1J8F,1ICI,1S5P,1Q14,1MA3


Conserved Protein Domain Family
SIR2-fam

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cd01407: SIR2-fam 
Click on image for an interactive view with Cn3D
SIR2 family of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer.
Statistics
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PSSM-Id: 238698
Aligned: 100 rows
Threshold Bit Score: 195.48
Created: 10-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site [chemical binding site]
Evidence:
  • Structure:1MA3; Archaeoglobus fulgidus Sir2 homolog, Sir2_Af2, binds peptide substrate p53 with acetylated Lys382.
  • Comment:The acetylated Lys side chain has an extended conformation. H118, V163, F165, L169, and V196 side chains form van der Waals interactions with the aliphatic atoms of the Lys side chain. The acetyl methyl group is in van der Waals contact with I102 and H118.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                      
1J8F_C      44 RRVICLVGAGISTSAGIPDFRSPstGLYdnlekyhlpype-------aifeisyfkkhPEPFFALakely--pgqfkPTI 114  human
1MA3_A      16 KHAVVFTGAGISAESGIPTFRGEd-GLWrkydpeeva-------------sisgfkrnPRAFWEFsmemkd-klfaePNP 80   Archaeoglobus fu...
CAG04727   253 KRILVLTGAGVSVSCGIPDFRSRd-GIYarlavdfpdlpdp-----qamfdidyfrrdPRPFFKFakeiy--pgqfqPSL 324  Tetraodon nigrov...
NP_501912  146 KHILVLTGAGVSVSCGIPDFRSKd-GIYarlrsefpdlpdp-----tamfdiryfrenPAPFYNFareif--pgqfvPSV 217  nematode
EAA40294    22 ESIVILAGAGISVAAGIPDFRSKgtGLYsqlerynlptpt-------smfdlsyyclrPRPFSSLsvsifp-sykykPTM 93   Giardia lamblia ...
EAA67072  1763 TRIVALVGAGLSTSSGLADFRTPdtGLYakleplqlpype-------alfhisyfkhtPEPFYAIargrh--pwntkPGV 1833 Aspergillus nidu...
P53687      53 RRIACLTGAGISCNAGIPDFRSSd-GLYdlvkkdcsqywsiksgremfdislfrddfkISIFAKFmerlysnvqlakPTK 131  baker's yeast
EAA34475   122 KKIVVIAGAGISVSAGIPDFRSSt-GLFatlrgqhklkasgk---hlfdasvykhddsTESFHTMvrelaqltsqakPTP 197  Neurospora crassa
Q9UR39      58 KRIVVVTGAGISCDAGIPDFRSSe-GLFsslraeyklncsgk---elfdgsvyrdlksVNIFHAMirklhmlsnnarPTD 133  fission yeast
NP_597557   86 RRVVVITGAGISVSSGIPDFRSKs-GLFndikkdlgvsgnd-----lftyslsmskelRKGYLRYisklknmvdkaqPSA 159  Encephalitozoon ...
Feature 1                           #                                 #                        
1J8F_C     115 CHYFMRLLKdkglllrCYTQNIDTLERIAGLeq----------------edLVEAHGTFYT-SHCvs---asCrheypls 174  human
1MA3_A      81 AHYAIAELErmgivkaVITQNIDMLHQRAGSr------------------rVLELHGSMDK-LDCl-----dChetydws 136  Archaeoglobus fu...
CAG04727   325 CHKFISMLDkqgkllrNYTQNIDTLEQVAGVq------------------rIIQCHGSFAT-ASCl-----iCkhkvdce 380  Tetraodon nigrov...
NP_501912  218 SHRFIKELEtsgrllrNYTQNIDTLEHQTGIk------------------rVVECHGSFSK-CTCt-----rCgqkydgn 273  nematode
EAA40294    94 AHHFFKILEdrglvrfIYTQNIDELEIFAGVsp----------------rrILQCHGSYCKgLYClgnvsgvCtyqvedq 157  Giardia lamblia ...
EAA67072  1834 GHAFLALLEkkgvlgfVFTQNIDGLELDAGVsr----------------erVMNLHGDWSD-QHCi-----kCrssypad 1891 Aspergillus nidu...
P53687     132 THKFIAHLKdrnkllrCYTQNIDGLEESIGLtlsnrklpltsfsshwknldVVQLHGDLKT-LSCt-----kCfqtfpws 205  baker's yeast
EAA34475   198 FHHMLASMAeegrllrLYTQNIDTLDTQMPPlatnvpln-----akgpwpvTVQLHGGLEK-MVCt-----kCshlepfn 266  Neurospora crassa
Q9UR39     134 FHLFLSQLAqeskllrLYTQNIDFLETRLEGlqtciplp-----qsapwptTIPLHGTLEV-VSCt-----rCsflkkfn 202  fission yeast
NP_597557  160 THEFLSLYSdisrrfrIYTQNIDGLEEKAGLaatkd-----------rstrLVYLHGNMKS-LGCl-----yCgykiefg 222  Encephalitozoon ...
Feature 1                                                    # #####                           
1J8F_C     175 wmk-ekifsevtpkCe--dCqsl------------------vkPDIVFFGESLPar--fFSCMQSDFlk--vDLLLVMGT 229  human
1MA3_A     137 efv-edfnkgeiprCr--kCgsy-----------------yvkPRVVLFGEPLPqr--tLFEAIEEAkh--cDAFMVVGS 192  Archaeoglobus fu...
CAG04727   381 vir-edifnqvvphCs--rCpdipl--------------aimkPDIVFFGENLPem--fHRAMKQDKde--vDLLIVIGS 439  Tetraodon nigrov...
NP_501912  274 eir-eevlamrvahCk--rCegv------------------ikPNIVFFGEDLGre--fHQHVTEDKhk--vDLIVVIGS 328  nematode
EAA40294   158 nvfraaamdqsvshCp--rCgrv------------------lkPRIVFFGEQLPse--fQLAPEIIGdaektSMLLILGT 215  Giardia lamblia ...
EAA67072  1892 rmr-kailtgevpfCvqanCegi------------------vkPAIVMFGESLPeg--fDSREEEMLst--aDLLLVIGT 1948 Aspergillus nidu...
P53687     206 rywsrclrrgelplCp--dCealinkrlnegkrtlgsnvgilrPNIVLYGENHPsceiiTQGLNLDIikgnpDFLIIMGT 283  baker's yeast
EAA34475   267 ae---lfegseaplCa--kCkeqdevrttfa-gkrshgigrlrPRIVLYNEYNPdeeaiGNVSKADLkr-vpDAVIVVGT 339  Neurospora crassa
Q9UR39     203 pd---ifdrngvtvCp--dCktenevrriag--krsviegclrPRIVLYNEIHPdsesiGSVCSQDLks-rpDCLIVAGT 274  fission yeast
NP_597557  223 daerdaygrgeeivCa--gCvrrnekrgrd---vrkrpvgvfhTTIIHYNQSHPdssfiSKMAEHDNt---cDLFIVMGT 294  Encephalitozoon ...
Feature 1        ###                                                                           
1J8F_C     230 SLQVQPFASLISkap------lsTPRLLINKEKAgqsdpflgmimgl---------------gggmdfdskkayrDVAWL 288  human
1MA3_A     193 SLVVYPAAELPYiak-----kagAKMIIVNAEPTmad-----------------------------------pifDVKII 232  Archaeoglobus fu...
CAG04727   440 SLKVRPVALIPNsip------heVPQVLINREQLphl------------------------------------nfDVELL 477  Tetraodon nigrov...
NP_501912  329 SLKVRPVALIPHcvd------knVPQILINRESLphy------------------------------------naDIELL 366  nematode
EAA40294   216 SLTVAPFNFLAEmvp------neALRVLINRDPAgyvnqalrfyanahpektaadvfaneqlmqealdlvgrdgmDMFLP 289  Giardia lamblia ...
EAA67072  1949 SLKVAPCSEIPRrlp------shVPRVLVNRELVgnig---------------------------------tresDVCLL 1989 Aspergillus nidu...
P53687     284 SLKVDGVKQLVKklskk-ihdrgGLIILVNKTPIgessw--------------------------------hgiiDYQIH 330  baker's yeast
EAA34475   340 TLKIPGVRRLVKemcqltrsrrdGITAWINIDPEpqgae-------------------------------fkdcwDYVIR 388  Neurospora crassa
Q9UR39     275 SCKIPGVKRIIKemsnc-vhkqkGNVIWLNYDEPtkdf---------------------------------lnlcDLVVQ 320  fission yeast
NP_597557  295 SLKVFGVRRLVKyfcrl--nstrGRRILVNLDRPskef---------------------------------gelfDFFWN 339  Encephalitozoon ...
Feature 1        
1J8F_C     289 GE 290  human
1MA3_A     233 GK 234  Archaeoglobus fulgidus
CAG04727   478 GD 479  Tetraodon nigroviridis
NP_501912  367 GN 368  nematode
EAA40294   290 GD 291  Giardia lamblia ATCC 50803
EAA67072  1990 GD 1991 Aspergillus nidulans FGSC A4
P53687     331 SD 332  baker's yeast
EAA34475   389 GK 390  Neurospora crassa
Q9UR39     321 GD 322  fission yeast
NP_597557  340 GD 341  Encephalitozoon cuniculi

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