2AFF,1R21


Conserved Protein Domain Family
FHA_Ki67
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cd22673: FHA_Ki67 
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forkhead associated (FHA) domain found in proliferation marker protein Ki-67 and similar proteins
Ki-67, also called antigen identified by monoclonal antibody Ki-67, antigen KI-67, or antigen Ki67, acts as a biological surfactant to disperse mitotic chromosomes. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Ki-67 binds DNA with a preference for supercoiled DNA and AT-rich DNA. It may also play a role in chromatin organization. Ki-67 contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.
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Statistics
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PSSM-Id: 438725
Aligned: 57 rows
Threshold Bit Score: 80.3349
Created: 4-Sep-2020
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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phosphopeptideheterodimer
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphopeptide binding site [polypeptide binding site]
Evidence:
  • Structure:2AFF; Homo sapiens Ki67 in complex with phosphorylated hNIFK through its FHA domain, contacts at 4A
  • Comment:residues important for pThr234 binding in Ki67-FHA
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                      #           ##  #                                  
2AFF_A          5 RRLVTIKRSgv-dGPHFPLSLs--TCLFGRGIECDIRIQL-PVVSKQHCKIEIHe----------------QEAILHNFs 64   human
KFM79986        7 AQIFCIKLDgs-eSVPFIMKKd--RLLVGRGDSCEIRVHV-DGVASEHCAITMDq---------------eGRAYLEPLd 67   Stegodyphus m...
GBL98194        5 GRLYSLRKDgtkdSLYLNIKNk--RFLFGSSEYCTVRIYI-NGIFPEHCVLTIGg---------------qGKAYLEPLh 66   Araneus ventr...
XP_641637       2 ETLRSFHLVlp-sSQVVEIEQg-kDFLFGSSLDCNLRLQI-YGCSREHAIIKFNk---------------qGRPTISNLs 63   Dictyostelium...
XP_021708360    7 ARLCVLRKKgdgvIREVPIRTn--FVKIGSEVHCTIRLKS-ELTEQLHCKIFVRn----------------EKVMIANYs 67   yellow fever ...
CRK86951        8 GELLLLKSDgs-dGCTYATSEk--CITVGTSLKCQIKYHG-EDVKPEHFSIYVDd---------------yGQVKIANRs 68   Clunio marinus
XP_002074129    6 AQLCYVDKD----GVALSVAAkgrTFQIGSSMGCDILLTE-DYVDRLLCEINCDa---------------fGRVTIHNHs 65   Drosophila wi...
GAO47242        6 GTLLIPAHDdsnrISPFPVLKe--EIKIGRNISCDVRIHD-RDVSRTHCVIAFRdasapstpaksntaasgRRAWISNTg 82   Saitoella com...
OLL22375        8 GALHIINSDe--eNETFNLKRa--KTTFGKNIECDVKMWKsPYLSDRQCEINFDkr-------------gqLKATCSNIg 70   Neolecta irre...
XP_026477973    6 ANLVHLKKGv--pDKKLEIISd--DVTIGSDLRCTVRIHK-ADVERKHCTIKRNg----------------SKVILNNCs 64   cat flea

Feature 1          ##                         #       
2AFF_A         65 sTNPTQVNGSVIDe--pVRLKHGDVITIIDRSFRYE 98   human
KFM79986       68 nCGLTFLNNSVIAe--kILLEHNDMFKVVTRSFRFA 101  Stegodyphus mimosarum
GBL98194       67 pSALTLVNGIKIKh--kVCLEDNDVVTIFIKEFQFA 100  Araneus ventricosus
XP_641637      64 kFNVTCLNGSTVEngtsALLNDGDTIVMYGKKFTFK 99   Dictyostelium discoideum AX4
XP_021708360   68 sKNPITVDGKVINk--rGPLSDGSVIEVCGTRFRWQ 101  yellow fever mosquito
CRK86951       69 tENPVKVNDVKVEn--kRFLKSGDVIDLLGFKMRWK 102  Clunio marinus
XP_002074129   66 tKHTVQFNDEKLEg--kRPLLHGTRLTILDNVYTWK 99   Drosophila willistoni
GAO47242       83 -THGTTLNGAALTk--kTALEDGDVFEVLGNQFQWI 115  Saitoella complicata NRRL Y-17804
OLL22375       71 -SNDIQINGVALKqtqsRVLNDNDVIIIFDHEFRFT 105  Neolecta irregularis DAH-3
XP_026477973   65 qSTNILVNDKLVLs--dIELKDNDVLSVSNRKFLWS 98   cat flea

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