GSM1066117[Accession] - GEO DataSets

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Items: 4

Select item 2000436051.

Distribution of elongating and total RNA polymerase II in xrn1 mutants using GRO and RPCC

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array; Genome binding/occupancy profiling by array

Platform:: GPL16503
22 Samples

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Accession:: GSE43605

ID:: 200043605

Select item 2000436022.

Distribution of elongating RNA polymerase II along the 5'/3' regions in xrn1 mutants

(Submitter supplied) Determination of the 3' or 5' intragenic nascent transcriptional rate. Maintaining the proper mRNA levels is a key aspect in the regulation of gene expression. The balance between mRNA synthesis and decay determines these levels. Using a whole-genome analysis, we demonstrate that most yeast mRNAs are degraded by the 5'-to-3' pathway (the "decaysome"), as proposed previously. Unexpectedly, the level of these mRNAs is highly robust to perturbations in this major pathway, as defects in various decaysome components lead to transcription down-regulation. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL16503
12 Samples

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Series

Accession:: GSE43602

ID:: 200043602

Select item 1000165033.

Valencia yeast 3' 5' (384)

(Submitter supplied) Nylon macroarray (7 x 11 cm) with 768 positions: 754 single spotted of about 300 bp 3' or 5' ORF end region, plus 12 probes for rRNA controls and 2 empty spots. Protocol: See Rodríguez-Gil et al. (2010), Nucleic Acids Research Vol. 38 No. 14. pp. 4651-4664. PMID 20385590. doi: 10.1093/nar/gkq215.