1HP0


Conserved Protein Domain Family
nuc_hydro_TvIAG
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cd02647: nuc_hydro_TvIAG 
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nuc_hydro_ TvIAG: Nucleoside hydrolases similar to the Inosine-adenosine-guanosine-preferring nucleoside hydrolase from Trypanosoma vivax. Nucleoside hydrolases cleave the N-glycosidic bond in nucleosides generating ribose and the respective base. Nucleoside hydrolases vary in their substrate specificity. This group contains eukaryotic and bacterial proteins similar to the purine specific inosine-adenosine-guanosine-preferring nucleoside hydrolase (IAG-NH) from T. vivax. T. vivax IAG-NH is of the order of a thousand to ten thousand fold more specific towards the naturally occurring purine nucleosides, than towards the pyrimidine nucleosides.
Links
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Statistics
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PSSM-Id: 239113
Aligned: 15 rows
Threshold Bit Score: 256.576
Created: 29-Apr-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitedimerization
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:DXDXXXDD motif is a hallmark of nucleoside hydrolase activity
  • Structure:1HP0_A, Trypanosoma vivax, inosine-adenosine-guanosine- preferring nucleoside hydrolase, bound with 3-deaza-adenosine contacts at 3.5A. Note the Ca2+ cofactor bound at the bottom of the active site.
  • Comment:Trypanosoma vivax, inosine-adenosine-guanosine- preferring nucleoside hydrolase (IAG-NH) is a homodimer
  • Comment:The T. vivax dimer contains two active sites. Kinetic studies show no co-operativty between the two sites supporting that they act independently.
  • Comment:1HP0, PDB D10 is the proposed general base, abstracting a proton from a nucelophilic water molecule
  • Comment:In inosine-adenosine-guanosine- preferring nucleoside hydrolase from Trypanosoma vivax (1HP0_A), the purine base is stacked nearly parallel between two tryptophans: (PDB W83 and PDB W260). This stacking sandwich is believed to promote the protonation of the purine base at N-7. It is not present in the inosine-uridine-preferring nucleoside hydrolases which use histidine (at a position equivalent to PDB H241 in the nucleoside hydrolase of Crithidia fasciculate, 2MAS_A) as the general acid in the catalysis.
  • Comment:The majority of this group of IAG-nucleoside hydrolases possess a tryptophan residue , at a position equivalent to 1HP0_A, PDB 260; the inosine-uridine-preferring nucleoside hydrolase of Crithidia fasciculata has a catalytic histidine (H241)at this position.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              #   ##                                     #                             
1HP0_A       15 KNVVLDHDGNLDDFVAMVLLasnt-ekvRLIGALCt------------DADCfv---eNGFNVTGKIMCLmhnnm----- 73  Trypanosoma vivax
AAM91461     32 HRILVDTDVDTDDLFAILYLlklnksefDLVGITLsa---------naWTNAg-----HAVNQVYDLLHMmdr------- 90  thale cress
AAM91461    509 KPVVFDMDMSAGDFLSLFYLlkvpvdkiDLKAIIVsptgwanaatidvVYDLlhmmgrDDIPVGLGDMLAlnqsdpifpp 588 thale cress
NP_925425     3 KPVIHDQDGHADDLVATLLLaly--pavDLRAVVVn------------SGDCip---qVSAAVLAALLDRlgk------- 58  Gloeobacter viol...
XP_475208   510 KPVIFDMDMSPGDFISLIYLlkvpteliDLKGILVsgngwanvasidiVYDIlhmmgrDDIPVGRGSTSAlgteslg--- 586 Japanese rice
BAB74984      4 QLVLMDHDGGVDDYLATMLLltm--dnvQLMGVVVt------------PADCyv---qPAVSATRKIIDLmgf------- 59  Nostoc sp. PCC 7120
XP_475208    75 VNAWTDAGHAVNHLYDMLYMmg-----rDDIPVGVg-----------gDGGIsgh-gtIHPNVGGYLPLIdqg------- 130 Japanese rice
BAB77813     37 TPLIIDDDGSQDGMTALAYMlan--pkfDIQAITIaqg-------iarPQSFa-----NNLERMLGRLDIsg-------- 94  Nostoc sp. PCC 7120
ZP_00319476   2 KNVYFNHDGGVDDLVSLLLLlqm--dniKITGVGVm------------GADSyl---ePALSATRKIIDRfgng------ 58  Oenococcus oeni ...
AAT38027    210 KPVIFDMDMSPGDFVSLIYLlktpieviDLKAVLVngngwaniasidiVYDIlhmmgrDDIPVGLGNTTAlgiptlg--- 286 Japanese rice

Feature 1                                            #                                          
1HP0_A       74 -----nlplfPIGKSAATAV-----------NPFPKEWRCLA--------------------------KNMDDm----PI 107 Trypanosoma vivax
AAM91461     91 -------ddiPVGVGGEGGIsddgtihsdvgGYFPIIEQGMTttgecryrqaipkglgglldidsnygFRKQFl----PQ 159 thale cress
AAM91461    589 vggckyvkaiPRGCGGFLDSdtly----glaRDLPRSPRRYTa------------------------eNSVTHg---aPR 637 thale cress
NP_925425    59 -------gavPVAWQHHALP-----------HPFPTAWQRESl-------------------------NYLRAf----PE 91  Gloeobacter viol...
XP_475208   587 ---ckyvsaiPQGSGGLLDSdtly----glaRSLPRSPRRYTa------------------------eNSVKYg---aPR 632 Japanese rice
BAB74984     60 -------shiPVAESTVRGI-----------NPFPRLYRRDS--------------------------FIVDHl----PI 91  Nostoc sp. PCC 7120
XP_475208   131 --------mtTFGPCRYRQA-----------IPLEGGGRLDId------------------------tNSGIRkg-flPQ 166 Japanese rice
BAB77813     95 ---------iPIGIGRSTPLag--------nNTFPEPIRAGA--------------------------DTFWSpfvqlPN 131 Nostoc sp. PCC 7120
ZP_00319476  59 -------ielAPAASNSRAA-----------HPFPKDWRMDA--------------------------FSFDAf----PI 90  Oenococcus oeni ...
AAT38027    287 ---cnnsyaiPHGSGGFIDSdtly----glaRSLPRSPRRYAp-------------------------ESLDH-----PE 329 Japanese rice

Feature 1                                                      #                                
1HP0_A      108 LNipenvelwdkikaenekyEGQQLLADLVMNse------eKVTICVTGPLSNVAWCIDKygEKFTSKVeECVIMGGav- 180 Trypanosoma vivax
AAM91461    160 GNrrytp---------lqqpTAQKVIVDKISEg--------PTTVILLGSHTNFALFLMSn-PHLKHNIqHIYIMGGgvr 221 thale cress
AAM91461    638 DTdr--------------peLRQPLAIEVWQNltksgngvsKITVLTNGPLTNLAKIISSd-KKSSSLIkEVYIVGGhin 702 thale cress
NP_925425    92 AEryy------------paaAGTRLLTETILAag------ePVTLLVTGPCTNLAAALRAa-PEIARHLeRVVLMAGgl- 151 Gloeobacter viol...
XP_475208   633 DTdhpelr------qplafeVWQFVKHQLDPNe--------KITILTNGPLTNLANIVLSd-RNASSVIkSVYVVGGhir 697 Japanese rice
BAB74984     92 LNqtelih------tplveeTGQDFMVRVLQEal------ePVTLMVTGPLTTVAVALDQa-PDIEAKIdKIVWMGGal- 157 Nostoc sp. PCC 7120
XP_475208   167 GNrryip---------lhqsTAQQVLIETISAg--------PTTVMLIGSHTNFAIFLMTh-PHLKKNVeHIYIMGGgvr 228 Japanese rice
BAB77813    132 TAplv------------itrPAAELIVEKVKRsl------tPVAILATGPLTNIAEALRLd-PTIINNIaVIEIMGGav- 191 Nostoc sp. PCC 7120
ZP_00319476  91 LNesgkik------tpvavnPAHLDLIEKLKSes------gKTTLIMTSPLSDLARAIKSd-PTIVDRIeELYWMGGtm- 156 Oenococcus oeni ...
AAT38027    330 DRq----------------pLALEVWQSVRKQldp----geKITVLTNGPLTNMANISLSd-RDASSVIeRVYVVGGlik 388 Japanese rice

Feature 1                                 #               # #                                   
1HP0_A      181 ----------------------dVRGNVFLP-----STDGTAEWNIYWDPASAKTVFgc--pgLRRIMFSLDSTNTVPVR 231 Trypanosoma vivax
AAM91461    222 sqnptgccpanstvaecqprqcgNRGNLFTDy----TSNPYSEFNIFADPFAAYQVFhs---gVPVTLVPLDATNTIPIN 294 thale cress
AAM91461    703 r-------------------eksDKGNIFTI-----PSNAYAEFNMFLDPLAAKTVLes---aLNITLVPLATQHKLSSF 755 thale cress
NP_925425   152 ----------------------eAGGNVAGEq----DHDGSAEWNLYCDPPAAAYCLqa---gLALDFVSLDVTNFVPMT 202 Gloeobacter viol...
XP_475208   698 d-------------------dsnTKGNVFSV-----PSNTYAEFNIFLDPLAAKTVLds---tLDITLIPLRAQRKAASF 750 Japanese rice
BAB74984    158 ----------------------nVPGNVEKSle--aGQDGSAEWNVYWDAVSAARVWqr---eIKIIMCPLDLTNDVPVT 210 Nostoc sp. PCC 7120
XP_475208   229 senptgccpk-nsttsctpqqcgDHGNLFTSy----RTNPNAEFNMFADPFAAYQVFhs---gIAITLVPLDATNTIPVN 300 Japanese rice
BAB77813    192 ----------------------fVPGNLPVLpyppfSTNTTAEFNIWADPLAAQEVFaaggqgLKIQLTPLDATNQIAFS 249 Nostoc sp. PCC 7120
ZP_00319476 157 ----------------------dNRGNVAEP-----EQDGTIEWNAYWDPEAVKIVWdt---kIPIHMVGLESTRQVPLT 206 Oenococcus oeni ...
AAT38027    389 d-------------------ggdENGNLFTV-----PSNKHAEFNIFLDPLAAKTVLes---dLKIALIPLTAQRKAASF 441 Japanese rice

Feature 1                                                               #                       
1HP0_A      232 Sp-----------YVQRFGeqtnfllsiLVGTMWAmcthcell----rdgdgyyAWDALTAAYVvd-------------- 282 Trypanosoma vivax
AAM91461    295 Qkffe----tfenNYQRTY---------EAQYVFLslkiardtwfddefyksyfMWDSFTAGVAvs-------------- 347 thale cress
AAM91461    756 QtmldrlysstktPEARFVkrll-vrlqALHQKHRry----------------tHIDMFLGEVLgavllggddaslkpkm 818 thale cress
NP_925425   203 Arfv-------etLRSRGGac------rLAAELLAgveq-----------qnyfFWDTLAALLTc--------------- 243 Gloeobacter viol...
XP_475208   751 Halleal-khaetPESRFVhhll-tllhDLQQKHQly----------------hHMDMFLGELLgavs------------ 800 Japanese rice
BAB74984    211 Selv------ykmGRQRHYpv-----sdLAGQCYAlvip-----------qdyyFWDVLATAYLghpe------------ 256 Nostoc sp. PCC 7120
XP_475208   301 Ee-----------FFYAFQqqq---styEAQYCFDslkmardtwfndefytsyfMWDSFTSGVAlss------------- 353 Japanese rice
BAB77813    250 Ra-----------DQQAWLatat-peskLAAEFLDfaltviq-----snndpnpAWDLVAAINLs--------------- 297 Nostoc sp. PCC 7120
ZP_00319476 207 Rs-----------VRQHWAlerqypiidLIGQGYAlvpplehf----etnstyfMWDVLTTLYSqf-------------- 257 Oenococcus oeni ...
AAT38027    442 Ravla-----aleDIQHTHes------kFVHELLSllqelqi------kqklyhHLDIFLGEILgavymve--------- 495 Japanese rice

Feature 1                                                                              
1HP0_A      283 -----qkVANVDPv------pIDVVvdkq-pneGATVrtdaenypltfVARNPea--------eFFLDMLL 333 Trypanosoma vivax
AAM91461    348 ------iMRNSANknnk-ngeNDFAem-----eYMNIt---------vVTSNKpygrs-dgsnpFFDNRRT 396 thale cress
AAM91461    819 raehikvIAEGDEsrd---gkILIDkl-----rGKQIkil------erVDLISis--------eSFASRLD 867 thale cress
NP_925425   244 ------ePDFLPRgrr--tltVRTDpp----aaGRLEk---------tASGVPa---------rVFEPDAD 284 Gloeobacter violaceus PCC...
XP_475208   801 ----lveGSNIKQslq--rkpISIVansttsidGQTVvdnqs-anlvkVLLDFnsee---yykrVANSLGD 861 Japanese rice
BAB74984    257 ----fyqLREWETe-------IITSgl----sqGRTKvvtgg-rkilaMDKVDk---------dAFYAYIL 302 Nostoc sp. PCC 7120
XP_475208   354 -----mrNDNNCQs------eNDFAel-----kYMNIt---------vITSNKpygih-dgsnpLFDDHVI 398 Japanese rice
BAB77813    298 ------ePDFSVEtsl--yleVDTTsdpg-ntqGQTRaipnl-ppnvlVSLNPsfnnlpfqpgqVFSYLQI 358 Nostoc sp. PCC 7120
ZP_00319476 258 -----ldVAETKRvl----sdVSIAge----saGRTFeseng-rpvtlVTKVNhd--------rFFALIDE 306 Oenococcus oeni PSU-1
AAT38027    496 -gsglkpSVELKPvsviantnKSTD--------GQIViskns-aklvrVLSDFdgei----yskQLANSLA 552 Japanese rice

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