1TKK,1JPD


Conserved Protein Domain Family
L-Ala-DL-Glu_epimerase

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cd03319: L-Ala-DL-Glu_epimerase 
Click on image for an interactive view with Cn3D
L-Ala-D/L-Glu epimerase catalyzes the epimerization of L-Ala-D/L-Glu and other dipeptides. The genomic context and the substrate specificity of characterized members of this family from E.coli and B.subtilis indicates a possible role in the metabolism of the murein peptide of peptidoglycan, of which L-Ala-D-Glu is a component. L-Ala-D/L-Glu epimerase is a member of the enolase-superfamily, which is characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and is stabilized by coordination to the essential Mg2+ ion.
Statistics
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PSSM-Id: 239435
Aligned: 54 rows
Threshold Bit Score: 243.635
Created: 26-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1TKK, B.subtilis L-Ala-D/L-Glu epimerase with bound Mg and dipeptide

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                            
1TKK_A        5 RIETSRIAVPLTKPFKTAlRTVYTAESVIVRITYDsGAVGWGEAPPTLviTGDSMDSIESAIHHv-lKPALlgksla--g 81  Bacillus subtilis
NP_815232     5 QVHVRASKIKLKETFTIAlGTIESADSAIVEIETEeGLVGYGEGGPGIfiTGETLAGTLETIELf--GQAIiglnpf--n 80  Enterococcus fae...
CAE45663      4 RVRLHTAVVPMAAAFDHAtRSRRSAASLLVEIELA-GTRGWGEGAPRDyvTGETLDGAVRAVQAc-dPGELaerie---- 77  Streptomyces par...
CAD77027      5 ELTAFLVPLPLKREIKHAsFTRTESINLLIRCKLSnGTIGWGEGVPRPyvTGETPEGCLQQLSQsnlHPALmqsasnwpe 84  Rhodopirellula b...
ZP_00240269   5 AIHLYAIRLPLRDPFVISyGSYSDMPSIIVKMETDeGTIGYGEGVADDhvTGESWESTFHTLKHt-lAPALigcnpm--n 81  Bacillus cereus ...
NP_469542     5 KIKTHILPIQLSATFKTAlREVSHVNVVRVYIHFDnGIIGIGEAAPTHviTGDTEVSILSAINDi-fGPFLinreld--e 81  Listeria innocua...
AAZ32512      5 DMSWEILEVPLKKPFKIAlGTTTVYQGVLVKLCTD-ELCGYGEAAPSPriTGDTVGSTITALERf--KGALigkdpr--e 79  uncultured eurya...
ZP_00310871   5 QVELYKSPVKLKEPFKISlGILTHANNVIVRIHTAsGHIGYGECSPFMtiHGESMDTAFIVGQYl--AKGLigtscl--d 80  Cytophaga hutchi...
ZP_00387549   5 DLQVDIKQIPLKQPFVTAlHRVDAVNAVRVTVRLAnGVTGVGTCTPNEkvTGDSLSSARAVIEEt-iKPLVlgknlt--d 81  Lactobacillus de...
ZP_00318783   5 DCKLAHYSVSLKMTFKTAlHAQNFANGWIVRLKSEmGNYGYGEVVPSLrvTGDSDASIFGSLKEi-iFPAIigknfe--n 81  Oenococcus oeni ...
Feature 1                                                                            #          
1TKK_A       82 yeailHDIQHLLtg-------nmSAKAAVEMALYDGWAQMCglplyqm---------lggyrdTLETDYTVSVns--pEE 143 Bacillus subtilis
NP_815232    81 iekihEVMDKISaf-------apAAKAAIDIACYDLMGQKAqlplyql---------lggydnQVITDITLGIde--pNV 142 Enterococcus fae...
CAE45663     78 wrdfeSAVASIAqlpltglvdgsSAAAAVEIALLDAVCRHFarpladvlrv---lapparsrrDGPTSVSLVIhlsrdVA 154 Streptomyces par...
CAD77027     85 viaicDALQFNNdsddsrgcgsnSLRCAVELSLLDAFGRHFgqpvsavvphvaeaaeihvrqpRVRYSTTITSsg--aKS 162 Rhodopirellula b...
ZP_00240269  82 iekihDMMDNTIyg-------vpTAKAAIDIACFDIMGKKLnqpvyqli-------ggryheeFPVTHVLSIAep--eAM 145 Bacillus cereus ...
NP_469542    82 eltilDEMKSLLvh-------nsSPKAAIDIALHDALAKAHskplyef---------lggglaGLETDYTISIgt--pEK 143 Listeria innocua...
AAZ32512     80 igklmNELNHLLar-------ntTAKAAVDFALHDILGKTAglpvrnl---------lggerdTIETSLTVTIgs--vED 141 uncultured eurya...
ZP_00310871  81 ivsnsLLMDAIIyg-------nsCIKSAFNIALYDLAAQHAglplyaf--------lggkkdkIIQTDYTVSIde--pHK 143 Cytophaga hutchi...
ZP_00387549  82 wkellNTVKTSIvh-------naPAKAAVEIALYNTRANLFgltlsql---------lggkggSVETDYTISIgk--aEQ 143 Lactobacillus de...
ZP_00318783  82 igkfdDFVSKLItq-------nsAARNAVSEGTLDLFIKDKgtnlesf--------fnlgkgkKVETDYTLSInn--eLN 144 Oenococcus oeni ...
Feature 1                        # #                            #                            #  
1TKK_A      144 MAADAENYLkqg-fqTLKIKVGkddiatdIARIQEIRKRVgsaVKLRLDANQGWrp-kEAVTAIRKMEdaglgIELVEQP 221 Bacillus subtilis
NP_815232   143 MAQKAVEKVklg-fdTLKIKVGtgi-eadIARVKAIREAVgfdIKLRLDANQAWtp-kDAVKAIQALAdy--qIELVEQP 217 Enterococcus fae...
CAE45663    155 TVLDALTPRalaalrHVKIKVAdpa--gaVDRLTAAQDRLpadTRVSLDVNGAWta-eEAEKVAGELDg----VGWVEEP 227 Streptomyces par...
CAD77027    163 ERISALKMRayg-faQCKIKVGvdg-qddPSRVARIRRWIgggVDLRLDANEAWrp-eEVAEKMRPLRef--gVTCIEQP 237 Rhodopirellula b...
ZP_00240269 146 AKEAASMIQkg--yqSFKMKVGtnv-kedVKRIEAVRERVgndIAIRVDVNQGWknsaNTLTALRSLVhl--nIDWIEQP 220 Bacillus cereus ...
NP_469542   144 MVRDAETKVaeg-fqSLKVKLGldpveieVAKIRQMNDALggkIPFRIDANQGWta-dEAIQILNEWQdi--pIDFIEQP 219 Listeria innocua...
AAZ32512    142 SVKSAEELLnag-akVLKVKIGlnp-eedVERIRAIRNITd--AKIRVDANQGYsl-kRAINVLRKIEry--eIEFAEQP 214 uncultured eurya...
ZP_00310871 144 MAADAVQIKkng-feIIKVKVGgsk-eldVERIRMIREAAgdsITLRIDANQGWsv-eTAIETLTLLEpy--nIQHCEEP 218 Cytophaga hutchi...
ZP_00387549 144 MIADAQKMVdqg-fkTLKLKLGaghlkrdIKLVEDLAYAAgpmVHLRLDMNQACdv-rQTMQAAEYWHkqgllIDFIEQP 221 Lactobacillus de...
ZP_00318783 145 MLNEAKKLSkkg-fsTLKIKLGddliekeVQKLVYLNNHLe-nISFRIDANQAWnl-rGSLRFDQLAFenrlpIETIEQP 221 Oenococcus oeni ...
Feature 1                             #                                                   # #   
1TKK_A      222 VhkddLAGLKKvtdatdTPIMADESVftpRQAFEVLqtrSADLINIKLMKAgGISGAEKINAMAeacgVECMVGSMI-ET 300 Bacillus subtilis
NP_815232   218 VkrrdLEGLKYvtsqvnTTIMADESCfdaQDALELVkkgTVDVINIKLMKCgGIHEALKINQICetagIECMIGCMAeET 297 Enterococcus fae...
CAE45663    228 LpprsWPELGRlrratgLPVMLDESCtgpADLHAAAtsgAASHINVRLSKCgGFLAAARLALRAdelgVGCQLGVHVaEV 307 Streptomyces par...
CAD77027    238 VahdqVDALASlresigVPIMLDESLtsrMDAERAIelgTCDLFNLRLSKCgGFLSCLRLAALArasgLGYQLGCHPgES 317 Rhodopirellula b...
ZP_00240269 221 VvaddIDAMAHirsktdLPLMIDEGLkgsREMRQIIkldAADKVNIKLMKCgGIYPAVKLAHQAemagIECQVGSMV-ES 299 Bacillus cereus ...
NP_469542   220 VkswdFTGMAKvtantsFPIMADESLfgfHDAKRLLdekCCNLLNIKLMKSaGIKEAQAIHDLAgsynVDCMIGTMI-EG 298 Listeria innocua...
AAZ32512    215 LpaaqVEELGVlrrsteIPIMADEAVhtkEDVLRLVg--KVDAINIKLMKSgGIYEAARMAAVAkaagMKIMVGCMI-ET 291 uncultured eurya...
ZP_00310871 219 VsrnlYTALPKirqacrIPIMADESCcnsFDAERLIqiqACDSFNLKLSKSaGITNALNIIRLAeqahMPVQVGGFL-ES 297 Cytophaga hutchi...
ZP_00387549 222 VpawdLDSMAYltrhspYPIMADESVesyEDAQRVLaknACDYINIKLMKTgGLSEAQKINDLAesrgVPTMVGSMI-ES 300 Lactobacillus de...
ZP_00318783 222 LpigfEDEYSYligkfhFPIILDESVhefNDARKLNpgvDFNGYNVKLEKSgGISQAKALLDYSesldKNTMIGCMI-ES 300 Oenococcus oeni ...
Feature 1                                # #     
1TKK_A      301 KLGITAAAHFAaskr-----nitRFDFDAPLML 328 Bacillus subtilis
NP_815232   298 TIGITAAAHLAaaqk-----nitRADLDATFGL 325 Enterococcus faecalis V583
CAE45663    308 GPLWAAGRTLAtawdlwqtveagRADEWFPVPL 340 Streptomyces parvulus
CAD77027    318 GVLSAAGRHWAssv-------rdIRYLEGSYDR 343 Rhodopirellula baltica SH 1
ZP_00240269 300 SVASSAGFHVAfskk-----iitSVELTGPLKF 327 Bacillus cereus G9241
NP_469542   299 YASLSAAAHFAaask-----qvkFYDLDVPFMW 326 Listeria innocua Clip11262
AAZ32512    292 RLGITAGTHFAlgv------gvdYADLDGYWDL 318 uncultured euryarchaeote Alv-FOS4
ZP_00310871 298 RLGFTAAAHVAlvsk-----ticYYDFDTPLMF 325 Cytophaga hutchinsonii
ZP_00387549 301 IESLAAACAFYranp-----ntiFADLDAIFMV 328 Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365
ZP_00318783 301 NIGIAFAAALAkayp-----ivkFIDLDSPLMF 328 Oenococcus oeni PSU-1

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