Each cDNA was extracted from cDNA library by Wizard SV 96 Plasmid DNA Purification System (Promega) and amplified by polymerase chain reaction (PCR) using Taq polymerase (Promega). PCR products were purified by isopropanol precipitation and resuspended in 50% DMSO, and spotted to GAPS II Coated Slides (Corning) using Micro Grid II (BioRobotics). After spotting, cDNAs were anchored to slide glasses by heat denaturation at 100°C and UV cross-linking (200 mJ).
Comparison of transcriptome of high differentiation-inducible murine erythroleukemia cells and low differentiation-inducible murine erythroleukemia cells
Expression profiles of high differentiation-inducible murine erhthroleukemia cells
Data table header descriptions
ID
Daughter
Rearrayed clone name
Parent
Source clone name
Clone Verification
Either the 3' sequence or the 5' sequence show sufficient homology to verify the address of the clone is correct. This field has a link to the sequence verification pages (with an arrow pointing at your clone of interest).
FullSeq
Value='Y' indicates full sequence for the clone is available. 3' sequence and 5' sequence assembled using Phred/Phrap system. This field has a link to the sequence pages for the cDNAs of interest.
Sequence Name (3')
3' sequence of the Daughter Clone
3' Sequence verified
The 3' sequence of the Daughter clone shows sufficient homology to the 3' parent clone to verify the sequence. Values are 'Y'= verified, 'U'= Unvarifiable, <blank>=not verified, 'O'=Orphan. An Orphan is any clone which has been sequence verified by multiple sequences obtained from the daughter clones, but does not match the sequence of the parent clone.
Length
Length of 3' sequence of the Daughter Clone
Genbank accession number (3')
GenBank Accession number of the 3' end Sequence of the Daughter Clone
3' Putative Gene ID based on NIA Mouse Gene Index version 4
The 3' Sequence Description is taken based on NIA Mouse Gene Index version 4 clustering. For more information please visit NIA Mouse Gene Index version 4 at http://lgsun.grc.nia.nih.gov/geneindex4/.
3' Gene Symbol(s) based on NIA Mouse Gene Index version 4 'U' cluster
Gene symbol(s) of NIA Mouse Gene Index version 4 cluster in which 3' sequence present.
NIA Mouse Gene Index version 4 cluster
NIA Mouse Gene Index version 4 cluster in which 3' Sequence present.
Chromosome based on NIA Mouse Gene Index version4 'U' cluster
NIA Mouse Gene Index version 4 cluster chromosome in which 3' sequence present.
Strand information based on NIA Mouse Gene Index version 4 'U' cluster
NIA Mouse Gene Index version 4 cluster chromosome strand in which 3' sequence present.
3' sequence 'U' cluster chromosome start position based NIA Mouse Gene Index version 4
NIA Mouse Gene Index version 4 cluster chromosome start position in which 3' sequence present.
3' sequence 'U' cluster chromosome end position based NIA Mouse Gene Index version 4
NIA Mouse Gene Index version 4 cluster chromosome end position in which 3' sequence present.
Sequence Name (5')
5' sequence of the Daughter Clone
5' Sequence verified
The 5' sequence of the Daughter clone shows sufficient homology to the 5' parent clone to verify the sequence. Values are 'Y'= verified, 'U'= Unvarifiable, <blank>=not verified, 'O'=Orphan. An Orphan is any clone which has been sequence verified by multiple sequences obtained from the daughter clones, but does not match the sequence of the parent clone.
Length
Length of 5' sequence of the Daughter Clone
Genbank accession number (5')
GenBank Accession number of the 5' end Sequence of the Daughter Clone
5' Putative Gene ID based on NIA Mouse Gene Index version 4
The 5' Sequence Description is taken based on NIA Mouse Gene Index version 4 clustering. For more information please visit NIA Mouse Gene Index version 4 at http://lgsun.grc.nia.nih.gov/geneindex4/.
5' Gene Symbol(s) based on NIA Mouse Gene Index version 4 'U' cluster
Gene symbol(s) of NIA Mouse Gene Index version 4 cluster in which 5' sequence present.
NIA Mouse Gene Index version 4 cluster
NIA Mouse Gene Index version 4 cluster in which 5' Sequence present.
Chromosome based on NIA Mouse Gene Index version 4 'U' cluster
NIA Mouse Gene Index version 4 cluster chromosome in which 5' sequence present.
Strand information based on NIA Mouse Gene Index version 4 'U' cluster
NIA Mouse Gene Index version 4 cluster chromosome strand in which 5' sequence present.
5' sequence 'U' cluster chromosome start position based NIA Mouse Gene Index version 4
NIA Mouse Gene Index version 4 cluster chromosome start position in which 5' sequence present.
5' sequence 'U' cluster chromosome end position based NIA Mouse Gene Index version 4
NIA Mouse Gene Index version 4 cluster chromosome end position in which 5' sequence present.
GB_ACC
GenBank accession
CLONE_ID
Data table
ID
Daughter
Parent
Clone Verification
FullSeq
Sequence Name (3')
3' Sequence verified
Length
Genbank accession number (3')
3' Putative Gene ID based on NIA Mouse Gene Index version 4
3' Gene Symbol(s) based on NIA Mouse Gene Index version 4 'U' cluster
NIA Mouse Gene Index version 4 cluster
Chromosome based on NIA Mouse Gene Index version4 'U' cluster
Strand information based on NIA Mouse Gene Index version 4 'U' cluster
3' sequence 'U' cluster chromosome start position based NIA Mouse Gene Index version 4
3' sequence 'U' cluster chromosome end position based NIA Mouse Gene Index version 4
Sequence Name (5')
5' Sequence verified
Length
Genbank accession number (5')
5' Putative Gene ID based on NIA Mouse Gene Index version 4
5' Gene Symbol(s) based on NIA Mouse Gene Index version 4 'U' cluster
NIA Mouse Gene Index version 4 cluster
Chromosome based on NIA Mouse Gene Index version 4 'U' cluster
Strand information based on NIA Mouse Gene Index version 4 'U' cluster
5' sequence 'U' cluster chromosome start position based NIA Mouse Gene Index version 4
5' sequence 'U' cluster chromosome end position based NIA Mouse Gene Index version 4