PRY/SPRY domain in tripartite motif-containing domain 25 (TRIM25)
This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM25 proteins (composed of RING/B-box/coiled-coil core and also known as RBCC proteins). TRIM25 (also called Efp) ubiquitinates the N terminus of the viral RNA receptor retinoic acid-inducible gene-I (RIG-I) in response to viral infection, leading to activation of the RIG-I signaling pathway, thus resulting in type I interferon production to limit viral replication. It has been shown that the influenza A virus targets TRIM25 and disables its antiviral function.
Comment:Comparison with other B30.2/SPRY structures and analysis of the loop regions identified a putative binding pocket likely to be involved in binding target proteins
Structure:Mus musculus SPRY domain of TRIM25 ; key residues tested by mutagenesis for binding to RIG-I caspase recruitment domains (CARDs)
Jiyao W et al.(2023). "The conserved domain database in 2023.",