1HV6,1QAZ


Conserved Protein Domain Family
AlgLyase

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cd00244: AlgLyase 
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Alginate Lyase A1-III; enzymatically depolymerizes alginate, a complex copolymer of beta-D-mannuronate and alpha-L-guluronate, by cleaving the beta-(1,4) glycosidic bond.
Statistics
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PSSM-Id: 238148
Aligned: 9 rows
Threshold Bit Score: 513.289
Created: 4-Sep-2001
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:residues important for proposed catalytic mechanism for polymannuronic acid degradation
  • Structure:1HV6; alginate lyase with bound trisaccharide
  • Comment:1QAZ_A apo form

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                #       #            
1HV6_A     19 DVKARRTFLQSGQLDDRLKAALp-kEYDCtteatPNPQQGEMVIPRRYLSGNHGPVNpDYEPVVTLYRDFEKISATLGNL 97  Sphingomonas sp.
1QAZ_A     19 DVKARRTFLQSGQLDDRLKAALp-kEYDCtteatPNPQQGEMVIPRRYLSGNHGPVNpDYEPVVTLYRDFEKISATLGNL 97  Sphingomonas sp.
JN0777     24 VSQAADLVPPPGYYAAVGERKGs--AGSCpa--vPPPYTGSLVFTSKYEGSDSARAT-LNVKAEKTFRSQIKDITDMERG 98  Pseudomonas aerugi...
O50660     20 VHAASALVPPKGYYAALEIRKGe--AQACqa--vPEPYTGELVFRSKYEGSDSARST-LNKKAEKAFRAKTKPITEIERG 94  Azotobacter chrooc...
3913014    21 VHAAEALVPPKGYYAPVDIRKGe--APACpv--vPEPFTGELVFRSKYEGSDAARST-LNEEAEKAFRTKTAPITQIERG 95  Azotobacter vinela...
3915602    24 VSQAADLVPPPGYYAAVGERKGs--AGSCpa--vPPPYTGSLVFTSKYEGSDSARAT-LNVKAEKTFRSQIKDITDMERG 98  Pseudomonas aerugi...
BAB03312   70 DVKARRTFLQSGQLDDRLKAALp-kEYDCtteatPNPQQGEMVIPRRYLSGNHGPVNpDYEPVVTLYRDFEKISATLGNL 148 Sphingomonas sp.
Q9L7P2     24 TGASAALVPPKGYDAPIEKMKTgdhNFSCea--iPKPYTDKLVFRSKYEGSDKARAT-LNAVSEEAFRDATKDITTLERG 100 Pseudomonas syring...
Q9ZNB7     24 TQAAAPLRPPQGYFAPVDKFKTgdkSDGCda--mPAPYTGPLQFRSKYEGSDKARAT-LNVQSEKAFRDTTKDITTLERG 100 Halomonas marina
Feature 1                                             #   #  #                                
1HV6_A     98 YVAt-------GKPVYATCLLNMLDKWAKADALLNYDPKSQSWYQVEWSAATAAFALSTMmaep----nvdTAQRERVVK 166 Sphingomonas sp.
1QAZ_A     98 YVAt-------GKPVYATCLLNMLDKWAKADALLNYDPKSQSWYQVEWSAATAAFALSTMmaep----nvdTAQRERVVK 166 Sphingomonas sp.
JN0777     99 ATKlvtqymrsGRDGDLACALNWMSAWARAGALQSDDFNHTGKSMRKWALGSLSGAYMRLkfsssrplaahAEQSREIED 178 Pseudomonas aerugi...
O50660     95 VSRmvmrymekGRLRRAGMRPGLLDAWAEDDALLSTEYNHTGKSMRKWALGSLAGAYLRLkfstsqplaayPEQAKRIEA 174 Azotobacter chrooc...
3913014    96 VSRmvmrymekGRAGDLECTLAWLDAWAEDGALLTTEYNHTGKSMRKWALGSLAGAYLRLkfsssqplaayPEQARRIES 175 Azotobacter vinela...
3915602    99 ATKlvtqymrsGRDGDLACALNWMSAWARAGALQSDDFNHTGKSMRKWALGSLSGAYMRLkfsssrplaahAEQSREIED 178 Pseudomonas aerugi...
BAB03312  149 YVAt-------GKPVYATCLLNMLDKWAKADALLNYDPKSQSWYQVEWSAATAAFALSTMmaep----nvdTAQRERVVK 217 Sphingomonas sp.
Q9L7P2    101 VSKvvmqymrdGRPEQLDCALNMMTTWAKADALESREFNHTGKSMRKWALGSMSSAYLRLkfseshplanrQQDAKIIET 180 Pseudomonas syring...
Q9ZNB7    101 TAKrvmqfmrdGRPEQLECTLNWLTAWAKADALMSKDFNHTGKSMRKWALGSMASSYIRLkfsdshplaqhQQEAQLIEA 180 Halomonas marina
Feature 1                           ##                                                    ##  
1HV6_A    167 WLNRVARHQTSFPGGDTSC-CNNHSYWRGQEATIIGVISKDDELFRWGLGRYVQAMGLINEDGSFVHEMTRHEQSLHYQN 245 Sphingomonas sp.
1QAZ_A    167 WLNRVARHQTSFPGGDTSC-CNNHSYWRGQEATIIGVISKDDELFRWGLGRYVQAMGLINEDGSFVHEMTRHEQSLHYQN 245 Sphingomonas sp.
JN0777    179 WFARLGTQVVRDWSGLPLKkINNHSYWAAWSVMSTAVVTNRRDLFDWAVSEFKVAANQVDEQGFLPNELKRRQRALAYHN 258 Pseudomonas aerugi...
O50660    175 WFAKVGDQVIKDWSDLPLKqINNHSYWAAWSVMAAGVATNRRPLFDWAVEQFHIAAKQVDPRGFLANELKRRQRALAYHN 254 Azotobacter chrooc...
3913014   176 WFAKVGDQVIKDWSDLPLKrINNHSYWAAWAVMAAGVATNRRPLFDWAVEQFHIAAGQVDSNGFLPNELKRRQRALAYHN 255 Azotobacter vinela...
3915602   179 WFARLGTQVVRDWSGLPLKkINNHSYWAAWSVMSTAVVTNRRDLFDWAVSEFKVAANQVDEQGFLPNELKRRQRALAYHN 258 Pseudomonas aerugi...
BAB03312  218 WLNRVARHQTSFPGGDTSC-CNNHSYWRGQEATIIGVISKDDELFRWGLGRYVQAMGLINEDGSFVHEMTRHEQSLHYQN 296 Sphingomonas sp.
Q9L7P2    181 WFSKLADQVVSDWSNLPLEkINNHSYWAAWSVMATAVATNRQDLFDWAVKEYKVAANQVDKDGFLPNEMKRRQRALSYHN 260 Pseudomonas syring...
Q9ZNB7    181 WFSKMADQVVSDWDNLPLEkTNNHSYWAAWSVMATAVATNRRDLFDWAVKEYKVGVNQVDADGFLPNELKRQQRALAYHN 260 Halomonas marina
Feature 1     #                                                         #       #             
1HV6_A    246 YAMLPLTMIAETASRQGIDLyaykENGRDIHSARKFVFAAVKNPDLIKKYAS-EPQDTRAFKPGrgDLNWIEYQRARFGF 324 Sphingomonas sp.
1QAZ_A    246 YAMLPLTMIAETASRQGIDLyaykENGRDIHSARKFVFAAVKNPDLIKKYAS-EPQDTRAFKPGrgDLNWIEYQRARFGF 324 Sphingomonas sp.
JN0777    259 YALPPLAMIAPFAQVNGVDLr--qENHGALQRLAERVMKGVDDEETFEEKTG-EDQDMTDLKVDn-KYAWLEPYCALYRC 334 Pseudomonas aerugi...
O50660    255 YSLPPLMMIAAFAQANGVDLr--gDNDGALGRLAGNVLAGVEDPEPFAERAG-EDQDMEDLETDa-KFSWLEPYCALYAC 330 Azotobacter chrooc...
3913014   256 YSLPPLMMVAAFALANGVDLr--gDNDGALGRLAGNVLAGVEKPEPFAERAGdEDQDMEDLETDa-KFSWLEPYCALYSC 332 Azotobacter vinela...
3915602   259 YALPPLAMIAAFAQVNGVDLr--qENHGALQRLAERVMKGVDDEETFEEKTG-EDQDMTDLKVDn-KYAWLEPYCALYRC 334 Pseudomonas aerugi...
BAB03312  297 YAMLPLTMIAETASRQGIDLyaykENGRDIHSARKFVFAAVKNPDLIKKYAS-EPQDTRAFKPGrgDLNWIEYQRARFGF 375 Sphingomonas sp.
Q9L7P2    261 YALPPLAMIASFAQANGVDLr--pENNGALKRLGDRVLAGVKDPSIFAEHNG-EKQDMTDLKKDp-KFAWLEPYCSLYTC 336 Pseudomonas syring...
Q9ZNB7    261 YALPPLAMIASFAQINGVDLr--qENNGALKRLGDRVLAGVKDPDEFEEKNG-KKQDMTDLKEDm-KFAWLEPFCTLYTC 336 Halomonas marina
Feature 1                       #         
1HV6_A    325 AD----ELGFMTVPIFDPRTGGSATLLA 348 Sphingomonas sp.
1QAZ_A    325 AD----ELGFMTVPIFDPRTGGSATLLA 348 Sphingomonas sp.
JN0777    335 EPnacsRPKKDREPFNSFRLGGEVTRVF 362 Pseudomonas aeruginosa
O50660    331 SPal-rERKAEMGPFKNFRLGGDVTRIF 357 Azotobacter chroococcum (strain mcd 1)
3913014   333 SPal-rERKAEMGPFKNFRLGGDVTRIF 359 Azotobacter vinelandii
3915602   335 EPkm-lEAKKDREPFNSFRLGGEVTRVF 361 Pseudomonas aeruginosa
BAB03312  376 AD----ELGFMTVPIFDPRTGGSGTLLA 399 Sphingomonas sp.
Q9L7P2    337 SPdv-lEEKHEKQPFKTFRLGGDLTKVY 363 Pseudomonas syringae pv. syringae
Q9ZNB7    337 APdv-iEKKRDMQPFKTFRLGGDLTKVY 363 Halomonas marina

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