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Status
Public on Dec 08, 2005
Title
Stanford Caulobacter crescentus printing Z
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Caulobacter vibrioides Manufacturer
Stanford University
Manufacture protocol
The microarrays for this study were printed using the GeneMachines Omnigrid Microarrayer with Telechem 16-pin ArrayIt Stealth MicroSpotting Pins. Oligo sequences were chosen to satisfy the following criteria: be a unique sequence in the genome, be located near the 5’ end of the gene, approximately 100 base pairs downstream of the predicted start codon, have similar melting temperatures, and have minimal predicted secondary structure formation. The oligos were modified at the 5’ end with a six-carbon spacer followed by a primary amino group and diluted to 20µM for printing. Aldehydesilane coated slides (Schott Nexterion-slide AL) and QMT Spotting Solution I (Quantifoil) were used. Arrays use the same oligos as those in Platform GPL1076.
Description
Spotted oligonucleotide array on aldehyde coated glass.
Web link
http://www.stanford.edu/group/caulobacter/oligoArrays/ Contributor(s)
Hottes AK , McAdams HH , Meewan M Submission date
Aug 12, 2005
Last update date
Apr 13, 2011
Contact name
Alison Hottes
Organization name
Princeton University
Department
Lewis-Sigler Institute for Integrative Genomics
Street address
220 Carl Icahn Lab Washington Road
City
Princeton
State/province
NJ
ZIP/Postal code
08544
Country
USA
Samples (37)
GSM70405 , GSM70406 , GSM70407 , GSM70408 , GSM70413 , GSM70414
GSM70415 ,
GSM70416 ,
GSM70417 ,
GSM71217 ,
GSM71218 ,
GSM71219 ,
GSM71220 ,
GSM71221 ,
GSM71222 ,
GSM71223 ,
GSM71224 ,
GSM71225 ,
GSM71226 ,
GSM71227 ,
GSM71228 ,
GSM71229 ,
GSM71230 ,
GSM71231 ,
GSM71232 ,
GSM71233 ,
GSM71234 ,
GSM71235 ,
GSM71236 ,
GSM71237 ,
GSM71238 ,
GSM71239 ,
GSM71240 ,
GSM71241 ,
GSM71242 ,
GSM311586 ,
GSM311587 Series (3)
GSE3144 Cytokinesis signals truncation of the PodJ polarity factor by a cell cycle-regulated protease
GSE3171 DnaA coordinates replication initiation and cell cycle transcription in Caulobacter crescentus
GSE12414 Caulobacter vibrioides CB15N mRNA expression in M2-Glucose Vs. M2-Inositol
Data table header descriptions ID ORF Open reading frame identifier
SPOT_ID Identifier for features that do not represent an open reading frame
OLIGO_ID Unique identifier of oligonucleotide sequence
SEQUENCE Sequence of oligonucleotide probe
TIP Printing tip used (block number)
ROW Row within tip's block
COL Column within tip's block
Data table ID ORF SPOT_ID OLIGO_ID SEQUENCE TIP ROW COL 149075
16S rRNA
11119
GCGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGATCCTTCGG
1
12
17
149077
23S rRNA
11120
GCGCATAAGGGCTTCTGACGGATGCCTTGGCATTGAGAGGCGATGAAGGA
9
12
18
149079
5S rRNA
11121
ACCTGGTGGCTATGCCGGGGGTTCCCCACCCGATCCCATTCCGAACTCGG
1
12
18
145416
CC0001 11138
GAGCGAAAGGCTGCCGCCACGCTTCGCCACCTACTTCCATATCCACTTGG
13
1
1
145418
CC0002 11139
GGCCGAAGACGTCACGCCGCGCGACATCGCCGACGCCCTGGCCGAGATGA
5
1
1
145420
CC0003 11140
TGGATCGCAGCGGCCGGCCTTGACGCGGCTTATGTGCCATTCGCCCCGGC
13
1
2
145422
CC0004 11141
GGCTGACCGGTTCCATCGGCATGGGCAAGTCGACGACCTCCAAGATGTTC
5
1
2
145424
CC0005 11142
ATCGAAGTGGTCGATTTCATGCCGACCGGCCGCTCGTTCCACGAGTACTG
13
1
3
145426
CC0006 11143
GGCCAAGGCCTTCGCCGCCGGCGCCGACATCAAAGAGATGTCGGACAAGA
5
1
3
145428
CC0007 11144
GACGCGATCGCCAAGGGCGATGTCGCCGTCGCCAAGGCGGCTTTCGTGGA
13
1
4
145430
CC0008 11145
GAAGGTTTGCGTGGCTTTGAAGCGTGAGCTGGGTGACGCGGCCTTCGGGT
5
1
4
149087
CC0008 11146
GGCCTTGCGCGCTGAGGACAGCGCCTTGAGCCACGATCTGGAGACCCTGA
1
12
20
145432
CC0009 11147
TACGGGAAGCCGATGAGCGTTGCGATGACGGCGCTTGGGTCGCTGGGCTG
13
1
5
145434
CC0010 11148
CGAGGGCGCTCGCACCACCCCGTCGGTCGTCGCCTTCCTCGAGGACGGTG
5
1
5
145436
CC0011 11149
TGGCGATGGAACACCACCCTGACCGCAATGGCGGCTGCGAGAACGCGGCC
13
1
6
145438
CC0012 11150
GCGCTGATCTTCTTCCGCATGGGCGATTTCTACGAGCTGTTCTTCGACGA
5
1
6
149088
CC0012 11151
GCCCGTGGTGGTGCGCGCCCGCGAGGTGCTGGATCGGCTGGAGAGCAAGG
14
12
15
145464
CC0013 11152
CCTCGACTCGATCGGTAATGAGGCCGAGCAGCGGGCCCGCGCCATCGACA
15
1
1
145466
CC0014 11153
GCGCGGTGGCCGAACTGGCCGGTGTGATGGCCGCCAAGAAGACGTCCGAC
7
1
1
145468
CC0015 11154
TGGTGGTCCGCGACGACATCGAGCAGATCAGGGCCCAGGTGCGCTCTTGG
15
1
2
3938 304 Kbytes .Supplementary data files not provided
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