGKETKg--dWVSLIIDhgkapkAGSYEYAILPGTdRKTMTAFak-----kpAYSVLQ 872  Bactero...

Feature 1                                                                                         
1F1S_A              748 NSS--KQQVIYDKn--sQTWAVIKHDNQeslin-----nqfkmNKAGLYLVQKVg---------nDYQNVYYQP 803  Streptococcus...
1N7R_A              651 NNE--TLQSVYDAk--qGVWGIVKYDDSvstis-----nqfqvLKRGVYTIRKEg---------dEYKIAYYNP 706  Streptococcus...
1HN0_A              905 KDK--DVHIILDKl--sNVTGYAFYQPAsiedk-----wikkvNKPAIVMTHRQk---------dTLIVSAVTP 960  Proteus vulgaris
AAX07421            580 QNE--SCHAVYDSa--tSQYYIAFFKPYtlnlsg---glvvkvDNPCMIIINNN-----------AGKFYVSNP 635  Flavobacteriu...
AAZ36518            819 AGMa-DEHIVYDG----EAVMVSCFDAEltrrwaqeaghgfypEQPCVYIAEQQd---------aQVKLTCADP 878  Pseudomonas s...
BAC97662            591 NSN--TLQAVHHQg--lNVTGVIFHQPGsi-----------nlHDGSTLSVSQAsv--------vLIDQSAADP 641  Vibrio vulnif...
ZP_00797056         907 QDA--SAHIVRDNv--tQTTGYAAFSSVtpdeg-----vvkniAQPAIVMTQMQsh--------gQLKISGVTP 963  Yersinia pest...
REF_mbpwusl:BT3350  830 RDQ--TAHIVYDRk--tGITGYAVFEDYrsadd-----klvvaSIPAETMVMYAaeg------kkAIRLSVCDP 888  Bacteroides t...
CAG22026            897 SEQgvEAYAFFEPatlsNTNWVIASNTA---------------SQIMLKTNESSdqlslsvvnpdLALYNGQDP 955  Photobacteriu...
AAO78430            873 QDR--NAHILESPs--dRITSYVLFETPqsllpg---gllqrtDTSCLVMVRKEsa--------dKVLLTVAQP 931  Bacteroides t...

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