GEO DataSets

(Submitter supplied) Murine erythroleukemia (MEL) cells are differentiated by dimethyl sulfoxide (DMSO), hexamethylene bisacetamide (HMBA), or trichostatin A (TSA) treatment. We analyzed expression profiles of high differentiation-inducible (HD) MEL cells during chemical induced differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16035

60 Samples

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(Submitter supplied) Murine erythroleukemia (MEL) cells are differentiated by dimethyl sulfoxide (DMSO）, hexamethylene bisacetamide (HMBA） or trichostatin A (TSA） treatment. We selsected high differentiation-inducible (HD) and low differentiation-inducible (LD)-MEL cells by recloning of original MEL cells. We screened erythroid differentiation related-genes to compare transcriptome of HD and LD-MEL cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16035

48 Samples

Download data: TXT

(Submitter supplied) Each cDNA was extracted from cDNA library by Wizard SV 96 Plasmid DNA Purification System (Promega) and amplified by polymerase chain reaction (PCR) using Taq polymerase (Promega). PCR products were purified by isopropanol precipitation and resuspended in 50% DMSO, and spotted to GAPS II Coated Slides (Corning) using Micro Grid II (BioRobotics). After spotting, cDNAs were anchored to slide glasses by heat denaturation at 100°C and UV cross-linking (200 mJ).

Organism:

Mus musculus

2 Series

108 Samples

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