GSM71239[Accession] - GEO DataSets

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Items: 3

Select item 2000031711.

DnaA coordinates replication initiation and cell cycle transcription in Caulobacter crescentus

(Submitter supplied) Supporting data for Hottes et al., "DnaA coordinates replication initiation and cell cycle transcription in Caulobacter crescentus" The microarray component of this work monitors mRNA expression during the cell cycle of synchronized populations of Caulobacter crescentus cells. Transcription during the normal cell cycle is compared with transcription during a cell cycle where expression of dnaA, which encodes a key DNA replication initiation factor, is delayed. more...

Organism:: Caulobacter vibrioides

Type:: Expression profiling by array

Platforms:: GPL1076 GPL2748 GPL2749
83 Samples

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Accession:: GSE3171

ID:: 200003171

Select item 1000027492.

Stanford Caulobacter crescentus printing Z

(Submitter supplied) Spotted oligonucleotide array on aldehyde coated glass. The array contains at least 1 50-mer probe for each ORF in Caulobacter crescentus. Protocol: The microarrays for this study were printed using the GeneMachines Omnigrid Microarrayer with Telechem 16-pin ArrayIt Stealth MicroSpotting Pins. Oligo sequences were chosen to satisfy the following criteria: be a unique sequence in the genome, be located near the 5’ end of the gene, approximately 100 base pairs downstream of the predicted start codon, have similar melting temperatures, and have minimal predicted secondary structure formation. more...