1XPG,1XPG


Conserved Protein Domain Family
CIMS_like
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cd03310: CIMS_like 
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CIMS - Cobalamine-independent methonine synthase, or MetE. Many members have been characterized as 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferases, EC:2.1.1.14, mostly from bacteria and plants. This enzyme catalyses the last step in the production of methionine by transferring a methyl group from 5-methyltetrahydrofolate to L-homocysteine without using an intermediate methyl carrier. The active enzyme has a dual (beta-alpha)8-barrel structure, and this model covers both the N-and C-terminal barrel, and some single-barrel sequences, mostly from Archaea. It is assumed that the homologous N-terminal barrel has evolved from the C-terminus via gene duplication and has subsequently lost binding sites, and it seems as if the two barrels forming the active enzyme may sometimes reside on different polypeptides. The C-terminal domain incorporates the Zinc ion, which binds and activates homocysteine. Side chains from both barrels contribute to the binding of the folate substrate.
Links
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Statistics
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PSSM-Id: 239426
Aligned: 22 rows
Threshold Bit Score: 165.677
Created: 19-Dec-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
1XPG_B      437 PTTTIGsfpqtpevrkxrsky-------rkgeiskeeyeafiKEQIKKAIELqeeiGLDVLVHgefertdXVEFFAEkln 509 Thermotoga maritima
BAD59115      5 VATGVGswpgtd-------------------------preaaATVLGELPDLa--hLVELPERgv---gaDMIGRVSall 54  Nocardia farcini...
YP_055824     2 IATGIGslpgtd-------------------------mrgslAAMAETFDQLi--pLPELPARgv---gaHMIGRATavl 51  Propionibacteriu...
YP_181260     9 LPTMIGsmphtdpkaavdiit-------hylqdipvwpqlprRSCLEGMSAQfsqgLPGVKINqd----kVWIEPNPsfe 77  Dehalococcoides ...
ZP_00557891  12 LATGVGslpyaqaetalsqiw-------kalpqaphwpqlpqLGAESSFVGQylkvLIETGCIggfeqprFQVDAPDwte 84  Desulfitobacteri...
ABB16115      5 LKTGIGsmplvdatknleiik-------ealpqiphwpqmpqRGVAEHFVFQflrpLTEVGLLqve--gnKGIFKKDetf 75  Carboxydothermus...
NP_148345     7 QGQVLGgyprsryvrralrrl-------eqgeisavelmgvlNQASSAVIGAqvsqGFPYVVDgml-dwhDIFRPFAeaw 78  Aeropyrum pernix K1
ZP_00601443   7 KAYAPGiyprseelvqatrdl-------drgrttreaverqrQRDLASLLRAqeeaGLDYLSDgll-vwqDIFRPFCeaa 78  Rubrobacter xyla...
CAI49927      5 LATTPGlyplpdwakdelsdlkghqkddlisgdesdaivdayERARADVLEWqtdaGIDRVVEgql-rwdDMLAHPLcvh 83  Natronomonas pha...
YP_255487     4 YTALVGsypkpvkiakvisrl-------ksgkiekskayeelMKFEKRILEEvkeyELDYTTDgmv-gwdDIADLTTsfl 75  Sulfolobus acido...

1XPG_B      510 giattqn------gwvlsygsrCYRPpiiygtv------trpepxTLKEITYAQslt----ekpVKGXLTGpVTIXSWSY 573 Thermotoga maritima
BAD59115     55 vdlrfd--------tstrgyrlTPRPgavsrrar---dllradldALEEAWETSgsag--tgrvLKLQSAGpLTLAAQVE 121 Nocardia farcini...
YP_055824    52 vdlpvd--------hgpggwrlADSTdhaarsar---swlrsdldDLEEVLADKea-------sVKLSFAGpLTLATGLH 113 Propionibacteriu...
YP_181260    78 sel-------------eslyqaYLDNdfnkfpi------gaeyaaGLYELASRNlsp-----llVKGHITGpLTYCMSIK 133 Dehalococcoides ...
ZP_00557891  85 rma--------------hfyeyYLHAeagdpnalaefgftpegglGFNEFCDVLdqqgtqeallLKGQLSGpVTLGMQIT 150 Desulfitobacteri...
ABB16115     76 pekltffyekyfealekrdyafFAMPresge----------glylLVEKFNEYFpra-----laVKGQMAGpLSILLTLT 140 Carboxydothermus...
NP_148345    79 rnvtptg------llryfdnnfFYRIplftgep------eptdpvLAPRVLSYSqvae---pakLKVVVPGpVTFTLMSR 143 Aeropyrum pernix K1
ZP_00601443  79 rglepgp------ltrflntntFYRApaatgeppt--legplgppYFEAERLPR----------GRWVATLpSPHAMAAF 140 Rubrobacter xyla...
CAI49927     84 dsvetrg------ivryydnnnFYREpvvqda-------ltpdgdVADEIAAADg--------vNQAVLPGpYSLSELAT 142 Natronomonas pha...
YP_255487    76 egvekga------lerffdnnfYYRKivinqklgy--ksqndyvqYFKQVKEISkdl----kskIKIVILGpLTFLQLAE 143 Sulfolobus acido...

1XPG_B      574 Yred-------ipEREIAYQIALAINEEVKDLeea---gikIVQIDEPAFrekapi--------------kkskwpEYFE 629 Thermotoga maritima
BAD59115    122 LpnghraltdpgaVRDLADSLAEGLAGHAEEVrarl-gaevVLQLDEPSLgavlagtlrgvsaldtvralpepeaqAVLD 200 Nocardia farcini...
YP_055824   114 LragesiladgsaLADVTTSLAEGVSQLIAELrrrmprvtwTLQIDEPSApavlsgavptqs---glyrypamagsTAVQ 190 Propionibacteriu...
YP_181260   134 DpsgkdilydevlSDAATKLLKLKATWQEHFLsnic--rntIIFVDEPAMsaygsa--------------ylplsrEQVT 197 Dehalococcoides ...
ZP_00557891 151 DpnrkaayyddfqRDILVKSLRCHARWQTKRLaqyg--lpvLMSIDDPGLyaygasthltl-----drtvlieninEVAE 223 Desulfitobacteri...
ABB16115    141 DenkipafydpeiREAVVKTLVMSARWQVNFLkntg--rkvYLFVDDPAIanygsfthltl-----kreevvgvlkEIID 213 Carboxydothermus...
NP_148345   144 Nesg-------isDEELAEAIANLLADEVEKAvka---gagFVQVDEPILsepk-------------------atrDHAL 194 Aeropyrum pernix K1
ZP_00601443 141 Aadg-------ldAGELARRVIRPQIEWLAENgc------aLVVLQEAALfggn-------------------idlQPLG 188 Rubrobacter xyla...
CAI49927    143 Dehy-------gdDAAFLDAVADFLAAEAGALpd-----veTLFLLEPSLvtdap----------------ddgadERAS 194 Natronomonas pha...
YP_255487   144 Nnyy-------ddVGNIIDDYAKSTNSLLRDLidy----ydVVEVHEPSLfqkgi-----------------kgalLQSS 195 Sulfolobus acido...

1XPG_B      630 WAINafnlaanarpetQIHAHXCysd---fneIIEYIHQl-eFDVISIEASrsk------geIISAFEnfkg--wikQIG 697 Thermotoga maritima
BAD59115    201 RVIDaq--------saPVVLHSCad------rPPLALLRrtaAAALAVDVStlgt---adldHLGEALdsg---trlVLG 260 Nocardia farcini...
YP_055824   191 LWQTvvsg----cgeaEVGMHCCga------pIPWVLARkagFSTMWCEVAsll-----nldRVAGWVesg---arlGMG 252 Propionibacteriu...
YP_181260   198 GMFDevfs----gisgLKGVHCCgn------tDWSILMDt-rVDIINFDTYayanslsiytdELKAFIkrg---gavAWG 263 Dehalococcoides ...
ZP_00557891 224 GILAeg---------gIPGAHVCag------mDWTLLFDs-kIRVVNFDAYeymtsmavlaePLERFLqkg---gilSWG 284 Desulfitobacteri...
ABB16115    214 GILEeg---------gIPGVHSCag------iDWSIVTEa-gAQVVSVDAFnyldslfpykeEIKSLLntg---gilALG 274 Carboxydothermus...
NP_148345   195 LAAElagvi--asrsgSAETMLSvyfyppkpeVYEALLEa-rVDYLMVDLVa---------aGSKGLEvitgkgageGLA 262 Aeropyrum pernix K1
ZP_00601443 189 AALEel--------agPLPLALQlpfg-dageALGELVEl-nVEAIGVDFYs---------tDVEALPrpf----pkALL 245 Rubrobacter xyla...
CAI49927    195 EAIDavad----avdaEVVVHTYwgsi--pekPYAHLMDa-dIDAIGFDFVt---------dHEGSVYniqeygtkdSIA 258 Natronomonas pha...
YP_255487   196 QQVYeklf-----ngiNKEKHVLtyfd-irfdRIQYLLNl-pADYFGFDVTe---------qNKTKLGrlygymkdmNVY 259 Sulfolobus acido...

1XPG_B      698 VGVWDi-----HSPAv-------pSINEXREIVERVlrvl--pkelIWINPDCGLKTRNwde---vipSLRNXVALAKE 759 Thermotoga maritima
BAD59115    261 LVPTTepa-geFGWRea-----aePGVRLIDRLGFPrtll---athILVAPACGLAGAPlsw---arrALTLAAEVTRA 327 Nocardia farcinic...
YP_055824   253 VVDTMqvd-kvPLVDrl-----idRVLWLVRRLEIDpav----lksGILSPACGLAGWSrhd---aaeVCRTVKRAGDL 318 Propionibacterium...
YP_181260   264 IVPTDekalkeETAAsl-----kdRLEAAMSHFDSRglpfaelarhSLITPACGLGLKSpeaaerapqLLAELSALLRR 337 Dehalococcoides e...
ZP_00557891 285 IVPTSaka-wgESAAsl-----reRLETNIKELTKRgvsedrlrsqSMLTPSCGTGTLDiel---aerIYALLQELSTS 354 Desulfitobacteriu...
ABB16115    275 IIPTGpei-enYSLEdl-----ykDYERKVNLLREMgvvs--dnsnIMLTPACGTGLLLpeh---aekIYGLLRDFGKE 342 Carboxydothermus ...
NP_148345   263 LGVIDa-----RSIYses---lekVEAPVRRIVESVkp------ekIILTTSTWLDLIPyry---alrKTALLGRYTYA 324 Aeropyrum pernix K1
ZP_00601443 246 AGVIDa-----RNSLled----prEVAKFGRALLAEle------geLHLVPNGDLQFVPegi---arqKLARLGEAARI 306 Rubrobacter xylan...
CAI49927    259 LGVVDg-----QNTLvetpeeiaeRVEWVEEQVPVSef------eqLYVTSNTELFYLPtnk---aeaKLNALATAAAE 323 Natronomonas phar...
YP_255487   260 LGVIDs-----RNTKmdk----isTIRRIIEKVHEKga------ksLIIGNSSFNDFIPeiv---vrrKLKLLQKTKRL 320 Sulfolobus acidoc...
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