GSM174423[Accession] - GEO DataSets

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Items: 3

Select item 2000072301.

Genome-wide assessment of DNA aberrations in human neuroblastoma primary tumors using BAC array

(Submitter supplied) DNA signature patterns embed important prognostic information in diagnostic neuroblastoma samples, and can identify candidate cancer-related genes. Keywords: aCGH

Organism:: Homo sapiens

Type:: Genome variation profiling by genome tiling array

Platform:: GPL4894
82 Samples

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Accession:: GSE7230

ID:: 200007230

Analyze with GEO2R

Select item 1000048942.

Penn/CHOP Homo sapiens 4.5K (BAC array for studying Neuroblastoma)

(Submitter supplied) BAC clone starter cultures were grown in 700ul 2X YT broth with 12.5 ug/mL chloramphenicol at 37 deg C for ~15h. Full cultures of 2.5 mL broth were inoculated from starter cultures and grown for 15 h in 48-well blocks. DNA was extracted using QAGEN real prep kits using high throuhput 96 well blocks. BAC DNA was then amplified using degenerate oligonucliotide primers (Cheung and Nelson 1996). Each BAC was amplified twice at different annealing temperatures (58 and 60 deg C). more...