6PC3,3HI0,6PC0,6PC1,6PC2


Conserved Protein Domain Family
ASKHA_NBD_HpPPX-GppA-like
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cd24052: ASKHA_NBD_HpPPX-GppA-like 
nucleotide-binding domain (NBD) of Helicobacter pylori exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (HpPPX/GppA) and similar proteins
The PPX/GppA family proteins play essential roles in bacterial survival and metabolism. Guanosine pentaphosphate (pppGpp) phosphohydrolase (GppA; EC 3.6.1.40) plays a key role in (p)ppGpp homeostasis. It specifically catalyzes the conversion of pppGpp to ppGpp (guanosine tetraphosphate). Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX; EC 3.6.1.11), which mediates the metabolism of cellular inorganic polyphosphate. Especially, it is responsible for the maintenance of appropriate levels of cellular inorganic polyphosphate (PolyP). The family corresponds a group of proteins similar to Helicobacter pylori PPX/GppA (HpPPX/GppA). HpPPX/GppA is phylogenetically distant from the Escherichia coli homologs. Unlike E. coli that possesses two homologs, EcGppA and EcPPX, H. pylori encodes only one PPX/GppA homolog, HpPPX/GppA. As such, HpPPX/GppA may play important roles in the homeostasis of both (p)ppGpp and PolyP.
Links
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Statistics
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PSSM-Id: 466902
Aligned: 188 rows
Threshold Bit Score: 243.157
Created: 19-Dec-2022
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is located in a cleft between catalytic domains I and II of the N-terminal domain (NTD) of PPX/GppA.
  • Structure:6PC1; Helicobacter pylori PPX/GppA in complex with ppGpp, LMR and phosphate ion at active site, contacts at 4A
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1               ### #                    #                                               
6PC3_A        16 TTVIDIGSNSVRLAVFKKts--qFGFYLLFETKSKVRIsegc------yafnGILQEIPMQRAVKALSEFKEIALKYksK 87  Helicobacter py...
6PC1_A        16 TTVIDIGSNSVRLAVFKKts--qFGFYLLFETKSKVRIsegc------yafnGILQEIPMQRAVKALSEFKEIALKYksK 87  Helicobacter py...
ALI37695      14 LAIIDLGYNSIKISTYDIyk--nGHYKKQYQKQDYVQIgsel------irndNIISDKNVDRTIKALDNFKKDLKEKniD 85  Candidatus Nitr...
AHC52079       3 YAVIDTGYNSTRLCIYDLfp--nQTFRILGSQKLFLRLgegv-------kegSPISPDRVNEAEKVFKSFRAILNKMgiR 73  Sulfolobus acid...
WP_048099103   4 IAVIDLGYNSIRLSLFQRns--rNSFRTLGSMKNFTRLgegv-------degGDIKEDKVQNAEKIMAKFRDVLERRgiE 74  Candidatus Acid...
OUW99511      22 ASVIDLGSNSVKMVNYNVds--qNTYKPYHQESSRIKLaeg--------lvdEVIQQSHIEKTIESLKLFRSIIDFEqiD 91  Flavobacteriale...
RPJ29189       3 ISIIDIGSNTIKLVIYEVrk--dNSFIGVHQESTKVKLgeal-------aysDSLNEQSMLKSINVLLQYRDIAKLEaaN 73  Nitrosopumilale...
TRZ69810       3 ISVIDLGFNSAKLVIYNVkd--dDSFDVYQQEGIKVRLgegl-------dekGILGKKSMSRAIDTLQVFRDVIQLNpiK 73  Nitrosopumilace...
KAA1298027    36 FAVIDIGSNSVTLSIFRSlskskKTLNNIHSKKIYFGLqnyv-------nssKDEKFHAIIKLMDIVMGMKKISKEYn-A 107 SAR202 cluster ...
WP_013867251   9 ISVIDIGSNTVKLVIYKYk---nGKLIPKYTQSIYLRLfnyliksnsphgekLIISKKGISKTKEVLEHFKKEVDEYkpD 85  Methanothermoco...

Feature 1              ##                               #                       ####             
6PC3_A        88 KILCVATSAVRDApNRLEFVARVKKa-cGLQIKIIDGQKEALYGGIACANLlh-knSGITIDIGGGSTECALIEKgkIKD 165 Helicobacter py...
6PC1_A        88 KILCVATSAVRDApNRLEFVARVKKa-cGLQIKIIDGQKEALYGGIACANLlh-knSGITIDIGGGSTACALIEKgkIKD 165 Helicobacter py...
ALI37695      86 VVIPIATSAVRDAsNQKFVVDTLEDs-tGFLFNILSGPEEGFFSYLGAQSYmh-ipNGLFFDLGGGSLELMYVQDfrILK 163 Candidatus Nitr...
AHC52079      74 DVKLVGTSAFRYAsNGNQVAELLSEi-iGVKMRVLTGEEEGEMSAVGVLNTlp-vdSGAMFDLGGGSLEVVYFDSrqVKE 151 Sulfolobus acid...
WP_048099103  75 TVYPLGTSAFRLArNGEEVAKRLSKs-lGWDVEILPGEEEGRLAALGALNSlp-fsDGIIFELGGGSLEIAYVKNrsLGK 152 Candidatus Acid...
OUW99511      92 YVLAVATSAVRDAkNNVDFIDQIRKe-tGFNFKILSEHEEAIYSHAGAIRSln-lpSTVFFDIGGGSLEIVSSKNfeIQK 169 Flavobacteriale...
RPJ29189      74 KVLCVGTSAVREArNGQYFIDQVLKk-tGFGIRVLNGEEEAYYSYLGASMMtc-ipNGLFFDLGGGSLELVYTEDfnVKK 151 Nitrosopumilale...
TRZ69810      74 SVLPIATSAVREAnNRNEFLNNVSKe-tGFNFKVLSEREEALYSYVGAIKSlh-tpDALFFDIGGGSLEIVYAERfkIKK 151 Nitrosopumilace...
KAA1298027   108 RLKIIATSAMREV-SKELDIKNIFLeltRTEIKILSSKQEGYYSFSGAIEKikpkkGILLFDLGGGSLEIGIFDNkkIKD 186 SAR202 cluster ...
WP_013867251  86 KLIAFATYVIRVAdNKDEFLNEMKD---YFDIRILSEQEEAYYSAYGALLNin-leSGIICDMGGGSLEVCSVKNndIEH 161 Methanothermoco...

Feature 1                                                                       ##  #   #        
6PC3_A       166 LISLDVGTIRIKEMFldkd-----------ldvKLAKAFIQKEVSKlp------fKHKNAFGVGGTIRALSKVLMKRFdy 228 Helicobacter py...
6PC1_A       166 LISLDVGTIRIKEMFldkd-----------ldvKLAKAFIQKEVSKlp------fKHKNAFGVGGTIRALSKVLMKRFdy 228 Helicobacter py...
ALI37695     164 TICLDMGVLRLSEEFvnynngveesetwsninyERLEKSLVENIPSkfqlslgrsPDTKMVAIGGTIRAIYKFMSHMIyt 243 Candidatus Nitr...
AHC52079     152 VYHFDLGALKLARELkd---------------eSELRKRIRSELSVlk------pIKGVLVGSGGNIRAIAKMDARVSaf 210 Sulfolobus acid...
WP_048099103 153 IFHFPLGALRLTKLYkn---------------qEEMRKEIRGYLYSlp-----iwIPPTLIGSGGNVRSVGRFIMQMDnv 212 Candidatus Acid...
OUW99511     170 VISLPLGSLRLTQQFsndsef-------sektiSKIKEFVQKELPTreflgisdsDDVVLVGVGGVLRAIAKYNQEKIny 242 Flavobacteriale...
RPJ29189     152 AEALPLGALRLSRTFankdgs------fskkncDNMTQRILDTLPTkkny--gigIDTLLVGAGGTLRALGRYDQKHSgf 223 Nitrosopumilale...
TRZ69810     152 VISLPLGALRLTHQFdgrkgk------ftdknfKDLKRYVWNLLPNkkdl--glsKDVRLVGVGGALRALARYEQRISry 223 Nitrosopumilace...
KAA1298027   187 IESIILGSLQLKIKFfskdky-------tsleiENCRKYIKGKLEKfyk----ydSYNKIIGIGGTVRNLGKIKISNEkk 255 SAR202 cluster ...
WP_013867251 162 CNSYPLGTLYFKDFFpdgi----------lineEGARNKIRKFIKKps------qNFETMVGVGGSIRALSKITHKKKie 225 Methanothermoco...

Feature 1                                                #                          #   #        
6PC3_A       229 pidsl-hgyeidaHKNLAFIEKIVMLKEDQLRLLGvn-eERLDSIRSGALILSVVLEHLkTSLMITSGvGVREGVFLSDL 306 Helicobacter py...
6PC1_A       229 pidsl-hgyeidaHKNLAFIEKIVMLKEDQLRLLGvn-eERLDSIRSGALILSVVLEHLkTSLMITSGvGVREGVFLSDL 306 Helicobacter py...
ALI37695     244 apsfyhsravldkRMITLANTIFRGLSIEELSKLKsvdpERAKTITTGSFIVKILINKQnFNNLLVCPtGVREGVLEYYL 323 Candidatus Nitr...
AHC52079     211 plksi-hgyviktKQIGKYSKVLMNLDPEERENLPgiskERSYTVHTASVVIEELGNALdSPTIMISSfGLREGVLMERV 289 Sulfolobus acid...
WP_048099103 213 kfnhv-hgyviprDKISSLAKSIWTMNPEEIASFPgigkERALTVKASLLVMEELTTFFdSQNIMVSEfGMREGKIMRGE 291 Candidatus Acid...
OUW99511     243 plkkl-hnfslglEPLDNIATELLNQNTEKISKIEsiggSRSDTIKAGSIVISELMKKLsFPSLIVSAqGLREGTLSLSL 321 Flavobacteriale...
RPJ29189     224 pfeki-hnyrltlNTIDSLRKDFSSLKPSEISSRWsfdsTRVETVVAGIHVIHSIMKKFdFEEVIISGyGIREGILASFI 302 Nitrosopumilale...
TRZ69810     224 pldki-hnykinrNSLDSTINELSKMKSDDISKISvigsSRSETIIAGSCVIETLMDKYnFSDLYVSNhGLREGALSIFL 302 Nitrosopumilace...
KAA1298027   256 qsskn--nleitkLGLEKIIKMISKYDKHKLSNMKglnpNRSDTILPGLLVVSEIMTKSkAKKLQISSnGLREGYMLRSN 333 SAR202 cluster ...
WP_013867251 226 ------------kTVLDKTLNKIKNMTPNEISEKYmiseRRSETVVSAALAMSELMNIYgCKTLYISKyGIREGIILKLL 293 Methanothermoco...

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