1H75,1R7H,1TTZ


Conserved Protein Domain Family
NrdH
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cd02976: NrdH 
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NrdH-redoxin (NrdH) family; NrdH is a small monomeric protein with a conserved redox active CXXC motif within a TRX fold, characterized by a glutaredoxin (GRX)-like sequence and TRX-like activity profile. In vitro, it displays protein disulfide reductase activity that is dependent on TRX reductase, not glutathione (GSH). It is part of the NrdHIEF operon, where NrdEF codes for class Ib ribonucleotide reductase (RNR-Ib), an efficient enzyme at low oxygen levels. Under these conditions when GSH is mostly conjugated to spermidine, NrdH can still function and act as a hydrogen donor for RNR-Ib. It has been suggested that the NrdHEF system may be the oldest RNR reducing system, capable of functioning in a microaerophilic environment, where GSH was not yet available. NrdH from Corynebacterium ammoniagenes can form domain-swapped dimers, although it is unknown if this happens in vivo. Domain-swapped dimerization, which results in the blocking of the TRX reductase binding site, could be a mechanism for regulating the oxidation state of the protein.
Links
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Statistics
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PSSM-Id: 239274
Aligned: 60 rows
Threshold Bit Score: 49.916
Created: 27-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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catalytic
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residues [active site]
Evidence:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                #  #                                                                   
1H75_A        2 RITIYTRNDCVQCHATKRAMEn-rGFDFEMINVDr--VPEAAEALraq--gfrQLPVVIAGd--LSWSGFRPd----mIN 70  Escherichia coli
AAF11632      1 MVKMYTTTWCPDCHAAKRALTq-kGIAYEEINIEq--DEQAAEYVmsvnggrrSVPTLVSGdvaHSLSGFRPq----kLD 73  Deinococcus radi...
CAC01317      4 TVTMYSTTWCGYCRRLKSQMDr-eGIAYTEVNIEq--DPESAAFVekanggnqTVPTVLFPd-gSTLTNPSLa----qVK 75  Streptomyces coe...
CAF19548      3 NVTIYATDWCPYCRSLLKGLD---GQEYDLIDVDq--DEEAGEWVksvndgnrIVPTVRYSd-gTHATNPLAaeviakIE 76  Corynebacterium ...
NP_962232     5 PLTVYTTSWCGYCHRLMTVLKs-nGIPYEAVDIEh--DPAAADFVssvnggnkTVPTVKFAd-gSTLTNPSAa----qVK 76  Mycobacterium av...
ZP_00151591  50 EVLLYATDWCGYCKMSREFFAa-nGIRYTEYDIEk--SSSALSEHkkl--ggnGVPLIKVGd--EVINGYNEa----aLR 118 Dechloromonas ar...
ZP_00266350  44 HVVLYATDWCGYCKQTKRFLDq-kGIAFKEFDIEk--DAEARKAYeal--ggrGIPLIDVNg--TLIRGFDPd----eIL 112 Pseudomonas fluo...
ZP_00333697  56 PITLYANACGIPCDQARQLLAe-rGVPFNSKDPQa--SPEGQAELqklt-grlSVPVLVVGt--ERVDGFESg----rWH 125 Thiobacillus den...
ZP_00151620  69 PVTLYTSAECVDGCKQARDLLngrGVPFTEKMLQ---TQAEIDELkqlv-gdaAVPALKVGn--QRFRGFEAa----aYD 138 Dechloromonas ar...
ZP_00502888  87 PVTLYSGENCSPCGAARSMLTs-rGIPFTEKTVTtneDIQALQRLsg----aaSLPFATIGs--QQLKGFSDs----eWT 155 Polaromonas sp. ...

Feature 1          
1H75_A       71 RLH 73  Escherichia coli
AAF11632     74 AFL 76  Deinococcus radiodurans R1
CAC01317     76 QKI 78  Streptomyces coelicolor A3(2)
CAF19548     77 ALA 79  Corynebacterium glutamicum ATCC 13032
NP_962232    77 AKL 79  Mycobacterium avium subsp. paratuberculosis str. k10
ZP_00151591 119 QLL 121 Dechloromonas aromatica RCB
ZP_00266350 113 AAL 115 Pseudomonas fluorescens PfO-1
ZP_00333697 126 TAL 128 Thiobacillus denitrificans ATCC 25259
ZP_00151620 139 NLL 141 Dechloromonas aromatica RCB
ZP_00502888 156 QFL 158 Polaromonas sp. JS666

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