GEO DataSets

(Submitter supplied) Tumor-derived cell lines are used as in vitro cancer models, but their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line–specific cytogenetic changes. We have addressed the issue of whether copy number alterations seen in tumor-derived cell lines reflect those observed in studies of fresh tissue by carrying out a meta-analysis of array-based comparative genomic hybridization data that considers both copy number alteration frequencies and the occurrence of cancer gene amplifications and homozygous deletions. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL4894

624 Samples

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(Submitter supplied) BAC clone starter cultures were grown in 700ul 2X YT broth with 12.5 ug/mL chloramphenicol at 37 deg C for ~15h. Full cultures of 2.5 mL broth were inoculated from starter cultures and grown for 15 h in 48-well blocks. DNA was extracted using QAGEN real prep kits using high throuhput 96 well blocks. BAC DNA was then amplified using degenerate oligonucliotide primers (Cheung and Nelson 1996). Each BAC was amplified twice at different annealing temperatures (58 and 60 deg C). more...

Organism:

Homo sapiens

3 Series

716 Samples

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